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Drug: Medroxyprogesterone

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Total 120 results found since Jan 2013.

Characterization of GAB1 expression over the menstrual cycle in women with and without Polycystic Ovarian Syndrome provides a new insight into its pathophysiology.
Conclusions: GAB1 mRNA expression is positively modulated by E2. Endometrial GAB1 protein and mRNA expression are reduced in women with PCOS, suggesting that the endometrium of PCOS women have a defect in insulin signaling due to GAB1 down-regulation. PMID: 25144631 [PubMed - as supplied by publisher]
Source: The Journal of Clinical Endocrinology and Metabolism - August 21, 2014 Category: Endocrinology Authors: Roemer KL, Young SL, Savaris RF Tags: J Clin Endocrinol Metab Source Type: research

GR-mediated Regulation of Cytokine Genes by MPA Signal Transduction
This study shows that P4 and MPA significantly increase the expression of the IL-12p40 and IL-12p35 genes, whereas IL-10 gene expression is suppressed in a dose-dependent manner. Moreover, these effects were abrogated when reducing the glucocorticoid receptor (GR) levels with siRNA. Using a combination of chromatin immunoprecipitation (ChIP), siRNA, and re-ChIP assays, we show that recruitment of the P4- and MPA-bound GR to the IL-12p40 promoter requires CCAAT enhancer-binding protein (C/EBP)-β and nuclear factor κB (NFκB), although recruitment to the IL-10 promoter requires signal transducer and activator of transcript...
Source: Journal of Biological Chemistry - November 6, 2014 Category: Chemistry Authors: Louw-du Toit, R., Hapgood, J. P., Africander, D. Tags: Gene Regulation Source Type: research

Removal of REV-ERBα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells.
This study focused on Ptgs2, which is essential for decidualization, as a putative clock-controlled gene, and aimed to reveal the functions of clock genes in relation to Ptgs2 during decidualization. We compared the transcript levels of clock genes in the rat uterus on days 4.5 (D4.5) and 6.5 of pregnancy. The transcript levels of clock genes (Per2, Bmal1, Rorα and Rev-erbα) had decreased at implantation sites on day 6.5 (D6.5e) compared to those on D4.5, while Ptgs2 transcripts had increased on D6.5e. Similar observations of REV-ERBα and PTGS2 were also obtained in the endometrium on D6.5e by immunohistochemistry. In t...
Source: Physiological Reviews - February 3, 2015 Category: Physiology Authors: Isayama K, Zhao L, Chen H, Yamauchi N, Shigeyoshi Y, Hashimoto S, Hattori MA Tags: Am J Physiol Endocrinol Metab Source Type: research

Removal of Rev-erb{alpha} inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells
This study focused on Ptgs2, which is essential for decidualization, as a putative clock-controlled gene, and aimed to reveal the functions of clock genes in relation to Ptgs2 during decidualization. We compared the transcript levels of clock genes in the rat uterus on days 4.5 (D4.5) and 6.5 of pregnancy. The transcript levels of clock genes (Per2, Bmal1, Rorα, and Rev-erbα) had decreased at implantation sites on day 6.5 (D6.5e) compared with those on D4.5, whereas Ptgs2 transcripts had increased on D6.5e. Similar observations of Rev-erbα and Ptgs2 were also obtained in the endometrium on D6.5e by immuno...
Source: AJP: Endocrinology and Metabolism - April 15, 2015 Category: Endocrinology Authors: Isayama, K., Zhao, L., Chen, H., Yamauchi, N., Shigeyoshi, Y., Hashimoto, S., Hattori, M.-a. Tags: Articles Source Type: research

GSE200312 Glucocorticoid recptor knockdown in primary amnion epithelial cells treated with MPA and TNFa
Contributors : Sarah J Cunningham ; Terrence K Allen ; Tim E Reddy ; Alejandro BarreraSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo analyze the effect of glucocorticoids on primary amnion epthelial cells, we harvested tissue samples from 10 healthy ceseaean section patients. We knocked down the glucocorticoid receptor with an siRNA or used a control siRNA. We treated samples with medroxyprogesterone acetate. Half the cells we subjected to a TNFa challenge
Source: GEO: Gene Expression Omnibus - April 9, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

The PRB-dependent FOXO1/IGFBP-1 axis is essential for progestin to inhibit endometrial epithelial growth
Abstract: Progestin inhibits the growth of normal and cancerous endometria via the progesterone receptor (PR), but the distinct functions and signalings of PR subtypes have not been fully understood. The aim of the present study was to dissect the key pathways of progestin to inhibit endometrial epithelial growth. Immortalized endometrial epithelial cells (EM-E6/E7/TERT) with stable PRA or PRB expression were established and used for the experiments. In vitro growth inhibition by progestin was mainly observed in EM-E6/E7/TERT cells with PRB rather than those with PRA. RT-PCR assay confirmed that FOXO1, a key gene for proge...
Source: Cancer Letters - May 13, 2013 Category: Cancer & Oncology Authors: Mitsuhiro Nakamura, Masahiro Takakura, Reina Fujii, Yoshiko Maida, Yukiko Bono, Yasunari Mizumoto, Xian Zhang, Tohru Kiyono, Satoru Kyo Tags: Research Articles Source Type: research

Inhibitory role of REV-ERBα in the expression of bone morphogenetic protein gene family in rat uterus endometrium stromal cells.
Abstract Uterus circadian rhythms have been implicated in the gestation processes of mammals through entraining of the clock proteins to numerous downstream genes. Bone morphogenetic proteins (BMPs), having clock-controlled regulatory sites in their gene promoters, are expressed in the uterus during decidualization, but the regulation of the Bmp gene expression is poorly understood. The present study was designed to dissect the physiological roles of the uterus oscillators in the Bmp expression using the uterus endometrial stromal cells (UESCs) isolated from Per2-dLuc transgenic rats on day 4.50 of gestation. The ...
Source: Physiological Reviews - January 14, 2015 Category: Physiology Authors: Tasaki H, Zhao L, Isayama K, Chen H, Yamauchi N, Shigeyoshi Y, Hashimoto S, Hattori MA Tags: Am J Physiol Cell Physiol Source Type: research

Progesterone increases nitric oxide synthesis in human vascular endothelial cells through activation of membrane progesterone receptor alpha (mPRα).
Abstract Progesterone exerts beneficial effects on the human cardiovascular system by inducing rapid increases in nitric oxide (NO) production in vascular endothelial cells, but the receptors mediating these nongenomic progesterone actions remain unclear. Using human umbilical vein endothelial cells (HUVECs) as a model, we show that progesterone binds to plasma membranes of HUVECs with the characteristics of membrane progesterone receptors (mPRs). The selective mPR agonist, Org OD 02-0 had high binding affinity for the progesterone receptor on HUVEC membranes, whereas nuclear PR (nPR) agonists R5020 and medroxypro...
Source: American Journal of Physiology. Endocrinology and Metabolism - March 24, 2015 Category: Physiology Authors: Pang Y, Dong J, Thomas P Tags: Am J Physiol Endocrinol Metab Source Type: research

Inhibitory role of REV-ERB{alpha} in the expression of bone morphogenetic protein gene family in rat uterus endometrium stromal cells
Uterus circadian rhythms have been implicated in the gestation processes of mammals through entraining of the clock proteins to numerous downstream genes. Bone morphogenetic proteins (BMPs), having clock-controlled regulatory sites in their gene promoters, are expressed in the uterus during decidualization, but the regulation of the Bmp gene expression is poorly understood. The present study was designed to dissect the physiological roles of the uterus oscillators in the Bmp expression using the uterus endometrial stromal cells (UESCs) isolated from Per2-dLuc transgenic rats on day 4.5 of gestation. The in vitro decidualiz...
Source: AJP: Cell Physiology - April 1, 2015 Category: Cytology Authors: Tasaki, H., Zhao, L., Isayama, K., Chen, H., Yamauchi, N., Shigeyoshi, Y., Hashimoto, S., Hattori, M.-a. Tags: CALL FOR PAPERS Source Type: research

Progesterone increases nitric oxide synthesis in human vascular endothelial cells through activation of membrane progesterone receptor-{alpha}
Progesterone exerts beneficial effects on the human cardiovascular system by inducing rapid increases in nitric oxide (NO) production in vascular endothelial cells, but the receptors mediating these nongenomic progesterone actions remain unclear. Using human umbilical vein endothelial cells (HUVECs) as a model, we show that progesterone binds to plasma membranes of HUVECs with the characteristics of membrane progesterone receptors (mPRs). The selective mPR agonist Org OD 02-0 had high binding affinity for the progesterone receptor on HUVEC membranes, whereas nuclear PR (nPR) agonists R5020 and medroxyprogesterone acetate d...
Source: AJP: Endocrinology and Metabolism - May 15, 2015 Category: Endocrinology Authors: Pang, Y., Dong, J., Thomas, P. Tags: Articles Source Type: research

Abstract 37: Role and regulation of CYP24A1 in endometrial cancer
The cytochrome P450 enzyme, 24-hydroxylase, encoded by CYP24A1 is critical for the catabolism of 1,25(OH)2D3 (calcitriol). The unbalanced high levels of CYP24A1 seem to be a determinant of calcitriol resistance in tumors. We have previously shown that progesterone enhances calcitriol antitumor activity by upregulating vitamin D receptor expression and promoting apoptosis in endometrial cancer cells. In the present study, we evaluated CYP24A1 protein expression in normal and endometrial tumor tissues, assessed the effect of progesterone and calcitriol on CYP24A1 and its spliced variant expression in endometrial cancer cell ...
Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Bokhari, A. A., Lee, L. R., Dewayne, R., Hamilton, C. A., Maxwell, G. L., Rodriguez, G. C., Syed, V. Tags: Molecular and Cellular Biology Source Type: research

Panobinostat Enhances Growth Suppressive Effects of Progestin on Endometrial Carcinoma by Increasing Progesterone Receptor and Mitogen-Inducible Gene-6
AbstractAlthough progestin has been used to treat endometrial hyperplasia and endometrial carcinoma (EC), its therapeutic efficacy is limited. In order to improve this, the underlining mechanisms of the effects of progestin need to be elucidated in more detail. In the present study, we examined the involvement of mitogen-inducible gene-6 (MIG6), a negative regulator of the EGF receptor, in the progestin-mediated growth suppression of endometrial epithelia. The immunohistochemical expression of MIG6 was elevated in the early to mid-secretory phases of normal endometrium and also with endometrial hyperplasia after medroxypro...
Source: Hormones and Cancer - May 17, 2017 Category: Cancer & Oncology Source Type: research

NLRP7 contributes to in vitro decidualization of endometrial stromal cells.
CONCLUSIONS: NLRP7 was upregulated and translocated to the nucleus of the endometrial stromal cells in an in vitro decidualization model. Overexpressed NLRP7 promoted the IGFBP-1 expression and PR reporter activation. IGFBP-1 expression decreased with the knockdown of NLRP7. Therefore, we suggest that NLRP7 contributes to in vitro decidualization of endometrial stromal cells. PMID: 28810880 [PubMed - in process]
Source: Reproductive Biology - August 15, 2017 Category: Reproduction Medicine Authors: Huang JY, Yu PH, Li YC, Kuo PL Tags: Reprod Biol Endocrinol Source Type: research

Gelsemine and koumine, principal active ingredients of Gelsemium, exhibit mechanical antiallodynia via spinal glycine receptor activation-induced allopregnanolone biosynthesis.
Abstract Gelsemine, the principal active alkaloid from Gelsemium sempervirens Ait., and koumine, the most dominant alkaloids from Gelsemium elegans Benth., produced antinociception in a variety of rodent models of painful hypersensitivity. The present study explored the molecular mechanisms underlying gelsemine- and koumine-induced mechanical antiallodynia in neuropathic pain. The radioligand binding and displacement assays indicated that gelsemine and koumine, like glycine, were reversible and orthosteric agonists of glycine receptors with full efficacy and probably acted on same binding site as the glycine recep...
Source: Biochemical Pharmacology - January 19, 2019 Category: Drugs & Pharmacology Authors: Shoaib RM, Zhang JY, Mao XF, Wang YX Tags: Biochem Pharmacol Source Type: research

Early growth response 1 transcriptionally primes the human endometrial stromal cell for decidualization
Publication date: Available online 31 January 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Maria M. Szwarc, Lan Hai, William E. Gibbons, Qianxing Mo, Rainer B. Lanz, Francesco J. DeMayo, John P. LydonAbstractMouse studies support a role for endometrial early growth response 1 (EGR1) in uterine receptivity and decidualization, which are processes controlled by estrogen and progesterone. However, the importance of this transcription factor in similar cellular processes in human is unclear. Analysis of clinical samples indicate that endometrial EGR1 levels are decreased in the endometrium of...
Source: The Journal of Steroid Biochemistry and Molecular Biology - February 1, 2019 Category: Biochemistry Source Type: research