l-carnitine enhances the kinematics and protects the sperm membranes of chilled and frozen-thawed Peruvian Paso horse spermatozoa
In conclusion, supplementation ofl-carnitine to skimmed milk-based extenders enhanced kinematic variables and protected the membrane integrity in chilled and frozen-thawed Peruvian Paso horse spermatozoa.PMID:38460835 | DOI:10.1016/j.cryobiol.2024.104884 (Source: Cryobiology)
Source: Cryobiology - March 9, 2024 Category: Biology Authors: Paula Palacios Gabriela Pel áez Manuel Soria Silvana M éndez Luis Galarza- Álvarez Jes ús Dorado Juli án Santiago-Moreno Diego A Galarza Source Type: research

l-carnitine enhances the kinematics and protects the sperm membranes of chilled and frozen-thawed Peruvian Paso horse spermatozoa
In conclusion, supplementation ofl-carnitine to skimmed milk-based extenders enhanced kinematic variables and protected the membrane integrity in chilled and frozen-thawed Peruvian Paso horse spermatozoa.PMID:38460835 | DOI:10.1016/j.cryobiol.2024.104884 (Source: Cryobiology)
Source: Cryobiology - March 9, 2024 Category: Biology Authors: Paula Palacios Gabriela Pel áez Manuel Soria Silvana M éndez Luis Galarza- Álvarez Jes ús Dorado Juli án Santiago-Moreno Diego A Galarza Source Type: research

l-carnitine enhances the kinematics and protects the sperm membranes of chilled and frozen-thawed Peruvian Paso horse spermatozoa
In conclusion, supplementation of l-carnitine to skimmed milk-based extenders enhanced kinematic variables and protected the membrane integrity in chilled and frozen-thawed Peruvian Paso horse spermatozoa.PMID:38460835 | DOI:10.1016/j.cryobiol.2024.104884 (Source: Cryobiology)
Source: Cryobiology - March 9, 2024 Category: Biology Authors: Paula Palacios Gabriela Pel áez Manuel Soria Silvana M éndez Luis Galarza- Álvarez Jes ús Dorado Juli án Santiago-Moreno Diego A Galarza Source Type: research

l-carnitine enhances the kinematics and protects the sperm membranes of chilled and frozen-thawed Peruvian Paso horse spermatozoa
In conclusion, supplementation of l-carnitine to skimmed milk-based extenders enhanced kinematic variables and protected the membrane integrity in chilled and frozen-thawed Peruvian Paso horse spermatozoa.PMID:38460835 | DOI:10.1016/j.cryobiol.2024.104884 (Source: Cryobiology)
Source: Cryobiology - March 9, 2024 Category: Biology Authors: Paula Palacios Gabriela Pel áez Manuel Soria Silvana M éndez Luis Galarza- Álvarez Jes ús Dorado Juli án Santiago-Moreno Diego A Galarza Source Type: research

l-carnitine enhances the kinematics and protects the sperm membranes of chilled and frozen-thawed Peruvian Paso horse spermatozoa
In conclusion, supplementation of l-carnitine to skimmed milk-based extenders enhanced kinematic variables and protected the membrane integrity in chilled and frozen-thawed Peruvian Paso horse spermatozoa.PMID:38460835 | DOI:10.1016/j.cryobiol.2024.104884 (Source: Cryobiology)
Source: Cryobiology - March 9, 2024 Category: Biology Authors: Paula Palacios Gabriela Pel áez Manuel Soria Silvana M éndez Luis Galarza- Álvarez Jes ús Dorado Juli án Santiago-Moreno Diego A Galarza Source Type: research

l-carnitine enhances the kinematics and protects the sperm membranes of chilled and frozen-thawed Peruvian Paso horse spermatozoa
In conclusion, supplementation of l-carnitine to skimmed milk-based extenders enhanced kinematic variables and protected the membrane integrity in chilled and frozen-thawed Peruvian Paso horse spermatozoa.PMID:38460835 | DOI:10.1016/j.cryobiol.2024.104884 (Source: Cryobiology)
Source: Cryobiology - March 9, 2024 Category: Biology Authors: Paula Palacios Gabriela Pel áez Manuel Soria Silvana M éndez Luis Galarza- Álvarez Jes ús Dorado Juli án Santiago-Moreno Diego A Galarza Source Type: research

Effect of Trehalose Supplementation in Egg-Yolk-Free Extender on Conventional Parameters and Gene Expression Related to Reactive Oxygen Species, Apoptosis, and Motility of Frozen Dog Spermatozoa
Biopreserv Biobank. 2024 Mar 7. doi: 10.1089/bio.2023.0082. Online ahead of print.ABSTRACTThe present study was conducted to evaluate the effects of trehalose supplementation in egg-yolk (EY)-free tris extender on dog spermatozoa. Pooled spermatozoa were diluted with extender 1 (EY-free tris extender supplemented with 0, 10, 15, 20, or 30 mM trehalose) and cooled (2 × 108 sperm/mL) for 1 hour at 4°C. After that, extender 2 (extender 1 containing 1 M glycerol) was added (v:v) to the diluted sperm, loaded in 0.5-mL straws (1 × 108 sperm/mL), and incubated at 4°C for 30 minutes. The sperm straws were frozen over liquid ni...
Source: Biopreservation and Biobanking - March 7, 2024 Category: Biomedical Science Authors: Saddah Ibrahim Sangmin Shin Nabeel Abdelbagi Hamad Talha Yubyeol Jeon Il-Jeoung Yu Source Type: research

Diagnostic advancements: Isolating Mycobacterium avium ssp. paratuberculosis and unveiling its molecular identity with nested-PCR
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):18-23. doi: 10.14715/cmb/2024.70.2.3.ABSTRACTMycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of paratuberculosis, which is currently prevalent in many parts of Iran and produces severe economic loss. It is hence necessary to identify and isolate the animals infected with this bacterium, so this research aimed to isolate MAP from milk and fecal samples of ELISA-positive animals and determine the molecular identity of isolates. After performing ELISA on 3,700 bovine blood samples, 115 samples of milk and feces were taken from ELISA-positive cattle...
Source: Mol Biol Cell - March 2, 2024 Category: Molecular Biology Authors: Faranak Nouri Alireza Shahrjerdi Hossein Ali Zarnegarpour Nader Mosvari Source Type: research

Diagnostic advancements: Isolating Mycobacterium avium ssp. paratuberculosis and unveiling its molecular identity with nested-PCR
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):18-23. doi: 10.14715/cmb/2024.70.2.3.ABSTRACTMycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of paratuberculosis, which is currently prevalent in many parts of Iran and produces severe economic loss. It is hence necessary to identify and isolate the animals infected with this bacterium, so this research aimed to isolate MAP from milk and fecal samples of ELISA-positive animals and determine the molecular identity of isolates. After performing ELISA on 3,700 bovine blood samples, 115 samples of milk and feces were taken from ELISA-positive cattle...
Source: Cellular and Molecular Biology - March 2, 2024 Category: Molecular Biology Authors: Faranak Nouri Alireza Shahrjerdi Hossein Ali Zarnegarpour Nader Mosvari Source Type: research

Diagnostic advancements: Isolating Mycobacterium avium ssp. paratuberculosis and unveiling its molecular identity with nested-PCR
Cell Mol Biol (Noisy-le-grand). 2024 Feb 29;70(2):18-23. doi: 10.14715/cmb/2024.70.2.3.ABSTRACTMycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of paratuberculosis, which is currently prevalent in many parts of Iran and produces severe economic loss. It is hence necessary to identify and isolate the animals infected with this bacterium, so this research aimed to isolate MAP from milk and fecal samples of ELISA-positive animals and determine the molecular identity of isolates. After performing ELISA on 3,700 bovine blood samples, 115 samples of milk and feces were taken from ELISA-positive cattle...
Source: Mol Biol Cell - March 2, 2024 Category: Molecular Biology Authors: Faranak Nouri Alireza Shahrjerdi Hossein Ali Zarnegarpour Nader Mosvari Source Type: research

FcRY is a key molecule controlling maternal blood IgY transfer to yolks during egg development in avian species
Maternal immunoglobulin transfer plays a key role in conferring passive immunity to neonates. Maternal blood immunoglobulin Y (IgY) in avian species is transported to newly-hatched chicks in two steps: 1) IgY is transported from the maternal circulation to the yolk of maturing oocytes, 2) the IgY deposited in yolk is transported to the circulation of the embryo via the yolk sac membrane. An IgY-Fc receptor, FcRY, is involved in the second step, but the mechanism of the first step is still unclear. We determined whether FcRY was also the basis for maternal blood IgY transfer to the yolk in the first step during egg developm...
Source: Frontiers in Immunology - February 29, 2024 Category: Allergy & Immunology Source Type: research

Dietary Chinese herbal formula supplementation improves yolk fatty acid profile in aged laying hens
Vet Q. 2024 Dec;44(1):1-11. doi: 10.1080/01652176.2024.2319828. Epub 2024 Feb 25.ABSTRACTChinese herbal formula (CHF) has the potential to improve the performance of aged laying hens through integrated regulation of various physiological functions. The present study aimed to investigate the effects of dietary CHF supplementation on the yolk fatty acid profile in aged laying hens. A total of 144 healthy 307-day-old Xinyang black-feather laying hens were randomly allocated into two groups: a control group (CON, fed a basal diet) and a CHF group (fed a basal diet supplemented with 1% CHF; contained 0.30% Leonurus japonicus Ho...
Source: Veterinary Quarterly - February 26, 2024 Category: Veterinary Research Authors: Zhihua Li Chengwen Meng Md Abul Kalam Azad Wencao Lin Jue Gui Yadong Cui Wei Lan Qinghua He Xiangfeng Kong Source Type: research

Dietary Chinese herbal formula supplementation improves yolk fatty acid profile in aged laying hens
Vet Q. 2024 Dec;44(1):1-11. doi: 10.1080/01652176.2024.2319828. Epub 2024 Feb 25.ABSTRACTChinese herbal formula (CHF) has the potential to improve the performance of aged laying hens through integrated regulation of various physiological functions. The present study aimed to investigate the effects of dietary CHF supplementation on the yolk fatty acid profile in aged laying hens. A total of 144 healthy 307-day-old Xinyang black-feather laying hens were randomly allocated into two groups: a control group (CON, fed a basal diet) and a CHF group (fed a basal diet supplemented with 1% CHF; contained 0.30% Leonurus japonicus Ho...
Source: Veterinary Quarterly - February 26, 2024 Category: Veterinary Research Authors: Zhihua Li Chengwen Meng Md Abul Kalam Azad Wencao Lin Jue Gui Yadong Cui Wei Lan Qinghua He Xiangfeng Kong Source Type: research