Molecules, Vol. 24, Pages 3665: Assessing the Utility of Multiplexed Liquid Chromatography-Mass Spectrometry for Gluten Detection in Australian Breakfast Food Products

Molecules, Vol. 24, Pages 3665: Assessing the Utility of Multiplexed Liquid Chromatography-Mass Spectrometry for Gluten Detection in Australian Breakfast Food Products Molecules doi: 10.3390/molecules24203665 Authors: Li Bose Stockwell Howitt Colgrave Coeliac disease (CD) is an autoimmune disorder triggered by the ingestion of gluten that is associated with gastrointestinal issues, including diarrhea, abdominal pain, and malabsorption. Gluten is a general name for a class of cereal storage proteins of wheat, barley, and rye that are notably resistant to gastrointestinal digestion. After ingestion, immunogenic peptides are subsequently recognized by T cells in the gastrointestinal tract. The only treatment for CD is a life-long gluten-free diet. As such, it is critical to detect gluten in diverse food types, including those where one would not expect to find gluten. The utility of liquid chromatography-mass spectrometry (LC-MS) using cereal-specific peptide markers to detect gluten in heavily processed food types was assessed. A range of breakfast products, including breakfast cereals, breakfast bars, milk-based breakfast drinks, powdered drinks, and a savory spread, were tested. No gluten was detected by LC-MS in the food products labeled gluten-free, yet enzyme-linked immunosorbent assay (ELISA) measurement revealed inconsistencies in barley-containing products. In products containing wheat, rye, barley, and oats as labeled ingredients, gluten proteins w...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research

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I was looking back over the last 8 years since the first Wheat Belly book was released. It’s been 8 years of astounding, truly breathtaking stories of success over weight issues, health, and physical transformations that skeptics even today claim are impossible. It’s been a virtual avalanche of wonderful stories. Although I’ve seen all of them, often more than once, I could not help but be overcome with satisfaction and pride for the many, many spectacular photos and stories people have shared. So I thought it would be fun to re-post a small sample of some of these stories dating back from the start of th...
Source: Wheat Belly Blog - Category: Cardiology Authors: Tags: Wheat Belly Success Stories grain-free Inflammation Source Type: blogs
By just engaging in the basic strategies in the Wheat Belly Total Health, Wheat Belly 10-Day Grain Detox, or Undoctored programs, many mild cases of small intestinal bacterial overgrowth, SIBO, reverse. These efforts thereby restore your ability to ingest prebiotic fibers without diarrhea, bloating, gas, abdominal discomfort, joint pain, and dark emotional feelings. Many people thereby are relieved of irritable bowel syndrome symptoms, fibromyalgia, or restless leg syndrome, or have greater power in reversing autoimmune conditions such as rheumatoid arthritis. Unfortunately, not everybody enjoys reversal of SIBO with our b...
Source: Wheat Belly Blog - Category: Cardiology Authors: Tags: SIBO grain-free probiotic undoctored wheat belly Source Type: blogs
Several disorders related to the ingestion of gluten are well recognized despite overlapping clinical presentations: celiac disease, an autoimmune enteropathy triggered by gluten ingestions in susceptible individuals, allergy to wheat, and more recently non-celiac gluten sensitivity (NCGS). While celiac disease and wheat allergy are well-known disorders with a clear-cut diagnosis based on clinical tests and biological parameters, NCGS is a more difficult diagnosis, especially in children with functional gastrointestinal (GI) complaints. NCGS is considered a syndrome of intestinal but also extraintestinal symptoms occurring...
Source: Annals of Nutrition and Metabolism - Category: Nutrition Source Type: research
Conclusions: Our data presents an apparent trend of overlapped serological antibody biomarker positivity in RA and wheat-related disorders. PMID: 30755781 [PubMed - in process]
Source: Disease Markers - Category: Laboratory Medicine Tags: Dis Markers Source Type: research
CONCLUSIONS: This study found a high validity for MC as recorded in Swedish pathology registers. PMID: 30600733 [PubMed - as supplied by publisher]
Source: Scandinavian Journal of Gastroenterology - Category: Gastroenterology Tags: Scand J Gastroenterol Source Type: research
Abstract Celiac disease (CD) is the most common autoimmune enteropathy worldwide. In CD, dietary gluten triggers a T cell driven small intestinal inflammation in a subset of genetically predisposed subjects, expressing the HLA DQ2 and/or DQ8 genes on their antigen presenting cells. HLA DQ2/DQ8 can bind gluten peptides after their prior modification by the CD autoantigen, tissue transglutaminase (TG2). This process leads to the activation of gluten reactive T cells, small bowel villous atrophy, crypt hyperplasia and intraepithelial lymphocytosis, the histological hallmarks of CD. The clinical picture of CD is extre...
Source: European Journal of Internal Medicine - Category: Internal Medicine Authors: Tags: Eur J Intern Med Source Type: research
In the seven years since the original Wheat Belly book hit bookstores and turned the nutritional world topsy-turvy and millions of people have engaged in a grain-free lifestyle, many unique lessons have been learned. Even though I had engaged the practices of this lifestyle for a number of years and in thousands of people before I broadcast these ideas through books, expanding the audience to many more people yielded feedback on an enormous scale, new lessons that even surprised me. Among the new lessons learned along the way: Plantar fasciitis—I did not expect to have so many people report that this painful conditi...
Source: Wheat Belly Blog - Category: Cardiology Authors: Tags: News & Updates bowel flora gluten gluten-free grain-free grains Inflammation Weight Loss Source Type: blogs
CONCLUSION: We did not found celiac disease in 24 children with crFMF. Since crFMF disease is rarely seen in general population, further studies with more patients are needed to provide more precise interpretation.RESUMO CONTEXTO: A febre familiar do Mediterr âneo e a doença celíaca compartilham algumas características clínicas comuns, tais como dor abdominal, diarreia, artralgia e artrite. Além disso, ambas as doenças são associadas a muitas doenças auto-imunes e inflamatórias. Estudos anteriores mostraram associação entre febre familiar do...
Source: Arquivos de Gastroenterologia - Category: Gastroenterology Source Type: research
I don’t mean that you will be struck down by simply stepping into a Dunkin’ Donuts. I mean that donuts and others things wheat and grains will substantially abbreviate y0ur life, or at least make your time on earth a lot more miserable. I’ve been accused of exaggeration to get the no-wheat, no-grain message through. But if you see what I see every day, I think that you would agree: The consumption of wheat and grains is entirely inappropriate for humans; we exchange near-term calories for deterioration of long-term health that takes numerous forms. And when you see lives completely turned around by banish...
Source: Wheat Belly Blog - Category: Cardiology Authors: Tags: Wheat Belly Lifestyle autoimmune bowel flora diabetes gluten-free grain-free grains Inflammation joint pain undoctored Source Type: blogs
In conclusion, the novelty of this matter has generated an expansion of literature data with the unavoidable consequence that some reports are often based on low levels of evidence. Therefore, only studies performed on large samples with the inclusion of control groups will be able to clearly establish whether the large information from the literature regarding extra-intestinal NCGS manifestations could be supported by evidence-based agreements. PMID: 29662290 [PubMed - in process]
Source: World Journal of Gastroenterology : WJG - Category: Gastroenterology Authors: Tags: World J Gastroenterol Source Type: research
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