Neuronal cell sheet of cortical motor neuron phenotype derived from human iPS cells.

Neuronal cell sheet of cortical motor neuron phenotype derived from human iPS cells. Cell Transplant. 2017 Mar 17;: Authors: Suzuki N, Arimitsu N, Shimizu J, Takai K, Hirotsu C, Takada E, Ueda Y, Wakisaka S, Fujiwara N, Suzuki T Abstract <p>Transplantation of stem cells which differentiate into more mature neural cells brings about functional improvement in pre-clinical studies of stroke. Previous transplant approaches in diseased brain have utilized injection of the cells in a cell suspension. In addition, neural stem cells were preferentially used as graft. However, these cells had no specific relationship to the damaged tissue of stroke patients and brain injury. The injection of cells in a suspension destroyed the cell-cell interactions that are suggested to be important for promoting functional integrity as cortical motor neurons.</p><p>In order to obtain suitable cell types for grafting patients with stroke and brain damage, we have modified a protocol for differentiating human iPS cells to cells phenotypically related to cortical motor neurons. Moreover, we applied cell sheet technology to neural cell transplantation due to the idea in which keeping cell-cell communications was regarded as important for the repair of host brain architecture.</p><p>Accordingly, we developed neuronal cell sheets being positive for FEZ family zinc finger 2 (Fezf2), COUP-TF-interacting protein 2 (CTIP2), insulin-like ...
Source: Cell Transplantation - Category: Cytology Authors: Tags: Cell Transplant Source Type: research