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Therapy: Corticosteroid Therapy

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Total 62 results found since Jan 2013.

Src siRNA prevents corticosteroid-associated osteoporosis in a rabbit model
In an established steroid-associated osteonecrosis (SAON) rabbit model we found recently that blockage Src by siRNA could improve reconstructive repair of osteonecrosis via enhancing osteogenesis and inhibiting bone resorption. The current study investigated if blocking Src was able to prevent steroid-associated osteoporosis (SAOP) in the same SAON animal model. Rabbits were treated with pulsed lipopolysaccharide (LPS) and corticosteroid methylprednisolone (MPS). At 2, 4, 6weeks after induction, Src siRNA, control siRNA and saline were intramedullary injected into proximal femur, respectively.
Source: Bone - November 17, 2015 Category: Orthopaedics Authors: Li-Zhen Zheng, Xin-Luan Wang, Hui-Juan Cao, Shi-Hui Chen, Le Huang, Ling Qin Tags: Original Full Length Article Source Type: research

Role of protein phosphatase 5 (PP5) in mediating corticosteroid insensitivity in airway smooth muscle (ASM) cells in severe asthma
Although the mechanisms mediating corticosteroid resistance in severe asthma are still unknown, recent evidence suggests ASM cells isolated from patients with severe asthma have a decreased insensitivity to dexamethasone (Dex) with respect to the inhibition of chemokine production. Here we investigated whether this corticosteroid insensitivity seen in ASM from severe asthmatics was due to an abnormal GR signalling pathway. We here observed that production of CCL5 and CCL11 by TNF in ASM cells from severe asthmatics (compared to healthy subjects and nonsevere asthma) was not affected by Dex or fluticasone (FP) pretreatment....
Source: European Respiratory Journal - October 30, 2015 Category: Respiratory Medicine Authors: Chachi, L., Abbasian, M., Gavrila, A., Tliba, O., Brightling, C., Amrani, Y. Tags: 3.2 Airway Cell Biology and Immunopathology Source Type: research

LATE-BREAKING ABSTRACT: Inhibition of the ubiquitin-E3-ligase FBXW7 enhances steroid efficacy: New concept for treating steroid insensitivity
Conclusion: Reduction of FBXW7 caused a significant augmentation of functional GR and may have the potential to improve the efficacy of steroids.
Source: European Respiratory Journal - October 30, 2015 Category: Respiratory Medicine Authors: Kaestle, M., Wyatt, D., Nicklin, P. Tags: 5.3 Allergy and Immunology Source Type: research

Esculentoside A Attenuates Allergic Airway Inflammation via Activation of the Nrf-2 Pathway
Conclusions: This is the first study to illustrate that EsA acts as a novel Nrf-2 activator, which modulates the oxidative stress pathway to improve lung injury and ameliorate the development of airway inflammation.Int Arch Allergy Immunol 2015;167:280-290
Source: International Archives of Allergy and Immunology - October 24, 2015 Category: Allergy & Immunology Source Type: research

Pulmonary Delivery of siRNA via Polymeric Vectors as Therapies of Asthma
Asthma is a chronic inflammatory disease. Despite the fact that current therapies, such as the combination of inhaled corticosteroids and β2‐agonists, can control the symptoms of asthma in most patients, there is still an urgent need for an alternative anti‐inflammatory therapy for patients who suffer from severe asthma but lack acceptable response to conventional therapies. Many molecular factors are involved in the inflammatory process in asthma, and thus blocking the function of these factors could efficiently alleviate airway inflammation. RNA interference (RNAi) is often thought to be the answer in the search for...
Source: Archiv der Pharmazie - July 1, 2015 Category: Drugs & Pharmacology Authors: Yuran Xie, Olivia M. Merkel Tags: Minireview Source Type: research

Sphingosine 1-phosphate Increases COX-2 Expression and PGE2 Secretion: Effects on β2-adrenergic Receptor Desensitization.
Abstract Tachyphylaxis of the β2-adrenergic receptor limits the efficacy of bronchodilatory β2-agonists in respiratory disease. Cellular studies in airway smooth muscle (ASM) have shown that inflammatory mediators and infectious stimuli reduce β2-adrenergic responsiveness in a cyclooxygenase 2 (COX-2)-mediated, prostaglandin E2 (PGE2)-dependant manner. Herein we show that sphingosine 1-phosphate (S1P), a bioactive sphingolipid that plays an important role in pathophysiology of asthma, also induces β2-adrenergic receptor desensitization in bronchial ASM cells and exerts hyporesponsiveness to β2-agonist. We tre...
Source: Am J Respir Cell Mol... - June 22, 2015 Category: Respiratory Medicine Authors: Rumzhum NN, Rahman MM, Oliver BG, Ammit AJ Tags: Am J Respir Cell Mol Biol Source Type: research

Increased expression of TLR2 in CD4(+) T cells from SLE patients enhances immune reactivity and promotes IL-17 expression through histone modifications.
This article is protected by copyright. All rights reserved. PMID: 26079624 [PubMed - as supplied by publisher]
Source: European Journal of Immunology - June 15, 2015 Category: Allergy & Immunology Authors: Liu Y, Liao J, Zhao M, Wu H, Yung S, Chan TM, Akihiko Y, Lu Q Tags: Eur J Immunol Source Type: research

Increased expression of TLR2 in CD4+ T cells from SLE patients enhances immune reactivity and promotes IL‐17 expression through histone modifications
This article is protected by copyright. All rights reserved
Source: European Journal of Immunology - June 1, 2015 Category: Allergy & Immunology Authors: Yu Liu, Jieyue Liao, Ming Zhao, Haijing Wu, Susan Yung, Tak Mao Chan, Yoshimura Akihiko, Qianjin Lu Tags: Regular Article Source Type: research

B-cell receptor-guided delivery of peptide-siRNA complex for B-cell lymphoma therapy
Conclusions: Peptide-siRNA complex can be suitable tool for both selective peptide-driven cell targeting and gene silencing. In this setting, the improvement of this strategy is expected to provide a safe and non-invasive approach for the delivery of therapeutic molecules.
Source: Cancer Cell International - May 7, 2015 Category: Cancer & Oncology Authors: Nunzia MigliaccioCamillo PalmieriImmacolata RuggieroGiuseppe FiumeNicola MartucciIris ScalaIleana QuintoGiuseppe ScalaAnnalisa LambertiPaolo Arcari Source Type: research

The Plant Derivative Compound A Inhibits the Production of Corticosteroid-resistant Chemokines by Airway Smooth Muscle Cells.
Abstract Preclinical models of human conditions including asthma showed the therapeutic potential of compound A (CpdA), a dissociated glucocorticoid (GC) receptor (GRα) ligand. Whether CpdA inhibits GC resistance, a central feature of severe asthma, has not been addressed. We investigated whether CpdA modulates cytokine-induced GC resistance in human airway smooth muscle (ASM) cells. Healthy and asthmatic ASM cells were treated with TNFα/IFNγ for 24 hr in the presence or absence of CpdA. ELISA and qPCR assays were used to assess the effect of CpdA on chemokine expression. Activation of GRα by CpdA was assessed...
Source: Am J Respir Cell Mol... - April 21, 2015 Category: Respiratory Medicine Authors: Gavrila A, Chachi L, Tliba O, Brightling C, Amrani Y Tags: Am J Respir Cell Mol Biol Source Type: research

Activation of GX sPLA2 by Furin-like Proprotein Convertases Lipids
In this study, using a FLAG epitope-tagged pro-GX sPLA2 expression construct (FLAG-pro-GX sPLA2), we determined that adrenocorticotropic hormone (ACTH) enhanced FLAG-pro-GX sPLA2 processing and phospholipase activity secreted by Y1 adrenal cells. ACTH increased the expression of furin and PCSK6, but not other members of the PC family, in Y1 cells. Overexpression of furin and PCSK6 in HEK 293 cells significantly enhanced FLAG-pro-GX sPLA2 processing, whereas siRNA-mediated knockdown of both PCs almost completely abolished FLAG-pro-GX sPLA2 processing in Y1 cells. Expression of either furin or PCSK6 enhanced the ability of G...
Source: Journal of Biological Chemistry - March 20, 2015 Category: Chemistry Authors: Layne, J. D., Shridas, P., Webb, N. R. Tags: Cell Biology Source Type: research

Protein tyrosine phosphatase PTPRR regulates PP2A in mononuclear cells
Conclusion: We have demonstrated that reduction of PTPRR down-regulates PP2AC via enhancement of PP2AC-Tyr307 phosphorylation that may lead to corticosteroid insensitivity. This novel mechanism may be a new therapeutic target for restoration of corticosteroid sensitivity in patients with severe asthma.
Source: European Respiratory Journal - December 23, 2014 Category: Respiratory Medicine Authors: Kobayashi, Y., Miller-Larsson, A., Ito, K., Kanda, A., Tomoda, K., Barnes, P. J., Mercado, N. Tags: 3.2 Airway Cell Biology and Immunopathology Source Type: research

Corticosteroid Regulates Epithelial Occludin Expression in Nasal Polyps through a MKP-1-Dependent Pathway
Objective: To evaluate the regulatory effect of corticosteroid on occludin expression in polyp tissues of chronic rhinosinusitis with nasal polyps (CRSwNP) patients and in vitro. Methods: Twenty CRSwNP patients were enrolled and subjected to prednisone (30 mg/day for 14 days). The expression of occludin in polyp tissues was examined before and after treatment using immunohistochemical staining and quantitative reverse transcription polymerase chain reaction. Moreover, the expression of occludin in polyp-derived epithelial cells (PECs) and human bronchial epithelial cells (BECs) was examined using Western blot analysis in t...
Source: ORL - November 19, 2014 Category: ENT & OMF Source Type: research

Enhanced expression of SAM‐pointed domain–containing Ets‐like factor in Chronic rhinosinusitis with nasal polyps
ConclusionsOur findings suggested that SPDEF may be regarded as a promising therapeutic target for modulating mucus hypersecretion in CRSwNP. Level of EvidenceN/A. Laryngoscope, 2014
Source: The Laryngoscope - November 6, 2014 Category: ENT & OMF Authors: Jing Bai, Beiping Miao, Xingmei Wu, Xi Luo, Renqiang Ma, Jia Zhang, Lei Li, Jianbo Shi, Huabin Li Tags: Allergy/Rhinology Source Type: research

Inactivation of histone deacetylase 1 (hdac1) but not hdac2 is required for the glucocorticoid-dependent ccaat/enhancer binding protein α (c/ebpα) expression and preadipocyte differentiation.
In this study, we sought to demonstrate using two different strategies the definite role of HDACl in adipogenesis. By using siRNA-mediated knockdown of HDAC1 and by generating an enzymatically inactive HDAC1D181A by site-directed mutagenesis, we were able to show that HDAC1, but not HDAC2, suppresses GR-potentiated preadipocyte differentiation by decreasing C/ebpα and Pparγ expression levels at the onset of differentiation. Finally, we demonstrate that HDAC1D181A acts as a dominant negative mutant of HDACl during adipogenesis by modulating C/EBPβ transcriptional activity on the C/ebpα promoter. PMID: 25203139 [Pub...
Source: Endocrinology - September 9, 2014 Category: Endocrinology Authors: Kuzmochka C, Abdou HS, Haché RJ, Atlas E Tags: Endocrinology Source Type: research