The Unsettling Ambiguity of Therapeutic Extracellular Vesicles from Mesenchymal Stromal Cells.
PMID: 31805239 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - December 5, 2019 Category: Respiratory Medicine Authors: Mitsialis SA Tags: Am J Respir Cell Mol Biol Source Type: research

Out-of-Tune: Fibroblasts Turn Fibrotic When They Lack a FENDRR.
PMID: 31805248 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - December 5, 2019 Category: Respiratory Medicine Authors: Woeller CF, O'Reilly MA Tags: Am J Respir Cell Mol Biol Source Type: research

December Highlights/Papers by Junior Investigators/NIH News.
Authors: PMID: 31774331 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - November 28, 2019 Category: Respiratory Medicine Tags: Am J Respir Cell Mol Biol Source Type: research

Reply to: Altered Calcium in Ciliary Dysfunction: Potential Role of ER Stress and Ciliophagy.
PMID: 31774332 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - November 28, 2019 Category: Respiratory Medicine Authors: Petit A, Knabe L, Khelloufi K, Jory M, Gras D, Cabon Y, Begg M, Richard S, Massiera G, Chanez P, Vachier I, Bourdin A Tags: Am J Respir Cell Mol Biol Source Type: research

Confounding Effects of Gavage in Mice: Impaired Respiratory Structure and Function.
PMID: 31774333 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - November 28, 2019 Category: Respiratory Medicine Authors: Larcombe AN, Wang KCW, Phan JA, Berry LJ, Noble PB Tags: Am J Respir Cell Mol Biol Source Type: research

Minority Mycobacterium tuberculosis Genotypic Populations as an Indicator of Subsequent Phenotypic Resistance.
PMID: 31774334 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - November 28, 2019 Category: Respiratory Medicine Authors: Engelthaler DM, Streicher EM, Kelley EJ, Allender CJ, Wiggins K, Jimenez D, Lemmer D, Vittinghoff E, Theron G, Sirgel FA, Warren RM, Metcalfe JZ Tags: Am J Respir Cell Mol Biol Source Type: research

Altered Calcium in Ciliary Dysfunction: Potential Role of Endoplasmic Reticulum Stress and Ciliophagy.
PMID: 31774335 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - November 28, 2019 Category: Respiratory Medicine Authors: McAlinden KD, Eapen MS, Ghavami S, Sohal SS, Sharma P Tags: Am J Respir Cell Mol Biol Source Type: research

DNAse to the Rescue! Clearing Mitochondrial DNA May Have NET Benefits in Lung Transplantation.
PMID: 31726015 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - November 14, 2019 Category: Respiratory Medicine Authors: Juvet S, Martinu T Tags: Am J Respir Cell Mol Biol Source Type: research

Effect of Iron Deficiency on a Murine Model of Smoke-Induced Emphysema.
Abstract Smoking is a major risk factor for chronic obstructive pulmonary disease (COPD). Smoking susceptibility is important for the onset and development of COPD. We previously reported an association between serum iron levels and pulmonary function in male smokers. However, the mechanism governing smoking susceptibility in relation to iron deficiency is unclear; this study aimed to elucidate this mechanism. C57BL/6 male mice were fed an iron-deficient or normal diet, and then exposed to cigarette smoke. Bronchoalveolar lavage (BAL), histological analysis, and pulmonary function tests were performed after cigare...
Source: Am J Respir Cell Mol... - November 14, 2019 Category: Respiratory Medicine Authors: Sato K, Inoue S, Igarashi A, Tokairin Y, Yamauchi K, Kimura T, Nishiwaki M, Nemoto T, Nakano H, Sato M, Machida H, Yang S, Minegishi Y, Furuyama K, Watanabe M, Shibata Y Tags: Am J Respir Cell Mol Biol Source Type: research

lncRNA FENDRR Exhibits Anti-Fibrotic Activity in Pulmonary Fibrosis.
CONCLUSIONS: Our data suggest that FENDRR is an anti-fibrotic lncRNA and a potential therapeutic target for pulmonary fibrosis. PMID: 31697569 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - November 7, 2019 Category: Respiratory Medicine Authors: Huang C, Liang Y, Zeng X, Yang X, Xu D, Gou X, Sathiaseelan R, Senavirathna LK, Wang P, Liu L Tags: Am J Respir Cell Mol Biol Source Type: research

HLA-E-Restricted CD8+ T Lymphocytes Efficiently Control Mycobacterium tuberculosis and HIV-1 Co-Infection.
CONCLUSIONS: HLA-E-restricted and Mycobacterium tuberculosis-specific CD8+ T cells in patients with Mycobacterium tuberculosis/HIV-1 co-infection have an exhausted phenotype and fail to expand in vitro in response to antigen stimulation, which can be restored by blocking programmed death-1 pathway using the specific monoclonal antibody Nivolumab. PMID: 31697586 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - November 7, 2019 Category: Respiratory Medicine Authors: La Manna MP, Orlando V, Prezzemolo T, Di Carlo P, Cascio A, Delogu G, Poli G, Dieli F, Caccamo N Tags: Am J Respir Cell Mol Biol Source Type: research

Comment on "Unraveling a Clinical Paradox: Why Does Bronchial Thermoplasty Work in Asthma?"
Comment on "Unraveling a Clinical Paradox: Why Does Bronchial Thermoplasty Work in Asthma?" Am J Respir Cell Mol Biol. 2019 Nov;61(5):660-661 Authors: Brook BS, Chernyavsky IL, Russell RJ, Saunders RM, Brightling CE PMID: 31674824 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - November 1, 2019 Category: Respiratory Medicine Authors: Brook BS, Chernyavsky IL, Russell RJ, Saunders RM, Brightling CE Tags: Am J Respir Cell Mol Biol Source Type: research

Reply: Relevance of the PP2A Pathway in the Molecular Mechanisms of Chronic Obstructive Pulmonary Disease.
PMID: 31674825 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - November 1, 2019 Category: Respiratory Medicine Authors: Nath S, Ohlmeyer M, Salathe MA, Poon J, Baumlin N, Foronjy RF, Geraghty P Tags: Am J Respir Cell Mol Biol Source Type: research

Reply to: Comment on "Unraveling a Clinical Paradox: Why Does Bronchial Thermoplasty Work in Asthma?"
Reply to: Comment on "Unraveling a Clinical Paradox: Why Does Bronchial Thermoplasty Work in Asthma?" Am J Respir Cell Mol Biol. 2019 Nov;61(5):661-663 Authors: Donovan GM, Elliot JG, Green FHY, James AL, Noble PB PMID: 31674826 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - November 1, 2019 Category: Respiratory Medicine Authors: Donovan GM, Elliot JG, Green FHY, James AL, Noble PB Tags: Am J Respir Cell Mol Biol Source Type: research

Immune and Cytokine Dysfunction in Cystic Fibrosis.
PMID: 31674827 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - November 1, 2019 Category: Respiratory Medicine Authors: Leung ST, Khoury O, Barrios C, Ortega VE, Atala A, Murphy SV Tags: Am J Respir Cell Mol Biol Source Type: research

Relevance of the PP2A Pathway in the Molecular Mechanisms of Chronic Obstructive Pulmonary Disease.
Foncillas J PMID: 31674828 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - November 1, 2019 Category: Respiratory Medicine Authors: Cristóbal I, Luque M, Sanz-Alvarez M, Rojo F, García-Foncillas J Tags: Am J Respir Cell Mol Biol Source Type: research

November Highlights/Papers by Junior Investigators/NIH News.
Authors: PMID: 31674829 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - November 1, 2019 Category: Respiratory Medicine Tags: Am J Respir Cell Mol Biol Source Type: research

Methods in Lung Microbiome Research.
Abstract The lung microbiome is associated with host immune response and health outcomes in experimental models and patient cohorts. Lung microbiome research is increasing in volume and scope; however, there are no established guidelines for study design, conduct and reporting of lung microbiome studies. Standardized approaches to yield reliable and reproducible data that can be synthesized across studies, will ultimately improve the scientific rigor and impact of published work and greatly benefit microbiome research. In this review, we identify and address several key elements of microbiome research: conceptual ...
Source: Am J Respir Cell Mol... - October 29, 2019 Category: Respiratory Medicine Authors: Carney SM, Clemente JC, Cox MJ, Dickson RP, Huang YJ, Kitsios GD, Kloepfer KM, Leung JM, LeVan TD, Molyneaux PL, Moore BB, O'Dwyer DN, Segal LN, Garantziotis S, ATS Allergy, Immunology and Inflammation Assembly Working Group on Microbiome in Lung Disease Tags: Am J Respir Cell Mol Biol Source Type: research

Recommended Reading from the Indiana University School of Medicine Division of Pulmonary, Critical Care, Sleep and Occupational Medicine Research Fellows.
PMID: 31633380 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - October 21, 2019 Category: Respiratory Medicine Authors: Chelvanambi S, Hester JM, Sharma S, Lahm T, Frump AL Tags: Am J Respir Cell Mol Biol Source Type: research

"Shocking the System" to Achieve Efficient Gene Targeting in Primary Human Airway Epithelia.
"Shocking the System" to Achieve Efficient Gene Targeting in Primary Human Airway Epithelia. Am J Respir Cell Mol Biol. 2019 Oct 21;: Authors: O'Neal WK, Ribeiro CMP PMID: 31633992 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - October 21, 2019 Category: Respiratory Medicine Authors: O'Neal WK, Ribeiro CMP Tags: Am J Respir Cell Mol Biol Source Type: research

HY-DIN' in the Cilia: Discovery of Central Pair-Related Mutations in Primary Ciliary Dyskinesia.
PMID: 31604022 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - October 11, 2019 Category: Respiratory Medicine Authors: Dutcher SK, Brody SL Tags: Am J Respir Cell Mol Biol Source Type: research

S-Nitrosylation of CHIP Enhances F508Del CFTR Maturation.
Abstract S-Nitrosothiols (SNOs) are endogenous signaling compounds with a diverse spectrum of beneficial airway effects that are both cGMP-dependent and -independent. SNOs are present in healthy human airways, but levels are low in the airways of cystic fibrosis (CF) patients. Here, we evaluated the interactions of SNOs with molecular co-chaperone C-terminus Hsc70 interacting protein (CHIP)- an E3 ubiquitin ligase that targets improperly folded CFTR for degradation. CHIP was expressed in primary human bronchial epithelial and CFBE41o - cells expressing either wild type or F508del CFTR. Confocal microscopy and immu...
Source: Am J Respir Cell Mol... - October 9, 2019 Category: Respiratory Medicine Authors: Zaman K, Knight J, Hussain F, Cao R, Estabrooks SK, Altawallbeh G, Holloway K, Jafri A, Sawczak V, Li Y, Getsy P, Sun F, Raffay T, Cotton C, Brodsky JL, Periasamy A, Lewis SJ, Gaston B Tags: Am J Respir Cell Mol Biol Source Type: research

Efficient RNP-Directed Human Gene Targeting Reveals SPDEF is Required for IL-13-Induced Mucostasis.
Abstract Primary human bronchial epithelial cell (HBEC) cultures are a useful model for studies of lung health and major airway diseases. However, mechanistic studies have been limited by our ability to selectively disrupt specific genes in these cells. Here, we optimize methods for gene targeting in HBECs by direct delivery of single guide RNA (sgRNA) and recombinant Cas9 (rCas9) complexes by electroporation, without a requirement for plasmids, viruses, or antibiotic selection. Variations in the method of delivery, sgRNA and rCas9 concentrations, and sgRNA sequences all had effects on targeting efficiency, allowi...
Source: Am J Respir Cell Mol... - October 9, 2019 Category: Respiratory Medicine Authors: Koh KD, Siddiqui S, Cheng D, Bonser LR, Sun DI, Zlock LT, Finkbeiner WE, Woodruff PG, Erle DJ Tags: Am J Respir Cell Mol Biol Source Type: research

Activation of The Metabolic Master Regulator PPAR γ - A Potential PIOneering Therapy for Pulmonary Arterial Hypertension.
The objective is a data-driven approach to set the preclinical and clinical study record straight. The convincing recent clinical trial data on the lack of significant toxicity in high risk populations justify the timely conduction of clinical studies in order to achieve "repurposing" or "repositioning" of pioglitazone for the treatment of clinical PAH. PMID: 31577451 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - October 2, 2019 Category: Respiratory Medicine Authors: Hansmann G, Calvier L, Risbano MG, Chan SY Tags: Am J Respir Cell Mol Biol Source Type: research

Lipids in COPD: A Target for Future Therapy?
PMID: 31577908 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - October 2, 2019 Category: Respiratory Medicine Authors: Vlahos R Tags: Am J Respir Cell Mol Biol Source Type: research

Muc5b Enhances Murine Honeycomb-like Cyst Formation.
PMID: 31573335 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - October 1, 2019 Category: Respiratory Medicine Authors: Kurche JS, Dobrinskikh E, Hennessy CE, Huber J, Estrella A, Hancock LA, Schwarz MI, Okamoto T, Cool CD, Yang IV, Evans CM, Schwartz DA Tags: Am J Respir Cell Mol Biol Source Type: research

IRE1 Signaling As a Putative Therapeutic Target in Influenza Virus-induced Pneumonia.
r A, Herold S PMID: 31573336 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - October 1, 2019 Category: Respiratory Medicine Authors: Schmoldt C, Vazquez-Armendariz AI, Shalashova I, Selvakumar B, Bremer CM, Peteranderl C, Wasnick R, Witte B, Gattenlöhner S, Fink L, Vadász I, Morty RE, Pleschka S, Seeger W, Günther A, Herold S Tags: Am J Respir Cell Mol Biol Source Type: research

October Highlights/Papers by Junior Investigators/NIH News.
Authors: PMID: 31573337 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - October 1, 2019 Category: Respiratory Medicine Tags: Am J Respir Cell Mol Biol Source Type: research

Stem Cells, Cell Therapies, and Bioengineering in Lung Biology and Diseases 2017. An Official American Thoracic Society Workshop Report.
gner DE Abstract The University of Vermont Larner College of Medicine, in collaboration with the National Heart, Lung, and Blood Institute (NHLBI), the Alpha-1 Foundation, the American Thoracic Society, the Cystic Fibrosis Foundation, the European Respiratory Society, the International Society for Cell & Gene Therapy, and the Pulmonary Fibrosis Foundation, convened a workshop titled "Stem Cells, Cell Therapies, and Bioengineering in Lung Biology and Diseases" from July 24 through 27, 2017, at the University of Vermont, Burlington, Vermont. The conference objectives were to review and discuss current ...
Source: Am J Respir Cell Mol... - October 1, 2019 Category: Respiratory Medicine Authors: Ryan AL, Ikonomou L, Atarod S, Bölükbas DA, Collins J, Freishtat R, Hawkins F, Gilpin SE, Uhl FE, Uriarte JJ, Weiss DJ, Wagner DE Tags: Am J Respir Cell Mol Biol Source Type: research

Effects of Sustained and Intermittent Hypoxia on Human Lung Cancer Cells.
é R, Almendros I PMID: 31573339 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - October 1, 2019 Category: Respiratory Medicine Authors: Marhuenda E, Campillo N, Gabasa M, Martínez-García MA, Campos-Rodríguez F, Gozal D, Navajas D, Alcaraz J, Farré R, Almendros I Tags: Am J Respir Cell Mol Biol Source Type: research

Development of Limb Muscle Dysfunction in COPD - Smoking, Inflammation or Simply Disuse?
PMID: 31532223 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - September 18, 2019 Category: Respiratory Medicine Authors: Iepsen UW, Pedersen BK Tags: Am J Respir Cell Mol Biol Source Type: research

Rhinovirus C15 Induces Airway Hyperresponsiveness Via Calcium Mobilization in Airway Smooth Muscle.
Abstract Rhinovirus (RV) exposure evokes exacerbations of asthma that markedly impact morbidity and mortality worldwide. The mechanisms by which RV induces airway hyperresponsiveness (AHR) or by which specific RV serotypes differentially evoke AHR remain unknown. We posit that RV infection evokes AHR and inflammatory mediator release, which correlate with levels of RV infection. Further, we posit that RVC-induced airway hyperresponsiveness requires paracrine or autocrine mediator release from epithelium that modulates agonist-induced calcium mobilization in human airway smooth muscle (HASM). In these studies we ut...
Source: Am J Respir Cell Mol... - September 18, 2019 Category: Respiratory Medicine Authors: Parikh V, Scala J, Patel R, Corbi C, Lo D, Bochkov YA, Kennedy JL, Kurten RC, Liggett SB, Gern JE, Koziol-White CJ Tags: Am J Respir Cell Mol Biol Source Type: research

NFU1, Iron-Sulfur Biogenesis, and Pulmonary Arterial Hypertension: A (Metabolic) Shift in our Thinking.
PMID: 31526282 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - September 17, 2019 Category: Respiratory Medicine Authors: Culley MK, Perk D, Chan SY Tags: Am J Respir Cell Mol Biol Source Type: research

Embedding of Precision-Cut Lung Slices in Engineered Hydrogel Biomaterials Supports Extended ex vivo Culture.
CM Abstract Maintaining the three-dimensional (3D) architecture and cellular complexity of lung tissue ex vivo can enable elucidation of the cellular and molecular pathways underlying chronic pulmonary diseases. Precision-cut lung slices (PCLS) are one human-lung model with the potential to support critical mechanistic studies and early drug discovery. However, many studies report a short culture period of seven to ten days. Here, we systematically evaluated poly(ethylene glycol)-based hydrogel platforms for the encapsulation of PCLS. We demonstrated the ability to support ex vivo culture of embedded PCLS over 21...
Source: Am J Respir Cell Mol... - September 12, 2019 Category: Respiratory Medicine Authors: Bailey KE, Pino C, Lennon ML, Lyons A, Jacot JG, Lammers SR, Königshoff M, Magin CM Tags: Am J Respir Cell Mol Biol Source Type: research

PI3K Catalytic Subunits α and β Modulate Cell Death and IL-6 Secretion Induced by Talc Particles in Human Lung Carcinoma Cells.
PI3K Catalytic Subunits α and β Modulate Cell Death and IL-6 Secretion Induced by Talc Particles in Human Lung Carcinoma Cells. Am J Respir Cell Mol Biol. 2019 Sep 12;: Authors: Bougen-Zhukov NM, Lee YY, Lee JJ, Lee P, Loo LH Abstract Hydrated magnesium silicate (or "talc" particles) is a sclerosis agent commonly used in the management of malignant pleural effusions (MPE), a common symptom of metastatic diseases including lung cancers. However, the direct effects of talc particles to lung carcinoma cells, which can be found in the MPE fluids from lung cancer patients, are not full...
Source: Am J Respir Cell Mol... - September 12, 2019 Category: Respiratory Medicine Authors: Bougen-Zhukov NM, Lee YY, Lee JJ, Lee P, Loo LH Tags: Am J Respir Cell Mol Biol Source Type: research

PAI-1 Regulation of TGF- β1-induced ATII Cell Senescence, SASP Secretion, and SASP-mediated Activation of Alveolar Macrophages.
In this study, we show that TGF-β1, a most ubiquitous and potent profibrotic cytokine, induces plasminogen activator inhibitor 1 (PAI-1), a cell senescence and fibrosis mediator, and p16 as well as senescence, but not apoptosis, in primary mouse ATII cells. We also found that senescent ATII cells secretes various cytokines and chemokines, including interleukin 4 (IL-4) and 13 (IL-13), which stimulate the expression of genes associated with a pro-fibrotic phenotype in alveolar macrophages. Similar responses were also observed in TGF-β1-treated rat ATII (L2) and rat macrophage NR8383 cells. Deletion of PAI-1 or inh...
Source: Am J Respir Cell Mol... - September 12, 2019 Category: Respiratory Medicine Authors: Rana T, Jiang C, Liu G, Miyata T, Antony V, Thannickal VJ, Liu RM Tags: Am J Respir Cell Mol Biol Source Type: research

Lipid Metabolism: a New Player in the Conundrum of Lung Fibrosis.
PMID: 31499006 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - September 9, 2019 Category: Respiratory Medicine Authors: Summer R, Mora AL Tags: Am J Respir Cell Mol Biol Source Type: research

Lack of IL-1 Receptor Signaling Reduces Spontaneous Airway Eosinophilia in Juvenile Mice with Muco-Obstructive Lung Disease.
In this study, we examined the role of IL-1R signaling in the development of airway eosinophilia and type 2 inflammation in juvenile Scnn1b-Tg mice. We determined effects of genetic deletion of Il1r1 on eosinophil counts, transcript levels of key type 2 cytokines, markers of eosinophil activation and apoptosis, and tissue morphology in lungs of Scnn1b-Tg mice at different time points during neonatal development. Further, we measured endothelial surface expression of intercellular adhesion molecule 1 (ICAM-1), an integrin involved in eosinophil transendothelial migration, and determined effects of eosinophil depletion using...
Source: Am J Respir Cell Mol... - September 9, 2019 Category: Respiratory Medicine Authors: Brown R, Paulsen M, Schmidt S, Schatterny J, Frank A, Hirtz S, Delaney R, Doherty D, Hagner M, Taggart C, Weldon S, Mall MA Tags: Am J Respir Cell Mol Biol Source Type: research

Long Noncoding Transcriptome in Chronic Obstructive Pulmonary Disease.
Abstract Chronic airway inflammation from recurring exposure to noxious environmental stimuli results in a progressive and irreversible airflow limitation and the lung parenchymal damage that characterizes Chronic Obstructive Pulmonary Disease (COPD). The large variability observed in the onset and progression of COPD is primarily driven by complex gene-environment interactions. The transcriptomic and epigenetic memory potential of lung epithelial and innate immune cells drive responses such as mucous hyperreactivity and airway remodeling, tightly regulated by various molecular mechanisms for which several candida...
Source: Am J Respir Cell Mol... - September 5, 2019 Category: Respiratory Medicine Authors: Devadoss D, Long C, Langley RJ, Manevski M, Nair M, Campos MA, Borchert G, Rahman I, Chand HS Tags: Am J Respir Cell Mol Biol Source Type: research

The Role of KCNMB1 and BK Channels in Myofibroblast Differentiation and Pulmonary Fibrosis.
Abstract The differentiation of fibroblasts to myofibroblasts is critical to the development of fibrotic disorders, including idiopathic pulmonary fibrosis (IPF). Previously, we demonstrated that fibroblasts from patients with IPF exhibit changes in DNA methylation across the genome that contribute to a pro-fibrotic phenotype. One of the top differentially methylated genes identified in our previous study was KCNMB1, which codes for the beta subunit of the large conductance potassium channel (BK, MaxiK, KCa1.1). Here, we examine how the expression of KCNMB1 differs in IPF fibroblasts compared to normal cells, and ...
Source: Am J Respir Cell Mol... - September 5, 2019 Category: Respiratory Medicine Authors: Scruggs AM, Grabauskas G, Huang SK Tags: Am J Respir Cell Mol Biol Source Type: research

The Epithelial Expressions of YAP and TAZ are Sequentially Required in Lung Development.
CONCLUSIONS: Our results provide a novel insight into the molecular mechanisms underlying lung development and contributes to a better understanding of the characteristics of TAZ and YAP. PMID: 31486675 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - September 5, 2019 Category: Respiratory Medicine Authors: Isago H, Mitani A, Mikami Y, Horie M, Urushiyama H, Hamamoto R, Terasaki Y, Nagase T Tags: Am J Respir Cell Mol Biol Source Type: research

Influenza A Virus Infection Induces Apical Redistribution of Na+, K+-ATPase in Lung Epithelial Cells In Vitro and In Vivo.
old S PMID: 31469298 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - September 1, 2019 Category: Respiratory Medicine Authors: Peteranderl C, Kuznetsova I, Schulze J, Hardt M, Lecuona E, Sznajder JI, Vadász I, Morty RE, Pleschka S, Wolff T, Herold S Tags: Am J Respir Cell Mol Biol Source Type: research

Peripheral Blood Gene Expression Signatures of Eosinophilic Chronic Obstructive Pulmonary Disease.
PMID: 31469299 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - September 1, 2019 Category: Respiratory Medicine Authors: Yun JH, Chase R, Parker MM, Saferali A, Castaldi PJ, Silverman EK, Hersh CP Tags: Am J Respir Cell Mol Biol Source Type: research

September Highlights/Papers by Junior Investigators/NIH News.
Authors: PMID: 31469300 [PubMed - in process] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - September 1, 2019 Category: Respiratory Medicine Tags: Am J Respir Cell Mol Biol Source Type: research

ABC Transporters: An Overlooked Mechanism of Drug Failure in Our Pre-Clinical Models?
PMID: 31469577 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - August 30, 2019 Category: Respiratory Medicine Authors: Redente EF Tags: Am J Respir Cell Mol Biol Source Type: research

Tissue Resident Alveolar Macrophages Do Not Rely on Glycolysis for LPS-induced Inflammation.
In this study, we show that TR-AMs rely on oxidative phosphorylation to meet their energy demands and maintain extremely low levels of glycolysis under steady-state conditions. Unlike BMDMs, TR-AMs do not experience enhanced glycolysis in response to lipopolysaccharide (LPS), and glycolytic inhibition had no effect on their proinflammatory cytokine production. HIF-1α stabilization promoted glycolysis in TR-AMs and shifted energy production away from oxidative metabolism at baseline, but it was not sufficient for TR-AMs to mount further increases in glycolysis or enhance immune function in response to LPS. Importantly...
Source: Am J Respir Cell Mol... - August 30, 2019 Category: Respiratory Medicine Authors: Woods PS, Kimmig LM, Meliton AY, Sun KA, Tian Y, O'Leary EM, Gökalp GA, Hamanaka RB, Mutlu GM Tags: Am J Respir Cell Mol Biol Source Type: research

A Cation Channel Protects the Stretched Lung.
PMID: 31469582 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - August 30, 2019 Category: Respiratory Medicine Authors: Heise RL, Farkas L Tags: Am J Respir Cell Mol Biol Source Type: research

Bleomycin Induces Drug Efflux in Lungs: A pitfall for Pharmacological Studies of Pulmonary Fibrosis.
CONCLUSION: The study reveals a discrepancy between IPF pathophysiology and the common animal model of lung fibrosis. Bleomycin-induced drug efflux in the murine lungs may present an uncontrolled confounding variable in the preclinical study of IPF drug candidates, and these findings will facilitate disease model validation, enhance new drug discoveries that ultimately improve patient outcomes. PMID: 31419911 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - August 16, 2019 Category: Respiratory Medicine Authors: Park JK, Coffey NJ, Bodine SP, Zawatsky CN, Jay L, Gahl WA, Kunos G, Gochuico BR, Malicdan MCV, Cinar R Tags: Am J Respir Cell Mol Biol Source Type: research

A New Pathway to Airway Relaxation - Targeting the "Other" Cyclase in Asthma.
A New Pathway to Airway Relaxation - Targeting the "Other" Cyclase in Asthma. Am J Respir Cell Mol Biol. 2019 Aug 15;: Authors: Lam M, Bourke JE PMID: 31414885 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - August 15, 2019 Category: Respiratory Medicine Authors: Lam M, Bourke JE Tags: Am J Respir Cell Mol Biol Source Type: research

Kiss of Death: Ring Finger 216 Regulates Toll-like Receptor 8 Stability Through Ubiquitination.
PMID: 31414897 [PubMed - as supplied by publisher] (Source: Am J Respir Cell Mol...)
Source: Am J Respir Cell Mol... - August 15, 2019 Category: Respiratory Medicine Authors: Ghimire L, Jeyaseelan S Tags: Am J Respir Cell Mol Biol Source Type: research