A lupin protein hydrolysate protects the central nervous system from oxidative stress in WD ‐fed ApoE−/− mice

A Lupinus angustifolius protein hydrolysate (LPH), containing 101 sequences with antioxidant activity, improves the antioxidant status in vitro and in the brain of mice after 12 weeks of treatment. Specifically, LPH decreases the inducible nitric oxide synthase (iNOS) gene expression, nitric oxide (NO) and 8-hydroxy-2 ’-deoxyguanosine (8-OHdG) production, and increases catalase (CAT) and glutathione reductase (GR) activities and glutathione (GSH) levels. AbstractOxidative stress plays a crucial role in neurodegenerative diseases like Parkinson's and Alzheimer's. Studies indicate the relationship between oxidative stress and the brain damage caused by a high-fat diet. It is previously found that a lupin protein hydrolysate (LPH) has antioxidant effects on human leukocytes, as well as on the plasma and liver of Western diet (WD)-fed ApoE−/− mice. Additionally, LPH shows anxiolytic effects in these mice. Given the connection between oxidative stress and anxiety, this study aimed to investigate the antioxidant effects of LPH on the brain of WD-fed ApoE−/− mice. LPH (100 mg kg−1) or a vehicle is administered daily for 12 weeks. Peptide analysis of LPH identified 101 amino acid sequences (36.33%) with antioxidant motifs. Treatment with LPH palliated the decrease in total antioxidant activity caused by WD ingestion and regulated the nitric oxide synthesis pathway in the brain of the animals. Furthermore, LPH increased cerebral glutathione levels and the activity of catal...
Source: Molecular Nutrition and Food Research - Category: Food Science Authors: Tags: Research Article Source Type: research