< i > Aedes aegypti dyspepsia < /i > encodes a novel member of the SLC16 family of transporters and is critical for reproductive fitness

In this study, we performed an RNAseq-based screen in anAe.aegypti cell line cultured under iron-deficient and iron-excess conditions. A subset of differentially expressed genes were analyzed via a cytosolic iron-sensitive dual-luciferase reporter assay with several gene candidates potentially involved in iron transport.In vivo gene silencing resulted in significant reduction of fecundity (egg number) and fertility (hatch rate) for one gene, termeddyspepsia. Silencing ofdyspepsia reduced the induction of ferritin expression in the midgut and also resulted in delayed/impaired excretion and digestion. Further characterization of this gene, including a more direct confirmation of its substrate (iron or otherwise), could inform vector control strategies as well as to contribute to the field of metal biology.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research