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Total 90 results found since Jan 2013.

Designing potential siRNA molecules for silencing the gene of the nucleocapsid protein of Nipah virus: A computational investigation
Infect Genet Evol. 2022 May 27:105310. doi: 10.1016/j.meegid.2022.105310. Online ahead of print.ABSTRACTNipah virus (NiV), a zoonotic virus, engenders severe infections with noticeable complications and deaths in humans and animals. Since its emergence, it is frightening that this virus has been causing regular outbreaks in various countries, particularly Bangladesh, India, and Malaysia. Unfortunately, no efficient vaccine or drug is available to combat this baneful virus. It employs the nucleocapsid protein in its genetic material encapsidation, which is a crucial for viral replication. The small interfering RNAs (siRNAs)...
Source: Infection, Genetics and Evolution - May 31, 2022 Category: Genetics & Stem Cells Authors: A M U B Mahfuz Md Arif Khan Emran Hossain Sajib Anamika Deb Shafi Mahmud Mahmudul Hasan Otun Saha Ariful Islam M Mizanur Rahaman Source Type: research

Endothelial Cell-Derived TGF- β Promotes Epithelial-Mesenchymal Transition via CD133 in HBx-Infected Hepatoma Cells
Conclusion: The study indicates that secretory factors like TGF-β from neighboring endothelial cells may enhance expression of CD133 and impart an aggressive EMT phenotype to HBx-infected hepatoma cells in HBV induced HCC. Introduction Hepatocellular Carcinoma (HCC) is one of the most common cancer worldwide, representing approximately 4% of all malignancies (1). It has been estimated that more than 50% of HCC cases in the world are associated with hepatitis B virus (HBV) (2). HBV is a partially double stranded DNA virus belonging to the Hepadnavirus family. The HBV genome is 3.2 kb in size and contains fou...
Source: Frontiers in Oncology - April 23, 2019 Category: Cancer & Oncology Source Type: research

Inhibition of coxsackievirus infection in cardiomyocytes by small dsRNA targeting its cognate coxsackievirus adenovirus receptor.
Abstract Background & objectives: Coxsackievirus B (CVB), a member of human Enterovirus group, is the most common cause of viral myocarditis. Coxsackievirus adenovirus receptor (CAR) is identified as a key determinant for the entry of CVB in the target cells. Thus, blockade of receptor by RNA interference (RNAi) may inhibit the entry and pathogenesis of CVB in cardiac cells. The present study was aimed to determine the effect of CAR small dsRNA (siRNA) on coxsackieviral load and CAR expression in coxsackievirus-infected cardiomyocytes. Methods: Transfection efficiency in rat cardiomyocytes (H9c2) was dete...
Source: Indian J Med Res - October 1, 2017 Category: Research Authors: Sharma M, Mishra B, Saikia UN, Bahl A, Ratho RK Tags: Indian J Med Res Source Type: research

Knockdown of human telomerase reverse transcriptase induces apoptosis in cervical cancer cell line.
Abstract Background & objectives: : Human telomerase reverse transcriptase (hTERT) is the catalytic subunit of telomerase enzyme that maintains telomere ends by the addition of telomeric repeats to the ends of chromosomal DNA, and that may generate immortal cancer cells. Hence, the activity of telomerase is raised in cancer cells including cervical cancer. The present study aimed to validate the unique siRNA loaded chitosan coated poly-lactic-co-glycolic acid (PLGA) nanoparticle targeting hTERT mRNA to knock down the expression of hTERT in HeLa cells. Methods: : The siRNA loaded chitosan coated polylactic...
Source: The Indian Journal of Medical Research - February 28, 2019 Category: Biomedical Science Authors: Nagapoosanam AL, Ganesan N, Umapathy D, Moorthy RK, Arockiam AJV Tags: Indian J Med Res Source Type: research

Computational study and design of effective siRNAs to silence structural proteins associated genes of Indian SARS-CoV-2 strains
Comput Biol Chem. 2022 Apr 29;98:107687. doi: 10.1016/j.compbiolchem.2022.107687. Online ahead of print.ABSTRACTSARS-CoV-2 is a highly transmissible and pathogenic coronavirus that first emerged in late 2019 and has since triggered a pandemic of acute respiratory disease named COVID-19 which poses a significant threat to all public health institutions in the absence of specific antiviral treatment. Since the outbreak began in March 2020, India has reported 4.77 lakh Coronavirus deaths, according to the World Health Organization (WHO). The innate RNA interference (RNAi) pathway, on the other hand, allows for the development...
Source: Computational Biology and Chemistry - May 10, 2022 Category: Bioinformatics Authors: Premnath Madanagopal Harshini Muthukumar Kothai Thiruvengadam Source Type: research

Ablation of DJ-1 enhances oxidative stress by disturbing the function of mitochondria in epidermal melanocytes
Conclusions: DJ-1 plays a role in maintaining the antioxidative capacity in epidermal melanocytes.
Source: Indian Journal of Dermatology - February 24, 2020 Category: Dermatology Authors: Man Li Fang Wang Juan Du Lijuan Wang Jianzhong Zhang Xiaolan Ding Source Type: research

First identification of PODXL nonsense mutations in autosomal dominant focal segmental glomerulosclerosis
In this study, we report the identification of a heterozygous nonsense PODXL mutations (Arg326X) in a Chinese pedigree featured by proteinuria and renal insufficiency with AD inheritance by whole exome sequencing (WES). Total mRNA and PODXL protein abundance were decreased in available peripheral blood cell samples of two affected patients undergoing hemodialysis, compared to those in healthy controls and hemodialysis controls without PODXL mutation. We identified another novel PODXL heterozygous nonsense mutation (c.C1133G; p.Ser378X) in a British-Indian pedigree of AD-FSGS by WES. In vitro study showed that, human embryo...
Source: Clinical Science - December 6, 2018 Category: Biomedical Science Authors: Lin, F.-J., Yao, L., Hu, X.-Q., Bian, F., Ji, G., Jiang, G.-R., Gale, D. P., Ren, H.-Q. Tags: PublishAheadOfPrint Source Type: research

Characterization of In vitro inhibitory effects of consensus short interference RNAs against non-structural 5B gene of hepatitis C virus 1a genotype
Conclusions: Stable Huh-7 cells persistently expressing NS5B protein should be helpful for molecular pathogenesis of HCV infection and development of anti-HCV drug screening assays. The siRNA was effective against NS5B and could be considered as an adjuvant therapy along with other promising anti-HCV regimens.
Source: Indian Journal of Medical Microbiology - March 17, 2019 Category: Microbiology Authors: Imran Shahid Waleed Hassan Almalki Munjed M Ibrahim Sultan Ahmad Alghamdi Mohammed H Mukhtar Shaia Saleh R. Almalki Saad Ahmed Alkahtani Mohammad S Alhaidari Source Type: research

Inhibition of γ/β Globin Gene Switching in CD 34 +  Derived Erythroid Cells by BCL11A RNA Silencing
In this study, the possibility of increasing HbF in the CD34+ derived erythroid cells was investigated by BCL11A inhibition using specific small-interfering RNAs (siRNAs). Human peripheral blood-derived hematopoietic stem cells were isolated and differentiated to erythroid cells. Erythroid maturation was investigated using cell morphology parameters and flow cytometry analysis of CD235a expression On day 20, siRNA complementary to BCL11A was transfected to differentiating cells via electroporation. BCL11A expression was evaluated through real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and e...
Source: Indian Journal of Hematology and Blood Transfusion - May 12, 2019 Category: Hematology Source Type: research

67PPARP inhibition enhances cisplatin sensitivity in cervical cancer by modulating β-catenin signaling
ConclusionsOur data provides experimental evidence on the contribution of PARP-1 inhibition in enhancing the cytotoxicity of CDDP in cervical cancer cells. We also present novel findings on the suppression of β-catenin and its downstream signaling components by PARP-1 inhibitor. #: 5μM of CDDP was used both alone and in combination with PJ34; ##: A gradient of CDDP from 0-15μM was used to evaluate the combined effect of PJ34 and CDDP on IC50 value of CDDP. p: *<0.05, **<0.01. (H): HeLa; (S): SiHa.Legal entity responsible for the studyThe authors.FundingAll India Institute of Medical Sciences.DisclosureAll authors ...
Source: Annals of Oncology - October 1, 2019 Category: Cancer & Oncology Source Type: research

Differential effects of performance-enhancing drugs 'Methamphetamine' and 'hCG' on ex-vivo cultured primary blood mononuclear cells of male athletes.
CONCLUSION: This study demonstrated the differential effects of Meth and hCG on immune cells of athlete's blood. Meth acted as an inflammation and T cell dysfunction inducing agent, while hCG acted as an anti-inflammatory immunosuppressive molecule. PMID: 32124391 [PubMed - as supplied by publisher]
Source: Pharmacological Reports - March 1, 2020 Category: Drugs & Pharmacology Authors: Pramanik A, Das S, Khanna GL Tags: Pharmacol Rep Source Type: research

Indian Hedgehog regulates senescence in bone marrow-derived mesenchymal stem cell through modulation of ROS/mTOR/4EBP1, p70S6K1/2 pathway.
This study explored the role IHH in the senescence of BMSC obtained from human samples and senescent mice. Isolated BMSC were transfected with IHH siRNA or incubated with exogenous IHH protein and the mechanisms of aging and differentiation investigated. Moreover, the interactions between IHH, and mammalian target of rapamycin (mTOR) and reactive oxygen species (ROS) were evaluated using the corresponding inhibitors and antioxidants. BMSC transfected with IHH siRNA showed characteristics of senescence-associated features including increased senescence-associated β-galactosidase activity (SA-β-gal), induction of cell cycl...
Source: Aging - March 31, 2020 Category: Biomedical Science Authors: Al-Azab M, Wang B, Elkhider A, Walana W, Li W, Yuan B, Ye Y, Tang Y, Almoiliqy M, Adlat S, Wei J, Zhang Y, Li X Tags: Aging (Albany NY) Source Type: research