Molecular identification of the etiologic agent of human gnathostomosis in an endemic area of Mexico.

This study aims to develop a duplex-PCR method of the ITS-2 region for differentiating G. binucleatum and G. turgidum parasites that coexist in the same endemic area and to identify the Gnathostoma larvae recovered from biopsies of two gnathostomosis patients from Sinaloa, Mexico. The duplex PCR established based on the ITS-2 sequence showed that the length of the amplicons was 321 bp for G. binucleatum and 226 bp for G. turgidum. The amplicons from the AL3A of both patients were 321 bp. Furthermore, the length and composition of these amplicons were identical to that deposited in GenBank as G. binucleatum (accession No. JF919679), corroborating our previous morphological finding that G. binucleatum is the etiological agent for human gnathostomosis in an endemic area of Sinaloa, Mexico. PMID: 31564694 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research

Related Links:

AbstractWith sand flies as the main vectors,Leishmania species cause ancient zoonotic diseases called leishmaniasis. Iran is an endemic country regarding cutaneous leishmaniasis. A number of 100 smear slides were collected from cutaneous lesions referred to Ahvaz health centers. The DNA was extracted and ITS1 –PCR using LITSR and L5.8S primer pair was performed to detect the genusLeishmania. Then, enzymatic digestion of PCR products was done byHaeIII (species detection),TaqI (strain detection),DpnI andHpaII (mutation assessment). Furthermore, 50 samples were sent for sequencing. Microscopic examination showed amastig...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research
Publication date: Available online 22 January 2020Source: International Journal for Parasitology: Parasites and WildlifeAuthor(s): Ronel Pienaar, P. Christo Troskie, Antoinette I. Josemans, Fred T. Potgieter, Boitumelo B. Maboko, Abdalla A. Latif, Ben J. MansAbstractThe Theileria are apicomplexan parasites transmitted by ticks to vertebrate hosts. Most Theileria species exhibit some form of host or vector specificity, since under endemic conditions only a limited number of tick species act as vectors and not all vertebrate hosts are able to maintain a persistent carrier state. Data for Theileria sp. (buffalo) suggest host ...
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
Conclusions/SignificanceLoratadine, cyproheptadine and clofazimine inhibit TcAAAP069 proline transporter and also present trypanocidal effect against allT.cruzi life stages in strains from three different DTUs. These CV structural analogues could be a starting point to design therapeutic alternatives to treat Chagas disease by finding new indications for old drugs. This approach, called drug repurposing is a recommended strategy by the World Health Organization to treat neglected diseases, like Chagas disease, and combination therapy may improve the possibility of success of repositioned drugs.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Authors: Lizardo K, Ayyappan JP, Ganapathi U, Dutra WO, Qiu Y, Weiss LM, Nagajyothi JF Abstract Chagas disease is caused by Trypanosoma cruzi which is endemic in Latin America. T. cruzi infection results in a latent infection with approximately a third of latently infected patients developing chronic Chagas cardiomyopathy (CCM). CCM is a common cause of cardiomyopathy in endemic regions and has a poor prognosis compared to other cardiomyopathies. The factors responsible for the transition from the asymptomatic indeterminate latent stage of infection to CCM are poorly understood. Our previous studies demonstrated th...
Source: Disease Markers - Category: Laboratory Medicine Tags: Dis Markers Source Type: research
Publication date: Available online 17 January 2020Source: Parasitology InternationalAuthor(s): Toshihiro Tokiwa, Hisashi Yoshimura, Keiko Ito, Shyun Chou, Masami YamamotoAbstractThere is limited knowledge about parasites of the endangered Ryukyu long-furred rat, Diplothrix legata (Murinae, Rodentia) endemic to Okinawa, Tokunoshima, and Amami-Oshima Islands in Japan. In the present study, postmortem histopathological examination of an individual found on Amami-Oshima Island revealed a mixed helminth infection of Calodium hepaticum, Hydatigera taeniaeformis, and Angiostrongylus cantonensis. These helminths are considered non...
Source: Parasitology International - Category: Parasitology Source Type: research
In this study, we aimed to investigate phlebovirus and Leishmania parasites circulating in a focus of zoonotic visceral leishmaniasis (ZVL) located in a highly irrigated area within the arid Central Tunisia, known mainly to be endemic for zoonotic cutaenous leishmaniasis (ZCL) caused Leishmania major and transmitted by Phlebotomus papatasi. Sand flies were collected using CDC light traps in the village of Saddaguia, an emergent focus of ZVL located in Central Tunisia during September-October 2014, 2015, and 2016. Pools of live female sand flies were screened for phleboviruses and Leishmania by nested PCR in the polymerase ...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research
We conducted a matched-pairs trial of three methods for detecting house infestation with triatominae bugs in a well-defined endemic rural area in the Argentine Chaco.
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
Leishmaniasis, considered by the World Health Organization as one of the most important tropical diseases, is endemic in the Mediterranean Basin. The aim of this study was to evaluate epidemiological and clini...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
Conclusions/SignificanceThis study established satisfactory results inLeishmania parasite clearing bothin-vivo andin-vitro. Therefore,U.dioica extract can be considered as an effective and harmless herbal compound for killing the parasite without toxicity to the host macrophages. Furthermore, it also can treat the CL by switching the mouse immune response towards a cell-mediated response (Th1); hence, it may be identified as a perfect therapeutic herbal drug for CL treatment.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
We present a rare case of paragonimiasis masquerading as hypereosinophilia, asthma, and intracerebral hemorrhage for the last three years. Paragonimiasis is a rare cause of hemoptysis outside endemic regions. A strong index of suspicion is required to diagnose paragonimiasis outside the endemic areas. Diagnosis is usually delayed because of similarity of presentation to TB. Our patient was symptomatic with hemoptysis for three years. He gave history of eating raw crayfish and crabs while on leave to his native village in Nagaland. Diagnosis was confirmed on microscopy by detection of Paragonimus ova in sputum and bronchoal...
Source: Medical Journal Armed Forces India - Category: General Medicine Source Type: research
More News: Allergy & Immunology | Endemics | Epidemiology | Fish | Infectious Diseases | Japan Health | Meat | Mexico Health | Parasitic Diseases | Parasitology | Science | Study