Protein engineering for the synthetic cell factory: Recent advance and perspective
Biotechnol Adv. 2024 Apr 23:108366. doi: 10.1016/j.biotechadv.2024.108366. Online ahead of print.ABSTRACTSynthetic cell factory offers substantial advantages in economically efficient production of biofuels, chemicals, and pharmaceutical compounds. However, to create a high-performance synthetic cell factory, precise regulation of cellular material and energy flux is essential. In this context, protein components including enzymes, transcription factor-based biosensors and transporters play pivotal roles. Protein engineering is a technology for creating novel protein variants with desired properties and functions by modify...
Source: Biotechnology Advances - April 25, 2024 Category: Biotechnology Authors: Ailin Guan Zixi He Xin Wang Zhi-Jun Jia Jiufu Qin Source Type: research
Biobased short chain fatty acid production - Exploring microbial community dynamics and metabolic networks through kinetic and microbial modeling approaches
Biotechnol Adv. 2024 Apr 22;73:108363. doi: 10.1016/j.biotechadv.2024.108363. Online ahead of print.ABSTRACTIn recent years, there has been growing interest in harnessing anaerobic digestion technology for resource recovery from waste streams. This approach has evolved beyond its traditional role in energy generation to encompass the production of valuable carboxylic acids, especially volatile fatty acids (VFAs) like acetic acid, propionic acid, and butyric acid. VFAs hold great potential for various industries and biobased applications due to their versatile properties. Despite increasing global demand, over 90% of VFAs a...
Source: Biotechnology Advances - April 24, 2024 Category: Biotechnology Authors: Merve Atasoy William T Scott Alberte Regueira Miguel Mauricio-Iglesias Peter J Schaap Hauke Smidt Source Type: research
Optimization strategies for CO < sub > 2 < /sub > biological fixation
This article reviews recent advances in optimizing CO2 biological fixation and intends to provide new insights into achieving efficient biological utilization of CO2. It first outlines the thermodynamic characteristics of diverse carbon fixation reactions and proposes optimization directions for CO2 biological fixation. A comprehensive overview of the catalytic mechanisms, optimization strategies, and challenges encountered by common carbon-fixing enzymes is then provided. Subsequently, potential routes for improving the efficiency of biological carbon fixation are discussed, including the ATP supply, reducing power supply...
Source: Biotechnology Advances - April 20, 2024 Category: Biotechnology Authors: Xiutao Liu Linqing Li Guang Zhao Peng Xiong Source Type: research
Optimization strategies for CO < sub > 2 < /sub > biological fixation
This article reviews recent advances in optimizing CO2 biological fixation and intends to provide new insights into achieving efficient biological utilization of CO2. It first outlines the thermodynamic characteristics of diverse carbon fixation reactions and proposes optimization directions for CO2 biological fixation. A comprehensive overview of the catalytic mechanisms, optimization strategies, and challenges encountered by common carbon-fixing enzymes is then provided. Subsequently, potential routes for improving the efficiency of biological carbon fixation are discussed, including the ATP supply, reducing power supply...
Source: Biotechnology Advances - April 20, 2024 Category: Biotechnology Authors: Xiutao Liu Linqing Li Guang Zhao Peng Xiong Source Type: research
The dawning era of oral thin films for nutraceutical delivery: From laboratory to clinic
Biotechnol Adv. 2024 Apr 12;73:108362. doi: 10.1016/j.biotechadv.2024.108362. Online ahead of print.ABSTRACTOral thin films (OTFs) are innovative dosage forms that have gained tremendous attention for the delivery of nutraceuticals. They are ultra-thin, flexible sheets that can be easily placed on the tongue, sublingual or buccal mucosa (inner lining of the cheek). These thin films possess several advantages for nutraceutical delivery including ease of administration, rapid disintegration, fast absorption, rapid onset of action, bypass first-pass hepatic metabolism, accurate dosing, enhanced stability, portability, discree...
Source: Biotechnology Advances - April 14, 2024 Category: Biotechnology Authors: None Ruchika Nabab Khan Shagun Sanjivv Dogra Ankit Saneja Source Type: research
The dawning era of oral thin films for nutraceutical delivery: From laboratory to clinic
Biotechnol Adv. 2024 Apr 12:108362. doi: 10.1016/j.biotechadv.2024.108362. Online ahead of print.ABSTRACTOral thin films (OTFs) are innovative dosage forms that have gained tremendous attention for the delivery of nutraceuticals. They are ultra-thin, flexible sheets that can be easily placed on the tongue, sublingual or buccal mucosa (inner lining of the cheek). These thin films possess several advantages for nutraceutical delivery including ease of administration, rapid disintegration, fast absorption, rapid onset of action, bypass first-pass hepatic metabolism, accurate dosing, enhanced stability, portability, discreetne...
Source: Biotechnology Advances - April 14, 2024 Category: Biotechnology Authors: None Ruchika Nabab Khan Shagun Sanjivv Dogra Ankit Saneja Source Type: research
The dawning era of oral thin films for nutraceutical delivery: From laboratory to clinic
Biotechnol Adv. 2024 Apr 12:108362. doi: 10.1016/j.biotechadv.2024.108362. Online ahead of print.ABSTRACTOral thin films (OTFs) are innovative dosage forms that have gained tremendous attention for the delivery of nutraceuticals. They are ultra-thin, flexible sheets that can be easily placed on the tongue, sublingual or buccal mucosa (inner lining of the cheek). These thin films possess several advantages for nutraceutical delivery including ease of administration, rapid disintegration, fast absorption, rapid onset of action, bypass first-pass hepatic metabolism, accurate dosing, enhanced stability, portability, discreetne...
Source: Biotechnology Advances - April 14, 2024 Category: Biotechnology Authors: None Ruchika Nabab Khan Shagun Sanjivv Dogra Ankit Saneja Source Type: research
The dawning era of oral thin films for nutraceutical delivery: From laboratory to clinic
Biotechnol Adv. 2024 Apr 12:108362. doi: 10.1016/j.biotechadv.2024.108362. Online ahead of print.ABSTRACTOral thin films (OTFs) are innovative dosage forms that have gained tremendous attention for the delivery of nutraceuticals. They are ultra-thin, flexible sheets that can be easily placed on the tongue, sublingual or buccal mucosa (inner lining of the cheek). These thin films possess several advantages for nutraceutical delivery including ease of administration, rapid disintegration, fast absorption, rapid onset of action, bypass first-pass hepatic metabolism, accurate dosing, enhanced stability, portability, discreetne...
Source: Biotechnology Advances - April 14, 2024 Category: Biotechnology Authors: None Ruchika Nabab Khan Shagun Sanjivv Dogra Ankit Saneja Source Type: research
Microbial production of sulfur-containing amino acids using metabolically engineered Escherichia coli
Biotechnol Adv. 2024 Apr 7;73:108353. doi: 10.1016/j.biotechadv.2024.108353. Online ahead of print.ABSTRACTL-Cysteine and L-methionine, as the only two sulfur-containing amino acids among the canonical 20 amino acids, possess distinct characteristics and find wide-ranging industrial applications. The use of different organisms for fermentative production of L-cysteine and L-methionine is gaining increasing attention, with Escherichia coli being extensively studied as the preferred strain. This preference is due to its ability to grow rapidly in cost-effective media, its robustness for industrial processes, the well-charact...
Source: Biotechnology Advances - April 9, 2024 Category: Biotechnology Authors: Lijuan Wang Yingying Guo Yizhou Shen Kun Yang Xue Cai Bo Zhang Zhiqiang Liu Yuguo Zheng Source Type: research
Microbial production of sulfur-containing amino acids using metabolically engineered Escherichia coli
Biotechnol Adv. 2024 Apr 7;73:108353. doi: 10.1016/j.biotechadv.2024.108353. Online ahead of print.ABSTRACTL-Cysteine and L-methionine, as the only two sulfur-containing amino acids among the canonical 20 amino acids, possess distinct characteristics and find wide-ranging industrial applications. The use of different organisms for fermentative production of L-cysteine and L-methionine is gaining increasing attention, with Escherichia coli being extensively studied as the preferred strain. This preference is due to its ability to grow rapidly in cost-effective media, its robustness for industrial processes, the well-charact...
Source: Biotechnology Advances - April 9, 2024 Category: Biotechnology Authors: Lijuan Wang Yingying Guo Yizhou Shen Kun Yang Xue Cai Bo Zhang Zhiqiang Liu Yuguo Zheng Source Type: research
Microbial production of sulfur-containing amino acids using metabolically engineered Escherichia coli
Biotechnol Adv. 2024 Apr 7:108353. doi: 10.1016/j.biotechadv.2024.108353. Online ahead of print.ABSTRACTL-Cysteine and L-methionine, as the only two sulfur-containing amino acids among the canonical 20 amino acids, possess distinct characteristics and find wide-ranging industrial applications. The use of different organisms for fermentative production of L-cysteine and L-methionine is gaining increasing attention, with Escherichia coli being extensively studied as the preferred strain. This preference is due to its ability to grow rapidly in cost-effective media, its robustness for industrial processes, the well-characteri...
Source: Biotechnology Advances - April 9, 2024 Category: Biotechnology Authors: Lijuan Wang Yingying Guo Yizhou Shen Kun Yang Xue Cai Bo Zhang Zhiqiang Liu Yuguo Zheng Source Type: research
Microbial production of sulfur-containing amino acids using metabolically engineered Escherichia coli
Biotechnol Adv. 2024 Apr 7:108353. doi: 10.1016/j.biotechadv.2024.108353. Online ahead of print.ABSTRACTL-Cysteine and L-methionine, as the only two sulfur-containing amino acids among the canonical 20 amino acids, possess distinct characteristics and find wide-ranging industrial applications. The use of different organisms for fermentative production of L-cysteine and L-methionine is gaining increasing attention, with Escherichia coli being extensively studied as the preferred strain. This preference is due to its ability to grow rapidly in cost-effective media, its robustness for industrial processes, the well-characteri...
Source: Biotechnology Advances - April 9, 2024 Category: Biotechnology Authors: Lijuan Wang Yingying Guo Yizhou Shen Kun Yang Xue Cai Bo Zhang Zhiqiang Liu Yuguo Zheng Source Type: research
Microbial production of sulfur-containing amino acids using metabolically engineered Escherichia coli
Biotechnol Adv. 2024 Apr 7:108353. doi: 10.1016/j.biotechadv.2024.108353. Online ahead of print.ABSTRACTL-Cysteine and L-methionine, as the only two sulfur-containing amino acids among the canonical 20 amino acids, possess distinct characteristics and find wide-ranging industrial applications. The use of different organisms for fermentative production of L-cysteine and L-methionine is gaining increasing attention, with Escherichia coli being extensively studied as the preferred strain. This preference is due to its ability to grow rapidly in cost-effective media, its robustness for industrial processes, the well-characteri...
Source: Biotechnology Advances - April 9, 2024 Category: Biotechnology Authors: Lijuan Wang Yingying Guo Yizhou Shen Kun Yang Xue Cai Bo Zhang Zhiqiang Liu Yuguo Zheng Source Type: research
Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids
Biotechnol Adv. 2024 Apr 6:108354. doi: 10.1016/j.biotechadv.2024.108354. Online ahead of print.ABSTRACTThraustochytrids are marine microorganisms known for their fast growth and ability to store lipids, making them useful for producing polyunsaturated fatty acids (PUFAs), biodiesel, squalene, and carotenoids. However, the high cost of production, mainly due to expensive fermentation components, limits their wider use. A significant challenge in this context is the need to balance production costs with the value of the end products. This review focuses on integrating the efficient utilization of waste with Thraustochytrids...
Source: Biotechnology Advances - April 8, 2024 Category: Biotechnology Authors: Zi-Xu Zhang Ying-Shuang Xu Zi-Jia Li Lu-Wei Xu Wang Ma Ying-Feng Li Dong-Sheng Guo Xiao-Man Sun He Huang Source Type: research
Recent advances in design and application of synthetic membraneless organelles
Biotechnol Adv. 2024 Apr 6:108355. doi: 10.1016/j.biotechadv.2024.108355. Online ahead of print.ABSTRACTMembraneless organelles (MLOs) formed by liquid-liquid phase separation (LLPS) have been extensively studied due to their spatiotemporal control of biochemical and cellular processes in living cells. These findings have provided valuable insights into the physicochemical principles underlying the formation and functionalization of biomolecular condensates, which paves the way for the development of versatile phase-separating systems capable of addressing a variety of application scenarios. Here, we highlight the potentia...
Source: Biotechnology Advances - April 8, 2024 Category: Biotechnology Authors: Li Wan Yingying Zhu Wenli Zhang Wanmeng Mu Source Type: research
