Erratum: Abstracts - Indian journal of medical microbiology - supplement 1 - 2019 - Volume 37
Indian Journal of Medical Microbiology 2019 37(3):457-458 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Source Type: research

Fungal infection in a post-renal transplant patient with Diaporthe
K Vichitra, Lokeshwari Gopal, Balajee Govindarao, V Chandrasekaran, Anupma Jyoti KindoIndian Journal of Medical Microbiology 2019 37(3):454-456 Post-renal transplant fungal infections continue to be a major cause of mortality and morbidity. Universally reported fungi are Candida, especially Candida albicans, Cryptococcus, Aspergillus, Trichophyton rubrum and Pityriasis versicolor. Here, we report a case of infection caused by a rare fungus Diaporthe. It is an endophyte reported as plant pathogens and infrequently in humans and mammals. The patient was a renal transplant recipient on immunosuppressant. He had hypothyroidis...
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: K Vichitra Lokeshwari Gopal Balajee Govindarao V Chandrasekaran Anupma Jyoti Kindo Source Type: research

Paradoxical upgradation response in non-HIV tuberculosis: Report of two cases
Meghana Nathani Kabra, Thrinadh KunapareddyIndian Journal of Medical Microbiology 2019 37(3):450-453 A paradoxical upgradation response in tuberculosis (TB) is defined as the worsening of a pre-existing tubercular lesion or the appearance of a new lesion in a patient whose clinical symptoms initially improved with anti-TB treatment. A paradoxical response is common in HIV patients in the form of immune reconstitution inflammatory syndrome. A similar kind of response can also be seen in immunocompetent patients. Here, we present two cases of non-HIV TB who initially improved with antitubercular therapy (ATT) but worsened t...
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Meghana Nathani Kabra Thrinadh Kunapareddy Source Type: research

Utility of Xpert & #174; MTB/RIF in the diagnosis of extrapulmonary tuberculosis
Vidya Krishna, Ram Gopalakrishnan, Anil Tarigopula, Senthur Nambi Panchatcharam, Suresh Kumar Dorairajan, Ramasubramanian VenkatasubramanianIndian Journal of Medical Microbiology 2019 37(3):448-449 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Vidya Krishna Ram Gopalakrishnan Anil Tarigopula Senthur Nambi Panchatcharam Suresh Kumar Dorairajan Ramasubramanian Venkatasubramanian Source Type: research

Group B Streptococcus vaginal colonisation in pregnant women at a tertiary care hospital in Mumbai
Shalmali Deshpande, Tanu Singhal, Suchitra N Pandit, Sweta ShahIndian Journal of Medical Microbiology 2019 37(3):446-447 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Shalmali Deshpande Tanu Singhal Suchitra N Pandit Sweta Shah Source Type: research

Determination of Biofilm-Forming Capacity of Otopathogens Isolated from Discharging Ears in Children with Chronic Otitis Media
This study describes the microorganisms isolated from the middle ear and nasopharynx of children with chronically discharging ears. Middle ear and nasopharyngeal swabs from 89 children were studied, and the microorganisms isolated were assessed for biofilm-forming ability. Methicillin-susceptible Staphylococcus aureus was common in the nasopharynx, while the middle ear showed predominantly pseudomonas and Methicillin-resistant S. aureus. Pseudomonas aeruginosa showed strong biofilm formation, whereas Escherichia coli, Proteus sp. and Providentia sp. were weak biofilm producers. S. aureus isolates were negative for biofilm ...
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Zoremsangi Ralte P Naina Anushree Amladi Mary John Shalini Anndan Ajoy Mathew Varghese Source Type: research

Phylogenetic diversity of Orientia tsutsugamushi isolates in patients with scrub typhus in Bengaluru, India
Sneha K Chunchanur, Shwetha J Venugopal, R Ambica, B DakshayaniIndian Journal of Medical Microbiology 2019 37(3):438-441 Scrub typhus has re-emerged as an important cause of acute febrile illness in India. There is a dearth of information on strain diversity of Orientia tsutsugamushi from Karnataka, India, hence the present study sought to address this issue. One hundred clinically suspected cases of scrub typhus/rickettsiosis (as per the DHR-ICMR guidelines) were included. Nested-polymerase chain reaction (PCR) for 56-kDa gene and phylogenetic analysis was performed. PCR was positive in 22 cases and phylogenetic analysis...
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Sneha K Chunchanur Shwetha J Venugopal R Ambica B Dakshayani Source Type: research

Utility of QuantiFERON & #174;-TB gold In-Tube test compared with tuberculin skin test in diagnosing tuberculosis in Indian children with malnutrition
Deepthi Boddu, Valsan Philip Verghese, Joy Sarojini Michael, Anila Chacko, Visali JeyaseelanIndian Journal of Medical Microbiology 2019 37(3):433-437 This prospective cohort study was conducted to compare the accuracy of QuantiFERON®-TB (QFT) Gold In-Tube test and tuberculin skin test (TST) in diagnosing tuberculosis (TB) in predominantly bacille Calmette–Guerin-vaccinated children with a high incidence of malnutrition. The sensitivity of the QFT versus the TST was 69.6% versus 52.9% for WHO-defined TB, with specificity of 86% versus 78.3%, respectively. The concordance of the TST and QFT was 79...
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Deepthi Boddu Valsan Philip Verghese Joy Sarojini Michael Anila Chacko Visali Jeyaseelan Source Type: research

Melioidosis in Odisha: A clinico-microbiological and epidemiological description of culture-confirmed cases over a 2-year period
Bijayini Behera, Srujana Mohanty, Ashoka Mahapatra, Vinay Kumar Hallur, Baijayantimala Mishra, Anupam Dey, Rajesh Kumar, Tushar K Mishra, Prakash K Sasmal, Mithilesh Sinha, Prasanta R Mohapatra, Manoj K Panigrahi, C Preetam, Rashmi Ranjan DasIndian Journal of Medical Microbiology 2019 37(3):430-432 Melioidosis is an emerging infectious disease in India mostly reported from South-western coastal Karnataka and North-eastern Tamil Nadu. We speculate the existence of another major hidden focus in Odisha, one of the eastern coastal states. The clinico-epidemiological features of 47 culture-confirmed melioidosis at a tertiary c...
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Bijayini Behera Srujana Mohanty Ashoka Mahapatra Vinay Kumar Hallur Baijayantimala Mishra Anupam Dey Rajesh Kumar Tushar K Mishra Prakash K Sasmal Mithilesh Sinha Prasanta R Mohapatra Manoj K Panigrahi C Preetam Rashmi Ranjan Das Source Type: research

Seroprevalence of brucellosis in Western Rajasthan: A study from a tertiary care centre
Yashik Bansal, Alisha Aggarwal, Ravisekhar Gadepalli, Vijaya Lakshmi NagIndian Journal of Medical Microbiology 2019 37(3):426-432 Brucellosis is a significant bacterial zoonotic disease with a high seroprevalence in low-to-middle-income countries where there is a significant contact of humans with animals. This prospective study was conducted to observe the seroprevalence of brucellosis in 75 symptomatic patients with pyrexia of unknown origin (PUO) and 75 high-risk individuals (10 veterinarians, 15 milkmen and 50 healthy contacts of symptomatic patients) with possible exposure to brucellosis. Serum samples collected from...
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Yashik Bansal Alisha Aggarwal Ravisekhar Gadepalli Vijaya Lakshmi Nag Source Type: research

Insights to the diphtheria toxin encoding prophages amongst clinical isolates of Corynebacterium diphtheriae from India
Dhiviya Prabaa Muthuirulandi Sethuvel, Nithya Subramanian, Agila Kumari Pragasam, Francis Yesurajan Inbanathan, Prashant Gupta, Jaichand Johnson, Naresh Chand Sharma, Nanda Hemvani, Balaji Veeraraghavan, Shalini Anandan, Lucky SangalIndian Journal of Medical Microbiology 2019 37(3):423-425 Diphtheria is a dreadful disease caused by Corynebacterium diphtheriae. Lysogenised bacteriophages carrying toxin gene in C. diphtheriae can make the strain toxigenic. However, such phage disseminates the toxin genes to other strains when it undergoes lytic phase. As little is known about the phage diversity in C. diphtheriae in India, ...
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Dhiviya Prabaa Muthuirulandi Sethuvel Nithya Subramanian Agila Kumari Pragasam Francis Yesurajan Inbanathan Prashant Gupta Jaichand Johnson Naresh Chand Sharma Nanda Hemvani Balaji Veeraraghavan Shalini Anandan Lucky Sangal Source Type: research

Diverse aminoglycoside phosphotransferase types conferring aminoglycoside resistance in Enterobacteriaceae: A single-centre study from Northeast India
This study was able to identify diverse aph types in a single centre and their corresponding phenotypic trait. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Jayalaxmi Wangkheimayum Mohana Bhattacharjee Bhaskar Jyoti Das K Melson Singha Debadatta Dhar Chanda Debadatta Dhar Chanda Deepshikha Bhowmik Amitabha Bhattacharjee Source Type: research

Occurrence of viral gastroenteritis in children below 5 years: A hospital-based study from Assam, India
Shalini Goldar, Gitika Rajbongshi, Kailash Chamuah, Syed Tanwir Alam, Ajanta SharmaIndian Journal of Medical Microbiology 2019 37(3):415-417 Viral gastroenteritis is an important cause of mortality and morbidity in children under 5 years of age. Many a time, these cases go unnoticed causing immense scarcity of data on viral diarrhoea. The study aimed to determine the occurrence of viral gastroenteritis among children below 5 years and the aetiological viral agents. Stool samples were collected from patients suffering from acute gastroenteritis. Real-time polymerase chain reaction was done for detection of rotavirus, adeno...
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Shalini Goldar Gitika Rajbongshi Kailash Chamuah Syed Tanwir Alam Ajanta Sharma Source Type: research

VP1-binding protein glucose-regulated protein 78 as an important mediator for enterovirus 71 infecting human brain microvascular endothelial cells
Conclusion: These findings provided evidence that GRP78 plays an important role during the progression of EV71 infection as a mediator in HBMEC. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Wenying Luo Peng Liang Santhosh Puthiyakunnon Lawei Yang Cao Hong Source Type: research

Dynamics of the occurrence of influenza in relation to seasonal variation in Chennai, Tamil Nadu: A 7 -year cumulative study
Conclusion: In Chennai, Tamil Nadu, influenza positivity started with the onset of monsoon and peaks during the postmonsoon months throughout the study period. The assessment of meteorological factors compounding influenza activity can help in raising alerts to the public health officials of impending disaster which suggests that Influenza vaccination can be initiated before monsoon months in South India. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Ramesh Kiruba BV Suresh Babu AK Sheriff P Gunasekaran CP Anupama N Saran V Senthil Kumar P Padmapriya N Nivas Chakravarthy Krishnasamy Kaveri Source Type: research

Emergence of dengue virus 4 as the predominant serotype during the outbreak of 2017 in South India
Conclusions: Overall this study documents the emergence of DENV4 as the major serotype in circulation, replacing DENV1, 2 and 3 which had been previously reported from Tamil Nadu and Puducherry. This substantiates the need for continuous monitoring in endemic countries like India, where such data may impact the formulation of vaccine policy for dengue. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: P Ferdinamarie Sharmila K Vanathy Barathidasan Rajamani Venkatesh Kaliaperumal Rahul Dhodapkar Source Type: research

Hepatitis B virus X protein: The X factor in chronic hepatitis B virus disease progression
Conclusion: In HBx gene, aminoacid substitutions at positions 127, 130, 131 are associated with poor expression of HBeAg. We suggest screening for HBx aminoacid substitutions especially in patients with HBeAg negative chronic HBV infection to predict the clinical outcome and enable early treatment to prevent disease progression. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Monika Mani Shanthi Vijayaraghavan Gopalsamy Sarangan Ramya Barani Priya Abraham Padma Srikanth Source Type: research

Diagnostic performance of mini parasep & #174; solvent-free foecal parasite concentrator for the diagnosis of intestinal parasitic infections
Conclusion: Mini Parasep® SF technique is simple, rapid and less cumbersome than conventional diagnostics, making it suitable for routine use. In addition, it offers higher sensitivity and better background clearance than both direct stool examination and FEM. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Abhishek Mewara Sumeeta Khurana Swati Gupta Vimal S Munda Shreya Singh Rakesh Sehgal Source Type: research

Central line-associated bloodstream infections in cancer patients: An experience from a tertiary care cancer centre
Conclusion: Over the last 20 years, an epidemiologic shift has occurred among CLABSI in cancer patients. These findings should be considered with the development of interventions that will prevent Gram-negative CLABSI after CVC insertion. To our knowledge, this is the first study reporting data on the incidence of CLABSIs in cancer patients at tertiary care hospital from North Indian hospital. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Sonu Kumari Agrawal Hitender Gautam Abdul Hakim Choudhary Bimal Kumar Das Lalit Kumar Arti Kapil Source Type: research

Frequencies of regulatory subsets of CD4 & #43; TH cells in peripheral blood in Mycobacterium Tuberculosis-Infected individuals and healthy contacts in a high-burden setting from Assam, Northeast India
Conclusion: CD4+ TH cells change dynamically to regulatory subsets depending on the status of infection and a shift of response towards excessive regulatory T-cells, and PD-1/PD-L1 production may help in the development of active infection in latently infected individuals. These immunological parameters may be used, as potential biomarkers to see the changing dynamics of Mtb infection. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Lahari Saikia Trinayan Deka Pranjal Deori Rasmi Roy Md Ezaz Hussain Reema Nath Source Type: research

Cross-country transport and isolation and identification of Streptococcus pneumoniae by use of alternate sources of blood supplemented media among laboratories in India
Conclusions: The study clearly elucidates the ability of laboratories for isolation of S. pneumoniae on human blood agar in resource limited settings. The results highlight the difficulties inherent in correctly identifying pathogens in mixed cultures in needs improvement using standardized tests across the study centers. The study also reaffirms the ability to transport biological specimens over long geographical distances without loss. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Satish Kumar Amarnath Sangeeta Joshi Madhuwanti N Abhyankar Ranjeeta Adhikary HB Beena TD Chugh KD Gandhi Vivek Hittinahalli VA Indumathi Mukhopadhyay Rajavari S Muralidharan SS Rao I Roy N Saini Source Type: research

Clonal similarities and sequence-type diversity of invasive and carriage Streptococcus pneumoniae in India among children under 5 Years
Conclusion: The vaccine serotypes were the predominant ones found to be associated with IPD, PMEN clones, new STs and antimicrobial resistance. Accordingly, PCV13 is expected to provide invasive serotype coverage of 75% in Indian children less than 5 years. This study provides baseline serotype and sequence type data prior to the introduction of PCV in South India. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Rosemol Varghese Ayyanraj Neeravi Nithya Subramanian B Pavithra A Kavipriya Jones Lionel Kumar CP Girish Kumar Yuvraj Jeyraman G Karthik Valsan P Verghese Balaji Veeraraghavan Source Type: research

Seroprevalence of Toxoplasmosis at a Tertiary Care Centre in North India from 2004 to 2014
Conclusion: Toxoplasma infection is highly prevalent in the population of North India across various clinical categories of patients. Future studies focusing on continuous monitoring of seroprevalence trends and elucidation of the risk factors associated with seropositivity in more defined groups of patients are needed. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Abhishek Mewara Shreya Singh Sumeeta Khurana Parakriti Gupta Rakesh Sehgal Source Type: research

Detection and molecular typing of campylobacter isolates from human and animal faeces in coastal belt of Odisha, India
Conclusion: The present study showed the incidence of Campylobacter infections in human and different animal species in and around Bhubaneswar, Odisha. The analysis suggested that domestic animals can be the potential sources for human campylobacteriosis in the region. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Nirmal Kumar Mohakud Saumya Darshana Patra Subrat Kumar Priyadarshi Soumyaranjan Sahu Namrata Misra Arpit Kumar Shrivastava Source Type: research

Geographically distinct North-East Indian Helicobacter pylori strains are highly sensitive to clarithromycin but are levofloxacin resistant
Conclusions: Resistance for clarithromycin was less in North-East Indian strains but high for levofloxacin indicating that first-line therapy may be best and effective for eradication of H. pylori in this region. This study is the first report that showed antibiotic susceptibility pattern for clarithromycin and levofloxacin by mutation analysis. By partial sequencing of 23s rRNA and gyraseA gene, we found that North-East Indian strains are geographically distinct. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Shweta Mahant Amresh Kumar Sharma Valentina Gehlot Asish Kumar Mukhopadhyay Ayushi Chhawchharia Sangitanjan Dutta Anil Agarwal Anup Som Kunal Das Rajashree Das Source Type: research

Clonal clusters and virulence factors of methicillin-resistant Staphylococcus Aureus: Evidence for community-acquired methicillin-resistant Staphylococcus Aureus infiltration into hospital settings in Chennai, South India
Conclusion: A total of 769 isolates of S. aureus isolates were studied. The prevalence of MRSA was found to be 7.17%, 81.67%, 58.33% and 22.85% for groups a, b, c and d, respectively. Of the four SCCmec types (I, III, IV and V) detected, SCCmec V was found to be predominant. Panton-Valentine leucocidin toxin genes were detected among MRSA isolates harbouring SCCmec IV and V. A total of 78 spa types were detected, t657 being the most prevalent. 13 MLST types belonging to 9 CC were detected. CC1 (ST-772, ST-1) and CC8 (ST238, ST368 and ST1208) were found to be predominant among MRSA. CA-MRSA isolates with SCC...
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Nagarajan Abimannan G Sumathi OR Krishnarajasekhar Bhanu Sinha Padma Krishnan Source Type: research

Protocol for developing a surveillance system for surgical site infections
Conclusion: Surveillance is an essential tool to reduce this burden. It is also an important primary step in recognizing problems and priorities, and it plays a crucial role in identifying risk factors for SSI and to be able to target modifiable risk factors. Therefore, it is imperative to establish reliable systems for surveillance of HAIs, to regularly estimate the actual burden of HAIs, and to use these data for developing indigenous preventive measures, tailored to the country's priorities. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Purva Mathur Samarth Mittal Vivek Trikha Ayush Lohiya Surbhi Khurana Sonal Katyal Nidhi Bhardwaj Sushma Sagar Subodh Kumar Rajesh Malhotra Kamini Walia Source Type: research

Assessment of core capacities for antimicrobial stewardship practices in indian hospitals: Report from a multicentric initiative of global health security agenda
Conclusion: The hospitals included a mix of public and private sector hospitals. The network average of positive responses for leadership support was 45%, for accountability; the score was 53% and for key support for AMSP, 58%. Policies to support optimal antibiotic use were present in 59% of respondents, policies for procurement were present in 79% and broad interventions to improve antibiotic use were scored as 33%. A score of 52% was generated for prescription-specific interventions to improve antibiotic use. Written policies for antibiotic use for hospitalised patients and outpatients were p...
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Mathur Purva Guleria Randeep Malhotra Rajesh C Misra Mahesh Gupta Sunil Kumar Subodh Sagar Sushma Wig Naveet Garg Pramod Kapil Arti Khurana Surbhi Katoch Omika Katyal Sonal Sahu Manoj Chakrabarti Arunaloke Ray Pallab Biswal Manisha Taneja Neelam Rupali Pr Source Type: research

Topical antimicrobial therapy: Current status and challenges
Pallab Ray, Shreya Singh, Swati GuptaIndian Journal of Medical Microbiology 2019 37(3):299-308 Topically applied antibacterial agents are widely used. Opinions regarding the clinical efficacy of topical antibiotics are conflicting, and for most indications, alternative oral therapies are available. Topical application has many potential advantages over systemic therapy that includes high and sustained concentrations of drug directly at the infected site, low quantity of antibiotic needed, better compliance, fewer systemic side effects and potentially less chance of antimicrobial resistance. Despite these advantages, an im...
Source: Indian Journal of Medical Microbiology - January 29, 2020 Category: Microbiology Authors: Pallab Ray Shreya Singh Swati Gupta Source Type: research

Combined treatment modality for phthiriasis palpebrarum
Chitaranjan Mishra, Usha Kim, Mulasthanam Sai DheeraIndian Journal of Medical Microbiology 2019 37(2):296-297 Phthiriasis palpebrarum (PP) is the infestation of eyelids caused by the ectoparasite Phthirus pubis, frequently misdiagnosed as allergic conjunctivitis, blepharitis or dermatitis. There is no standard treatment of choice although various treatment modalities have been described. A 6-year-old male child with PP was successfully treated with local application of 20% fluorescein solution over the eyelashes and eyebrows of both the eyes, followed by the mechanical removal of all parasites and trimming of the eyel...
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Chitaranjan Mishra Usha Kim Mulasthanam Sai Dheera Source Type: research

Multifocal hepatic abscesses in immunocompetent patient due to Bartonella henselae: Case report with review of literature
Sonu Kumari Agrawal, Prasenjit Das, Shalimar , Gupta Swatantra, Rama ChaudhryIndian Journal of Medical Microbiology 2019 37(2):292-295 To the best of our knowledge, this is the first case of multifocal hepatic abscesses in a young immunocompetent adult from India, which was successfully treated with hepatectomy and short course of oral antibiotic regimen. Publishing further such case reports will provide more clarity regarding the clinical significance of the disease, including associated risk factors and appropriate treatment. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Sonu Kumari Agrawal Prasenjit Das Shalimar Gupta Swatantra Rama Chaudhry Source Type: research

Ocular infection by a psychrophile: Pseudomonas fluorescens
We report a case, wherein we serendipitously isolated Pseudomonas fluorescens – an organism that prefers lower temperatures for optimal growth (psychrophilic) in the environment – from eviscerated contents of an eye with total corneal melt. This case highlights the need for being vigilant for organisms with different temperature sensitivities in culture media than that found in routine protocols. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Sanchita Mitra Suryasnata Rath Sujata Das Soumyava Basu Source Type: research

A typical Escherichia coli: A Dilemma of a Clinical Diagnostic Laboratory
Tonushyam Sonowal, Malini ShariffIndian Journal of Medical Microbiology 2019 37(2):287-288 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Tonushyam Sonowal Malini Shariff Source Type: research

Increase in Chlamydia trachomatis genital and extra-genital infections in Indian males
Sonu Kumari Agrawal, Jyoti Rawre, Neena Khanna, Benu DhawanIndian Journal of Medical Microbiology 2019 37(2):285-286 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Sonu Kumari Agrawal Jyoti Rawre Neena Khanna Benu Dhawan Source Type: research

Detection of cytomegalovirus disease by real-time quantitative PCR targeting immediate early gene (ppUL83) in different samples among post-renal-transplant recipients
Ramya Barani, Vigna Seshan, Yazhini Ravi, Periasamy Soundararajan, Gunasekaran Palani, Padma SrikanthIndian Journal of Medical Microbiology 2019 37(2):281-284 Renal transplantation is a treatment option for end-stage renal disease (ESRD). Cytomegalovirus (CMV) infection was analysed among symptomatic and asymptomatic post-renal-transplant recipients (PRTRs). A total of 30 PRTRs were enrolled. DNA was extracted and quantitative real-time PCR for CMV (CMV R-Gene, France) targeting ppUL83 gene was performed on whole blood, urine and saliva. The detection rate of CMV was found to be 27% (n = 8) in different samples, i...
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Ramya Barani Vigna Seshan Yazhini Ravi Periasamy Soundararajan Gunasekaran Palani Padma Srikanth Source Type: research

Clinico-epidemiological analysis of scrub typhus in hospitalised patients presenting with acute undifferentiated febrile illness: A hospital-based study from Eastern India
Bijayini Behera, Manisha Biswal, Rashmi Ranjan Das, Anupam Dey, Jayanti Jena, Sagarika Dhal, Srujana Mohanty, Baijayantimala Mishra, Ashok Kumar PraharajIndian Journal of Medical Microbiology 2019 37(2):278-280 Acute undifferentiated febrile illness (AUFI) constitutes the predominant cause of healthcare seeking in Odisha. This prospective study was conducted to analyse the clinical, epidemiological and laboratory profile of scrub typhus patients presenting with AUFI from January to December 2017. Four hundred and thirty-two samples were tested for dengue, malaria, scrub typhus and enteric fever. Scrub typhus was overall t...
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Bijayini Behera Manisha Biswal Rashmi Ranjan Das Anupam Dey Jayanti Jena Sagarika Dhal Srujana Mohanty Baijayantimala Mishra Ashok Kumar Praharaj Source Type: research

Gut microbiota and its mysteries
Premalatha Pushpanathan, Gifty Sara Mathew, Sribal Selvarajan, Krishna G Seshadri, Padma SrikanthIndian Journal of Medical Microbiology 2019 37(2):268-277 Gut microbiota are microorganisms that inhabit the gut; they coexist peacefully with the host, thereby contributing to the health and well-being of individuals. Bacteroidetes and Firmicutes largely dominate the gut microbial flora. The intestinal flora promotes intestinal mucosal integrity, provides essential nutrients such as vitamins and enzymes, protects the body against pathogens and produces antimicrobial peptides such as defensins, C-type lectins, cathelicidins, t...
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Premalatha Pushpanathan Gifty Sara Mathew Sribal Selvarajan Krishna G Seshadri Padma Srikanth Source Type: research

Ultrasound gel as a source of hospital outbreaks: Indian experience and literature review
Conclusion: This report highlights the importance of early identification of an outbreak, prompt response of the ICU and infection control teams, sound environmental and epidemiological surveillance methods to identify the source and stringent infection control measures to control the outbreak. Contaminated ultrasound gel can be a potential source for healthcare-associated infection, which cannot be overlooked. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Dhanalakshmi Solaimalai Naveen Kumar Devanga Ragupathi Kala Ranjini Hema Paul Valsan P Verghese Joy Sarojini Michael Balaji Veeraraghavan Ebor Jacob James Source Type: research

Predominance of high-risk human papillomavirus genotype 16 and 39 in women with premalignant and malignant cervical pathology from Raipur, Chhattisgarh: Clinical evaluation of tagging oligonucleotide cleavage and extension mediated genotyping assay
Conclusion: Anyplex HR-HPV assay is arguably the useful assay for better patient management and can be useful for HR-HPV screening by its unique individual genotype identification of all HR-HPV. Finding of HPV 16, 39, 18, 33 and coinfection of 16,18 and 16, 18, 33 in CIN and CC would help vaccine manufacturer to design specific future HPV polyvalent vaccine preparation to curb down the CC-associated mortality. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Sanjay Singh Negi Anudita Bhargava Priyanka Singh Sarita Aggarwal Nighat Hussain Padma Das Source Type: research

Genetic diversity of human respiratory syncytial virus in children with acute respiratory infections in Chennai, South India
Conclusion: This study underlines the genetic diversity of HRSV and emphasises the need for continued molecular surveillance for infection management and prevention strategies. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Anusha Hindupur Thangam Menon Prabu Dhandapani Source Type: research

Evaluation of loop-mediated isothermal amplification assay for detection and typing of human papilloma virus 16 and 18 from endocervical samples
Conclusions: We evaluated the LAMP assay for simultaneous detection and typing of HPV 16 and 18. The assay had good agreement with NM-PCR for detection of both HPV 16 and 18. The LAMP assay is a promising tool for HPV genotyping along with routine cervical cytology, especially in resource-constrained settings. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Nagaraja Mudhigeti Usha Kalawat Narendra Hulikal Meenakshi Kante Source Type: research

A descriptive study on prevalence pattern of Japanese encephalitis in State of Manipur
Conclusions: The changing pattern of JE cases among different age groups was also observed in our study. The present study reveals the changing pattern of the prevalence of JE in the State of Manipur and initiated a systematic approach of JE surveillance also highlights the need for further expanding of surveillance across the state. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Leimapokpam Shivadutta Singh Huidrom Lokhendro Singh Natasha Thokchom RK Manojkumar Singh Source Type: research

Infectious hepatitis: A 3-year retrospective study at a tertiary care hospital in India
Conclusions: A higher seroprevalence of HEV as compared to HAV together with a co-infection rate of 2.07% mandates screening for HEV in all suspected cases of acute hepatitis, particularly pregnant women in whom the outcomes of HEV infection are poor. Health and civic authorities should make necessary efforts to counter epidemic or outbreak situations, thus reducing morbidity, mortality and economic burden. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Arghadip Samaddar Shripad Taklikar Pradnya Kale Chaya A Kumar Sujata Baveja Source Type: research

The prevalence of anti-hepatitis C antibody among acute febrile illness cases in Idar Taluk, Gujarat, West India
Conclusion: The estimated seroprevalence of hepatitis C in Idar Taluk, Sabarkantha district, west India was 2.3%. HCV genotypes 1b and 3a were observed to be circulating in the study area. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Shekara Nikitha Sasidharanpillai Sabeena Sudandiradas Robin Dodia Hiren Varamballi Prasad Sushama Aswathyraj Santhosha Devadiga Jayaram Anup Govindakarnavar Arunkumar Source Type: research

Characteristics of treatment-na & #239;ve HBV-infected individuals with HIV-1 coinfection: A cross-sectional study from South India
Conclusion: These findings conclude that HIV-1 coinfection can have serious implications on the outcome of HBV-related liver disease. To the contrary, HBV infection had no consequence on the progression of HIV-1 disease but distinctly lowered CD4+ T-cells. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: John Paul Demosthenes Jaiprasath Sachithanandham Gnanadurai John Fletcher Uday George Zachariah George Mathew Varghese Hubert Darius John Daniel Lakshmanan Jeyaseelan Priya Abraham Rajesh Kannangai Source Type: research

Characterisation of virulence genes associated with pathogenicity in Klebsiella pneumoniae
Conclusion: Pathogenic K. pneumoniae can harbour single to multiple virulence genes. Invasive infection with even a single virulence gene-harbouring K. pneumoniae can lead to poor outcomes. Both multidrug-resistant (MDR) and non-MDR K. pneumoniae can harbour a variety of virulence genes. None of the virulence genes have a significant association with mortality. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: PA Remya M Shanthi Uma Sekar Source Type: research

Review of a 7-year record of the bacteriological profile of airway secretions of children with cystic fibrosis in North India
Conclusions: The pattern of airway colonisation in the Indian setting is different from the Caucasian population, and P. aeruginosa and Burkholderia cepacia complex appear early. Colonisation with P. aeruginosa benefits from therapy. In case of infection, care must be taken while initiating empiric therapy. It should be based on local antibiograms to prevent the emergence of resistant microbes. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Vikas Gautam Parinitha Kaza Joseph L Mathew Varpreet Kaur Megha Sharma Pallab Ray Source Type: research

An emerging threat of ceftriaxone-resistant non-typhoidal salmonella in South India: Incidence and molecular profile
Conclusion: This study shows that ceftriaxone resistance is mainly mediated by β-lactamases such as ESBL and AmpC. As the incidence of ceftriaxone resistance is rising gradually over the years, it is imperative to monitor the AMR in this species. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Agila Kumari Pragasam Shalini Anandan James John Ayyanraj Neeravi Vignesh Narasimman Dhiviya Prabaa Muthuirulandi Sethuvel Divyaa Elangovan Balaji Veeraraghavan Source Type: research

Assessment of efficacy of palm polymerase chain reaction with microscopy, rapid diagnostic test and conventional polymerase chain reaction for diagnosis of malaria
Conclusion: Palm PCR is sensitive, rapid and works on battery with simple laboratory facility requirements. Portable electrophoresis and transilluminator combined with Palm PCR could be implemented as an important diagnostic tool in resource-limited and rural areas. Similar studies with wider parameters in rural areas will help us evaluate and maybe establish Palm PCR as PCR platform of choice for such specific set-ups. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Paras Mahale Rajas Warke Mira Ramaiya Deepa Balasubramanian Suvin Shetty Ranjit Mankeshwar Abhay Chowdhary Source Type: research

Clinico-microbiological analysis of toxigenic clostridium difficile from hospitalised patients in a tertiary care hospital, Mangalore, Karnataka, India
Conclusion: The presence of toxigenic C. difficile in our locality is a matter of concern. Constant supervision, appropriate treatment and preventive measures are crucial in controlling C. difficile infection. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 19, 2019 Category: Microbiology Authors: Sherin Justin Beena Antony Source Type: research