Filtered By:
Condition: Pain

This page shows you your search results in order of relevance.

Order by Relevance | Date

Total 374 results found since Jan 2013.

i-Fect ™ Delivers you siRNA Payload
Delivering siRNA to Dorsal Root Ganglia to Silence K V Receptors. Our i-Fect transfection kits continue to be used to optimize delivery in vivo and into hard to transfect cells like primary neurons . In these 2 latest expamples, researchers use i-Fect to deliver siRNA to K V Receptors in Rat DRGs. Knocking down these receptors enable the study of their role in pain modulation: John H. Winston, Sushil K. Sarna. Developmental Origins of Functional Dyspepsia-Like Gastric Hypersensitivity in Rats. Gastroenterology. Volume 144, Issue 3, March 2013, Pages 570 –579.e3. dx.doi.org/10.1053/j.gastro.2012.11.001....intrathecal...
Source: siRNA and DsiRNA Transfection Efficiency - March 16, 2013 Category: Neuroscience Tags: Delivering siRNA to the CNS Dorsal root ganglia i-Fect in vivo siRNA intrathecal delivery of siRNA KV Receptors kv1.1 Potassium Channel Subunit Kv9.1 Potassium Channel Subunit Transfection Kits Source Type: news

i-Fect™ Delivers you siRNA Payload
Delivering siRNA to Dorsal Root Ganglia to Silence KV Receptors.Our i-Fect transfection kits continue to be used to optimize delivery in vivo and into hard to transfect cells like primary neurons. In these 2 latest expamples, researchers use i-Fect to deliver siRNA to KV Receptors in Rat DRGs. Knocking down these receptors enable the study of their role in pain modulation: John H. Winston, Sushil K. Sarna. Developmental Origins of Functional Dyspepsia-Like Gastric Hypersensitivity in Rats. Gastroenterology. Volume 144, Issue 3, March 2013, Pages 570–579.e3. dx.doi.org/10.1053/j.gastro.2012.11.001....intrathecal treatment...
Source: siRNA and DsiRNA Transfection Efficiency - March 16, 2013 Category: Neuroscience Tags: Kv9.1 Potassium Channel Subunit kv1.1 Potassium Channel Subunit Delivering siRNA to the CNS Transfection Kits KV Receptors i-Fect Dorsal root ganglia intrathecal delivery of siRNA in vivo siRNA Source Type: news

i-Fect siRNA Transfection Successes
Meeting your Gene Expression Analysis Requirements.I have posted many success stories resulting from use of  Neuromics'  Neuromics' i-FectTM siRNA Transfection Kit. It has been 3 months since my last posting so here I would like to post some recent highlights. Here I feature customer publication demonstrating:Down regulation of Neuroligin 2-data showed unexpected upregulation and pronociceptive effects of the “inhibitory” NL2 in neuropathic pain, suggesting a functional shift of NL2 from inhibition to excitation that changed the synaptic ratio toward higher excitation.Role of IGFPB5 for survival of neurons in...
Source: siRNA and DsiRNA Transfection Efficiency - July 29, 2013 Category: Neuroscience Tags: Neuroligin 2 Gene berrant Histone Deacetylase2 gene IGFBP5 gene NL2 intraventicular delivery of siRNA ADAC2 i-Fect PSD95 intrathecal delivery of siRNA in vivo siRNA Source Type: news

Delivery of siRNA, miRNA, shRNA and Plasmids Guaranteed
Potent and Frequently Published Transfection Solutions We have proven and frequently published transfection solutions. Powerful punch and versatility are now needed more than ever with the hyper growth in gene manipulation technologies like Sleeping BeautyTM and CRISPR-Cas9.We have published examples of the use of our solutions for delivering siRNA, miRNA, shRNA and plasmids both in vitro and in vivo. Here are some of your options. i-Fect ™ -A novel cationic lipid formulation specifically designed for efficient delivery of 27mer DsiRNAs(dicer substrate small Interfering RNAs)& 21mer siRNAs (small interfering RNA...
Source: siRNA and DsiRNA Transfection Efficiency - January 20, 2016 Category: Neuroscience Tags: DNA transfection i-Fect miRNA Delivery Methods p-fect Plasmid Transfection plasmids pn-Fect shRNA siRNA Source Type: news

Delivering siRNA in vivo
New Publications Ouri-Fect transfection kit was one of the first products. This has resulted in it being widely used andfrequently published.We kick off 2018 with some new publications. Here we feature a study where researchers use our kit to deliver G-protein-coupled receptor C (MrgprC) siRNA in vivo. This receptor is part of the TRPV1 pathway.Ying-jun Liu, Xiao-xi Lin, Jian-qiao Fang, and Fang Fang. Involvement of MrgprC in electroacupuncture analgesia for attenuating CFA-induced thermal hyperalgesia by suppressing the TRPV1 pathway. Pre-pub-full text PDFImage: Expression of TRPV1 in rats treated with MrgrprC s...
Source: siRNA and DsiRNA Transfection Efficiency - January 13, 2018 Category: Neuroscience Tags: inflammatory pain intrathecal delivery of siRNA Mas-related G-protein-coupled receptor C MrgrpfC TRPV1 Source Type: news

i-Fect™ Delivers Your siRNA Payload
Delivering siRNA to Dorsal Root Ganglia to Silence KV Receptors.Our i-Fect transfection kits continue to be used to optimize delivery in vivo and into hard to transfect cells like primary neurons. In these 2 latest expamples, researchers use i-Fect to deliver siRNA to KV Receptors in Rat DRGs. Knocking down these receptors enable the study of their role in pain modulation: John H. Winston, Sushil K. Sarna. Developmental Origins of Functional Dyspepsia-Like Gastric Hypersensitivity in Rats. Gastroenterology. Volume 144, Issue 3, March 2013, Pages 570–579.e3. dx.doi.org/10.1053/j.gastro.2012.11.001....intrathecal treatment...
Source: Neuromics - March 16, 2013 Category: Neuroscience Tags: siRNA delivery in-vivo Delivering siRNA to the CNS KV receptors. i-Fect Kv9.1 Potassium Channel Source Type: news

Epigenetics and Pain Research
i-Fect Used to Study ImpactsOur i-Fect siRNA, miRNA and shRNA Trasfection Kit was recently used to study the impact of G9a-specific siRNA (AGUAACGGGCAUCAAUGC) on Mu Opioid Receptors: Yuhao Zhang, Shao-Rui Chen, Geoffroy Laumet, Hong Chen and Hui-Lin Pan. Nerve Injury Diminishes Opioid Analgesia through Lysine Methyltransferase-Mediated Transcriptional Repression of µ-Opioid Receptors in Primary Sensory Neurons. First Published on February 25, 2016, doi: 10.1074/jbc.M115.711812... In some SNL rats, G9a-specific siRNA (4 µg) or the negative control siRNA was administered intrathecally. G9a-specific siRNA(AGUAACGGGCAUC...
Source: siRNA and DsiRNA Transfection Efficiency - March 24, 2016 Category: Neuroscience Tags: epigenetics Ga9 Mu Opioid Receptor inhibitor i-Fect iFect intrathecal delivery of siRNA Morphine neuropathic pain Source Type: news

Silencing Cytokines in-vivo with i-Fect
Knocking Down Cytokine to Study Pain ResponseWe have many unique applications published from researchers using our Transfection Kits in vitro and in vivo. Here researchers simultaneously 3 immune/inflammatory response cytokines in vivo: Byung Moon Choi, Soo Han Lee, Sang Mee An, Do Yang Park, Gwan Woo Lee, and Gyu-Jeong Noh. The time-course and RNA interference of TNF-α, IL-6, and IL-1β expression on neuropathic pain induced by L5 spinal nerve transection in rats. Korean J Anesthesiol. 2015 Apr;68(2):159-169. English. Published online March 30, 2015. http://dx.doi.org/10.4097/kjae.2015.68.2.159.Protocol: RNAs w...
Source: siRNA and DsiRNA Transfection Efficiency - April 7, 2015 Category: Neuroscience Tags: allodynia analgesia Gene Expression Analysis Gene Silencing i-Fect IL-1B IL-6 inflammatory pain intrathecal delivery of siRNA TNF-alpha Transfection Kits Source Type: news

i-Fect in Action
This study is the first to demonstrate that the stabilization of HIF1A expression underpins the development of bortezomib-induced neuropathic pain. Crucially, these findings reveal that the initiation and maintenance of bortezomib-induced neuropathic pain are regulated by distinct mechanisms.Looking to up your odds for high percentage siRNA Transfection? Try i-Fect.
Source: siRNA and DsiRNA Transfection Efficiency - May 9, 2019 Category: Neuroscience Tags: Chemotherapy i-Fect in vivo RNAi neuropathic pain siRNA delivery in-vivo Source Type: news

Using i-Fect to Knock Down GLT-1 Gene in vivo
Pain Researchers continue to use our i-FectTM Transfection Kit for Gene Expression AnalysisValproate Prevents Dysregulation of Spinal Glutamate and Reduces the Development of Hypersensitivity in Rats After Peripheral Nerve Injury. The Journal of Pain, Volume 14, Issue 11, November 2013, Pages 1485–1491 http://dx.doi.org/10.1016/j.jpain.2013.07.007.Researchers use the kit to deliver Glutamate Receptor 1 siRNA in vivo.The present study examined whether the histone deacetylase inhibitor valproate prevents downregulation of glutamate transporters in the primary cultured astrocytes and in the spinal cord after L5-L6 spinal ne...
Source: siRNA and DsiRNA Transfection Efficiency - November 6, 2013 Category: Neuroscience Tags: Delivering siRNA to the CNS GLT-1 Gene Glutamate Receptor 1 siRNA in vivo i-Fect intrathecal delivery of siRNA Source Type: news

Neuromics' Transfection Kits-Genes Studied
Delivering siRNA, miRNA, Plasmids and Viral Vectors for Gene Expression Analysis.I have shared the many genes researchers have studied using our Transfection Kits. These include: β-arrestin, ABCA, ASIC, β-arrestin, CAV1.2, CX3CR1, DOR, EHDAC2, LOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 , neuroligin 2, The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK, SNSR1, hTert, NOV, Survivin, TLR4, Troy and TRPV1 and More!We can now add GPNMB to this list: Lili Hou, Yanfeng Zhang, Yong Yang, Kai Xiang, Qindong Tan, Qulian Guo. Intrathecal siRNA Against GPNMB Attenuates Nociception in a Rat Model of Neuropath...
Source: siRNA and DsiRNA Transfection Efficiency - July 23, 2014 Category: Neuroscience Tags: Delivering siRNA to the CNS Gene Expression Analysis Gene Silencing i-Fect in vivo siRNA pn-Fect tansfection Transfection Kits Source Type: news

Neuromics ' Transfection Kits-Genes Studied
Delivering siRNA, miRNA, Plasmids and Viral Vectors  for Gene Expression Analysis . I have shared the many genes researchers have studied using our Transfection Kits . These include: β-arrestin, ABCA, ASIC, β-arrestin, CAV1.2, CX3CR1, DOR, EHDAC2, LOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 , neuroligin 2, The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK, SNSR1, hTert, NOV, Survivin, TLR4, Troy and TRPV1 and More! We can now add GPNMB to this list: Lili Hou, Yanfeng Zhang, Yong Yang, Kai Xiang, Qindong Tan, Qulian Guo. Intrathecal siRNA Against GPNMB Attenuates Nociception in a Rat Model of N...
Source: siRNA and DsiRNA Transfection Efficiency - July 23, 2014 Category: Neuroscience Tags: Delivering siRNA to the CNS Gene Expression Analysis Gene Silencing i-Fect in vivo siRNA pn-Fect tansfection Transfection Kits Source Type: news

i-Fect, BDNF and Irritable Bowel Syndrome (IBS)
Conclusion: Chronic prenatal stress followed by a second exposure to HeICS in adult offspring exacerbated visceral hypersensitivity (VHS) greater in female offspring that persisted longer than in male offspring. Chronic prenatal stress up-regulated BDNF expression in the lumbar-sacral dorsal horn that correlated with the exacerbation of VHS in female, but not in male offspring by increasing RNA Pol II binding and histone H3 acetylation, and decreasing histone deacetylase 1 association with the core promoter of BDNF in female offspring.
Source: siRNA and DsiRNA Transfection Efficiency - March 11, 2014 Category: Neuroscience Tags: BDNF;chronic stress;functional bowel disorders;iritable bowel syndrome;visceral hypersnsitivity i-Fect intrathecal delivery of siRNA Source Type: news

Water-Soluble Polymer Assists N-Methyl-D-Aspartic Acid Receptor 2B siRNA Delivery to Relieve Chronic Inflammatory Pain In Vitro and In Vivo.
Abstract We constructed a water-soluble lipopolymer (WSLP) as a nonviral gene carrier to deliver siRNA targeting NR2B. The cytotoxicity and serum stability of WSLP loaded with siRNA were evaluated, and the knockdown efficiency of WSLP/NR2B-siRNA in PC12 cells was examined. The results showed that WSLP could protect the loading siRNAs from enzymatic degradation in serum and exhibit low cytotoxicity to cells. After transfection, WSLP/NR2B-siRNA complexes reduced the NR2B transcriptional level by 50% and protein level by 55% compared to control siRNA. Moreover, 3 days after intrathecal injection of WSLP/NR2B-siRNA co...
Source: Pain Research and Management - April 8, 2018 Category: Anesthesiology Authors: Peng J, Ma J, Yang X, He H, Wu H, Ma T, Lu J Tags: Pain Res Manag Source Type: research

Intrathecal injection of phosphodiesterase 4B-specific siRNA attenuates neuropathic pain in rats with L5 spinal nerve ligation.
Authors: Ji Q, Di Y, He X, Liu Q, Liu J, Li W, Zhang L Abstract Phosphodiesterase 4 (PDE4) is an adenosine cyclic 3,5-monophosphate-specific degradative enzyme, which is closely associated with the inflammatory response. Among its four subtypes (A‑D), it remains unclear which one exerts suppressive effects on inflammation and reduces neuropathic pain. The present study aimed to examine the modulation of neuroinflammation by PDE4 subtypes in the spinal cord of a rat model of L5 spinal nerve ligation (SNL)‑induced neuropathic pain. The expression levels of PDE4A‑D were measured in the lumbar spinal cords of na...
Source: Molecular Medicine Reports - December 29, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research