Approaches to produce and characterize recombinant protein VP1-2A of HAV for serological rapid test application
J Virol Methods. 2023 Nov 1:114839. doi: 10.1016/j.jviromet.2023.114839. Online ahead of print.ABSTRACTStudies reporting the expression of hepatitis A virus (HAV) structural proteins, specifically recombinant VP1-2A containing an immunogenic activity, use the Escherichia coli system. Recombinant HAV proteins may represent a source of less expensive antigens for application in different diagnostic platforms. However, the formation of insoluble aggregates is an obstacle to obtaining large amounts of HAV proteins in their native form. To overcome this obstacle, some approaches were applied in this study to improve purificatio...
Source: Journal of Virological Methods - November 3, 2023 Category: Virology Authors: Michel Vergne Felix Sucupira Ana Paula Corr êa Argondizzo Mariana Miguez Tardelli Garcia Anna Erika Vieira de Araujo Leila Botelho Rodrigues da Silva Marcelle Bral de Mello Christiane de F átima Silva Marques Danielle Regina de Almeida de Brito E Cunha Source Type: research
Approaches to produce and characterize recombinant protein VP1-2A of HAV for serological rapid test application
J Virol Methods. 2023 Nov 1:114839. doi: 10.1016/j.jviromet.2023.114839. Online ahead of print.ABSTRACTStudies reporting the expression of hepatitis A virus (HAV) structural proteins, specifically recombinant VP1-2A containing an immunogenic activity, use the Escherichia coli system. Recombinant HAV proteins may represent a source of less expensive antigens for application in different diagnostic platforms. However, the formation of insoluble aggregates is an obstacle to obtaining large amounts of HAV proteins in their native form. To overcome this obstacle, some approaches were applied in this study to improve purificatio...
Source: Journal of Virological Methods - November 3, 2023 Category: Virology Authors: Michel Vergne Felix Sucupira Ana Paula Corr êa Argondizzo Mariana Miguez Tardelli Garcia Anna Erika Vieira de Araujo Leila Botelho Rodrigues da Silva Marcelle Bral de Mello Christiane de F átima Silva Marques Danielle Regina de Almeida de Brito E Cunha Source Type: research
Approaches to produce and characterize recombinant protein VP1-2A of HAV for serological rapid test application
J Virol Methods. 2023 Nov 1:114839. doi: 10.1016/j.jviromet.2023.114839. Online ahead of print.ABSTRACTStudies reporting the expression of hepatitis A virus (HAV) structural proteins, specifically recombinant VP1-2A containing an immunogenic activity, use the Escherichia coli system. Recombinant HAV proteins may represent a source of less expensive antigens for application in different diagnostic platforms. However, the formation of insoluble aggregates is an obstacle to obtaining large amounts of HAV proteins in their native form. To overcome this obstacle, some approaches were applied in this study to improve purificatio...
Source: Journal of Virological Methods - November 3, 2023 Category: Virology Authors: Michel Vergne Felix Sucupira Ana Paula Corr êa Argondizzo Mariana Miguez Tardelli Garcia Anna Erika Vieira de Araujo Leila Botelho Rodrigues da Silva Marcelle Bral de Mello Christiane de F átima Silva Marques Danielle Regina de Almeida de Brito E Cunha Source Type: research
Vaccination Coverage by Age 24 Months Among Children Born in 2019 and 2020 - National Immunization Survey-Child, United States, 2020-2022
MMWR Morb Mortal Wkly Rep. 2023 Nov 3;72(44):1190-1196. doi: 10.15585/mmwr.mm7244a3.ABSTRACTNational Immunization Survey-Child data collected in 2022 were combined with data from previous years to assemble birth cohorts and assess coverage with routine vaccines by age 24 months by birth cohort. Overall, vaccination coverage was similar among children born during 2019-2020 compared with children born during 2017-2018, except that coverage with both the birth dose of hepatitis B vaccine and ≥1 dose of hepatitis A vaccine increased. Coverage was generally higher among non-Hispanic White (White) children (2-21 percentage poi...
Source: MMWR Morb Mortal Wkl... - November 2, 2023 Category: Epidemiology Authors: Holly A Hill David Yankey Laurie D Elam-Evans Michael Chen James A Singleton Source Type: research
Vaccination Coverage by Age 24 Months Among Children Born in 2019 and 2020 - National Immunization Survey-Child, United States, 2020-2022
MMWR Morb Mortal Wkly Rep. 2023 Nov 3;72(44):1190-1196. doi: 10.15585/mmwr.mm7244a3.ABSTRACTNational Immunization Survey-Child data collected in 2022 were combined with data from previous years to assemble birth cohorts and assess coverage with routine vaccines by age 24 months by birth cohort. Overall, vaccination coverage was similar among children born during 2019-2020 compared with children born during 2017-2018, except that coverage with both the birth dose of hepatitis B vaccine and ≥1 dose of hepatitis A vaccine increased. Coverage was generally higher among non-Hispanic White (White) children (2-21 percentage poi...
Source: MMWR Morb Mortal Wkl... - November 2, 2023 Category: Epidemiology Authors: Holly A Hill David Yankey Laurie D Elam-Evans Michael Chen James A Singleton Source Type: research
Vaccination Coverage by Age 24 Months Among Children Born in 2019 and 2020 - National Immunization Survey-Child, United States, 2020-2022
MMWR Morb Mortal Wkly Rep. 2023 Nov 3;72(44):1190-1196. doi: 10.15585/mmwr.mm7244a3.ABSTRACTNational Immunization Survey-Child data collected in 2022 were combined with data from previous years to assemble birth cohorts and assess coverage with routine vaccines by age 24 months by birth cohort. Overall, vaccination coverage was similar among children born during 2019-2020 compared with children born during 2017-2018, except that coverage with both the birth dose of hepatitis B vaccine and ≥1 dose of hepatitis A vaccine increased. Coverage was generally higher among non-Hispanic White (White) children (2-21 percentage poi...
Source: MMWR Morb Mortal Wkl... - November 2, 2023 Category: Epidemiology Authors: Holly A Hill David Yankey Laurie D Elam-Evans Michael Chen James A Singleton Source Type: research
Vaccination Coverage by Age 24 Months Among Children Born in 2019 and 2020 - National Immunization Survey-Child, United States, 2020-2022
MMWR Morb Mortal Wkly Rep. 2023 Nov 3;72(44):1190-1196. doi: 10.15585/mmwr.mm7244a3.ABSTRACTNational Immunization Survey-Child data collected in 2022 were combined with data from previous years to assemble birth cohorts and assess coverage with routine vaccines by age 24 months by birth cohort. Overall, vaccination coverage was similar among children born during 2019-2020 compared with children born during 2017-2018, except that coverage with both the birth dose of hepatitis B vaccine and ≥1 dose of hepatitis A vaccine increased. Coverage was generally higher among non-Hispanic White (White) children (2-21 percentage poi...
Source: MMWR Morb Mortal Wkl... - November 2, 2023 Category: Epidemiology Authors: Holly A Hill David Yankey Laurie D Elam-Evans Michael Chen James A Singleton Source Type: research
Vaccination Coverage by Age 24 Months Among Children Born in 2019 and 2020 - National Immunization Survey-Child, United States, 2020-2022
MMWR Morb Mortal Wkly Rep. 2023 Nov 3;72(44):1190-1196. doi: 10.15585/mmwr.mm7244a3.ABSTRACTNational Immunization Survey-Child data collected in 2022 were combined with data from previous years to assemble birth cohorts and assess coverage with routine vaccines by age 24 months by birth cohort. Overall, vaccination coverage was similar among children born during 2019-2020 compared with children born during 2017-2018, except that coverage with both the birth dose of hepatitis B vaccine and ≥1 dose of hepatitis A vaccine increased. Coverage was generally higher among non-Hispanic White (White) children (2-21 percentage poi...
Source: MMWR Morb Mortal Wkl... - November 2, 2023 Category: Epidemiology Authors: Holly A Hill David Yankey Laurie D Elam-Evans Michael Chen James A Singleton Source Type: research
Vaccination Coverage by Age 24 Months Among Children Born in 2019 and 2020 - National Immunization Survey-Child, United States, 2020-2022
MMWR Morb Mortal Wkly Rep. 2023 Nov 3;72(44):1190-1196. doi: 10.15585/mmwr.mm7244a3.ABSTRACTNational Immunization Survey-Child data collected in 2022 were combined with data from previous years to assemble birth cohorts and assess coverage with routine vaccines by age 24 months by birth cohort. Overall, vaccination coverage was similar among children born during 2019-2020 compared with children born during 2017-2018, except that coverage with both the birth dose of hepatitis B vaccine and ≥1 dose of hepatitis A vaccine increased. Coverage was generally higher among non-Hispanic White (White) children (2-21 percentage poi...
Source: MMWR Morb Mortal Wkl... - November 2, 2023 Category: Epidemiology Authors: Holly A Hill David Yankey Laurie D Elam-Evans Michael Chen James A Singleton Source Type: research
What ’s the problem represented to be? A critical analysis of problem representation in news media and public health communication during a hepatitis A outbreak in San Diego, California, USA
. (Source: Critical Public Health)
Source: Critical Public Health - October 31, 2023 Category: International Medicine & Public Health Authors: Jennifer K. FelnerAndrew StieberNichole McCuneElizabeth ReedJerel P. Calzoa Institute for Behavioral and Community Health, San Diego State University Research Foundation, San Diego, CA, USAb Division of Health Promotion and Behavioral Science, School of P Source Type: research
Study on association factors of intestinal infectious diseases based-Bayesian spatio-temporal model
CONCLUSIONS: This research systematically and quantitatively studied the effect of socioeconomic and meteorological factors on IIDs, which provided causal clues for future studies and guided government planning.PMID:37875791 | DOI:10.1186/s12879-023-08665-3 (Source: Cancer Control)
Source: Cancer Control - October 24, 2023 Category: Cancer & Oncology Authors: Yancen Zhan Hua Gu Xiuyang Li Source Type: research
Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release
Sci Adv. 2023 Oct 20;9(42):eadj4198. doi: 10.1126/sciadv.adj4198. Epub 2023 Oct 20.ABSTRACTVirus-induced changes in host lipid metabolism are an important but poorly understood aspect of viral pathogenesis. By combining nontargeted lipidomics analyses of infected cells and purified extracellular quasi-enveloped virions with high-throughput RNA sequencing and genetic depletion studies, we show that hepatitis A virus, an hepatotropic picornavirus, broadly manipulates the host cell lipid environment, enhancing synthesis of ceramides and other sphingolipids and transcriptionally activating acyl-coenzyme A synthetases and fatty...
Source: Adv Data - October 20, 2023 Category: Epidemiology Authors: Tomoyuki Shiota Zhucui Li Guan-Yuan Chen Kevin L McKnight Takayoshi Shirasaki Bryan Yonish Heyjeong Kim Ethan J Fritch Timothy P Sheahan Masamichi Muramatsu Maryna Kapustina Craig E Cameron You Li Qibin Zhang Stanley M Lemon Source Type: research
Preventable Deaths During Widespread Community Hepatitis A Outbreaks - United States, 2016-2022
MMWR Morb Mortal Wkly Rep. 2023 Oct 20;72(42):1128-1133. doi: 10.15585/mmwr.mm7242a1.ABSTRACTHepatitis A is acquired through the fecal-oral route and is preventable by a safe and effective vaccine. Although hepatitis A is generally mild and self-limited, serious complications, including death, can occur. Since 2016, widespread hepatitis A outbreaks have been reported in 37 U.S. states, primarily among persons who use drugs and those experiencing homelessness. Nearly twice as many hepatitis A-related deaths were reported during 2016-2022 compared with 2009-2015. CDC analyzed data from 27 hepatitis A outbreak-affected states...
Source: MMWR Morb Mortal Wkl... - October 19, 2023 Category: Epidemiology Authors: Megan G Hofmeister Neil Gupta Hepatitis A Mortality Investigators Source Type: research
Preventable Deaths During Widespread Community Hepatitis A Outbreaks - United States, 2016-2022
MMWR Morb Mortal Wkly Rep. 2023 Oct 20;72(42):1128-1133. doi: 10.15585/mmwr.mm7242a1.ABSTRACTHepatitis A is acquired through the fecal-oral route and is preventable by a safe and effective vaccine. Although hepatitis A is generally mild and self-limited, serious complications, including death, can occur. Since 2016, widespread hepatitis A outbreaks have been reported in 37 U.S. states, primarily among persons who use drugs and those experiencing homelessness. Nearly twice as many hepatitis A-related deaths were reported during 2016-2022 compared with 2009-2015. CDC analyzed data from 27 hepatitis A outbreak-affected states...
Source: MMWR Morb Mortal Wkl... - October 19, 2023 Category: Epidemiology Authors: Megan G Hofmeister Neil Gupta Hepatitis A Mortality Investigators Source Type: research
