The continuing scourge of congenital syphilis in the face of declining seropositivity: A challenge to control efforts
Srujana Mohanty, Tapas Som, Ashok Kumar PraharajIndian Journal of Medical Microbiology 2018 36(2):295-297 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Srujana Mohanty Tapas Som Ashok Kumar Praharaj Source Type: research

High prevalence of varicella seronegativity in nurses at a tertiary care private hospital in Mumbai, India
Sweta Shah, Tanu Singhal, Reshma Naik, Pooja ThakkarIndian Journal of Medical Microbiology 2018 36(2):294-295 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Sweta Shah Tanu Singhal Reshma Naik Pooja Thakkar Source Type: research

Oral administration of Klebsiella pneumoniae-specific bacteriophage eradicates the bacteria in albino mice
Ashutosh Chaturvedi, Gopal NathIndian Journal of Medical Microbiology 2018 36(2):293-294 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Ashutosh Chaturvedi Gopal Nath Source Type: research

Clinical spectrum, susceptibility profile, treatment and outcome of culture-confirmed brucellosis from South India
This study was conducted to determine the clinical manifestations, antibiotic susceptibility pattern, treatment and outcome of culture confirmed brucellosis. Adult patients with culture confirmed brucellosis who presented to a large teaching hospital in South India between 2009 and 2015 were included. A diagnosis of brucellosis was confirmed on automated culture. Clinical profile, laboratory parameters, drug susceptibility, treatment and outcome were documented by reviewing the medical records. The cohort comprised of 22 patients with mean ± SD age of 42 ± 13 years. Twenty one (95.5%) was male. Thirteen (59&#...
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Selvin Sundar Raj Mani Karthik Gunasekaran Ramya Iyyadurai John Antony Jude Prakash Balaji Veeraraghavan Ajay Kumar Mishra Kirti Sabnis Punitha John Victor Sherry Martin Vignesh Kumar Chandiraseharan Samuel George Hansdak George M Varghese Source Type: research

Alarming resistance of Neisseria gonorrhoeae in a tertiary care hospital of North India
Conclusion: Alarming increase in the resistance to commonly used antimicrobials for gonorrhoea in our study, especially of fluoroquinolones, is a clarion call for the urgent need for prudence in prescribing them. Observing the rampant resistance exhibited by N. gonorrhoeae, it is clear that the day is not far when it will acquire a superbug status and become intractable to treatment by the available antibiotics. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Mohit Sharma Meher Rizvi Richa Gupta Mohd Azam Haris M Khan Anjum Parvez Indu Shukla Source Type: research

Twenty-one episodes of peritonitis in a continuous ambulatory peritoneal dialysis patient: What is the root cause ?
Victorine B Nzana, Anusha Rohit, Deepu George, Madhusudan Vijayan, Milly Mathew, Sundar Sankaran, Palaniappan Nagarajan, Georgi AbrahamIndian Journal of Medical Microbiology 2018 36(2):282-284A 51-year-old female, with non-alcoholic liver cirrhosis, portal hypertension, type 2 diabetes mellitus, autosomal dominant polycystic kidney disease with a clipped cerebral aneurysm and chronic kidney disease stage 5 was on continuous ambulatory peritoneal dialysis (CAPD) for 6.5 years elsewhere. She came for opinion on continuation of CAPD as she had 21 episodes of peritonitis in 76 months. Her blood pressure was 80/50 mmHg. She was...
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Victorine B Nzana Anusha Rohit Deepu George Madhusudan Vijayan Milly Mathew Sundar Sankaran Palaniappan Nagarajan Georgi Abraham Source Type: research

Intraventricular haemorrhage as the first manifestation of congenital Cytomegalovirus infection
Malgorzata Sobolewska-Pilarczyk, Katarzyna Pawlak-Osinska, Sylwia Drewa, Beata Smok, Małgorzata PawlowskaIndian Journal of Medical Microbiology 2018 36(2):279-281Congenital Cytomegalovirus infection (CCMV) is the most common intrauterine infection. Early diagnosis of CCMV is hindered by three factors: There is no screening programme for CMV infection in pregnant women; a high percentage of infections in neonates are asymptomatic; the clinical signs of CCMV infection are uncharacteristic. The aim of this article is to analyse the clinical picture and course of CCMV treatment in a 3-week-old newborn, analyse adverse eve...
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Malgorzata Sobolewska-Pilarczyk Katarzyna Pawlak-Osinska Sylwia Drewa Beata Smok Ma & #322;gorzata Pawlowska Source Type: research

Identification of prevalent dengue serotypes by reverse transcriptase polymerase chain reaction and correlation with severity of dengue as per the recent World Health Organization classification (2009)
Conclusions: DENV-3 and DENV-2 serotypes are prevalent in the region and are associated with a more serious clinical profile than other serotypes. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Tanmay K Mehta Parul D Shah Source Type: research

Antibiotic resistance in Helicobacter pylori: A mutational analysis from a tertiary care hospital in Kashmir, India
Conclusions: Tetracycline and quinolones could be the antibiotics of choice in the eradication of H. pylori in this region, while recurrence of the infection with H. pylori could be expected among patients receiving either metronidazole or clarithromycin, for eradication therapy. DST should be done on a routine basis utilising both phenotypic and genotypic methods to prevent further emergence of resistance in this region. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Fayaz Ahmad Wani Gulnaz Bashir Mushtaq Ahmad Khan Showkat Ali Zargar Zubaida Rasool Qurteeba Qadri Source Type: research

Molecular investigation of staphylococcal cassette chromosome mec types and genotypic relations of methicillin-resistant staphylococci isolated from before and after hospital exposed students
Conclusion: Most of the isolates obtained after hospital training showed clonal similarity and seven had multiple SCCmec elements require further investigation for the possible mechanism. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Bora Ekinci Tuba Gokdogan Edgunlu Gulcin Bayramoglu Gulsen Ulucam Atay Source Type: research

Assessment of microbiological status after successful completion of intermittent revised national tuberculosis control programme directly observed treatment, short course regimen for microbiologically confirmed pulmonary tuberculosis cases: While new daily regimen going to be implemented in India
Conclusion: Till date, RNTCP does not follow up the patients for any period of time after successful completion of treatment. Through the present study, we could find relapse cases in 10% of the previously treated non-symptomatic patients. These unnoticed relapse cases have potential to spread TB and increase disease burden of country. Thus, we can conclude that RNTCP has to follow up the patients after successful treatment to determine whether they relapse. It is needed for the success of programme and control of the disease in the country. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Roumi Ghosh Sudipta Roy Md. Khalid Rashid Source Type: research

Intestinal cryptosporidiosis in renal transplant recipients: Prevalence, species detection and comparative evaluation of SSU rRNA and Cryptosporidium oocyst wall protein genes
Conclusions: Cryptosporidiosis is common amongst RT recipients, particularly those with diarrhoea. C. hominis is the most common species in the studied population. SSU rRNA PCR was more sensitive molecular method for the differentiation of Cryptosporidium species. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Ujjala Ghoshal Prabhat Ranjan Asmita Dey Uday Chand Ghoshal Source Type: research

Performance of an in-house real-time PCR assay for detecting Cytomegalovirus infection among transplant patients from a tertiary care centre
Conclusion: The in-house viral load assay had a better correlation with clinical findings compared to the commercial assay. Quality assessment of these assays was done by the United Kingdom National External Quality Assessment Scheme (UKNEQAS), an external proficiency testing programme, and by the National Institute for Biological Standard and Control (NIBSC) standard. For UKNEQAS and NIBSC standards, the bias between the assays was 0.73 log10and 0.85 log10, respectively. This difference is well within the acceptable range already reported in the literature. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Santhosh Kumar Duraisamy Shoba Mammen Sasi Kumar Reddy Lakshminarayan Susan Verghese Mahesh Moorthy Biju George Rajesh Kannangai Santosh Varghese Alok Srivastava Asha Mary Abraham Source Type: research

Co-circulation of four dengue serotypes at South Eastern Andhra Pradesh, India: A prospective study
Conclusion: This study reports the all four dengue serotypes' co-circulation. This is the first report from South Eastern Andhra Pradesh. Amongst four, DENV-2 was predominant followed by DENV-4. The information of predominant serotypes can guide in forecasting dengue outbreaks and improving control measures of vectors thus may be helpful in the prevention of outbreaks. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Rishi Gowtham Racherla Madhavi Latha Pamireddy Alladi Mohan Nagaraja Mudhigeti Padmalatha Anjaneyulu Mahalakshmi Umapathi Nallapireddy Usha Kalawat Source Type: research

Detection of anti-hepatitis C virus and hepatitis C virus RNA in dried blood spot specimens using Whatman No. 1 filter paper
Conclusions: DBS using Whatman No. 1 filter paper is quite reliable as serum for detection of anti-HCV. It can be useful in effective surveillance. However, it is not suitable for confirmation of chronic HCV infection. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Ritapa Ghosh Naba Kumar Hazarika Source Type: research

The effectiveness of sofosbuvir and daclatasvir in the treatment of hepatitis C in thalassaemia major patients and their effect on haematological factors
Conclusion: Combination of SOF and DAC is an effective and tolerable treatment regimen without affect on the amount of anaemia in patients and did not differentiate the need for blood transfusions. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Farhad Zamani Hossein Ajdarkosh Fahimeh Safarnezhad-Tameshkel Azita Azarkeivan Hossein Keyvani Farshad Naserifar Jamshid Vafaeimanesh Source Type: research

Lamivudine plus tenofovir versus lamivudine plus adefovir for the treatment of hepatitis B virus in HIV-coinfected patients, starting antiretroviral therapy
Conclusions: Adefovir plus lamivudine is an effective alternative of tenofovir plus lamivudine in long-term HBV treatment outcome in HIV/HBV coinfected patients. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Jayeeta Sarkar Debraj Saha Bhaswati Bandyopadhyay Bibhuti Saha Runu Chakravarty Subhasish Kamal Guha Source Type: research

Rapid detection of ethambutol-resistant Mycobacterium tuberculosis in clinical specimens by real-time polymerase chain reaction hybridisation probe method
Conclusions: Our real-time PCR hybridisation probe method promises for rapid detection of EMB-resistant MTB directly from clinical specimens. However, future studies and modifications of method by incorporating other potential loci along with targeted mutation (emb306) are still required to increase the sensitivity of method. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: DS Chauhan R Sharma D Parashar R Das P Sharma AV Singh PK Singh K Katoch VM Katoch Source Type: research

Polymorphisms in cytotoxic T-lymphocyte associated antigen 4 gene does not affect scytotoxic T-lymphocyte associated antigen 4 levels in human papillomavirus-infected women with or without cervical cancer
Conclusion: The present study suggests sCTLA-4 levels are not affected by a polymorphism at + 49 A>G CTLA-4. Hence, levels of CTLA-4 are similar in both CaCx cases and control group. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Priyanka Wagh Priyanka Kulkarni Shilpa Kerkar Himangi Warke Hemangi Chaudhari Kedar Deodhar Bharat Rekhi Hemant Tongaonkar Jayanti Mania-Pramanik Source Type: research

Prothrombotic state in HIV: A study on protein C, protein S, homocysteine and correlation with CD4 counts
Swati Khare, Rashmi Kushwaha, Ashutosh Kumar, Vimala Venkatesh, Himanshu D Reddy, Mili Jain, Mohd Yusuf, Uma Shankar SinghIndian Journal of Medical Microbiology 2018 36(2):201-206Introduction: Human immunodeficiency virus (HIV) may result in variable haematological manifestations. Thrombotic events are more common among HIV-infected persons than the general population, possibly due to the increased inflammatory/hypercoagulable state and presence of concurrent comorbidities. Aims and Objectives: (1) Screen for coagulation abnormalities in HIV-infected patients. (2) Detect certain prothrombotic factors such as deficiency of ...
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Swati Khare Rashmi Kushwaha Ashutosh Kumar Vimala Venkatesh Himanshu D Reddy Mili Jain Mohd Yusuf Uma Shankar Singh Source Type: research

Identification of Raoultella spp.: Comparison of three methods
Conclusion: It was established that MALDI-TOF MS method is reliable in identifying genus Raoultella. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Alicja Sekowska Agnieszka Mikucka Eugenia Gospodarek-Komkowska Source Type: research

Distribution of hepatitis C virus genotypes in Istanbul, Turkey
Conclusion: The majority of patients with HCV infection had genotype 1 (82.5%), followed by genotype 3, 2 and 4. Monitoring the change in HCV genotype distribution is critical for the development of effective strategies for HCV elimination. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Nuran Karabulut Sema Alacam Ayfer Yolcu Mustafa Onel Ali Agacfidan Source Type: research

Trends in antimicrobial resistance patterns of Group A streptococci, molecular basis and implications
Conclusion: The erythromycin and tetracycline resistance in GAS continues to exist at high levels and may be attributed to the over-prescription and use of these antibiotics. Our findings indicate that the use of these antibiotics especially macrolides as empiric therapy in penicillin-allergic patients may not be appropriate. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Tintu Abraham Sujatha Sistla Source Type: research

Molecular characterisation of virulent gene vacA in Helicobacter pylori clinical isolates from patients with gastroduodenal diseases in Assam, India
Conclusion: Prevalence of VacA s1m1 strains may account for high risk of transmission of this gastric pathogen and the overall risk of acquiring infection. Phylogenetic analysis results suggests the prevalence of high genetic diversity in our region. Our findings may aid in developing a better understanding of the genetic structure of H. pylori and the pathophysiology of associated diseases, thus facilitating the implementation of various treatment options. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Anisha Sarma Lahari Saikia Mituban Gogoi Kaushal Yadav Source Type: research

BK virus characterisation among HIV-1-Infected individuals and its association with immunosuppression
Conclusion: There was a higher frequency of BK viruria in HIV-1-infected individuals than among healthy controls and the positivity correlated with the degree of immunosuppression. There was no association of high VL with NCCR rearrangements in urine. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Subha Jagannath Jaiprasath Sachithanandham Veena V Ramalingam John Paul Demosthenes Asha M Abraham Anand Zachariah George M Varghese Rajesh Kannangai Source Type: research

Profile of respiratory pathogens causing acute respiratory infections in hospitalised children at Rajasthan a 4 year & #39;s study
Conclusion: RSV and S. pneumoniae were the most common pathogens detected, higher prevalence was observed in children <1 year of age. Viruses were predominant during winter months. The study helped to prepare diagnostic algorithm which will help in reducing diagnostic costs. However, further studies are required to assess whether viruses are bystander or real pathogens and include larger panel of bacteria and viruses for diagnosis. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: M Anjaneya Swamy Bharti Malhotra PV Janardhan Reddy Jitendra Tiwari Source Type: research

Prevention of healthcare-associated infections in low- and middle-income Countries: The & #39;bundle approach & #39;
Conclusion: With the escalating armamentarium of antimicrobial resistance, healthcare sector has to go back to the very basics of hospital infection control; develop, assess and implement bundles of prevention. These are cost-effective and easily adaptable, to cater to the increasing HCAIs and MDR infections in the LMICs. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Purva Mathur Source Type: research

WHO essential diagnostics list (2018): A revolutionary step in strengthening health laboratories at all levels of healthcare
Rajesh BhatiaIndian Journal of Medical Microbiology 2018 36(2):153-154 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Rajesh Bhatia Source Type: research

Carrier study and treatment of close contacts: A must for diphtheria eradication
Usha UdgaonkarIndian Journal of Medical Microbiology 2018 36(1):148-148 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Usha Udgaonkar Source Type: research

Client partner notification and management: A rational approach to reduce new HIV infections
Sunite A Ganju, Anil K KangaIndian Journal of Medical Microbiology 2018 36(1):147-148 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Sunite A Ganju Anil K Kanga Source Type: research

Septicemic listeriosis: An emerging food-borne illness in India ?
We report a case of sporadic food-borne listeriosis, in an otherwise healthy individual presenting with meningo-encephalitis. Prompt identification and appropriate antibiotic therapy led to a favorable outcome. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Angel T Miraclin Susmitha K Perumalla John Davis Prasad Thambu David Sudarsanam Source Type: research

Acute hepatitis due to Epstein & #8211;Barr virus with cross-reacting antibodies to cytomegalovirus
In this report, we present a 19-year-old patient presented with nausea, fatigue and jaundice. Her physical examination and laboratory tests revealed the diagnosis as acute hepatitis due to EBV with cross-reacting antibodies to cytomegalovirus. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Asli Karadeniz Zuhal Yesilbag Fatih & #214;ner Kaya Feride Sinem Akg & #252;n Source Type: research

Fungal keratitis caused by Laetisaria arvalis
Lakshey Dudeja, Lakshmipriya Jeganathan, N Venkatesh Prajna, Lalitha PrajnaIndian Journal of Medical Microbiology 2018 36(1):140-142A 53-year-old female presented with keratitis (right eye) after fall of insect 10 days back. The ulcer worsened in spite of aggressive treatment with topical natamycin (5%) and amphotericin-B (0.15%) eye drops and finally perforated. Iris tissue sealed the perforation site, and ulcer healed with formation of adherent leucoma. This case represents first reported case of keratitis caused by Laetisaria arvalis and stresses on aggressive course of keratitis caused by this fungus. Importanc...
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Lakshey Dudeja Lakshmipriya Jeganathan N Venkatesh Prajna Lalitha Prajna Source Type: research

A first-line antiretroviral therapy-resistant HIV patient with rhinoentomophthoromycosis
Rachita Dhurat, Rajendra J Kothavade, Anand KumarIndian Journal of Medical Microbiology 2018 36(1):136-139The Conidiobolus coronatus-related rhinoentomophthoromycosis in immunocompetent and immunocompromised (HIV negative) individuals has been treated successfully with antifungal drugs. However, C. coronatus infections in first-line antiretroviral therapy (ART)-resistant (HIV infected) individuals particularly with rhinoentomophthoromycosis have not been reported previously. Here, we describe a case of itraconazole non-responding rhinoentomophthoromycosis in an HIV-infected patient with first-line antiretroviral (ART) drug...
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Rachita Dhurat Rajendra J Kothavade Anand Kumar Source Type: research

Observation of a new pattern of mutations in gyrA and parC within Escherichia coli exhibiting fluroquinolone resistance
Nivedita Dasgupta, Deepjyoti Paul, Debadatta Dhar Chanda, Shiela Chetri, Atanu Chakravarty, Amitabha BhattacharjeeIndian Journal of Medical Microbiology 2018 36(1):131-135Therapeutic options with quinolones are severely compromised in infections caused by members of Enterobacteriaceae family. Mutations in chromosomal region are one of the major reasons for bacterial resistance towards this group of antibiotic. The aim of the study is to detect the mutations in gyr A and par C responsible for quinolone resistance among clinical isolates of Escherichia coli. A total of 96 quinolone-resistant clinical isolates of E. coli were...
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Nivedita Dasgupta Deepjyoti Paul Debadatta Dhar Chanda Shiela Chetri Atanu Chakravarty Amitabha Bhattacharjee Source Type: research

In silico and In vitro activity of ceftolozane/tazobactam against pseudomonas aeruginosa collected across Indian hospitals
In this study, we determined the antimicrobial susceptibility for a total of 149 clinical isolates of P. aeruginosa for the most commonly used antimicrobials including the new agent ceftolozane/tazobactam (C/T). Broth microdilution was performed to determine the minimum inhibitory concentration against various antimicrobials including C/T. Among the β-lactam/β-lactamase inhibitor, overall susceptibility was 67%, 55% and 51% for C/T, Piperacillin/Tazobactam (P/T) and Cefoperazone/Sulbactam, respectively. The variations in the susceptibility rates were noted among the three different β-lactam/β...
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Agila Kumari Pragsam D Thirumal Kumar C George Priya Doss Ramya Iyadurai Sowmya Satyendra Camilla Rodrigues Sangeeta Joshi Indranil Roy Bhaskar Narayan Chaudhuri DS Chitnis Dhole Tapan Balaji Veeraraghavan Source Type: research

Role of insertion sequence element is256 as a virulence marker and its association with biofilm formation among methicillin-resistant Staphylococcus epidermidis from hospital and community settings in Chennai, South India
The objective of this study was to detect the association of biofilm formation with IS256 among clinical and carrier isolates of methicillin-resistant Staphylococcus epidermidis (MRSE). A total of 71 MRSE isolates were included in this study. Phenotypic detection of biofilm formation was done by Congo red agar method. Detection of genes associated with biofilm formation (icaAD, aap and atlE) and insertion sequence IS256 was done by polymerase chain reaction. Of the 71 MRSE isolates,19/40 (47.5%) clinical isolates from hospital settings and 11/31 (35.5%) carrier isolates from community settings respectively were fou...
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Saravanan Murugesan Stalin Mani Indhumathy Kuppusamy Padma Krishnan Source Type: research

Foetomaternal outcomes of hepatitis E infection outbreak in North India
Neha Gautam, Shriya Ganju, Sunite A Ganju, Sohini Walia, Anil Kanga KumarIndian Journal of Medical Microbiology 2018 36(1):121-123Hepatitis E infection (HEV) in pregnant females, especially in the third trimester is associated with poor foetomaternal outcomes. However, the mechanisms of severe liver injury remain obscure. In a recent HEV outbreak in North India, six pregnant females were detected to be positive for HEV infection with concomitant hepatitis A infection in three pregnant females. None of the pregnant females were positive for hepatitis B or hepatitis C infection. The mortality was 50% in pregnant females....
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Neha Gautam Shriya Ganju Sunite A Ganju Sohini Walia Anil Kanga Kumar Source Type: research

In vitro analysis of the minimal inhibitory concentration values of different generations of anti-methicillin-resistant Staphylococcus aureus antibiotics
The objective of the study was to analyse in vitro MRSA susceptibility to vancomycin, linezolid, daptomycin, tigecycline, ceftaroline, dalbavancin, clindamycin, ciprofloxacin and trimethoprim/sulfamethoxazole. All MRSA strains isolated from hospitalised patients were analysed according to the current microbiological recommendations. Finally, a total of 124 MRSA strains were analysed; all were susceptible to tested antibiotics. Dalbavancin had the lowest minimum inhibitory concentration (MIC), and vancomycin the highest MIC value. There were 28/124 strains of MRSA susceptible for clindamycin, 36/124 for ciprofloxacin and 12...
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Aneta Guzek Zbigniew Rybicki Dariusz Tomaszewski Source Type: research

Survey of probiotic preparations and labeling practices in Indian market
Vikas Chandrashekhar Ghattargi, Swapnil H Kalam, Sumeet S Pandit, Shrikant P Pawar, Yogesh S ShoucheIndian Journal of Medical Microbiology 2018 36(1):116-118A cross-sectional survey was conducted across 320 chemists shop in Pune city for their availability and labeling practices. The questionnaire revealed the data about the most sold probiotic preparations, their mode of sale, and their available forms such as tablet, capsule, and sachet. Top ten probiotic preparations were evaluated for labeling practice as per the existing regulations of the Indian Council of Medical Research-Department of Biotechnology, Indian guidelin...
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Vikas Chandrashekhar Ghattargi Swapnil H Kalam Sumeet S Pandit Shrikant P Pawar Yogesh S Shouche Source Type: research

Molecular epidemiology of circulating human adenovirus types in acute conjunctivitis cases in Chandigarh, North India
Mini P Singh, Jagat Ram, Archit Kumar, Tripti Rungta, Amit Gupta, Jasmine Khurana, Radha Kanta RathoIndian Journal of Medical Microbiology 2018 36(1):113-115Human adenovirus (HAdV) is a major cause of viral conjunctivitis. The various serotypes implicated in the causation are 3, 4, 8, 19 and 37. The present study aimed to know the circulating types of HAdV causing acute conjunctivitis in North India. A total of 23 conjunctival swabs were collected from patients with clinically suspected acute viral conjunctivitis during 2014–2015. The HAdV was implicated in the etiology in 65.2% of cases. The sequencing of repres...
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Mini P Singh Jagat Ram Archit Kumar Tripti Rungta Amit Gupta Jasmine Khurana Radha Kanta Ratho Source Type: research

Diagnostic performance of serological tests to detect antibodies against acute scrub typhus infection in central India
Conclusion: IgM ELISA being simple and affordable could be an alternative diagnostic test to IgM IFA which is subjective and costly. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Kiran Pote Rahul Narang Pradeep Deshmukh Source Type: research

Association of glycerol kinase gene with class 3 integrons: A novel cassette array within Escherichia coli
Conclusion: This study provides epidemiological profiling of Class 3 integrons in this geographical area. The data generated in this study are helpful in infection control programme, anti-infective research and search for epidemiological markers. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Rajkumari Elizabeth Debadatta Dhar Chanda Atanu Chakravarty Deepjyoti Paul Shiela Chetri Deepshikha Bhowmik Jayalaxmi Wangkheimayum Amitabha Bhattacharjee Source Type: research

Methicillin-resistant Staphylococcus aureus intracranial abscess: An analytical series and review on molecular, surgical and medical aspects
Conclusion: We propose that timely diagnosis, surgical intervention and appropriate anti-MRSA treatment would contribute to better outcome. The occurrence of CA-MRSA and LA-MRSA infection in the central nervous system signifies the threat from the community and livestock reservoir, thus drawing attention towards surveillance and tracking to understand the epidemiology and implement infection control measures. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Veena Kumari Haradara Bahubali Priya Vijayan Vasundhra Bhandari Nagarathna Siddaiah Dwarakanath Srinivas Source Type: research

Utility of GeneXpert in the diagnosis, reliance on urine microscopy and clinical characteristics of genitourinary tuberculosis at a tertiary care hospital
Conclusion: The sample size in the present study is small to arrive at a brisk inference, but it may safely be postulated that yield of detection for GeneXpert may be improved using multiple sampling, especially the early morning ones. It is also pertinent to mention here that GeneXpert may not be able to pick up mutant genomes. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Ajaz Nabi Koul Basharat A Kassana Aadil Rafeeq Rather Source Type: research

Echinocandins: Their role in the management of Candida biofilms
Subramanian Swaminathan, Shweta Kamat, Nalini Adele PintoIndian Journal of Medical Microbiology 2018 36(1):87-92The importance of antifungal agents and their clinical implications has received little attention in comparison to antibiotics, particularly in the health-care setting. However, apart from bacterial infections rising in hospitals, the incidences of fungal infections are growing with the development of resistance to conventional antifungal agents. Newer antifungal agents such as echinocandins (ECs) have been extensively studied over the past decade and are recognised as a superior treatment compared with prior ant...
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Subramanian Swaminathan Shweta Kamat Nalini Adele Pinto Source Type: research

Identification of multiple strains of Porphyromonas gingivalis using heteroduplex polymerase chain reaction in varying severity of chronic periodontitis
Conclusions: Species-specific PCR and heteroduplex analysis provide a simple and accurate method to analyse the strain diversity of P. gingivalis. P. gingivalis was detected in both healthy periodontal sites as well as sites with periodontitis. The presence of two or three P. gingivalis strains was seen in 60% of the samples. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Mihir Raghavendra Kulkarni Kishore G Bhat Betsy Sara Thomas G Subraya Bhat Raghavendra Dhirendra Kulkarni Source Type: research

Comparison of different methods of RNA preparation from peripheral blood for nucleic acid amplification assay
Conclusions: These results demonstrate that despite the lower purity and integrity of RNA, our RNA preparation protocol is simple and rapid and shows reasonable performance in RT-LAMP. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Dong Eun Lee Hanna Lee Seon Duk Lee Hyung Soo Han Jae Young Choe Sora Yun Hong-Jun Park Jungbae Park Jong Kun Kim Source Type: research

Changing trends of culture-positive typhoid fever and antimicrobial susceptibility in a tertiary care North Indian Hospital over the last decade
Conclusion: We found that the most common etiological agent of enteric fever is S. Typhi causing the majority of cases from July to October in our region. MIC to ceftriaxone in typhoidal salmonellae is creeping towards resistance and more data are needed to understand the azithromycin susceptibility. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Priyanka Sharma Sushila Dahiya Neelam Manral Bhavana Kumari Sambuddha Kumar Sangeeta Pandey Seema Sood Bimal Kumar Das Arti Kapil Source Type: research

Nonspecific amplification of human DNA by Streptococcus pneumoniae LytA primer
Conclusion: Since HRMA is done without a specific probe, one should be aware of non-specific amplifications while using primers for HRMA of human clinical samples. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Helen Hencida Thangamony Ravindran Kumar Chinnappan Palaniappan Thangavelu Mani Mariappa Berlin Grace Viswanathan Mariammal Kootallur Narayanan Brahmadathan Source Type: research