Molecular identification of the etiologic agent of human gnathostomosis in an endemic area of Mexico.

This study aims to develop a duplex-PCR method of the ITS-2 region for differentiating G. binucleatum and G. turgidum parasites that coexist in the same endemic area and to identify the Gnathostoma larvae recovered from biopsies of two gnathostomosis patients from Sinaloa, Mexico. The duplex PCR established based on the ITS-2 sequence showed that the length of the amplicons was 321 bp for G. binucleatum and 226 bp for G. turgidum. The amplicons from the AL3A of both patients were 321 bp. Furthermore, the length and composition of these amplicons were identical to that deposited in GenBank as G. binucleatum (accession No. JF919679), corroborating our previous morphological finding that G. binucleatum is the etiological agent for human gnathostomosis in an endemic area of Sinaloa, Mexico. PMID: 31564694 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research