Alteration of protein profile in cerebral cortex of rats exposed to Bisphenol A: A proteomics study.

Alteration of protein profile in cerebral cortex of rats exposed to Bisphenol A: A proteomics study. Neurotoxicology. 2020 Feb 01;: Authors: Tavakkoli A, Abnous K, Hassani FV, Hosseinzadeh H, Birner-Gruenberger R, Mehri S Abstract Bisphenol A (BPA) is one of the most widely used chemicals in plastic industry, which enters the human body through occupational and food contact. We studied the protein changes in rat cerebral cortex to evaluate the neurotoxicity of BPA. Twenty-four adult male rats were randomly selected and divided into four groups and each group respectively received 0, 0.5, 5 and 50 mg/kg of BPA for 4 weeks orally. To determine the oxidative status, reduced glutathione content and the level of malondialdehyde were measured in brain cortical tissue. The proteins of each sample extracted and separated on a two-dimensional acrylamide gel electrophoresis. From the obtained protein map, the 10 most altered protein spots were used for mass spectroscopy analysis. The lipid peroxidation in both doses of 0.5 and 5 mg/kg was significantly higher than the control group, but the glutathione content had no significant difference between the groups. Based on the results of the MS data analysis by the MASCOT database search engine, 10 proteins with altered intensity were identified as pyruvate kinase, alpha-enolase, aconitate hydratase, creatine kinase B-type, phosphatidylethanolamine-binding protein 1, 14-3-3 protein eta, guanine...
Source: Neurotoxicology - Category: Neurology Authors: Tags: Neurotoxicology Source Type: research