Applied Microbiology and Biotechnology 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Recent advances in microbial synthesis of free heme
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-11. doi: 10.1007/s00253-023-12968-5. Epub 2024 Jan 9.ABSTRACTHeme is an iron-containing porphyrin compound widely used in the fields of healthcare, food, and medicine. Compared to animal blood extraction, it is more advantageous to develop a microbial cell factory to produce heme. However, heme biosynthesis in microorganisms is tightly regulated, and its accumulation is highly cytotoxic. The current review describes the biosynthetic pathway of free heme, its fermentation production using different engineered bacteria constructed by metabolic engineering, and strategies for furth...
Source: Applied Microbiology and Biotechnology - January 9, 2024 Category: Microbiology Authors: Shaomei Yang Zihao Guo Jiuyu Sun Jingxuan Wei Qinyuan Ma Xiuzhen Gao Source Type: research
Expression of virus-like particles (VLPs) of foot-and-mouth disease virus (FMDV) using Saccharomyces cerevisiae
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-10. doi: 10.1007/s00253-023-12902-9. Epub 2024 Jan 9.ABSTRACTWe engineered Saccharomyces cerevisiae to express structural proteins of foot-and-mouth disease virus (FMDV) and produce virus-like particles (VLPs). The gene, which encodes four structural capsid proteins (VP0 (VP4 and VP2), VP3, and VP1), followed by a translational "ribosomal skipping" sequence consisting of 2A and protease 3C, was codon-optimized and chemically synthesized. The cloned gene was used to transform S. cerevisiae 2805 strain. Western blot analysis revealed that the polyprotein consisting of VP0, VP3, an...
Source: Applied Microbiology and Biotechnology - January 9, 2024 Category: Microbiology Authors: Ngoc My Tieu Le Kum-Kang So Jeesun Chun Dae-Hyuk Kim Source Type: research
Optimization of welan gum extraction and purification using lysozyme and alkaline protease
In this study, we established a method for the extraction and purification of welan gum using a synergistic approach with lysozyme and alkaline protease. Lysozyme hydrolysis conditions were optimized by applying response surface methodology, and the best results for bacterial cell removal were achieved at 11 000 U/g, 44 °C, and pH 9 after 3 h of treatment. Subsequently, we evaluated protein hydrolysis through computer simulation and identified alkaline protease as the most suitable enzyme. Through experimental investigations, we found that the optimal conditions for alkaline protease hydrolysis were 7500 U/g, 50 °C, pH 1...
Source: Applied Microbiology and Biotechnology - January 9, 2024 Category: Microbiology Authors: Yuying Wang TianTian Zhang Li Zhu Ruotong Li Yun Jiang Zhitao Li Minjie Gao Xiaobei Zhan Source Type: research
Metabolic engineering of Caldicellulosiruptor bescii for hydrogen production
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-10. doi: 10.1007/s00253-023-12974-7. Epub 2024 Jan 9.ABSTRACTHydrogen is an alternative fuel for transportation vehicles because it is clean, sustainable, and highly flammable. However, the production of hydrogen from lignocellulosic biomass by microorganisms presents challenges. This microbial process involves multiple complex steps, including thermal, chemical, and mechanical treatment of biomass to remove hemicellulose and lignin, as well as enzymatic hydrolysis to solubilize the plant cell walls. These steps not only incur costs but also result in the production of toxic hyd...
Source: Applied Microbiology and Biotechnology - January 9, 2024 Category: Microbiology Authors: Minseok Cha Jung Kon Kim Won-Heong Lee Hyoungwoon Song Tae-Gi Lee Sun-Ki Kim Soo-Jung Kim Source Type: research
Synthesis and application of a phenazine class substrate for high-throughput screening of laccase activity
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-12. doi: 10.1007/s00253-023-12958-7. Epub 2024 Jan 9.ABSTRACTBiocatalysis is one of the greatest tools for implementing the 12 principles of Green chemistry. Biocatalysts are bio-based, highly efficient and selective, operate at moderate conditions, and can be reused multiple times. However, the wider application of biocatalysts is plagued by a plethora of drawbacks, such as poor stability at operating conditions, inadequate efficiency of catalytic systems, a small number of commercially available biocatalysts, and a lack of substrates or methods for their discovery and developm...
Source: Applied Microbiology and Biotechnology - January 9, 2024 Category: Microbiology Authors: Justinas Babinskas Jerica Saboti č Inga Matijo šytė Source Type: research
Adaptive laboratory evolution for improved tolerance of vitamin K in Bacillus subtilis
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-13. doi: 10.1007/s00253-023-12877-7. Epub 2024 Jan 9.ABSTRACTMenaquinone-7 (MK-7), a subtype of vitamin K2 (VK2), assumes crucial roles in coagulation function, calcium homeostasis, and respiratory chain transmission. The production of MK-7 via microbial fermentation boasts mild technological conditions and high biocompatibility. Nevertheless, the redox activity of MK-7 imposes constraints on its excessive accumulation in microorganisms. To address this predicament, an adaptive laboratory evolution (ALE) protocol was implemented in Bacillus subtilis BS011, utilizing vitamin K3 (...
Source: Applied Microbiology and Biotechnology - January 9, 2024 Category: Microbiology Authors: Xiumin Ding Zhiming Zheng Genhai Zhao Li Wang Han Wang Peng Wang Source Type: research
A novel conserved B-cell epitope in pB602L of African swine fever virus
This study found one novel conserved B-cell epitope 474SKENLTPDE482. • In the 3D structure, 474SKENLTPDE482 is exposed on the surface of ASFV pB602L, forming a curved linear structure.PMID:38194141 | DOI:10.1007/s00253-023-12921-6 (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - January 9, 2024 Category: Microbiology Authors: Jinxing Song Mengxiang Wang Lei Zhou Panpan Tian Junru Sun Zhuoya Sun Chenyun Guo Yanan Wu Gaiping Zhang Source Type: research
Vaginal prevention of Candida albicans: synergistic effect of lactobacilli and mannan oligosaccharides (MOS)
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-17. doi: 10.1007/s00253-023-12909-2. Epub 2024 Jan 9.ABSTRACTVulvovaginal candidiasis (VVC) affects approximately 30-50% of women at least once during their lifetime, causing uncomfortable symptoms and limitations in their daily quality of life. Antifungal therapy is not very effective, does not prevent recurrencies and usually causes side effects. Therefore, alternative therapies are urgently needed. The goal of this work was to investigate the potential benefits of using mannan oligosaccharides (MOS) extracts together with a Lactobacillus sp. pool, composed by the most signifi...
Source: Applied Microbiology and Biotechnology - January 9, 2024 Category: Microbiology Authors: Margarida Faustino Joana Odila Pereira Ana Margarida Pereira Ana Sofia Oliveira Carlos M H Ferreira Carla F Pereira Joana Dur ão Manuela E Pintado Ana P Carvalho Source Type: research
Evaluation of selected tropical marine microalgal cultures for use in biophotovoltaic platforms
In this study, the bioelectrical power generation potential of four tropical marine microalgal strains native to Malaysia was investigated using BPV platforms. Chlorella UMACC 258 produced the highest power density (0.108 mW m-2), followed by Halamphora subtropica UMACC 370 (0.090 mW m-2), Synechococcus UMACC 371 (0.065 mW m-2) and Parachlorella UMACC 245 (0.017 mW m-2). The chlorophyll-a (chl-a) content was examined to have a linear positive relationship with the power density (p < 0.05). The photosynthetic performance of strains was studied using the pulse-amplitude modulation (PAM) fluorometer; parameters measured in...
Source: Applied Microbiology and Biotechnology - January 9, 2024 Category: Microbiology Authors: Zoe Hui-Yee Tay Fong-Lee Ng Cheng-Han Thong Choon-Weng Lee G Gnana Kumar Abdullah G Al-Sehemi Siew-Moi Phang Source Type: research
New bacteriophage-derived lysins, LysJ and LysF, with the potential to control Bacillus anthracis
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-14. doi: 10.1007/s00253-023-12839-z. Epub 2024 Jan 9.ABSTRACTBacillus anthracis is an etiological agent of anthrax, a severe zoonotic disease that can be transmitted to people and cause high mortalities. Bacteriophages and their lytic enzymes, endolysins, have potential therapeutic value in treating infections caused by this bacterium as alternatives or complements to antibiotic therapy. They can also be used to identify and detect B. anthracis. Endolysins of two B. anthracis Wbetavirus phages, J5a and F16Ba which were described by us recently, differ significantly from the best...
Source: Applied Microbiology and Biotechnology - January 9, 2024 Category: Microbiology Authors: Aleksandra Nakonieczna Agnieszka Topolska-Wo ś Ma łgorzata Łobocka Source Type: research
Engineering the Tat-secretion pathway of Bacillus licheniformis for the secretion of cytoplasmic enzyme arginase
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-12. doi: 10.1007/s00253-023-12917-2. Epub 2024 Jan 9.ABSTRACTThe industrial bacterium Bacillus licheniformis has long been used as a microbial factory for the production of enzymes due to its ability to secrete copious amounts of native extracellular proteins and its generally regarded as safe (GRAS) status. However, most attempts to use B. licheniformis to produce heterologous and cytoplasmic enzymes primarily via the general secretory (Sec) pathway have had limited success. The twin-arginine transport (Tat) pathway offers a promising alternative for the extracellular export of...
Source: Applied Microbiology and Biotechnology - January 9, 2024 Category: Microbiology Authors: Yi Ji Junliang Li Yonglin Liang Liang Li Yajun Wang Li Pi Panpan Xing Christopher T Nomura Shouwen Chen Chengjun Zhu Qin Wang Source Type: research
Milk microbiome transplantation: recolonizing donor milk with mother's own milk microbiota
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-10. doi: 10.1007/s00253-023-12965-8. Epub 2024 Jan 9.ABSTRACTDonor human milk (DHM) provides myriad nutritional and immunological benefits for preterm and low birthweight infants. However, pasteurization leaves DHM devoid of potentially beneficial milk microbiota. In the present study, we performed milk microbiome transplantation from freshly collected mother's own milk (MOM) into pasteurized DHM. Small volumes of MOM (5%, 10%, or 30% v/v) were inoculated into pasteurized DHM and incubated at 37 °C for up to 8 h. Further, we compared microbiome recolonization in UV-C-treated an...
Source: Applied Microbiology and Biotechnology - January 9, 2024 Category: Microbiology Authors: Lisa F Stinson Jie Ma Ching Tat Lai Alethea Rea Sharon L Perrella Donna T Geddes Source Type: research
A previously unidentified sugar transporter for engineering of high-yield Streptomyces
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-13. doi: 10.1007/s00253-023-12964-9. Epub 2024 Jan 9.ABSTRACTSugar transporters have significant contributions to regulate metabolic flux towards products and they are general potential targets for engineering of high-yield microbial cell factories. Streptomyces, well-known producers of natural product pharmaceuticals, contain an abundance of sugar transporters, while few of them are well characterized and applied. Here, we report a previously unidentified ATP-binding cassette (ABC) sugar transporter TP6568 found within a Streptomyces avermitilis transposon library, along with i...
Source: Applied Microbiology and Biotechnology - January 9, 2024 Category: Microbiology Authors: Zhuoxu Dong Lei Li Guozhong Du Yanyan Zhang Xiangjing Wang Shanshan Li Wensheng Xiang Source Type: research
Development and evaluation of a test strip for the rapid detection of antibody against equine infectious anemia virus
In this study, we designed and developed a colloidal gold immunochromatographic (GICG) test strip to detect antibodies against EIAV based on the double-antigen sandwich. Both the p26 and gp45 proteins were used as the capture antigens, which may help to improve the positive detection rate of the strip. We found that the sensitivity of the test strip was 8 to 16 times higher than those of two commercially available ELISA tests and 128 to 256 times higher than AGID, but 8 to 16 times lower than that of western blotting. The strip has good specificity and stability. When serum samples from experimental horses immunized with t...
Source: Applied Microbiology and Biotechnology - January 8, 2024 Category: Microbiology Authors: Zenan Zhang Kui Guo Xiaoyu Chu Mingru Liu Cheng Du Zhe Hu Xiaojun Wang Source Type: research
Characterization and stabilization of GluLm and its application to deglycosylate dietary flavonoids and lignans
This study describes the characterization of the recombinant GH3 aryl-β-glucosidase "GluLm" from Limosilactobacillus mucosae INIA P508, followed by its immobilization on an agarose support with the aim of developing an efficient application to increase the availability and concentration of flavonoid and lignan aglycones in a vegetal beverage. In previous studies, heterologous GluLm-producing strains demonstrated a great capacity to deglycosylate flavonoids. Nevertheless, the physicochemical properties and substrate spectrum of the enzyme remained unknown up to now. A high production of purified GluLm was achieved (14 mg L...
Source: Applied Microbiology and Biotechnology - January 8, 2024 Category: Microbiology Authors: Jos é Antonio Curiel Ana Ruiz de la Bastida Susana Langa Ángela Peirotén Jos é María Landete Source Type: research
