Monitoring Immune Cell Function Through Optical Imaging: a Review Highlighting Transgenic Mouse Models
AbstractTransgenic mouse models have facilitated research of human diseases and validation of therapeutic approaches. Inclusion of optical reporter genes (fluorescent or bioluminescent genes) in the targeting vectors used to develop such models makesin vivo imaging of cellular and molecular events possible, from the microscale to the macroscale. In particular, transgenic mouse models expressing optical reporter genes allowed accurately distinguishing immune cell types from traffickingin vivo using intravital microscopy or whole-body optical imaging. Besides lineage tracing and trafficking of different subsets of immune cel...
Source: Molecular Imaging and Biology - November 4, 2021 Category: Molecular Biology Source Type: research

Correction to: Longitudinal FRET Imaging of Glucose and Lactate Dynamics and Response to Therapy in Breast Cancer Cells
(Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - November 2, 2021 Category: Molecular Biology Source Type: research

A Pilot Study of Dynamic 18F-DCFPyL PET/CT Imaging of Prostate Adenocarcinoma in High-Risk Primary Prostate Cancer Patients
ConclusionHigh-risk primary prostate cancer is readily identified on dynamic and static, delayed,18F-DCFPyL PET images. The tumor-to-background ratio increases over time, with optimal18F-DCFPyL PET/CT imaging at 120 min p.i. for evaluation of prostate cancer, but not necessarily ideal for clinical application. Primary prostate cancer demonstrates different uptake kinetics in comparison to BPH and normal prostate tissue. The 15-fold difference inKi between prostate cancer and non-cancer (BPH and normal) tissues translates to an ability to distinguish prostate cancer from normal tissue at time points as early as 5 to 10 min ...
Source: Molecular Imaging and Biology - November 1, 2021 Category: Molecular Biology Source Type: research

Prognostic Value of Fluorine-19 MRI Oximetry Monitoring in cancer
AbstractHypoxia is a key prognostic indicator in most solid tumors, as it is correlated to tumor angiogenesis, metastasis, recurrence, and response to therapy. Accurate measurement and mapping of tumor oxygenation profile and changes upon intervention could facilitate disease progression assessment and assist in treatment planning. Currently, no gold standard exists for non-invasive spatiotemporal measurement of hypoxia. Magnetic resonance imaging (MRI) represents an attractive option as it is a clinically available and non-ionizing imaging modality. Specifically, perfluorocarbon (PFC) beacons can be externally introduced ...
Source: Molecular Imaging and Biology - October 27, 2021 Category: Molecular Biology Source Type: research

PET Imaging of VLA-4 in a New BRAFV600E Mouse Model of Melanoma
AbstractPurposeDespite unprecedented responses to immune checkpoint inhibitors and targeted therapy in melanoma, a major subset of patients progresses and have few effective salvage options. We have previously demonstrated robust, selective uptake of the peptidomimetic LLP2A labeled with Cu-64 ([64Cu]-LLP2A) for positron emission tomography (PET) imaging in subcutaneous and metastatic models of B16F10 murine melanoma. LLP2A binds with high affinity to very late antigen-4 (VLA-4, integrin α4β1), a transmembrane protein overexpressed in melanoma and other cancers that facilitates tumor growth and metastasis. Yet B...
Source: Molecular Imaging and Biology - October 25, 2021 Category: Molecular Biology Source Type: research

19F MRI Imaging Strategies to Reduce Isoflurane Artifacts in In Vivo Images
AbstractPurposeIsoflurane (ISO) is the most commonly used preclinical inhalation anesthetic. This is a problem in19F MRI of fluorine contrast agents, as ISO signals cause artifacts that interfere with unambiguous image interpretation and quantification; the two most attractive properties of heteronuclear MRI. We aimed to avoid these artifacts using MRI strategies that can be applied by any pre-clinical researcher.ProceduresThree strategies to avoid ISO chemical shift displacement artifacts (CSDA) in19F MRI are described and demonstrated with measurements of19F-containing agents in phantoms andin vivo (n = 3 f...
Source: Molecular Imaging and Biology - October 20, 2021 Category: Molecular Biology Source Type: research

Visions by WIMIN: BIPOC Representation Matters
This article focuses on the key component of representati on in the United States (U.S.), highlighting our vision for a solution for the so-called “leaky pipeline” for BIPOC in science, technology, engineering, and mathematic with action items to end it. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - October 15, 2021 Category: Molecular Biology Source Type: research

CXCR4-Directed PET/CT with [68Ga]Pentixafor in Central Nervous System Lymphoma: A Comparison with [18F]FDG PET/CT
ConclusionsCXCR4-directed PET/CT using [68 Ga]Pentixafor, with excellent tumor-to-background contrast, might be a more promising agent for the detection of lesions in CNSL patients than [18F]FDG PET/CT. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - October 14, 2021 Category: Molecular Biology Source Type: research

Improved Discrimination of Tumors with Low and Heterogeneous EGFR Expression in Fluorescence-Guided Surgery Through Paired-Agent Protocols
ConclusionThe quantitative receptor delineation of PAI promises to improve the surgical therapeutic index of cancer resection in a clinically relevant timeline. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - October 14, 2021 Category: Molecular Biology Source Type: research

Side-by-Side Comparison of uPAR-Targeting Optical Imaging Antibodies and Antibody Fragments for Fluorescence-Guided Surgery of Solid Tumors
ConclusionsIn a side-by-side comparison, FGS with uPAR-targeting antibody fragments compared with the parent IgG resulted in earlier tumor visualization at the expense of peak fluorescence intensity. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - October 12, 2021 Category: Molecular Biology Source Type: research

Identification of a Suitable Untargeted Agent for the Clinical Translation of ABY-029 Paired-Agent Imaging in Fluorescence-Guided Surgery
ConclusionPAI is a highly promising methodology for increasing detection of tumors in fluorescence-guided surgery. Although not yet clinically approved, IRDye 680LT demonstrates promise as an untargeted agent when paired with ABY-029. The clinical translation of PAI to maximize tumor excision, while minimizing normal tissue removal, could improve both patient survival and life quality. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - October 12, 2021 Category: Molecular Biology Source Type: research

Application of a Flexible PET Scanner Combined with 3  T MRI Using Non-local Means Reconstruction: Qualitative and Quantitative Comparison with Whole-Body PET/CT
AbstractPurposeFlexible positron emission tomography (fxPET) employing a non-local means reconstruction algorithm was designed to fit existing magnetic resonance imaging (MRI) systems. We aimed to compare the qualitative and quantitative performance of fxPET among fxPET with MR-based attenuation correction (MRAC), fxPET with CT-based attenuation correction (CTAC) using CT as a part of WB PET/CT, and whole-body (WB) PET/CT.ProceduresSixteen patients with suspected head and neck cancer underwent 2-deoxy-2-[18F]fluoro-d-glucose WB PET/CT scans, followed by fxPET and 3  T MRI scans. Phantom data were compared among the th...
Source: Molecular Imaging and Biology - October 12, 2021 Category: Molecular Biology Source Type: research

In vivo Imaging of Cannabinoid Type 2 Receptors: Functional and Structural Alterations in Mouse Model of Cerebral Ischemia by PET and MRI
ConclusionsThis study revealed an increased [18F]RoSMA-18-d6 measure of CB2R and a reduced [18F]FDG measure of CMRglc in the ischemic striatum of tMCAO mice at subacute stage. [18F]RoSMA-18-d6 might be a promising PET tracer for detecting CB2R alterations in animal models of neuroinflammation without neuronal loss. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - October 12, 2021 Category: Molecular Biology Source Type: research

Clinical Translation of Neutrophil Imaging and Its Role in Cancer
AbstractNeutrophils are the first line of defense against pathogens and abnormal cells. They regulate many biological processes such as infections and inflammation. Increasing evidence demonstrated a role for neutrophils in cancer, where different subpopulations have been found to possess both pro- or anti-tumorigenic functions in the tumor microenvironment. In this review, we discuss the phenotypic and functional diversity of neutrophils in cancer, their prognostic significance, and therapeutic relevance in human and preclinical models. Molecular imaging methods are increasingly used to probe neutrophil biologyin vivo, as...
Source: Molecular Imaging and Biology - October 12, 2021 Category: Molecular Biology Source Type: research

Comparison of Invasive and Non-invasive Estimation of [11C]PBR28 Binding in Non-human Primates
ConclusionsPopulation-based input functions scaled with a single blood sample might be a useful alternative to using AIF to compute [11C]PBR28 binding in healthy NHPs or animals with comparable metabolism and overall perform better than pseudo-reference regions approaches. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - October 7, 2021 Category: Molecular Biology Source Type: research

Sodium Pump Na  + /K + ATPase Subunit α1-Targeted Positron Emission Tomography Imaging of Hepatocellular Carcinoma in Mouse Models
ConclusionsNKA α1 may serve as a suitable diagnostic biomarker for HCC.18F-ALF-NOTA-S3 shows great potential for PET imaging of HCC. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - October 7, 2021 Category: Molecular Biology Source Type: research

Image-to-Images Translation for Multiple Virtual Histological Staining of Unlabeled Human Carotid Atherosclerotic Tissue
ConclusionsThe proposed approach allows for the virtual staining of unlabeled human carotid plaque tissue images with multiple types of stains. In addition, it identifies the histopathological features of atherosclerotic plaques in the same tissue sample, which could facilitate the development of personalized prevention and other interventional treatments for carotid atherosclerosis. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - October 7, 2021 Category: Molecular Biology Source Type: research

PET/MRI and PET/CT Radiomics in Primary Cervical Cancer: A Pilot Study on the Correlation of Pelvic PET, MRI, and CT Derived Image Features
AbstractPurposeTo evaluate the correlation of radiomic features in pelvic [2-deoxy-2-18F]fluoro-d-glucose positron emission tomography/magnetic resonance imaging and computed tomography ([18F]FDG PET/MRI and [18F]FDG PET/CT) in patients with primary cervical cancer (CCa).ProceduresNineteen patients with histologically confirmed primary squamous cell carcinoma of the cervix underwent same-day [18F]FDG PET/MRI and PET/CT. Two nuclear medicine physicians performed a consensus reading in random order. Free-hand regions of interest covering the primary cervical tumors were drawn on PET, contrast-enhanced pelvic CT, and pelvic M...
Source: Molecular Imaging and Biology - October 7, 2021 Category: Molecular Biology Source Type: research

Amyloid- β PET Classification on Cognitive Aging Stages Using the Centiloid Scale
ConclusionSuperAgers exhibited a similar A β load to AC and MC, differing in cognitive performance. Independently of cut-point used (10 CL, 19 CL, or 30 CL), three SA individuals were classified as Aβ positive, showing the duality between the individual’s clinics and the biological definition of Alzheimer’s. Different cut-points lead t o Aβ positivity classification mismatch in individuals, and an extra care is needed for individuals who have a CL value between 10 and 30 CL. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - October 5, 2021 Category: Molecular Biology Source Type: research

Longitudinal FRET Imaging of Glucose and Lactate Dynamics and Response to Therapy in Breast Cancer Cells
This study transfected FRET reporters quantifying glucose and lactate concentration into breast cancer cell lines to study nutrient dynamics and response to therapy.ProceduresTwo FRET reporters, one assaying glucose concentration and one assaying lactate concentration, were stably transfected into the MDA-MB-231 breast cancer cell line. Correlation between FRET measurements and ligand concentration were measured using a confocal microscope and a cell imaging plate reader. Longitudinal changes in glucose and lactate concentration were measured in response to treatment with CoCl2, cytochalasin B, and phloretin which, respect...
Source: Molecular Imaging and Biology - October 5, 2021 Category: Molecular Biology Source Type: research

Correction to: Paramagnetic Fluorinated Nanoemulsions for In Vivo F-19 MRI
(Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - September 30, 2021 Category: Molecular Biology Source Type: research

In Vivo MRI Tracking of Tumor Vaccination and Antigen Presentation by Dendritic Cells
AbstractCancer vaccination using tumor antigen-primed dendritic cells (DCs) was introduced in the clinic some 25  years ago, but the overall outcome has not lived up to initial expectations. There are to the complexity of the immune response, there are many factors that determine the efficacy of DC therapy. These include accurate administration of DCs in the target tissue site without unwanted cell dispersion /backflow, sufficient numbers of tumor antigen-primed DCs homing to lymph nodes (LNs), and proper timing of immunoadjuvant administration. To address these uncertainties, proton (1H) and fluorine (19F) magnetic r...
Source: Molecular Imaging and Biology - September 28, 2021 Category: Molecular Biology Source Type: research

Potential of Gold Nanoparticles for Noninvasive Imaging and Therapy for Vascular Inflammation
In this study, we aimed to assess the viability of macrophages incubated with GNPs after near-infrared (NIR) laser light exposure and to evaluate the utility of intravenously injected GNPs forin vivo imaging of vascular inflammation in mice using micro-CT.ProceduresMouse macrophage cells (RAW 264.7) were incubated with GNPs and assessed for GNP cellular uptake and cell viability before and after exposure to NIR laser light. Forin vivo imaging, macrophage-rich atherosclerotic lesions were induced by carotid ligation in hyperlipidemic and diabetic FVB mice (n = 9). Abdominal aortic aneurysms (AAAs) were created...
Source: Molecular Imaging and Biology - September 27, 2021 Category: Molecular Biology Source Type: research

ImmunoPET: harnessing antibodies for imaging immune cells
AbstractDramatic, but uneven, progress in the development of immunotherapies for cancer has created a need for better diagnostic technologies including innovative non-invasive imaging approaches. This review discusses challenges and opportunities for molecular imaging in immuno-oncology and focuses on the unique role that antibodies can fill. ImmunoPET has been implemented for detection of immune cell subsets, activation and inhibitory biomarkers, tracking adoptively transferred cellular therapeutics, and many additional applications in preclinical models. Parallel progress in radionuclide availability and infrastructure s...
Source: Molecular Imaging and Biology - September 22, 2021 Category: Molecular Biology Source Type: research

Development and Validation of a PET/SPECT Radiopharmaceutical in Oncology
AbstractIn oncology, biomarker research aimed to provide insights on cancer biology via positron emission tomography (PET) and single photon emission tomography (SPECT) imaging has seen an incredible growth in the past two decades. Despite the increased number of publications on PET/SPECT radiopharmaceuticals, the field lacked standardization of in vitro and in vivo parameters necessary for the characterization of any radiotracer. Through the efforts of the World Molecular Imaging Society Education Committee, this white paper lays down validation studies that are essential to chemically and biologically characterize new ra...
Source: Molecular Imaging and Biology - September 20, 2021 Category: Molecular Biology Source Type: research

Direct Comparison of [18F]F-DPA with [18F]DPA-714 and [11C]PBR28 for Neuroinflammation Imaging in the same Alzheimer ’s Disease Model Mice and Healthy Controls
In this study we compared the recently developed TSPO tracer [18F]F-DPA, with [18F]DPA-714 and [11C]PBR28 by performingin vivo PET imaging on the same Alzheimer ’s disease mouse model APP/PS1-21 (TG) and wild-type (WT) mice with all three radiotracers.ProceduresTo compare the radiotracer uptake, percentage of injected dose/mL (%ID/mL), standardized uptake value ratios to cerebellum (SUVRCB), and voxel-wise analyses were performed.ResultsThe peak uptake of [18F]F-DPA was higher than 4.3% ID/mL, while [18F]DPA-714 reached just over 3% ID/mL, and [11C]PBR28 was over 4% ID/mL in only one brain region in the WT mice. The ...
Source: Molecular Imaging and Biology - September 20, 2021 Category: Molecular Biology Source Type: research

Incremental Impact of [68  Ga]Ga-PSMA-11 PET/CT in Primary N and M Staging of Prostate Cancer Prior to Curative-Intent Surgery: a Prospective Clinical Trial in Comparison with mpMRI
Conclusion[68 Ga]Ga-PSMA-11 PET/CT shows high diagnostic performance for N and M staging of patients with intermediate- and high-risk prostate cancer and seems to be superior to pelvic mpMRI in the detection of locoregional lymph node metastases. A significant correlation was found between SUVmax of the intrapr ostatic index lesion and risk stratification based on tPSA level and GS. The results of this study emphasize again on the role of metabolic molecular imaging using specific tracers in selected patients, leading to tailored therapy approach. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - September 14, 2021 Category: Molecular Biology Source Type: research

Positivity Rate of [18F]Fluciclovine PET/CT in Patients with Suspected Prostate Cancer Recurrence at PSA Levels Below 1 ng/mL
AbstractPurposeEarly and precise localization of recurrent prostate cancer lesions after local therapy facilitates optimal disease management. Here, we present results from a single-center study to evaluate the utility of [18F]fluciclovine PET/CT to localize prostate cancer recurrence in patients with PSA
Source: Molecular Imaging and Biology - September 3, 2021 Category: Molecular Biology Source Type: research

Synthesis and Biological Evaluation of [18F]FECNT-d4 as a Novel PET Agent for Dopamine Transporter Imaging
ConclusionThe deuterated compound [18F]FECNT-d4 may serve as a promising PET imaging agent to assess DAT-related disorders. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - September 2, 2021 Category: Molecular Biology Source Type: research

Monitoring PSMA Responses to ADT in Prostate Cancer Patient-Derived Xenograft Mouse Models Using [18F]DCFPyL PET Imaging
AbstractPurposePSMA overexpression has been associated with aggressive prostate cancer (PCa). However, PSMA PET imaging has revealed highly variable changes in PSMA expression in response to ADT treatment ranging from increases to moderate decreases. To better understand these PSMA responses and potential relationship to progressive PCa, the PET imaging agent, [18F]DCFPyL, was used to assess changes in PSMA expression in response to ADT using genomically characterized LuCaP patient-derived xenograft mouse models (LuCaP-PDXs) which were found to be sensitive to ADT (LuCaP73 and LuCaP136;CS) or resistant (LuCaP167;CR).Method...
Source: Molecular Imaging and Biology - September 2, 2021 Category: Molecular Biology Source Type: research

Comparison of the [18F]-FDG and [18F]-FLT PET Tracers in the Evaluation of the Preclinical Proton Therapy Response in Hepatocellular Carcinoma
AbstractPurposeThe main objective of the present study was to compare the 2-deoxy-2-[18F]fluoro-D-glucose ([18F]-FDG) and 3 ′-[18F]fluoro-3 ′-deoxythymidine ([18F]-FLT) PET imaging biomarkers for the longitudinal follow-up of small animal proton therapy studies in the context of hepatocellular carcinoma (HCC).ProceduresSK-HEP-1 cells were injected into NMRI nude mice to mimic human HCC. The behavior of [18F]-FDG and [18F]-FLT tumor uptake was evaluated after proton therapy procedures. The proton single-fraction doses were 5, 10, and 20 Gy, with a dose rate of 10 Gy/min. The experimental protocol consisted of 8 ...
Source: Molecular Imaging and Biology - September 2, 2021 Category: Molecular Biology Source Type: research

Desorption Electrospray Ionization Mass Spectrometry Imaging of Cimbi-36, a 5-HT2A Receptor Agonist, with Direct Comparison to Autoradiography and Positron Emission Tomography
ConclusionsDESI imaging from anin vivo dosing experiment showed distribution of the PET tracer remarkably similar to what was obtained by autoradiography of anin vitro incubation experiment, indicating that the obtained results represent actual binding to certain receptors in the brain. DESI-MSI is suggested as a cost-effective screening tool, which does not rely on labelling of compounds. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - September 2, 2021 Category: Molecular Biology Source Type: research

124I-Iodo-DPA-713 Positron Emission Tomography in a Hamster Model of SARS-CoV-2 Infection
AbstractPurposeMolecular imaging has provided unparalleled opportunities to monitor disease processes, although tools for evaluating infection remain limited. Coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is mediated by lung injury that we sought to model. Activated macrophages/phagocytes have an important role in lung injury, which is responsible for subsequent respiratory failure and death. We performed pulmonary PET/CT with124I-iodo-DPA-713, a low-molecular-weight pyrazolopyrimidine ligand selectively trapped by activated macrophages cells, to evaluate the local im...
Source: Molecular Imaging and Biology - August 23, 2021 Category: Molecular Biology Source Type: research

Optimized Doxycycline-Inducible Gene Expression System for Genetic Programming of Tumor-Targeting Bacteria
ConclusionsOverall, doxycycline-induced co-expression of two proteins at similar expression levels, we exploited bioluminescence reporter proteins with preclinical but no clinical utility. Future validation with clinically compatible reporter systems, for example, suitable for radionuclide imaging, is necessary to develop this system further towards potential clinical application. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - August 17, 2021 Category: Molecular Biology Source Type: research

Correction to: Positron Emission Tomographic Imaging of Tumor Cell Death Using Zirconium-89-Labeled APOMAB ® Following Cisplatin Chemotherapy in Lung and Ovarian Cancer Xenograft Models
(Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - August 16, 2021 Category: Molecular Biology Source Type: research

GlucoCEST MRI for the Evaluation Response to Chemotherapeutic and Metabolic Treatments in a Murine Triple-Negative Breast Cancer: A Comparison with[18F]F-FDG-PET
ConclusionsMetabolic changes during doxorubicin treatment can be predicted by glucoCEST imaging that appears more sensitive than [18F]F-FDG-PET in reporting on therapeutic response. These findings support the view that glucoCEST may be a sensitive technique for monitoring metabolic response, but future studies are needed to explore the accuracy of this approach in other tumor types and treatments. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - August 12, 2021 Category: Molecular Biology Source Type: research

Translational Development of a Zr-89-Labeled Inhibitor of Prostate-specific Membrane Antigen for PET Imaging in Prostate Cancer
We present here a Zr-89-labeled inhibitor of prostate-specific membrane antigen (PSMA) as a complement to the already established F-18- or Ga-68-ligands.ProceduresThe precursor PSMA-DFO (ABX) was used for Zr-89-labeling. This is not an antibody, but a peptide analogue of the precursor for the production of [177Lu]Lu-PSMA-617. The ligand [89Zr]Zr-PSMA-DFO was compared with [68Ga]Ga-PSMA-11 and [18F]F-JK-PSMA-7in vitro by determination of theKd value, cellular uptake, internalization in LNCaP cells, biodistribution studies with LNCaP prostate tumor xenografts in mice, andin vivo by small-animal PET imaging in LNCaP tumor mou...
Source: Molecular Imaging and Biology - August 9, 2021 Category: Molecular Biology Source Type: research

Correction to: High-Yielding Radiosynthesis of [68  Ga]Ga-PSMA-11 Using a Low-Cost Microfluidic Device
(Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - August 2, 2021 Category: Molecular Biology Source Type: research

Letter to the Editor: “Integration of PET/CT Radiomics and Semantic Features for Differentiation Between Active Pulmonary Tuberculosis and Lung Cancer”
(Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - July 28, 2021 Category: Molecular Biology Source Type: research

Molecular Imaging Reveals a High Degree of Cross-Seeding of Spontaneous Metastases in a Novel Mouse Model of Synchronous Bilateral Breast Cancer
ConclusionsA high degree of metastatic cross-seeding of cancer cells derived from bilateral tumors may contribute to faster metastatic growth and intratumoral heterogeneity. We posit that our work will help understand treatment resistance and optimal planning of SBBC treatment. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - July 26, 2021 Category: Molecular Biology Source Type: research

Reply to Letter to Editor RE: “Integration of PET/CT Radiomics and Semantic Features for Differentiation Between Active Pulmonary Tuberculosis and Lung Cancer”
(Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - July 26, 2021 Category: Molecular Biology Source Type: research

Correction to: Cross-Modality Imaging of Murine Tumor Vasculature —a Feasibility Study
(Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - July 23, 2021 Category: Molecular Biology Source Type: research

Examining the Feasibility of Quantifying Receptor Availability Using Cross-Modality Paired-Agent Imaging
AbstractPurposeThe ability to noninvasively quantify receptor availability (RA) in solid tumors is an aspirational goal of molecular imaging, often challenged by the influence of non-specific accumulation of the contrast agent. Paired-agent imaging (PAI) techniques aim to compensate for this effect by imaging the kinetics of a targeted agent and an untargeted isotype, often simultaneously, and comparing the kinetics of the two agents to estimate RA. This is usually accomplished using two spectrally distinct fluorescent agents, limiting the technique to superficial tissues and/or preclinical applications. Applying the appro...
Source: Molecular Imaging and Biology - July 20, 2021 Category: Molecular Biology Source Type: research

Molecular Imaging, How Close to Clinical Precision Medicine in Lung, Brain, Prostate and Breast Cancers
AbstractPrecision medicine is playing a pivotal role in strategies of cancer therapy. Unlike conventional one-size-fits-all chemotherapy or radiotherapy modalities, precision medicine could customize an individual treatment plan for cancer patients to acquire superior efficacy, while minimizing side effects. Precision medicine in cancer therapy relies on precise and timely tumor biological information. Traditional tissue biopsies, however, are often inadequate in meeting this requirement due to cancer heterogeneity, poor tolerance, and invasiveness. Molecular imaging could detect tumor biology characterization in a noninva...
Source: Molecular Imaging and Biology - July 16, 2021 Category: Molecular Biology Source Type: research

Synthesis and Initial Characterization of a Reversible, Selective 18F-Labeled Radiotracer for Human Butyrylcholinesterase
ConclusionsThe reversibly acting, selective radiotracer is synthetically easily accessible and retains promising activity and binding potential onhBChE. Radiosynthesis with18F labeling of tosylates was feasible in a reasonable time frame and good radiochemical yield. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - July 14, 2021 Category: Molecular Biology Source Type: research

Spectrum of [18F]FDG-PET/CT Findings in Benign Lymph Node Pathology
AbstractDiffuse lymphadenopathy has a long differential diagnosis that includes both malignant and benign causes. As part of the lymphadenopathy work-up, many patients undergo [18F]FDG-PET/CT for purposes of ruling out malignancy. FDG-avid lymph nodes, however, are not specific for malignancy. This review will illustrate the spectrum of nodal findings on FDG-PET/CT with correlation to other cross-sectional imaging and clinical history in patients with representative infectious, inflammatory, and benign lymphoproliferative disorders. These findings are important for the nuclear medicine radiologist to understand, as they ca...
Source: Molecular Imaging and Biology - July 14, 2021 Category: Molecular Biology Source Type: research

Effect of Shortening the Scan Duration on Quantitative Accuracy of [18F]Flortaucipir Studies
ConclusionsThis study demonstrated that [18F]flortaucipir PET scan can be acquired with sufficient quantitative accuracy using only 50  min of dual-time-window scanning time. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - July 14, 2021 Category: Molecular Biology Source Type: research

Synthesis and Characterization of [18F]JNJ-46356479 as the First 18F-Labeled PET Imaging Ligand for Metabotropic Glutamate Receptor 2
Conclusions[18F]8 readily crosses the blood-brain barrier and demonstrates fast and reversible kinetics both in rodents and in a non-human primate. Further investigation of [18F]8 on its binding specificity would warrant translational study in human. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - July 14, 2021 Category: Molecular Biology Source Type: research

The Clinical Utility of 18F-Fluciclovine PET/CT in Biochemically Recurrent Prostate Cancer: an Academic Center Experience Post FDA Approval
AbstractPurposeTo evaluate the diagnostic performance and clinical utility of18F-fluciclovine PET/CT in patients with biochemical recurrence (BCR) of prostate cancer (PC).Methods18F-Fluciclovine scans of 165 consecutive men with BCR after primary definitive treatment with prostatectomy (n = 102) or radiotherapy (n = 63) were retrospectively evaluated. Seventy patients had concurrent imaging with at least one other conventional modality (CT (n = 31), MRI (n = 31), or bone scan (n = 26)). Findings from18F-fluciclovine PET were compared with those from conv...
Source: Molecular Imaging and Biology - July 14, 2021 Category: Molecular Biology Source Type: research

Evaluation of Optimal Post-Injection Timing of Hypoxic Imaging with 18F-Fluoromisonidazole-PET/CT
ConclusionsWe observed a high correlation between the quantitative values at 2  h and 4 h. When using a combined high-quality PET/CT, the total examination time for FMISO-PET can be shortened by skipping the 4-h scan. (Source: Molecular Imaging and Biology)
Source: Molecular Imaging and Biology - July 14, 2021 Category: Molecular Biology Source Type: research