Filtered By:
Drug: Doxycycline
This page shows you your search results in order of date. This is page number 2.
Order by Relevance | Date
Total 35 results found since Jan 2013.
Lentiviral ‑mediated inducible silencing of TLR4 attenuates neuropathic pain in a rat model of chronic constriction injury.
In conclusion, TLR4 may serve a significant role in neuropathy and the results of the present study provide an inducible lentivirus‑mediated siRNA against TLR4 that may serve as a potential novel strategy to be applied in gene therapy for NP in the future.
PMID: 30365084 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - October 27, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Metformin Promotes 2-Deoxy-2- 18 FFluoro-D-Glucose Uptake in Hepatocellular Carcinoma Cells Through FoxO1-Mediated Downregulation of Glucose-6-Phosphatase
ConclusionsWe propose that treatment of HCC cells with Met may be a useful strategy for improving the efficacy of [18F]FDG as a tracer for PET/CT imaging of HCC tumors in patients.
Source: Molecular Imaging and Biology - December 18, 2017 Category: Molecular Biology Source Type: research
Lactate-mediated mitoribosomal defects impair mitochondrial oxidative phosphorylation and promote hepatoma cell invasiveness Gene Regulation
Impaired mitochondrial oxidative phosphorylation (OXPHOS) capacity, accompanied by enhanced glycolysis, is a key metabolic feature of cancer cells, but its underlying mechanism remains unclear. Previously, we reported that human hepatoma cells that harbor OXPHOS defects exhibit high tumor cell invasiveness via elevated claudin-1 (CLN1). In the present study, we show that OXPHOS-defective hepatoma cells (SNU354 and SNU423 cell lines) exhibit reduced expression of mitochondrial ribosomal protein L13 (MRPL13), a mitochondrial ribosome (mitoribosome) subunit, suggesting a ribosomal defect. Specific inhibition of mitoribosomal ...
Source: Journal of Biological Chemistry - December 8, 2017 Category: Chemistry Authors: Young-Kyoung Lee, Jin J. Lim, Un-woo Jeoun, Seongki Min, Eun-beom Lee, So Mee Kwon, Changhan Lee, Gyesoon Yoon Tags: Molecular Bases of Disease Source Type: research
Cx32 suppresses extrinsic apoptosis in human cervical cancer cells via the NF ‑κB signalling pathway.
In conclusion, Cx32 suppressed TNFα /TRAIL-induced extrinsic apoptosis by upregulating the NF‑κB signalling pathway. This study demonstrates a novel mechanism for Cx32's anti-apoptotic effect and provides a reasonable explanation for the pro-tumour effect of Cx32 in human CaCx cells.
PMID: 28902345 [PubMed - in process]
Source: International Journal of Oncology - September 15, 2017 Category: Cancer & Oncology Authors: Lai Y, Fan L, Zhao Y, Ge H, Feng X, Wang Q, Zhang X, Peng Y, Wang X, Tao L Tags: Int J Oncol Source Type: research
Cx32 inhibits TNF α-induced extrinsic apoptosis with and without EGFR suppression.
Cx32 inhibits TNFα-induced extrinsic apoptosis with and without EGFR suppression.
Oncol Rep. 2017 Sep 07;:
Authors: Lai Y, Tao L, Zhao Y, Zhang X, Sun X, Wang Q, Xu C
Abstract
Tumor necrosis factor α (TNFα) and TNF-related apoptosis-inducing ligand (TRAIL) can trigger the extrinsic apoptosis pathway. Our previous study indicated that connexin32 (Cx32) inhibited streptonigrin-induced intrinsic apoptosis via the epidermal growth factor receptor (EGFR) pathway. However, whether Cx32 can exert effects on the extrinsic apoptosis pathway through EGFR signaling remains unclear. In the present study, we inv...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
Differential Expression of OATP1B3 Mediates Unconjugated Testosterone Influx
This study was undertaken to ascertain the androgen uptake kinetics, functional, and clinical relevance of de novo expression of the steroid hormone transporter OATP1B3 (SLCO1B3). Experiments testing the cellular uptake of androgens suggest that testosterone is an excellent substrate of OATP1B3 (Km = 23.2 μmol/L; Vmax = 321.6 pmol/mg/minute), and cells expressing a doxycycline-inducible SLCO1B3 construct had greater uptake of a clinically relevant concentration of 3H-testosterone (50 nmol/L; 1.6-fold, P = 0.0027). When compared with Slco1b2 (–/–) mice, Slco1b2 (–/–)/hSLCO1B3 knockins had greater ...
Source: Molecular Cancer Research - August 1, 2017 Category: Cancer & Oncology Authors: Sissung, T. M., Ley, A. M., Strope, J. D., McCrea, E. M., Beedie, S., Peer, C. J., Shukla, S., van Velkinburgh, J., Reece, K., Troutman, S., Campbell, T., Fernandez, E., Huang, P., Smith, J., Thakkar, N., Venzon, D. J., Brenner, S., Lee, W., Merino, M., L Tags: Signal Transduction Source Type: research
IL-24 modulates the high mobility group (HMG) A1/miR222 /AKT signaling in lung cancer cells.
Authors: Panneerselvam J, Srivastava A, Muralidharan R, Wang Q, Zheng W, Zhao L, Chen A, Zhao YD, Munshi A, Ramesh R
Abstract
Interleukin (IL)-24, a novel tumor suppressor/cytokine exhibits antitumor activity against a broad-spectrum of human cancer cells. In a recent study, we showed that IL-24 inhibited AKT in lung cancer cells. However, the molecular mechanism of AKT inhibition by IL-24 remains elusive.The high mobility group (HMG) A1 a member of the non-histone chromosomal proteins and commonly referred to as architectural transcription factor, regulates transcription of various genes involved in cell growth an...
Source: Oncotarget - September 10, 2016 Category: Cancer & Oncology Tags: Oncotarget Source Type: research
Cellular uptake of lead in the blood-cerebrospinal fluid barrier: Novel roles of Connexin 43 hemichannel and its down-regulations via Erk phosphorylation.
This study was designed to investigate the roles of Cx43 in Pb uptake in the epithelial cells. Autometallography was used to outline Pb's subcellular location, and the characteristics of Pb transport into CP cells, including concentration- and time-dependence were analyzed by atomic absorption spectroscopy. Knockdown/overexpression of Cx43 with transient siRNA/plasmids transfections before Pb exposure diminished/increased the Pb accumulation. In the Z310 cell-based doxycycline-inducible Cx43 expression cell line (iZCx43), doxycycline induced a significant increase (3-fold) in Pb uptake, corresponding to the increased Cx43 ...
Source: Toxicology and Applied Pharmacology - February 26, 2016 Category: Toxicology Authors: Song H, Zheng G, Liu Y, Shen XF, Zhao ZH, Aschner M, Luo WJ, Chen JY Tags: Toxicol Appl Pharmacol Source Type: research
The activation of KSHV lytic cycle blocks autophagy in PEL cells.
This study confirms that autophagy is activated concomitantly with KSHV lytic cycle induction, and that autophagy inhibition by BECN1 knockdown reduces viral lytic gene expression. In addition, we extend previous observations and show that autophagy is blocked at late steps, during viral replication. This is indicated by the lack of colocalization of autophagosomes and lysosomes and by the LC3-II level that does not increase in the presence of bafilomycin A1 in primary effusion lymphoma (PEL) cells induced to enter the lytic cycle, either by TPA/sodium butyrate (BC3 and BCBL1) or by doxycycline (TRExBCBL1-Rta). The autopha...
Source: Autophagy - September 21, 2015 Category: Cytology Authors: Granato M, Santarelli R, Filardi M, Gonnella R, Farina A, Torrisi MR, Faggioni A, Cirone M Tags: Autophagy Source Type: research
Increased Signaling via Adenosine A1 Receptors, Sleep Deprivation, Imipramine, and Ketamine Inhibit Depressive-like Behavior via Induction of Homer1a
Publication date: 5 August 2015 Source:Neuron, Volume 87, Issue 3 Author(s): Tsvetan Serchov, Hans-Willi Clement, Martin K. Schwarz, Felice Iasevoli, Dilip K. Tosh, Marco Idzko, Kenneth A. Jacobson, Andrea de Bartolomeis, Claus Normann, Knut Biber, Dietrich van Calker Major depressive disorder is among the most commonly diagnosed disabling mental diseases. Several non-pharmacological treatments of depression upregulate adenosine concentration and/or adenosine A1 receptors (A1R) in the brain. To test whether enhanced A1R signaling mediates antidepressant effects, we generated a transgenic mouse with enhanc...
Source: Neuron - August 6, 2015 Category: Neuroscience Source Type: research
Abstract 1140: PI3K/Akt inhibition decreases oxygen consumption In tumor cells by phosphorylating and inactivating pyruvate dehydrogenase PDH E1{alpha} subunit
The PI3K/mTOR pathway plays a central role in coupling metabolic processes to the cellular proliferative state. Pharmacologic inhibitors of the PI3K/mTOR pathway or genetic inhibition of Akt/PI3K decreased the oxygen consumption rate (OCR) in transformed cell lines in vitro by 30-40%. Pharmacologic inhibition of this pathway increased phosphorylation of the E1α subunit of the pyruvate dehydrogenase (PDH) complex on Ser293, an inhibitory modification of this critical gatekeeper of mitochondrial respiration. Expressing wild type PTEN in a doxycycline-inducible manner in a glioblastoma cell line with mutant PTEN led to an in...
Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Cerniglia, G. J., Day, S., Gallagher-Colombo, S. M., Daurio, N., Tuttle, S., Busch, T. M., , Lin, A., Esipova, T. V., Vinogradov, S. A., Koumenis, C., Maity, A. Tags: Molecular and Cellular Biology Source Type: research
Abstract 1713: IL-24 inhibits lung cancer cell migration and invasion by disrupting the SDF-1/CXCR4 signaling axis
ConclusionsOur study results demonstrate that IL-24 inhibits lung tumor cell migration and invasion by disrupting the SDF-1/CXCR4 signaling pathway and exhibits enhanced anti-metastatic activity when combined with CXCR4 inhibitors.Citation Format: Janani Panneerselvam, Jiankang Jin, Manish Shanker, Jason Lauderdale, Jonathan Bates, Qi Wang, Daniel Zhao, Stephen Archibald, Timothy Hubin, Rajagopal Ramesh. IL-24 inhibits lung cancer cell migration and invasion by disrupting the SDF-1/CXCR4 signaling axis. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; ...
Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Panneerselvam, J., Jin, J., Shanker, M., Lauderdale, J., Bates, J., Wang, Q., Zhao, D., Archibald, S., Hubin, T., Ramesh, R. Tags: Experimental and Molecular Therapeutics Source Type: research
Abstract B05: PI3K/mTOR pathway-dependent regulation of oxygen metabolism via pyruvate dehydrogenase (PDH)-E1alpha phosphorylation
The PI3K/mTOR pathway plays a central role in coupling metabolic processes to the cellular proliferative state. In the current study we show that pharmacological inhibition of this pathway leads to a decrease in hypoxia within SQ20B human head and neck cancer xenografts. The mechanism underlying the effect appears in part to be due to reduced tumor cell oxygen consumption induced by the drug. Pharmacologic inhibitors of the PI3K/mTOR pathway or genetic inhibition of Akt/PI3K decreased the oxygen consumption rate (OCR) in transformed cell lines in vitro by 30-40%. Pharmacologic inhibition of this pathway increased phosphory...
Source: Molecular Cancer Therapeutics - July 6, 2015 Category: Cancer & Oncology Authors: Cerniglia, G., Dey, S., Gallagher-Colombo, S. M., Daurio, N., Tuttle, S., Busch, T. M., Lin, A., Esipova, T. V., Vinogradov, S., Koumenis, C., Maity, A. Tags: Downstream Effectors Underlying Cancer Progression: Poster Presentations - Proffered Abstracts Source Type: research
Phosphorylation of interleukin (IL)-24 is required for mediating its anti-cancer activity.
In this study we conducted molecular studies to determine whether IL-24 phosphorylation is important for IL-24-mediated anti-cancer activity.Human H1299 lung tumor cell line that was stably transfected with a doxycycline (DOX)-inducible (Tet-on) plasmid vector carrying the cDNA of IL-24-wild-type (IL-24wt) or IL-24 with all five phosphorylation sites replaced (IL-24mt) was used in the present study. Inhibition of tumor cell proliferation, cell migration and invasion, and induction of G2/M cell cycle arrest was observed in DOX-induced IL-24wt-expressing cells but not in IL-24mt-expressing cells. Secretion of IL-24mt protein...
Source: Oncotarget - May 28, 2015 Category: Cancer & Oncology Tags: Oncotarget Source Type: research
IL-24 Inhibits Lung Cancer Cell Migration and Invasion by Disrupting The SDF-1/CXCR4 Signaling Axis
Conclusions IL-24 disrupts the SDF-1/CXCR4 signaling pathway and inhibits lung tumor cell migration and invasion. Additionally, IL-24, when combined with CXCR4 inhibitors exhibited enhanced anti-metastatic activity and is an attractive therapeutic strategy for lung metastasis.
Source: PLoS One - March 16, 2015 Category: Biomedical Science Authors: Janani Panneerselvam et al. Source Type: research
