Applied Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

X-ray structure and characterization of a probiotic Lactobacillus rhamnosus Probio-M9 L-rhamnose isomerase
Appl Microbiol Biotechnol. 2024 Mar 2;108(1):249. doi: 10.1007/s00253-024-13075-9.ABSTRACTA recombinant L-rhamnose isomerase (L-RhI) from probiotic Lactobacillus rhamnosus Probio-M9 (L. rhamnosus Probio-M9) was expressed. L. rhamnosus Probio-M9 was isolated from human colostrum and identified as a probiotic lactic acid bacterium, which can grow using L-rhamnose. L-RhI is one of the enzymes involved in L-rhamnose metabolism and catalyzes the reversible isomerization between L-rhamnose and L-rhamnulose. Some L-RhIs were reported to catalyze isomerization not only between L-rhamnose and L-rhamnulose but also between D-allulos...
Source: Applied Microbiology and Biotechnology - March 2, 2024 Category: Microbiology Authors: Hiromi Yoshida Naho Yamamoto Lin Hai Kurahara Ken Izumori Akihide Yoshihara Source Type: research

Acremonium sp. diglycosidase-aid chemical diversification: valorization of industry by-products
Appl Microbiol Biotechnol. 2024 Mar 2;108(1):250. doi: 10.1007/s00253-023-12957-8.ABSTRACTThe fungal diglycosidase α-rhamnosyl-β-glucosidase I (αRβG I) from Acremonium sp. DSM 24697 catalyzes the glycosylation of various OH-acceptors using the citrus flavanone hesperidin. We successfully applied a one-pot biocatalysis process to synthesize 4-methylumbellipheryl rutinoside (4-MUR) and glyceryl rutinoside using a citrus peel residue as sugar donor. This residue, which contained 3.5 % [w/w] hesperidin, is the remaining of citrus processing after producing orange juice, essential oil, and peel-juice. The low-cost compound ...
Source: Applied Microbiology and Biotechnology - March 2, 2024 Category: Microbiology Authors: Micaela Baglioni Alexander Fries Jan-Mathis M üller Alejandra Omarini Michael M üller Javier D Breccia Laura S Mazzaferro Source Type: research

Assessing immunogenicity of CRISPR-NCas9 engineered strain against porcine epidemic diarrhea virus
In this study, we used the CRISPR-NCas9 system to integrate the PEDV S1 gene into the genome of alanine racemase-deficient Lactobacillus paracasei △Alr HLJ-27 (L. paracasei △Alr HLJ-27) at the thymidylate synthase (thyA) site, generating a strain, S1/△Alr HLJ-27. We conducted immunization assays in mice and piglets to evaluate the level of immune response and evaluated its protective effect against PEDV through challenge tests in piglets. Oral administration of the strain S1/△Alr HLJ-27 in mice and piglets elicited mucosal, humoral, and cellular immune responses. The strain also exhibited a certain level of resista...
Source: Applied Microbiology and Biotechnology - March 2, 2024 Category: Microbiology Authors: Fengsai Li Haiyuan Zhao Ling Sui Fangjie Yin Xinzi Liu Guihai Guo Jiaxuan Li Yanping Jiang Wen Cui Zhifu Shan Han Zhou Li Wang Xinyuan Qiao Lijie Tang Xiaona Wang Yijing Li Source Type: research

Unraveling endophytic diversity in dioecious Siraitia grosvenorii: implications for mogroside production
Appl Microbiol Biotechnol. 2024 Mar 1;108(1):247. doi: 10.1007/s00253-024-13076-8.ABSTRACTHost and tissue-specificity of endophytes are important attributes that limit the endophyte application on multiple crops. Therefore, understanding the endophytic composition of the targeted crop is essential, especially for the dioecious plants where the male and female plants are different. Here, efforts were made to understand the endophytic bacterial composition of the dioecious Siraitia grosvenorii plant using 16S rRNA amplicon sequencing. The present study revealed the association of distinct endophytic bacterial communities wit...
Source: Applied Microbiology and Biotechnology - March 1, 2024 Category: Microbiology Authors: Anish Tamang Amanpreet Kaur Deepali Thakur Ankita Thakur Babit Kumar Thakur None Shivani Mohit Swarnkar Probir K Pal Vipin Hallan Shiv Shanker Pandey Source Type: research

A newly discovered glycosyltransferase gene UGT88A1 affects growth and polysaccharide synthesis of Grifola frondosa
In this study, the overexpression of UGT88A1 improved mycelial growth, increased polysaccharide production, and decreased cell wall pressure sensitivity. Biomass and polysaccharide production decreased in the silenced strain, and the pressure sensitivity of the cell wall increased. Overexpression and silencing of UGT88A1 both affected the monosaccharide composition and surface morphology of G. frondosa polysaccharides and influenced the antioxidant activity of polysaccharides from different strains. The messenger RNA expression of glucan synthase (GLS), UTP-glucose-1-phosphate uridylyltransferase (UGP), and UDP-xylose-4-ep...
Source: Applied Microbiology and Biotechnology - February 29, 2024 Category: Microbiology Authors: Jian Li Bao-Xin Wang Jie Zhang Na Han Shu-Ting Liu Wen-Ji Geng Shi-Ru Jia Yan-Ru Li Quan Gan Pei-Pei Han Source Type: research

The non-mevalonate pathway requires a delicate balance of intermediates to maximize terpene production
In this study, we integrated an additional copy of the entire MEP pathway into the E. coli genome for stable, marker-free terpene production. The genomically integrated strain produced more monoterpene geraniol than a plasmid-based system. The pathway genes' transcription was modulated using different promoters to produce geraniol as the reporter of the pathway flux. Pathway genes, including dxs, idi, and ispDF, expressed from a medium-strength promoter, led to the highest geraniol production. Quantifying the MEP pathway intermediates revealed that the highest geraniol producers had high levels of isopentenyl pyrophosphate...
Source: Applied Microbiology and Biotechnology - February 29, 2024 Category: Microbiology Authors: Indu Raghavan Rosheena Juman Zhen Q Wang Source Type: research

Analyzing microbial community and volatile compound profiles in the fermentation of cigar tobacco leaves
Appl Microbiol Biotechnol. 2024 Feb 29;108(1):243. doi: 10.1007/s00253-024-13043-3.ABSTRACTVariations in industrial fermentation techniques have a significant impact on the fermentation of cigar tobacco leaves (CTLs), consequently influencing the aromatic attributes of the resulting cigars. The entire fermentation process of CTLs can be categorized into three distinct phases: phase 1 (CTLs prior to moisture regain), phase 2 (CTLs post-moisture regain and pile fermentation), and phase 3 (CTLs after fermentation and drying). These phases were determined based on the dynamic changes in microbial community diversity. During ph...
Source: Applied Microbiology and Biotechnology - February 29, 2024 Category: Microbiology Authors: Mingzhu Zhang Dongfeng Guo Haiqing Wang Guanglong Wu Yaqi Shi Jinlong Zhou Eryong Zhao Tianfei Zheng Xingjiang Li Source Type: research

Secretory IgA reduced the ergosterol contents of Candida albicans to repress its hyphal growth and virulence
Appl Microbiol Biotechnol. 2024 Feb 29;108(1):244. doi: 10.1007/s00253-024-13063-z.ABSTRACTCandida albicans, one of the most prevalent conditional pathogenic fungi, can cause local superficial infections and lethal systemic infections, especially in the immunocompromised population. Secretory immunoglobulin A (sIgA) is an important immune protein regulating the pathogenicity of C. albicans. However, the actions and mechanisms that sIgA exerts directly against C. albicans are still unclear. Here, we investigated that sIgA directs against C. albicans hyphal growth and virulence to oral epithelial cells. Our results indicated...
Source: Applied Microbiology and Biotechnology - February 29, 2024 Category: Microbiology Authors: Jiannan Wang Jiawei Shen Ding Chen Binyou Liao Xi Chen Yawen Zong Yu Wei Yangyang Shi Yaqi Liu Lichen Gou Xuedong Zhou Lei Cheng Biao Ren Source Type: research

Low-temperature culture enhances production of flavivirus virus-like particles in mammalian cells
Appl Microbiol Biotechnol. 2024 Feb 28;108(1):242. doi: 10.1007/s00253-024-13064-y.ABSTRACTFlavivirus virus-like particles (VLPs) exhibit a striking structural resemblance to viral particles, making them highly adaptable for various applications, including vaccines and diagnostics. Consequently, increasing VLPs production is important and can be achieved by optimizing expression plasmids and cell culture conditions. While attempting to express genotype III (GIII) Japanese encephalitis virus (JEV) VLPs containing the G104H mutation in the envelope (E) protein, we failed to generate VLPs in COS-1 cells. However, VLPs product...
Source: Applied Microbiology and Biotechnology - February 28, 2024 Category: Microbiology Authors: Yi-Chin Fan Jo-Mei Chen Yi-Ying Chen Wei-Li Hsu Gwong-Jen Chang Shyan-Song Chiou Source Type: research