Comparative Proteomic Profiling of Methicillin ‐Susceptible and Resistant Staphylococcus aureus

This study provides insights into the virulence, pathogenicity, and antimicrobial resistance determinants of methicillin ‐susceptible and methicillin‐resistantS. aureus (MSSA; MRSA) recovered from non ‐healthcare environments. Three environmental MSSA and three environmental MRSA are selected for proteomic profiling using isobaric tag for relative and absolute quantitation tandem mass spectrometry (iTRAQ MS/MS). Gene Ontology annotation and Kyoto Encyclopedia of Genes and Genomes pathway annota tion are applied to interpret the functions of the proteins detected. 792 proteins are identified in MSSA and MRSA. Comparative analysis of MRSA and MSSA reveals that 8 of out 792 proteins are upregulated and 156 are downregulated. Proteins that have differences in abundance are predominantly involv ed in catalytic and binding activity. Among 164 differently abundant proteins, 29 are involved in pathogenesis, antimicrobial resistance, stress response, mismatch repair, and cell wall synthesis. Twenty‐two proteins associated with pathogenicity including SPA, SBI, CLFA, and DLT are upregulated i n MRSA. Moreover, the upregulated pathogenic protein ENTC2 in MSSA is determined to be a super antigen, potentially capable of triggering toxic shock syndrome in the host. Enhanced pathogenicity, antimicrobial resistance, and stress response are observed in MRSA compared to MSSA.
Source: Proteomics - Category: Biochemistry Authors: Tags: Dataset Brief Source Type: research

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Source: Journal of Ophthalmic Inflammation and Infection - Category: Opthalmology Source Type: research
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Source: Redox Report : communications in free radical research - Category: Biochemistry Authors: Tags: Redox Rep Source Type: research
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Source: Meta Gene - Category: Genetics & Stem Cells Source Type: research
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Source: European Review for Medical and Pharmacological Sciences - Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
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Source: European Review for Medical and Pharmacological Sciences - Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
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Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
ConclusionsEnhanced pathogenicity, antimicrobial resistance and stress response were observed in MRSA compared to MSSA.This article is protected by copyright. All rights reserved
Source: Proteomics - Category: Biochemistry Authors: Tags: Dataset Brief Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research
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