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Condition: Pain

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Total 374 results found since Jan 2013.

Employing the sustained-release properties of poly(lactic-co-glycolic acid) nanoparticles to reveal a novel mechanism of sodium-hydrogen exchanger 1 in neuropathic pain
Transl Res. 2023 Sep 5:S1931-5244(23)00143-3. doi: 10.1016/j.trsl.2023.09.003. Online ahead of print.ABSTRACTNeuropathic pain is caused by injury or disease of the somatosensory system, and its course is usually chronic. Several studies have been dedicated to investigating neuropathic pain-related targets; however, little attention has been paid to the persistent alterations that these targets, some of which may be crucial to the pathophysiology of neuropathic pain. The present study aimed to identify potential targets that may play a crucial role in neuropathic pain and validate their long-term impact. Through bioinformat...
Source: Translational Research : the journal of laboratory and clinical medicine - September 7, 2023 Category: Laboratory Medicine Authors: Junhua Wu Meiling Jin Quangdon Tran Minwoo Kim Song I Kim Juhee Shin Hyewon Park Nara Shin Hyunji Kang Hyo Jung Shin Sun Yeul Lee Song-Biao Cui C Justin Lee Won Hyung Lee Dong Woon Kim Source Type: research

Rab7 Silencing Prevents μ-Opioid Receptor Lysosomal Targeting and Rescues Opioid Responsiveness to Strengthen Diabetic Neuropathic Pain Therapy.
This study investigated whether enhanced Rab7-mediated lysosomal targeting of peripheral sensory neuron μ-opioid receptors (MORs) is responsible for diminished opioid responsiveness in rats with streptozotocin-induced diabetes. In diabetic animals, significantly impaired peripheral opioid analgesia was associated with a loss in sensory neuron MOR and a reduction in functional MOR G-protein-coupling. In control animals, MORs were retained mainly on the neuronal cell membrane. In contrast, in diabetic rats, they were colocalized with upregulated Rab7 in LampI-positive perinuclear lysosome compartments. Silencing endogenous ...
Source: Diabetes - December 10, 2012 Category: Endocrinology Authors: Mousa SA, Shaqura M, Khalefa BI, Zöllner C, Schaad L, Schneider J, Shippenberg TS, Richter JF, Hellweg R, Shakibaei M, Schäfer M Tags: Diabetes Source Type: research

Willow bark extract increases antioxidant enzymes and reduces oxidative stress through activation of Nrf2 in vascular endothelial cells and Caenorhabditis elegans.
This study investigated the antioxidative effects of WBE in human umbilical vein endothelial cells (HUVECs) and Caenorhabditis elegans. WBE prevented oxidative-stress-induced cytotoxicity of HUVECs and death of C. elegans. WBE dose-dependently increased mRNA and protein expression levels of the nuclear factor erythroid 2-related factor 2 (Nrf2) target genes heme oxygenase-1, γ-glutamylcysteine ligase modifier and catalytic subunits, and p62 and intracellular glutathione (GSH) in HUVECs. In the nematode C. elegans, WBE increased the expression of the gcs-1::green fluorescent protein reporter, a well-characterized target of...
Source: Free Radical Biology and Medicine - December 28, 2012 Category: Biology Authors: Ishikado A, Sono Y, Matsumoto M, Robida-Stubbs S, Okuno A, Goto M, King GL, Keith Blackwell T, Makino T Tags: Free Radic Biol Med Source Type: research

The expression and functionality of stromal caveolin 1 in human adenomyosis
This study assessed the functional role of stromal CAV1 and RANTES in a small number of human adenomyosis samples by immunohistochemistry and in primary human ESCs by functional studies. In future investigations, a larger sample size should be adopted and the functional role of stromal CAV1 should be further characterized in animal models. WIDER IMPLICATIONS OF THE FINDINGS Loss of stromal CAV1 expression may play a critical role in the pathogenesis of adenomyosis and is correlated with adenomyosis-related dysmenorrhea. STUDY FUNDING National Basic Research Program of China and Ph.D. Programs Foundation of Ministry of Ed...
Source: Human Reproduction - April 16, 2013 Category: Reproduction Medicine Authors: Zhao, L., Zhou, S., Zou, L., Zhao, X. Tags: Reproductive biology Source Type: research

Phospholipase C-related but catalytically inactive protein modulates pain behavior in a neuropathic pain model in mice
Conclusions: Suppressed expression of PRIPs induces an elevated expression of KCC2 in the spinal cord, resulting in inhibition of nociception and amelioration of neuropathic pain in DKO mice.
Source: Molecular Pain - May 2, 2013 Category: Molecular Biology Authors: Tomoya KitayamaKatsuya MoritaRizia SultanaNami KikushigeKeisuke MgitaShinya UenoMasato HirataTakashi Kanematsu Source Type: research

Transport of gabapentin by LAT1 (SLC7A5).
Abstract Gabapentin is used in the treatment of epilepsy and neuropathic pain. Gabapentin has high and saturable permeability across the BBB, but no mechanistic studies underpinning this process have been reported. The aim of the current study was to investigate the transport of gabapentin in a model of the BBB, identify the important drug transporter(s) and to use mathematical modelling to quantify the processes involved. A human brain endothelial cell line (hCMEC/D3) was utilised as an in-vitro model of the BBB. Uptake of radiolabeled gabapentin into cells in the presence of chemical inhibitors, siRNA or overexp...
Source: Biochemical Pharmacology - May 25, 2013 Category: Drugs & Pharmacology Authors: Dickens D, Webb SD, Antonyuk S, Giannoudis A, Owen A, Rädisch S, Hasnain SS, Pirmohamed M Tags: Biochem Pharmacol Source Type: research

Down-Regulation of Annexin A1 in the Urothelium Decreases Cell Survival After Bacterial Toxin Exposure
Conclusions: Several annexins are expressed in human bladder and TEU-2 cells, in which levels are regulated during urothelial differentiation. Annexin A1 down-regulation in patients with bladder pain syndrome might decrease cell survival and contribute to compromised urothelial function.
Source: The Journal of Urology - February 1, 2013 Category: Urology & Nephrology Authors: Katia Monastyrskaya, Eduard B. Babiychuk, Annette Draeger, Fiona C. Burkhard Tags: Investigative Urology Source Type: research

Knockdown of sodium channel NaV1.6 blocks mechanical pain and abnormal bursting activity of afferent neurons in inflamed sensory ganglia
Summary: Knocking down expression of the sodium channel isoform NaV1.6 in lumbar sensory ganglia blocked development of pain behaviors and neuronal hyperexcitability induced by local inflammation of the ganglion.Abstract: Inflammatory processes in the sensory ganglia contribute to many forms of chronic pain. We previously showed that local inflammation of the lumbar sensory ganglia rapidly leads to prolonged mechanical pain behaviors and high levels of spontaneous bursting activity in myelinated cells. Abnormal spontaneous activity of sensory neurons occurs early in many preclinical pain models and initiates many other pat...
Source: Pain - March 7, 2013 Category: Anesthesiology Authors: Wenrui Xie, Judith A. Strong, Ling Ye, Ju-Xian Mao, Jun-Ming Zhang Tags: Research papers Source Type: research

Full-length membrane-bound tumor necrosis factor-α acts through tumor necrosis factor receptor 2 to modify phenotype of sensory neurons
Summary: Membrane-bound tumor necrosis factor-α, acting through tumor necrosis factor receptor 2, causes increased expression in dorsal root ganglia of genes whose products are implicated in heightened neuronal excitability characteristic of chronic neuropathic pain.Abstract: Neuropathic pain resulting from spinal hemisection or selective spinal nerve ligation is characterized by an increase in membrane-bound tumor necrosis factor-alpha (mTNFα) in spinal microglia without detectable release of soluble TNFα (sTNFα). In tissue culture, we showed that a full-length transmembrane cleavage-resistant TNFα (CRTNFα) construc...
Source: Pain - May 28, 2013 Category: Anesthesiology Authors: Zetang Wu, Shiyong Wang, Sandy Gruber, Marina Mata, David J. Fink Tags: Research papers Source Type: research

G protein‐gated inwardly rectifying potassium (GIRK) channels play a primary role in the antinociceptive effect of oxycodone, but not morphine, at supraspinal sites
Conclusion and ImplicationsThe results demonstrated that GIRK1 channels play a primary role in the antinociceptive effects of oxycodone, but not morphine, at supraspinal sites, and suggested that supraspinal GIRK1 channels are responsible for the unique analgesic profile of oxycodone.
Source: British Journal of Pharmacology - October 7, 2013 Category: Drugs & Pharmacology Authors: Atsushi Nakamura, Masahide Fujita, Hiroko Ono, Yoshie Hongo, Tomoe Kanbara, Koichi Ogawa, Yasuhide Morioka, Atsushi Nishiyori, Masahiro Shibasaki, Tomohisa Mori, Tsutomu Suzuki, Gaku Sakaguchi, Akira Kato, Minoru Hasegawa Tags: Research Paper Source Type: research

P2X3 Receptor and Inflammatory Nociception
Conclusions: Nociceptive response intensity was measured by observing the rat's behavior and considering the number of times the animal reflexively raised its hind paw (flinches) in 60 min. Local subcutaneous administration of the selective TRPA1, 5-HT3 or 5-HT1A receptor antagonists HC 030031, tropisetron and WAY 100,135, respectively, prevented the nociceptive responses induced by the administration in the same site of the non-selective P2X3 receptor agonist αβmeATP. Administration of the selective P2X3 and P2X2/3 receptor antagonist A-317491 or pretreatment with oligonucleotides antisense against P2X3 receptor prevent...
Source: Neuromics - October 12, 2013 Category: Neuroscience Tags: Purinergic Receptor Antibodies inflammatory nociception Western Blot Nociceptors Pain Research P2X3 Receptor Antibody WB P2X3 Antibody Western Blotting Source Type: news

The progressive ankylosis gene product ANK regulates extracellular ATP levels in primary articular chondrocytes
IntroductionExtracellular ATP (eATP) is released by articular chondrocytes under physiological and pathological conditions. High eATP levels cause pathologic calcification, damage cartilage, and mediate pain. We recently showed that stable over-expression of the progressive ankylosis gene product, ANK, increased chondrocyte eATP levels, but the mechanisms of this effect remained unexplored. The purpose of this work was to further investigate mechanisms of eATP efflux in primary articular chondrocytes and to better define the role of ANK in this process. Methods: We measured eATP levels using a bioluminescence-based assay i...
Source: Arthritis Research and Therapy - October 17, 2013 Category: Rheumatology Authors: Ann RosenthalClaudia GohrElizabeth Mitton-FitzgeraldMegan LutzGeorge DubyakLawrence Ryan Source Type: research

Gelsemine, a principal alkaloid from Gelsemium sempervirens Ait., exhibits potent and specific antinociception in chronic pain by acting at spinal α3 glycine receptors
TOC summary: Gelsemine produces potent and specific antinociception in chronic pain states by activating spinal α3 glycine receptors without inducing tolerance.Abstract: The present study examined the antinociceptive effects of gelsemine, the principal alkaloid in Gelsemium sempervirens Ait. A single intrathecal injection of gelsemine produced potent and specific antinociception in formalin-induced tonic pain, bone cancer-induced mechanical allodynia, and spinal nerve ligation-induced painful neuropathy. The antinociception was dose-dependent, with maximal inhibition of 50% to 60% and ED50 values of 0.5 to 0.6μg. Multipl...
Source: Pain - July 24, 2013 Category: Anesthesiology Authors: Jing-Yang Zhang, Nian Gong, Jin-Lu Huang, Ling-Chen Guo, Yong-Xiang Wang Tags: Research papers Source Type: research

Identification of a Receptor for VGF Molecular Bases of Disease
VGF (nonacronymic) is a neuropeptide precursor that plays multiple roles in regulation of energy balance, reproduction, hippocampal synaptic plasticity, and pain. Data from a number of pain models showed significant up-regulation of VGF in sensory neurons. TLQP-21, one of the VGF-derived neuropeptides, has been shown to induce a hyperalgesic response when injected subcutaneously into the hind paw of mice. However, the precise role of VGF-derived neuropeptides in neuropathic pain and the molecular identity of the receptor for VGF-derived peptides are yet to be investigated. Here we identified gC1qR, the globular heads of th...
Source: Journal of Biological Chemistry - November 29, 2013 Category: Chemistry Authors: Chen, Y.–C., Pristera, A., Ayub, M., Swanwick, R. S., Karu, K., Hamada, Y., Rice, A. S. C., Okuse, K. Tags: Neurobiology Source Type: research

Heterologous Regulation of Agonist‐Independent μ‐Opioid Receptor Phosphorylation by Protein Kinase C
Conclusions & ImplicationsThe present results unravel novel mechanisms of heterologous regulation of MOR phosphorylation by PKC. These findings represent a useful starting point for definitive experiments elucidating the exact contribution of PKC‐driven MOR phosphorylation to diminished MOR responsiveness in morphine tolerance and pathological pain.
Source: British Journal of Pharmacology - December 6, 2013 Category: Drugs & Pharmacology Authors: Susann Illing, Anika Mann, Stefan Schulz Tags: Research Paper Source Type: research