A novel pyrosequencing assay for the detection of neuraminidase inhibitor resistance-conferring mutations among clinical isolates of avian H7N9 influenza virus.
In this study, we provide a rapid and reliable method for the characterization of NAI resistance in H7N9 viruses.
PMID: 24211668 [PubMed - as supplied by publisher] (Source: Virus Research)
Source: Virus Research - November 5, 2013 Category: Virology Authors: Qi Y, Fan H, Qi X, Zhu Z, Guo X, Chen Y, Ge Y, Zhao K, Wu T, Li Y, Shan Y, Zhou M, Shi Z, Wang H, Cui L Tags: Virus Res Source Type: research
Computational and experimental analysis of drug binding to the Influenza M2 channel.
This article is part of a Special Issue entitled: Viral Membrane Proteins - Channels for Cellular Networking.
PMID: 24016551 [PubMed - as supplied by publisher] (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - September 6, 2013 Category: Biochemistry Authors: Alhadeff R, Assa D, Astrahan P, Krugliak M, Arkin IT Tags: Biochim Biophys Acta Source Type: research
Structure‐guided design affirms inhibitors of hepatitis C virus p7 as a viable class of antivirals targeting virion release
Conclusions: This proof‐of‐principle that structure‐guided design can lead to drug‐like molecules affirms p7 as a much needed new target in the burgeoning era of HCV DAA. (Hepatology 2013;) (Source: Hepatology)
Source: Hepatology - August 12, 2013 Category: Internal Medicine Authors: Toshana L. Foster, Gary S. Thompson, Arnout P. Kalverda, Jayakanth Kankanala, Matthew Bentham, Laura F. Wetherill, Joseph Thompson, Amy M. Barker, Dean Clarke, Marko Noerenberg, Arwen R. Pearson, David J. Rowlands, Steven W. Homans, Mark Harris, Richard F Tags: Viral Hepatitis Source Type: research
[Comment] Oseltamivir resistance during treatment of H7N9 infection
Detection of an Arg292Lys mutation (N2 numbering) in the virus neuraminidase (NA) gene within 2 days of initiating oseltamivir treatment, in the first reported human infection by avian A(H7N9) influenza virus, raised concern about emergence of resistance during treatment with neuraminidase inhibitors. This is important because a Ser31Asn resistance mutation in the M2 protein of A(H7N9) viruses, also present in A(H1N1)pdm09 and seasonal A(H3N2) viruses, abrogates effectiveness of available M2 inhibitors (amantadine and rimantadine). (Source: LANCET)
Source: LANCET - June 29, 2013 Category: Journals (General) Authors: Alan J Hay, Frederick G Hayden Tags: Comment Source Type: research
Unusual architecture of the p7 channel from hepatitis C virus
amp; James J. Chou
The hepatitis C virus (HCV) has developed a small membrane protein, p7, which remarkably can self-assemble into a large channel complex that selectively conducts cations. We wanted to examine the structural solution that the viroporin adopts in order to achieve selective cation conduction, because p7 has no homology with any of the known prokaryotic or eukaryotic channel proteins. The activity of p7 can be inhibited by amantadine and rimantadine, which are potent blockers of the influenza M2 channel and licensed drugs against influenza infections. The adamantane derivatives have been used in HCV...
Source: Nature - June 5, 2013 Category: Research Authors: Bo OuYangShiqi XieMarcelo J. BerardiXinhao ZhaoJyoti DevWenjing YuBing SunJames J. Chou Tags: Letter Source Type: research
Unusual architecture of the p7 channel from hepatitis C virus
Nature advance online publication 05 June 2013. doi:10.1038/nature12283
Authors: Bo OuYang, Shiqi Xie, Marcelo J. Berardi, Xinhao Zhao, Jyoti Dev, Wenjing Yu, Bing Sun & James J. Chou
The hepatitis C virus (HCV) has developed a small membrane protein, p7, which remarkably can self-assemble into a large channel complex that selectively conducts cations. We wanted to examine the structural solution that the viroporin adopts in order to achieve selective cation conduction, because p7 has no homology with any of the known prokaryotic or eukaryotic channel proteins. The activity of p7 can be inhibited by amantadine and rim...
Source: Nature AOP - June 5, 2013 Category: Research Authors: Bo OuYangShiqi XieMarcelo J. BerardiXinhao ZhaoJyoti DevWenjing YuBing SunJames J. Chou Tags: Letter Source Type: research
Guidance for clinical and public health laboratories testing for influenza virus antiviral drug susceptibility in Europe
Abstract: Two classes of antiviral drugs are licensed in Europe for treatment and prophylaxis of influenza; the M2 ion-channel blockers amantadine and rimantadine acting against type A influenza viruses only and the neuraminidase enzyme inhibitors zanamivir and oseltamivir acting against type A and type B influenza viruses.This guidance document was developed for but not limited to the European Union (EU) and other European Economic Area (EEA) countries on how and when to test for influenza virus antiviral drug susceptibility. It is aimed at clinical and influenza surveillance laboratories carrying out antiviral drug susce...
Source: Journal of Clinical Virology - February 4, 2013 Category: Virology Authors: Francisco Pozo, Bruno Lina, Helena Rebelo de Andrade, Vincent Enouf, Athanasios Kossyvakis, Eeva Broberg, Rod Daniels, Angie Lackenby, Adam Meijer Tags: Laboratory practice guidelines Source Type: research
Structure, mechanism, and inhibition of A/M2-S31N [Chemistry]
The influenza A virus M2 proton channel (A/M2) is the target of the antiviral drugs amantadine and rimantadine, whose use has been discontinued due to widespread drug resistance. Among the handful of drug-resistant mutants, S31N is found in more than 95% of the currently circulating viruses and shows greatly decreased... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - January 22, 2013 Category: Science Authors: Wang, J., Wu, Y., Ma, C., Fiorin, G., , Pinto, L. H., Lamb, R. A., Klein, M. L., DeGrado, W. F. Tags: Biological Sciences Source Type: research
Antiviral Activity of the Plant Extracts from Thuja orientalis, Aster spathulifolius, and Pinus thunbergii Against Influenza Virus A/PR/8/34.
In this study, we examined the antiviral efficacy of the plant extracts against the influenza A/PR/8/34 infection. In vitro, the antiviral activities of the plant extracts were investigated using the cell-based screening. Three plant extracts, Thuja orientalis, Aster spathulifolius, and Pinus thunbergii, were shown to induce a high cell viability rate after the infection with the influenza A/PR/8/34 virus. The antiviral activity of the plant extracts also increased as a function of the concentration of the extracts and these extracts significantly reduced the visible cytopathic effect caused by virus infections. Furthermor...
Source: Journal of Microbiology and Biotechnology - January 1, 2013 Category: Biotechnology Authors: Won JN, Lee SY, Song DS, Poo H Tags: J Microbiol Biotechnol Source Type: research
