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Specialty: Biochemistry
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Total 111164 results found since Jan 2013.
Development of Thiol-Ene Reaction-Based HA Hydrogel with Sustained Release of EGF for Enhanced Skin Wound Healing
This study develops a novel drug delivery system using a hyaluronic acid (HA) hydrogel for controlled release of epidermal growth factor (EGF) to enhance skin wound healing. Conventional hydrogel-based methods suffer from a burst release and limited drug delivery times. To address this, we employ bioconjugation to introduce an acrylate group to EGF, enabling chemical bonding to the HA hydrogel matrix through thiol-ene cross-linking. This approach results in sustained-release delivery of EGF based on the degradation rate of the HA matrix, overcoming diffusion-based limitations. We confirm the introduction of the acrylate gr...
Source: Biomacromolecules - September 21, 2023 Category: Biochemistry Authors: Yerin Lee Saebin Lim Ji An Kim Yoon Hong Chun Hyun Jong Lee Source Type: research
Multifunctional Polymer Vesicles for Synergistic Antibiotic-Antioxidant Treatment of Bacterial Keratitis
Biomacromolecules. 2023 Sep 21. doi: 10.1021/acs.biomac.3c00754. Online ahead of print.ABSTRACTAs an acute ophthalmic infection, bacterial keratitis (BK) can lead to severe visual morbidity, such as corneal perforation, intraocular infection, and permanent corneal opacity, if rapid and effective treatments are not available. In addition to eradicating pathogenic bacteria, protecting corneal tissue from oxidative damage and promoting wound healing by relieving inflammation are equally critical for the efficient treatment of BK. Besides, it is very necessary to improve the bioavailability of drugs by enhancing the ocular sur...
Source: Biomacromolecules - September 21, 2023 Category: Biochemistry Authors: Qiumeng Chen Xiaopeng Han Lu Liu Yong Duan Yifei Chen Linqi Shi Quankui Lin Liangliang Shen Source Type: research
Tannin-Assisted Synthesis of Nanocomposites Loaded with Silver Nanoparticles and Their Multifunctional Applications
Biomacromolecules. 2023 Sep 21. doi: 10.1021/acs.biomac.3c00737. Online ahead of print.ABSTRACTNanocomposites have been widely used in many important areas due to their particular physical/chemical properties; however, just though a simple technology, endowing multiple functions into a single nanomaterial for realizing their multifunctional applications is still a challenge. Here, we report a robust method for the facile synthesis of Ag-based multifunctional nanocomposites via using tannin-coated phenol-formaldehyde resin nanospheres (TA-PFRN) as silver nanoparticle (Ag NP) carriers. The thickness of the tannin coating is ...
Source: Biomacromolecules - September 21, 2023 Category: Biochemistry Authors: Mengyao Xia Weikun Jiang Chen Wu Chao Wang Chang Geun Yoo Yu Liu Gaojin Lyu Source Type: research
D-pinitol ameliorated H < sub > 2 < /sub > O < sub > 2 < /sub > -induced oxidative damage in PC12 cells and prolonged the lifespan by IIS pathway in Caenorhabditis elegans
In this study, we intended to explore the anti-aging mechanism of DP, utilizing computer modeling techniques. The results demonstrated that DP significantly delayed H2O2-induced cellular senescence. Model PC12 cells treated with DP exhibited increased cell viability, increased antioxidant enzyme activity (SOD, CAT), and reduced ROS and MDA levels. Furthermore, DP was discovered to have a positive effect on healthy longevity. In C. elegans, DP treatment enhanced lifespan, stress capacity, antioxidant capacity (T-SOD/CAT/GSH-Px/MDA/ROS), and altered aging-related indicators of lipofuscin accumulation, pharyngeal pump rate, m...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - September 21, 2023 Category: Biochemistry Authors: Miaosi Zhang Zhe Xu Liangyong Shao Jilite Wang Zouyan He Yumei Jiang Ye Zhang Hao Wang Source Type: research
Interactions of human mitochondrial Ferredoxin 1 (Adrenodoxin) by NMR; modulation by cytochrome P450 substrate and by truncation of the C-terminal tail
In this study, protein NMR spectroscopy was used to interrogate the interactions between full-length (2-124) or truncated monomeric (2-108) human Adx and human CYP24A1 (with and without its vitamin-D substrate) as well as interactions with AdR. Here, monomeric Adx induced a similar pattern of peak broadening as that induced by addition of CYP24A1 substrate, consistent with a 1:1 Adx:CYP interaction as the functional complex. Additionally, removal of the C-terminal tail appears to enhance the interaction with AdR, despite removal of some of the AdR contacts in the tail region. This finding was also supported by an NMR compe...
Source: Journal of Inorganic Biochemistry - September 21, 2023 Category: Biochemistry Authors: Natalie Jay Janie E McGlohon D Fernando Estrada Source Type: research
Aqueous chemistry of nalidixic acid and its complexes with biological relevant cations: A combination of potentiometric, UV spectrophotometric, MS and MS/MS study
J Inorg Biochem. 2023 Sep 6;249:112366. doi: 10.1016/j.jinorgbio.2023.112366. Online ahead of print.ABSTRACTNalidixic acid (NAL) is a broad-spectrum antimicrobial widely used for urinary tract infections. As demonstrated, complexation of NAL with Zn2+, Mn2+ and Cu2+ was often used to get new formulations with an enhanced efficiency and potency. Therefore, the elucidation of behavior of NAL in solution and of its interaction with metal cations are crucial to better understand the influence of complexation on NAL efficiency and to find the optimal conditions to propose novel formulations. As a preliminary study, spectrophoto...
Source: Journal of Inorganic Biochemistry - September 21, 2023 Category: Biochemistry Authors: Federica Carnamucio Donatella Aiello Claudia Foti Anna Napoli Ottavia Giuffr è Source Type: research
Establishing acceptable variation between instruments with dry chemistry methodology
Clin Biochem. 2023 Sep 19:110647. doi: 10.1016/j.clinbiochem.2023.110647. Online ahead of print.NO ABSTRACTPMID:37734647 | DOI:10.1016/j.clinbiochem.2023.110647
Source: Clinical Biochemistry - September 21, 2023 Category: Biochemistry Authors: Peter A Kavsak Rahim Ahmed Leesa Lillie Nadia Caruso Source Type: research
Demonstration of equivocal anti-glomerular basement membrane antibody positivity as a non-specific reaction through multiple immunologic assays in a case of pediatric asymptomatic hematuria
CONCLUSION: The commercial assays for anti-GBM antibody can lead to false-positive results. We recommend confirmation of anti-GBM antibody positivity through the use of multiple assays in patients demonstrating an atypical clinical course for anti-GBM disease.PMID:37734646 | DOI:10.1016/j.clinbiochem.2023.110650
Source: Clinical Biochemistry - September 21, 2023 Category: Biochemistry Authors: Masayuki Sato Yuka Nishibata Sakiko Masuda Tsunehisa Nagamori Emi Ishibazawa Yoichiro Yoshida Hironori Takahashi Akihiro Ishizu Satoru Takahashi Source Type: research
Systematic review of type 1 diabetes biomarkers reveals regulation in circulating proteins related to complement, lipid metabolism, and immune response
ConclusionsBiomarkers analyzed in this systematic review highlight alterations in specific biological processes in T1D, including complement, lipid metabolism, and immune response pathways, and may have potential for further use in the clinic as prognostic or diagnostic assays.
Source: Clinical Proteomics - September 21, 2023 Category: Biochemistry Source Type: research
Improving the Enantioselectivity of CHMO Brevi1 for Asymmetric Synthesis of Podophyllotoxin Precursor
In this study, we use a strategy called "focused rational iterative site-specific mutagenesis" (FRISM) at residues ranging of 6 Å from substrate. The mutations by using a restricted set of rationally chosen amino acids allows the formation of a small mutant library. By generating and screening less than 60 variants, we achieved high ee of 96.8%. Coupled with the cofactor regeneration system, 9.3 mM substrate was converted completely in a 100-mL scale reaction. Therefore, our work reveals a promising synthetic method for (R)-β-piperonyl-γ-butyrolactone with the highest enantioselectivity, and provides a new opportunity f...
Source: Chembiochem - September 20, 2023 Category: Biochemistry Authors: Shou-Cheng Huang Yi-Ke Zhang Qiang Geng Qi-Kang Huang Jian-He Xu Yi-Feng Chen Hui-Lei Yu Source Type: research
Phenolic Substitution in Fidaxomicin: A Semisynthetic Approach to Antibiotic Activity Across Species
Chembiochem. 2023 Sep 20:e202300570. doi: 10.1002/cbic.202300570. Online ahead of print.ABSTRACTFidaxomicin (Fdx) is a natural product antibiotic with potent activity against Clostridioides difficile and other Gram-positive bacteria such as Mycobacterium tuberculosis. Only few Fdx derivatives have been synthesized and examined for their biological activity in the 50 years since its discovery. Fdx has a well-studied mechanism of action, namely inhibition of the bacterial RNA polymerase. Yet, the targeted organisms harbor different target protein sequences, which poses a challenge for the rational development of new semisynt...
Source: Chembiochem - September 20, 2023 Category: Biochemistry Authors: Erik Jung Anastassia Kraimps Silvia Dittmann Tizian Griesser Jordan Costafrolaz Yves Mattenberger Simon Jurt Patrick H Viollier Peter Sander Susanne Sievers Karl Gademann Source Type: research
The conversion of UDP-Glc to UDP-Man: In Silico and Biochemical Exploration to Improve the Catalytic Efficiency of CDP-Tyvelose C2-Epimerases
Chembiochem. 2023 Sep 20:e202300549. doi: 10.1002/cbic.202300549. Online ahead of print.ABSTRACTA promiscuous CDP-tyvelose 2-epimerase (TyvE) from Thermodesulfatator atlanticus (TaTyvE) belonging to the Nucleotide Sugar active Short-Chain Dehydrogenase/Reductase superfamily (NS-SDRs) was recently discovered. TaTyvE performs the slow conversion of NDP-glucose (NDP-Glc) to NDP-mannose (NDP-Man). Here, we present the sequence fingerprints that are indicative of the conversion of UDP-Glc to UDP-Man in TyvE-like enzymes based on the heptagonal box motifs. Our data-mining approach led to the identification of 11 additional TyvE-...
Source: Chembiochem - September 20, 2023 Category: Biochemistry Authors: Ulrike Vogel Matthieu Da Costa Carlos Josue Alvarez Quispe Robin Stragier Henk-Jan Joosten Koen Beerens Tom Desmet Source Type: research
Functionalization of bacterial microcompartment shell interior with cysteine containing peptides enhances the iron and cobalt loading capacity
In this study, we present a method for functionalizing recombinant GRM2-type BMC shell lumens with short cysteine-containing sequences and demonstrate that the iron and cobalt loading capacity of such modified shells is markedly increased. These results also imply that a passive flow of cobalt and iron atoms across the BMC shell could be possible.PMID:37728832 | DOI:10.1007/s10534-023-00538-1
Source: Biometals - September 20, 2023 Category: Biochemistry Authors: Gints Kalnins Maris Bertins Arturs Viksna Kaspars Tars Source Type: research
Effects of tetrahydroxy stilbene glycoside derivatives on free radical damage and apoptosis in APP695V717I transgenic mice
Redox Rep. 2023 Dec;28(1):2259246. doi: 10.1080/13510002.2023.2259246. Epub 2023 Sep 20.ABSTRACTMitochondrial dysfunction leading to overproduction of oxygen free radicals is an important event in the development of Alzheimer's disease. Tetrahydroxy stilbene glycoside (TSG) is one of the main effective components of Polygonum multiflorum and has a certain free radical scavenging effect. We synthesized tetrahydroxy stilbene glycoside derivatives (Mito-TSGs) that can cross the mitochondrial membrane and may provide effective protection against Alzheimer's disease. This experiment investigates the protective mechanism of tetr...
Source: Redox Report : communications in free radical research - September 20, 2023 Category: Biochemistry Authors: Jun Qian Yun Li Yanyun Wang Qunying Ye Hongbo Luo Source Type: research
Shuttle between arginine and lysine: influence on cancer immunonutrition
Amino Acids. 2023 Sep 20. doi: 10.1007/s00726-023-03327-9. Online ahead of print.ABSTRACTAmino acids which are essential nutrients for all cell types' survival are also recognised to serve as opportunistic/alternative fuels in cancers auxotrophic for specific amino acids. Accordingly, restriction of amino acids has been utilised as a therapeutic strategy in these cancers. Contrastingly, amino acid deficiencies in cancer are found to greatly impair immune functions, increasing mortality and morbidity rates. Dietary and supplemental amino acids in such conditions have revealed their importance as 'immunonutrients' by modulat...
Source: Amino Acids - September 20, 2023 Category: Biochemistry Authors: R Sindhu M Supreeth Shashanka K Prasad M Thanmaya Source Type: research
