Abstract 1980: Identification of the transcription factor ZNF217 in ER+ subset of BC: A functional relationship with the PI3K-AKT-mTOR pathway

ZNF217 gene encodes for a Krüppel-like finger protein transcription factor. Here we report an amplification of ZNF217 gene in breast cancer (BC) patients. Retrospective study of 72 BC patients showed that ZNF217 gene was amplified in 12.5% of patients enrolled in our center over last ten months from February 2014 through November 2014. We found a similar level of amplification of ZNF217 gene at 20q13 in different epithelial cancers including lung, uterus, ovary, stomach, bladder and breast using data from c-Bioportal. The observed percentage of the amplification of ZNF217 gene in our patients (12.5%) was comparable to the c-BioPortal data which showed that this gene is altered (amplification, mutation, heterozygous deletion) in 12.1% (Breast Invasive Carcinoma; TCGA, Provisional; tumors with sequencing and CNA data; 962 samples) and in 13.3% (Breast Invasive Carcinoma; TCGA, Nature 2012; 825 samples) of all cases. In contrast to the cBioPortal data, we have observed only an amplification of ZNF217 gene in our patient samples. Amplification of ZNF217 gene was predominantly encountered in our ER+ patients (16.6%). Similarly 11% of the luminal A patients showed alterations in the ZNF217 gene while 24% of the luminal B showed alterations in the ZNF217 (Breast Invasive Carcinoma; TCGA, Nature 2012; 235 luminal A samples cBioPortal). A comparable trend of higher mRNA expression (DASL analyses from FFPE; Dey et al. BMC Cancer 2013) for ZNF217 was also observed in the ER+/PR+/HER2+...

Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Dey, N., Williams, C., Krie, A., Solomon, B., Starks, D., Rojas, L., Carlson, J. H., Sun, Y., Lin, X., Abramovitz, M., Metzger-Nelson, T., Williams, K., Klein, J., De, P., Leyland-Jones, B. Tags: Molecular and Cellular Biology Source Type: research