Single and mixture toxicity evaluation of avobenzone and homosalate to male zebrafish and H295R cells

In this study, the effect of the toxicity of a binary mixture comprising avobenzone (0.72 μg L-1) and homosalate (1.02 and 103 μg L-1) on steroid hormone biosynthesis were investigated using male zebrafish and human adrenocortical carcinoma (H295R) cells. In fish exposed to homosalate, a significant decrease in the gonadosomatic index, testosterone level, and transcription of several genes (e.g, hsd3b2, cyp17a1, and hsd17b1) and a significant increase in the hepatosomatic index, liver steatosis, 17β-estradiol level, and transcription of vtg gene were observed. These results suggest that estrogenic and anti-androgenic effects of homosalate were mediated by the steroidogenic pathway. The presence of 0.72 μg L-1 of avobenzone augmented the anti-androgenic responses in male fish. The testosterone level in the H295R cells were significantly decreased after they were exposed to homosalate alone or in combination with avobenzone, which is consistent with observations in male zebrafish. Further studies need to be conducted to understand the endocrine disrupting properties of long-term exposure to substances typically used in sunscreens.PMID:37758070 | DOI:10.1016/j.chemosphere.2023.140271
Source: Chemosphere - Category: Chemistry Authors: Source Type: research