Green silver nanoparticles from novel Brassicaceae cultivars with enhanced antimicrobial potential than earlier reported Brassicaceae members

Publication date: Available online 12 January 2018 Source:Journal of Trace Elements in Medicine and Biology Author(s): Amarjeet Singh, Bhavana Sharma, Renu Deswal In the present study, we report perhaps for the first time the use of novel varieties of Brassica oleracea var. botrytis and Raphanus sativus as potential bioreductant to synthesize highly stable silver nanoparticles (AgNPs, no aggregation observed for six months), which is a significant finding as plant extract-directed Ag NPs are intrinsically unstable and tend to aggregate. The reduction of Ag+ to Ag0 nanostructures was confirmed using UV–Visible spectroscopy showing SPR spectra at 400–435 nm. Nanosight and transmission electron microscope (TEM) analysis showed monodisperse spherical AgNPs (4–18 nm). Fourier transform infrared spectroscopy (FTIR) analysis revealed that the polyphenolics and other secondary metabolites including glucosinolates in the aqueous extracts may act as reducing/capping agent for the nanoparticle synthesis. X-ray diffraction (XRD) confirmed the face centered cubic crystalline (fcc) structure of AgNPs. Controlled synthesis of AgNPs was achieved by varying experimental parameters (AgNO3 concentration, extract volume, pH and temperature). AgNPs exhibited strong antibacterial activity at significantly lower concentration (5 ppm) against both Gram negative (Escherichia coli, Myroides, Psuedomonas aeruginosa) and Gram positive (Kocuria, and Promicromonospora) bacteria. In the pr...
Source: Journal of Trace Elements in Medicine and Biology - Category: Biochemistry Source Type: research