Prevalence of antibiotic resistant mastitis pathogens in dairy cows in Egypt and potential biological control agents produced from plant endophytic actinobacteria

Publication date: Available online 14 September 2019Source: Saudi Journal of Biological SciencesAuthor(s): Fuad Ameen, Shorouk A. Reda, Sahar A. El-Shatoury, Emad M. Riad, Mohamed E. Enany, Abdullah A. AlarfajAbstractDairy production is threatened by antibiotic resistant pathogens worldwide, and alternative solutions to treat mastitis are not available. The prevalence of antibiotic resistant strains is not well known in less developed countries. The prevalence of pathogenic bacteria and their resistance to 21 commercial antibiotics were studied in milk samples taken from 122 dairy cows suffering from the symptoms of mastitis in Egypt. The bacterial species were identified with molecular methods, and antibiotic resistance was studied with disc diffusion method. The prevalence of Streptococcus aureus, Escherichia coli and Pseudomonas aeruginosa were 30%, 17% and 3.5%, respectively. Most (90%) of the S. aureus strains showed resistance to penicillin whereas only 10% of the strains were resistant to oxacillin. Nearly half (40%) of E. coli strains showed resistance to streptomycin. Six P. aeruginosa strains showed resistance to several antibiotics, including ceftriaxone, enrofloxacin and levofloxacin. This points out that despite P. aeruginosa was not common, it should be followed up carefully. Potential biocontrol agents against antibiotic resistant mastitis bacteria were searched among 30 endophytic actinobacterial strains derived from wild medicinal plants. Three plants, namely...
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research

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ConclusionThis review shows that CAM may be useful for ALS treatment, but more evidence regarding the efficacy and molecular mechanisms is required to establish CAM as a good therapy for the treatment of ALS patients.
Source: Integrative Medicine Research - Category: Complementary Medicine Source Type: research
Publication date: Available online 18 October 2019Source: Acta Pharmaceutica Sinica BAuthor(s): Yuhua Li, Qiang Meng, Meng biYang, Dongyang Liu, Xiangyu Hou, Lan Tang, Xin Wang, Yuanfeng Lyu, Xiaoyan Chen, Kexin Liu, Ai-Ming Yu, Zhong Zuo, Huichang BiAbstractPharmacokinetics (PK) is the study of the absorption, distribution, metabolism, and excretion (ADME) processes of a drug. Understanding PK properties is essential for drug development and precision medication. In this review we provided an overview of recent research on PK with focus on the following aspects: (1) an update on drug-metabolizing enzymes and transporters ...
Source: Acta Pharmaceutica Sinica B - Category: Cancer & Oncology Source Type: research
Publication date: December 2019Source: Molecular Immunology, Volume 116Author(s): Mahya Ranjbar, Bahador Behrouz, Fatemeh Norouzi, Seyed Latif Mousavi GargariAbstractPseudomonas aeruginosa is a common nosocomial pathogen in burn patients, and rapidly acquires antibiotic resistance; thus, developing an effective therapeutic approach is the most promising strategy for combating infection. Type III secretion system (T3SS) translocates bacterial toxins into the cytosol of the targeted eukaryotic cells, which plays important roles in the virulence of P. aeruginosa infections in both acute pneumonia and burn wound models. The Pc...
Source: Molecular Immunology - Category: Allergy & Immunology Source Type: research
Publication date: December 2019Source: Molecular Immunology, Volume 116Author(s): Mokhtar Nosrati, Abbas Hajizade, Shahram Nazarian, Jafar Amani, Amir Namvar Vansofla, Yousof TarverdizadehAbstractShigellosis is a severe diarrheal disease with high mortality and morbidity rate. Until now, there is no approved vaccine against the disease. Therefore, the present study was planned to design a novel multi-epitope vaccine against Shigella spp., the causative agents of the disease based on the immunoinformatic tools. For this end, firstly seven conserved antigens of the bacteria, including IpaA, IpaB, IpaC, IpaD, OmpC, OmpF and V...
Source: Molecular Immunology - Category: Allergy & Immunology Source Type: research
This study shows that dual species biofilms with foodborne pathogens are more resistant to disinfectant treatment than their single species counterparts and that biofilm maturity as well as environmental conditions, i.e. access to nutrients, play an important role in their response to the treatments tested.
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
In this study, five fractions (F–I to F–V) were successfully isolated from cottonseed protein hydrolysates (CPH) by using ion exchange resin system, and proved to be mainly composed of peptides with molecular weights less than 1 kDa by size exclusion high performance liquid chromatography (SEC-HPLC). All fractions possessed the DPPH radical and ABTS radical cation scavenging abilities. However, the antibacterial experiment revealed F–I and CPH had no inhibition against E. coli. By analyzing the composition of amino acids, the antioxidant and antibacterial activities were found to be positively correlate...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Publication date: January 2020Source: LWT, Volume 118Author(s): Salma Ben-Harb, Françoise Irlinger, Anne Saint-Eve, Maud Panouillé, Isabelle Souchon, Pascal BonnarmeAbstractThe use of pea protein is limited in food by the persistence of off-flavours. Fermentation by microbial consortia could be a way to circumvent this problem. The aim of our study was to investigate the adaptation potential and metabolic features of consortia as a function of the matrix composition (pea and/or milk proteins). Three designed consortia were investigated for their ability (i) to grow in gels containing 100% pea proteins, 50% pe...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Publication date: January 2020Source: Food Research International, Volume 127Author(s): Zuhaib F. Bhat, James D. Morton, Xin Zhang, Susan L. Mason, Alaa El-Din A. BekhitAbstractEffect of sous-vide processing was evaluated on in-vitro simulated protein digestion, myofibrillar protein profile, lipid oxidation and physicochemical properties of cold-boned beef Semitendinosus. Sous-vide processed [T1, 60 °C (4.5 h); T2, 60 °C (10 h)] samples along with control beef (cooked at 80 °C until a core temperature of 75 °C was attained) were subjected to in-vitro simulated gastrointestinal protein digestion....
Source: Food Research International - Category: Food Science Source Type: research
Abstract Mastitis caused by multi- or pan-drug resistant bacteria is a growing health concern. A total of 110 milk samples were collected: Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Enterococcus faecalis, and Escherichia coli were present in 54/110 (49.09%), 37/110 (33.63%), 25/110 (22.72%), 7/110 (6.36%), and 50/110 (45.45%) samples, respectively. A total of 20 methicillin-resistant S. aureus (MRSA) isolates, 19 Streptococcus sp. isolates, and 15 E. coli isolates were selected, and 100% were positive for (coagulase and hemolysins), streptokinase, and hemolytic activity, respectiv...
Source: Molecular Biology Reports - Category: Molecular Biology Authors: Tags: Mol Biol Rep Source Type: research
Conclusion The results of our study demonstrate that robenidine is not suitable as a sole antimicrobial agent for the treatment of Gram-negative pathogen infections due to the lack of activity against the majority of Gram-negative isolates except for A. baumannii and A. calcoaceticus. However, we demonstrated in vitro efficacy against all selected Gram-negative organisms when robenidine was tested in combination with EDTA or PMBN, including against multidrug-resistant strains. Therefore, robenidine may be an appropriate candidate as a component of a combination preparation for the treatment of otitis externa in dogs. This...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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