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Specialty: Dermatology
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Total 964 results found since Jan 2013.
922 HIV-1 trans-infection of T-cells via dendritic cells requires endosomal sorting and polarized trafficking to the virological synapse
Dendritic cells (DC) are the 'sentinels' of the immune system and play an essential role in the presentation of antigenic material to T-cells. Human Immunodeficiency Virus 1 (HIV-1) is taken into DC evading degradation and transported to a virological synapse (VS) to promote T-cell infection. DC studies have led to the identification of several restriction factors that aid viral transmission, however how the virus is trafficked through the cell to the VS within DC is still unclear. A high-throughput siRNA method was developed in monocyte derived dendritic cells (MDDC) to identify membrane trafficking proteins involved in t...
Source: Journal of Investigative Dermatology - April 27, 2018 Category: Dermatology Authors: R. Bayliss, J. Wheeldon, S. Caucheteux, C. Niessen, V. Piguet Tags: Innate Immunity, Microbiology, Inflammation Source Type: research
894 Poly(I:C) and GAG inhibit exogenous HSV1 infection in the barrier dysfunctional 3D epidermal model
Skin barrier dysfunction often induces severe herpes simplex virus (HSV) infection that is known as Kaposi's varicelliform eruption (KVE). KVE often occurs on patients with atopic dermatitis or genetic skin disease such as Dariers disease (DD). The mechanism of KVE has not been clearly proved, and the preventive methods have not been found. We previously established a skin barrier dysfunctional 3D model using siRNA of ATP2A2 of which gene mutation is confirmed in DD. This 3D model has dyskeratosis and TEM observation clearly revealed epidermal cleft that is caused by the absence of desmosome at intercellular space in ATP2A...
Source: Journal of Investigative Dermatology - April 27, 2018 Category: Dermatology Authors: E. Sato, K. Hiromatsu, K. Murata, S. Imafuku Tags: Innate Immunity, Microbiology, Inflammation Source Type: research
861 CCN2/Connective tissue growth factor regulates G1 to S phase cell cycle progression in human skin fibroblasts
We report here the unexpected observation that CCN2 functions as a critical regulator of cell cycle progression in primary cultured human skin fibroblasts. Depletion of CCN2 in fibroblasts, by siRNA-mediated knockdown, resulted in near-complete inhibition of proliferation.
Source: Journal of Investigative Dermatology - April 27, 2018 Category: Dermatology Authors: T. Quan, Z. Qin, T. Purohit, Y. Shao, J. Baldassare, J.J. Voorhees, G.J. Fisher Tags: Growth Factors, Cell Adhesion and Matrix Biology Source Type: research
812 Type VII collagen and Nesprin 2, LINCing the basement membrane to altered cell cycle and increased DNA damage
Epidermolysis Bullosa is a severe blistering disease caused by mutations in basement membrane genes and is characterised by severe blistering of the skin and increased predisposition to cancer. To investigate the role of the skin basement membrane, siRNA knockdown of type IV, VII and XVII collagens was performed in primary keratinocytes. Global transcriptomic analysis was carried out using RNA-Seq. Dysregulation of genes involved in DNA damage and cell cycle control was seen in cells with Col7 and Col17 knockdown.
Source: Journal of Investigative Dermatology - April 27, 2018 Category: Dermatology Authors: S. Marsh, M.P. Caley, V. Martins, M. Chen, J. McGrath, M. Barnes, E.A. O'Toole Tags: Genetic Disease, Gene Regulation, and Gene Therapy Source Type: research
761 Topically-delivered gene suppressing nanocontruct targets IL-17RA for psoriasis
Activated interleukin (IL)-23/Th17 signaling is critical for psoriasis pathogenesis, and systemic IL-17 inhibitors are highly effective therapy. However, most patients have mild to moderate disease and no targeted topical therapeutic option. We developed a topically-delivered gene regulating nanoconstruct, comprised of antisense DNA or siRNA in a dense spherical configuration. These spherical nucleic acids or SNAs penetrate the skin barrier to knock down cutaneous gene targets. We generated anti-human and anti-mouse liposomal DNA SNAs targeting the IL-17A receptor (ILRA) and tested their efficacy in reducing IL17RA and imp...
Source: Journal of Investigative Dermatology - April 27, 2018 Category: Dermatology Authors: H. Liu, R. Kang, K. Bagnowski, J. Yu, X. Cheng, S. Radecki, W. Daniel, D. Giljohann, A.S. Paller Tags: Genetic Disease, Gene Regulation, and Gene Therapy Source Type: research
661 Understanding the role of ABCA12 in the pathogenesis of harlequin ichthyosis
Mutations in the ABCA12 gene cause Harlequin Ichthyosis (HI), the most severe ARCI, with a defective skin barrier. The aim of this study was to identify essential pathways involved in HI pathomechanisms, responsible for aberrant epidermal differentiation. We performed RNA-seq on calcium-induced primary keratinocytes with siRNA knockdown of ABCA12 and identified 118 genes significantly down-regulated and 36 genes significantly up-regulated. Functional annotation clustering analysis showed changes in epidermal differentiation, fatty acid metabolism, cytokine and interferon signaling.
Source: Journal of Investigative Dermatology - April 27, 2018 Category: Dermatology Authors: F. Enjalbert, P. Dewan, M.P. Caley, B. Fell, M. Morse, D. Kelsell, A. Enright, E.A. O'Toole Tags: Epidermal Structure and Barrier Function Source Type: research
496 RXI-109 treatment to reduce the formation of hypertrophic dermal scars
RXI-109 is being developed to target and reduce connective tissue growth factor (CTGF), a key regulator of the dermal scarring pathway, to reduce hypertrophic scarring. RXI-109 is a modified small interfering RNA (siRNA) that is designed to enter cells efficiently and reduce CTGF through RNA interference (RNAi). In the US, there are currently no drugs approved to prevent hypertrophic scar formation. A phase 2a clinical study was conducted with RXI-109 to evaluate its impact on the reduction of hypertrophic scar formation after scar revision surgery.
Source: Journal of Investigative Dermatology - April 27, 2018 Category: Dermatology Authors: L. Barefoot, M. Survi, J. Cardia, K. Bulock, G. Dispersyn Tags: Clinical Research: Pathophysiology and Therapeutics Source Type: research
Interleukin-32 promotes detachment and activation of human Langerhans cells in a human skin explant model.
CONCLUSIONS: We propose, for the first time, that IL-32 is a molecular link between KCs and LCs in healthy skin, provoking LC migration from the epidermis to the dermis prior to their migration to the draining lymph nodes.
PMID: 29806155 [PubMed - as supplied by publisher]
Source: The British Journal of Dermatology - May 28, 2018 Category: Dermatology Authors: Gonnet J, Perrin H, Hutton AJ, Boccara D, Bonduelle O, Mimoun M, Atlan M, Soria A, Combadière B Tags: Br J Dermatol Source Type: research
The siRNA ‐mediated downregulation of PD‐1 alone or simultaneously with CTLA‐4 shows enhanced in‐vitro CAR‐T cell functionality for further clinical development towards the potential use in immunotherapy of melanoma
Experimental Dermatology,Volume 0, Issue ja, -Not available-.
Source: Experimental Dermatology - April 28, 2018 Category: Dermatology Authors: Bianca Simon
,
Dennis C Harrer
,
Beatrice Schuler ‐Thurner
,
Niels Schaft
,
Gerold Schuler
,
Jan Dörrie
,
Ugur Uslu Source Type: research
The siRNA ‐mediated downregulation of PD‐1 alone or simultaneously with CTLA‐4 shows enhanced in vitro CAR‐T‐cell functionality for further clinical development towards the potential use in immunotherapy of melanoma
Experimental Dermatology,Volume 27, Issue 7, Page 769-778, July 2018.
Source: Experimental Dermatology - June 28, 2018 Category: Dermatology Authors: Bianca
Simon
,
Dennis C.
Harrer
,
Beatrice
Schuler ‐Thurner
,
Niels
Schaft
,
Gerold
Schuler
,
Jan
Dörrie
,
Ugur
Uslu Source Type: research
New onset psoriasis in a patient with chronic inflammatory demyelinating polyneuropathy treated with Rituximab.
Authors: Russo F, Vispi M, Bocci S, Mancini V, Sirna R, Giannini F, Pellegrino M
Abstract
PMID: 30350559 [PubMed - as supplied by publisher]
Source: Giornale Italiano di Dermatologia e Venereologia - October 25, 2018 Category: Dermatology Tags: G Ital Dermatol Venereol Source Type: research
Tuscan consensus on the use of UVBnb phototherapy in the treatment of psoriasis.
Authors: Russo F, Vispi M, Sirna R, Mancini V, Bagnoni G, Bartoli L, Bellini M, Brandini L, Caproni M, Castelli A, Cecchi R, Cuccia A, Fimiani M, Grazzini M, Marsili F, Mazzatenta C, Niccoli MC, Panduri S, Peccianti C, Pecenco S, Pimpinelli N, Prignano F, Ricceri F, Romanelli M, Rossari S, Taviti F, Volpi W, Pellegrino M
Abstract
Psoriasis (PSO) is traditionally defined as an immune-mediated, inflammatory dermatological disease characterized by a chronic-relapsing course and associated with multifactorial inheritance (genetic predisposition and influence of various environmental factors). Considered until recently ...
Source: Giornale Italiano di Dermatologia e Venereologia - October 31, 2018 Category: Dermatology Tags: G Ital Dermatol Venereol Source Type: research
477 Identification of host cell factors involved HIV-1 trans-infection in dendritic cells
Dendritic cells (DC) are antigen presenting cells responsible for processing and presenting antigenic material to T-cells leading to the initiation of an adaptive immune response. DC are one of the first cells to encounter Human Immunodeficiency Virus-1 (HIV-1) transmitted via the sub-mucosal route. DC act as a ‘Trojan horse’ delivering the virus to an infectious synapse (IS), subsequently infecting target CD4+ T-cells. How HIV-1 is trafficked in DC to the IS is still unclear, therefore we conducted a siRNA screen in monocyte derived dendritic cells targeting 140 membrane trafficking genes with the aim of identifying h...
Source: Journal of Investigative Dermatology - April 19, 2019 Category: Dermatology Authors: R.J. Bayliss, J. Wheeldon, S. Caucheteux, C.M. Niessen, V. Piguet Tags: Innate Immunity, Microbiology, and Microbiome Source Type: research
375 The chromatin architectural protein CTCF regulates expression of pro-inflammatory cytokines in the skin cells
Increased evidence suggests that alterations in chromatin structure result in aberrant pro-inflammatory cytokines expression that leads to prolonged inflammatory responses. CCCTC-binding factor (CTCF) is a transcriptional repressor that insulates the expression of neighbouring genes and is involved in chromatin interactions between distal and proximal gene regulatory elements. However, the role of CTCF in the control of pro-inflammatory cytokines expression in the skin cells remains unknown. Here, we show that CTCF is expressed in the nuclei of normal human epidermal keratinocytes (NHEK) and dermal fibroblasts (DF), while ...
Source: Journal of Investigative Dermatology - April 19, 2019 Category: Dermatology Authors: M. Boboljova, I. Asamaowei, V. Botchkarev, M. Fessing, A.N. Mardaryev Tags: Genetic Disease, Gene Regulation, and Gene Therapy Source Type: research
Cutaneous wound healing in diabetic mice is improved by topical mineralocorticoid receptor blockade
Skin ulcers resulting from impaired wound healing are a serious complication of diabetes. Unresolved inflammation, associated with the dysregulation of both the phenotype and function of macrophages, is involved in the poor healing of diabetic wounds. Here, we report that topical pharmacological inhibition of the mineralocorticoid receptor (MR) by canrenoate or MR siRNA can resolve inflammation to improve delayed skin wound healing in diabetic mouse models; importantly, wounds from normal mice are unaffected.
Source: Journal of Investigative Dermatology - July 2, 2019 Category: Dermatology Authors: Van Tuan Nguyen, Nicolette Farman, Roberto Palacios-Ramirez, Maria Sbeih, Francine Behar-Cohen, S élim Aractingi, Frederic Jaisser Tags: Original Article Source Type: research
