Dual drug loaded PLGA nanospheres for synergistic efficacy in breast cancer therapy

Publication date: October 2019Source: Materials Science and Engineering: C, Volume 103Author(s): Murugesan Sathiya Deepika, Ramar Thangam, Thankaraj Salammal Sheena, R.T.V. Vimala, Srinivasan Sivasubramanian, Kulandaivel Jeganathan, Ramasamy ThirumuruganAbstractImproved therapeutic effects can be achieved by the delivery of combination of drugs through multifunctional cell targeted nanocarrier systems. The present investigation reports the preparation of Poly (D,L-lactic-co-glycolic acid) (PLGA) nanospheres loaded with the novel combination such as Rutin (R) and Benzamide (B) as drugs using water-oil-water (w/o/w) emulsion method. Dual drug loaded PLGA nanospheres (R/B@PLGA) were stabilized by poly (vinyl alcohol) (PVA) coating and characterized in terms of morphology, size, surface charge, and structural chemistry by Scanning electron microscopy (SEM), Dynamic light scattering (DLS), Zeta potential analysis, UV–vis and Fourier transform infrared (FT-IR) spectroscopy. The inhibitory effects of rutin and benzamide on MDA-MB-231 (triple negative breast cancer-TNBC) cells using the drug loaded PLGA nanospheres as well as their non-toxic features were evaluated in vivo. The anticancer activity of the R/B@PLGA nanospheres through cell cycle disruption and apoptotic induction was assessed in vitro by flow cytometry analysis. Further, the in vitro antioxidant capacity, pH-based drug release and hemocompatible property were also investigated. It was shown that the R/B@PLGA nanosphe...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research