Simplifying stable CHO cell line generation with high probability of monoclonality by using microfluidic dispensing as an alternative to fluorescence activated cell sorting
AbstractSingle cell cloning is a critical step for cell line development (CLD) for therapeutic protein production, with proof of monoclonality being compulsorily sought in regulatory filings. Among the different single cell deposition technologies, we found that fluorescence activated cell sorting (FACS) offers high probability of monoclonality and can allow selective enrichment of the producer cells. However, FACS instruments are expensive and resource-intensive, have a large footprint, require highly skilled operators and take hours for setup, thereby complicating the cell line generation process. With the aim of finding...
Source: Biotechnology Progress - March 11, 2024 Category: Biotechnology Authors: Lina Chakrabarti,
James Savery,
John Patrick Mpindi,
Judith Klover,
Lina Li,
Jie Zhu Tags: RESEARCH ARTICLE Source Type: research
Is Economic Evaluation and Care Commissioning Focused on Achieving the Same Outcomes? Resource-Allocation Considerations and Challenges Using England as a Case Study
We describe considerations and challenges as to the practical role of relevant outcomes for evaluation and commissioning, seeking to bridge a gap between economic evaluation evidence and care commissioning. We describe conceptual (e.g., what are ‘relevant’ outcomes) alongside practical considerations (e.g., quantifying and using relevant endpoint or surrogate outcomes) and pertinent issues when linking outcomes to commissioning-based payment mechanisms, using England as a case study. Economic evaluation often focuses on a single endpoin t health-focused maximand, e.g., quality-adjusted life-years (QALYs), whereas commi...
Source: Applied Health Economics and Health Policy - March 11, 2024 Category: Health Management Source Type: research
Coral restoration can drive rapid reef carbonate budget recovery
Curr Biol. 2024 Feb 19:S0960-9822(24)00151-9. doi: 10.1016/j.cub.2024.02.009. Online ahead of print.ABSTRACTRestoration is increasingly seen as a necessary tool to reverse ecological decline across terrestrial and marine ecosystems.1,2 Considering the unprecedented loss of coral cover and associated reef ecosystem services, active coral restoration is gaining traction in local management strategies and has recently seen major increases in scale. However, the extent to which coral restoration may restore key reef functions is poorly understood.3,4 Carbonate budgets, defined as the balance between calcium carbonate productio...
Source: Current Biology - March 9, 2024 Category: Biology Authors: Ines D Lange Tries B Razak Chris T Perry Permas B Maulana Mochyudho E Prasetya None Irwan Timothy Ac Lamont Source Type: research
Coral restoration can drive rapid reef carbonate budget recovery
Curr Biol. 2024 Feb 19:S0960-9822(24)00151-9. doi: 10.1016/j.cub.2024.02.009. Online ahead of print.ABSTRACTRestoration is increasingly seen as a necessary tool to reverse ecological decline across terrestrial and marine ecosystems.1,2 Considering the unprecedented loss of coral cover and associated reef ecosystem services, active coral restoration is gaining traction in local management strategies and has recently seen major increases in scale. However, the extent to which coral restoration may restore key reef functions is poorly understood.3,4 Carbonate budgets, defined as the balance between calcium carbonate productio...
Source: Current Biology - March 9, 2024 Category: Biology Authors: Ines D Lange Tries B Razak Chris T Perry Permas B Maulana Mochyudho E Prasetya None Irwan Timothy Ac Lamont Source Type: research
Coral restoration can drive rapid reef carbonate budget recovery
Curr Biol. 2024 Feb 19:S0960-9822(24)00151-9. doi: 10.1016/j.cub.2024.02.009. Online ahead of print.ABSTRACTRestoration is increasingly seen as a necessary tool to reverse ecological decline across terrestrial and marine ecosystems.1,2 Considering the unprecedented loss of coral cover and associated reef ecosystem services, active coral restoration is gaining traction in local management strategies and has recently seen major increases in scale. However, the extent to which coral restoration may restore key reef functions is poorly understood.3,4 Carbonate budgets, defined as the balance between calcium carbonate productio...
Source: Current Biology - March 9, 2024 Category: Biology Authors: Ines D Lange Tries B Razak Chris T Perry Permas B Maulana Mochyudho E Prasetya None Irwan Timothy Ac Lamont Source Type: research
The emergence of the Medical University of Vienna 20 years ago
Wien Klin Wochenschr. 2024 Mar 8. doi: 10.1007/s00508-024-02334-4. Online ahead of print.ABSTRACTAt the beginning of the 2000s the Austrian public universities were characterized by staffing rigidities, little competitive research, outdated study curricula and free access to all fields of study, the latter combined with high dropout rates and long study durations. As a countermeasure the universities were granted full legal capacity. For new employees the status of civil servants was herewith cancelled and, being now subject to the Employment Act, tenured employments for anyone who wanted to stay at the university were no ...
Source: Wiener Klinische Wochenschrift - March 8, 2024 Category: General Medicine Authors: Wolfgang Sch ütz Markus Grimm Source Type: research
Assessing the Negative Binomial-Lindley model for crash hotspot identification: Insights from Monte Carlo simulation analysis
Accid Anal Prev. 2024 Mar 7;199:107478. doi: 10.1016/j.aap.2024.107478. Online ahead of print.ABSTRACTIdentifying hazardous crash sites (or hotspots) is a crucial step in highway safety management. The Negative Binomial (NB) model is the most common model used in safety analyses and evaluations - including hotspot identification. The NB model, however, is not without limitations. In fact, this model does not perform well when data are highly dispersed, include excess zero observations, or have a long tail. Recently, the Negative Binomial-Lindley (NB-L) model has been proposed as an alternative to the NB. The NB-L model ove...
Source: Accident; Analysis and Prevention. - March 8, 2024 Category: Accident Prevention Authors: Jhan Kevin Gil-Marin Mohammadali Shirazi John N Ivan Source Type: research
The emergence of the Medical University of Vienna 20 years ago
Wien Klin Wochenschr. 2024 Mar 8. doi: 10.1007/s00508-024-02334-4. Online ahead of print.ABSTRACTAt the beginning of the 2000s the Austrian public universities were characterized by staffing rigidities, little competitive research, outdated study curricula and free access to all fields of study, the latter combined with high dropout rates and long study durations. As a countermeasure the universities were granted full legal capacity. For new employees the status of civil servants was herewith cancelled and, being now subject to the Employment Act, tenured employments for anyone who wanted to stay at the university were no ...
Source: Wiener Klinische Wochenschrift - March 8, 2024 Category: General Medicine Authors: Wolfgang Sch ütz Markus Grimm Source Type: research
Assessing the Negative Binomial-Lindley model for crash hotspot identification: Insights from Monte Carlo simulation analysis
Accid Anal Prev. 2024 Mar 7;199:107478. doi: 10.1016/j.aap.2024.107478. Online ahead of print.ABSTRACTIdentifying hazardous crash sites (or hotspots) is a crucial step in highway safety management. The Negative Binomial (NB) model is the most common model used in safety analyses and evaluations - including hotspot identification. The NB model, however, is not without limitations. In fact, this model does not perform well when data are highly dispersed, include excess zero observations, or have a long tail. Recently, the Negative Binomial-Lindley (NB-L) model has been proposed as an alternative to the NB. The NB-L model ove...
Source: Accident; Analysis and Prevention. - March 8, 2024 Category: Accident Prevention Authors: Jhan Kevin Gil-Marin Mohammadali Shirazi John N Ivan Source Type: research
The emergence of the Medical University of Vienna 20 years ago
Wien Klin Wochenschr. 2024 Mar 8. doi: 10.1007/s00508-024-02334-4. Online ahead of print.ABSTRACTAt the beginning of the 2000s the Austrian public universities were characterized by staffing rigidities, little competitive research, outdated study curricula and free access to all fields of study, the latter combined with high dropout rates and long study durations. As a countermeasure the universities were granted full legal capacity. For new employees the status of civil servants was herewith cancelled and, being now subject to the Employment Act, tenured employments for anyone who wanted to stay at the university were no ...
Source: Wiener Klinische Wochenschrift - March 8, 2024 Category: General Medicine Authors: Wolfgang Sch ütz Markus Grimm Source Type: research
The emergence of the Medical University of Vienna 20 years ago
Wien Klin Wochenschr. 2024 Mar 8. doi: 10.1007/s00508-024-02334-4. Online ahead of print.ABSTRACTAt the beginning of the 2000s the Austrian public universities were characterized by staffing rigidities, little competitive research, outdated study curricula and free access to all fields of study, the latter combined with high dropout rates and long study durations. As a countermeasure the universities were granted full legal capacity. For new employees the status of civil servants was herewith cancelled and, being now subject to the Employment Act, tenured employments for anyone who wanted to stay at the university were no ...
Source: Wiener Klinische Wochenschrift - March 8, 2024 Category: General Medicine Authors: Wolfgang Sch ütz Markus Grimm Source Type: research
News at a glance: Methane-hunting satellite, Elsevier ’s earnings, and protecting Kinsey
ENVIRONMENT
White House wants to weigh ecosystem benefits
President Joe Biden’s administration published guidance last week for U.S. federal agencies to weigh benefits to human health and welfare provided by natural resources when considering whether to undertake or approve actions that could harm them. These “ecosystem benefits” include clean water and air and intangible perks such as spiritual connections to nature. Until now, federal rules have required agencies to analyze costs and benefits when building roads or issuing permits for logging or mining, for example, but did not explicitly men...
Source: Science of Aging Knowledge Environment - March 7, 2024 Category: Geriatrics Source Type: research
Landscaping analysis of immunization progress and program structures in selected middle income Southeast Asian countries
This study examined the performance and structures of national immunization program in five middle-income Southeast Asian countries - Malaysia, Thailand, Philippines, Viet Nam, and Myanmar, and analyzed how the different structures relate to the difference in program performance to identify effective strategies in the study countries that facilitated good immunization performance. Data were derived from published literature, and WHO/UNICEF/Gavi databases, with 2010 as the baseline year. UMICs Malaysia and Thailand maintained ≥90 % coverage from 2010 to 2020 and even during the COVID-19 pandemic in 2021. LMICs Viet Nam an...
Source: Vaccine - March 6, 2024 Category: Allergy & Immunology Authors: Jessabelle E Basa Ralf Clemens Sue Ann Costa Clemens Martin Nicholson Source Type: research
Landscaping analysis of immunization progress and program structures in selected middle income Southeast Asian countries
This study examined the performance and structures of national immunization program in five middle-income Southeast Asian countries - Malaysia, Thailand, Philippines, Viet Nam, and Myanmar, and analyzed how the different structures relate to the difference in program performance to identify effective strategies in the study countries that facilitated good immunization performance. Data were derived from published literature, and WHO/UNICEF/Gavi databases, with 2010 as the baseline year. UMICs Malaysia and Thailand maintained ≥90 % coverage from 2010 to 2020 and even during the COVID-19 pandemic in 2021. LMICs Viet Nam an...
Source: Vaccine - March 6, 2024 Category: Allergy & Immunology Authors: Jessabelle E Basa Ralf Clemens Sue Ann Costa Clemens Martin Nicholson Source Type: research
Determining the cost and cost-effectiveness of childhood cancer treatment in Haiti
Ecancermedicalscience. 2024 Feb 28;18:1675. doi: 10.3332/ecancer.2024.1675. eCollection 2024.ABSTRACTHaiti is a low-income country with one of the lowest human development index rankings in the world. Its childhood cancer services are provided by a single hospital with the only dedicated paediatric oncology department in the country. Our objective was to assess the cost and cost-effectiveness of all types of childhood cancer in Haiti to help prioritise investments and to support national cancer control planning. All costing data were collected from the year 2017 or 2018 hospital records. Costs were classified into 11 cost ...
Source: Ecancermedicalscience - March 5, 2024 Category: Cancer & Oncology Authors: Nancy S Bolous Peter Mercredi Miguel Bonilla Paola Friedrich Nickhill Bhakta Monika L Metzger Pascale Y Gassant Source Type: research
