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Specialty: Biomedical Science
Condition: Diabetes

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Total 31 results found since Jan 2013.

MIC26 and MIC27 are bona fide subunits of the MICOS complex in mitochondria and do not exist as glycosylated apolipoproteins
by Melissa Lubeck, Nick H. Derkum, Ritam Naha, Rebecca Strohm, Marc D. Driessen, Bengt-Frederik Belgardt, Michael Roden, Kai St ühler, Ruchika Anand, Andreas S. Reichert, Arun Kumar Kondadi Impairments of mitochondrial functions are linked to human ageing and pathologies such as cancer, cardiomyopathy, neurodegeneration and diabetes. Specifically, aberrations in ultrastructure of mitochondrial inner membrane (IM) and factors regulating them are linked to diabetes. The development of diabetes is connected to the ‘Mitochondrial Contact Site and Cristae Organising System’ (MICOS) complex which is a large membrane protei...
Source: PLoS One - June 6, 2023 Category: Biomedical Science Authors: Melissa Lubeck Source Type: research

Long noncoding RNA Meg3 mediates ferroptosis induced by oxygen and glucose deprivation combined with hyperglycemia in rat brain microvascular endothelial cells, through modulating the p53/GPX4 axis
In this study, rat brain microvascular endothelial cells (RBMVECs) were exposed to 6 h of oxygen and glucose deprivation (OGD), and subsequent reperfusionvia incubating cells with glucose of various high concentrations for 24 h to imitateinvitro diabetic brain ischemic injury. It was shown that the marker events of ferroptosis and increasedMeg3 expression occurred after the injury induced by OGD combined with hyperglycemia. However, all ferroptotic events were reversed with the treatment ofMeg3-siRNA. Moreover, in this in vitro model, p53 was also characterized as a downstream target ofMeg3. Furthermore, p53 knockdown prot...
Source: European Journal of Histochemistry - July 2, 2021 Category: Biomedical Science Authors: Cheng Chen, Yan Huang, Pingping Xia, Fan Zhang, Longyan Li, E Wang, Qulian Guo, Zhi Ye Source Type: research

Melatonin protects against focal cerebral ischemia-reperfusion injury in diabetic mice by ameliorating mitochondrial impairments: involvement of the Akt-SIRT3-SOD2 signaling pathway
Aging (Albany NY). 2021 Jun 11;13. doi: 10.18632/aging.203137. Online ahead of print.ABSTRACTDiabetic patients are more vulnerable to cerebral ischemia-reperfusion (CIR) injury and have a worse prognosis and higher mortality after ischemic stroke than non-diabetic counterparts. Melatonin can exert neuroprotective effects against CIR injury in nondiabetic animal models. However, its effects on diabetic CIR injury and the underlying mechanisms remain unclarified. Herein, we found that melatonin administration improved neurological deficit, cerebral infarct volume, brain edema, and cell viability, reduced mitochondrial swelli...
Source: Aging - June 12, 2021 Category: Biomedical Science Authors: Lian Liu Quan Cao Wenwei Gao Bingyu Li Zhongyuan Xia Bo Zhao Source Type: research

FX5 as a non-steroidal GR antagonist improved glucose homeostasis in type 2 diabetic mice via GR/HNF4 α/miR-122-5p pathway.
FX5 as a non-steroidal GR antagonist improved glucose homeostasis in type 2 diabetic mice via GR/HNF4α/miR-122-5p pathway. Aging (Albany NY). 2020 Dec 09;12: Authors: Xu X, Chen Y, Zhu D, Zhao T, Xu R, Wang J, Hu L, Shen X Abstract Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease characterized by glucose metabolic disorders, and gluconeogenesis inhibiting is a promisingly therapeutic strategy for T2DM. Glucocorticoid receptor (GR) is tightly implicated in the regulation of gluconeogenesis, although the underlying mechanism remains obscure. Here, we discovered that small molecule, 5-chlo...
Source: Aging - December 9, 2020 Category: Biomedical Science Authors: Xu X, Chen Y, Zhu D, Zhao T, Xu R, Wang J, Hu L, Shen X Tags: Aging (Albany NY) Source Type: research

miR-27-3p inhibition restore fibroblasts viability in diabetic wound by targeting NOVA1.
Abstract Diabetic wounds increase morbidity and decrease quality of life in patients with type 2 diabetes. Serum miR-27-3p levels are reportedly elevated in type 2 diabetic patients. In the present study, we explored the role of miR-27-3p during wound healing. We found that miR-27-3p is overexpressed in cutaneous fibroblasts of diabetic patients and mice. miR-27-3p knockdown enhanced the proliferation and migration of fibroblasts, while suppressing the incidence of fibroblast apoptosis. Overexpressing miR-27-3p in fibroblasts had the opposite effects. We also identified neuro-oncological ventral antigen 1 (NOVA1) ...
Source: Aging - June 25, 2020 Category: Biomedical Science Authors: Zhang P, Song X, Dong Q, Zhou L, Wang L Tags: Aging (Albany NY) Source Type: research

Attenuation of diabetic kidney injury in DPP4-deficient rats; role of GLP-1 on the suppression of AGE formation by inducing glyoxalase 1.
Abstract Dipeptidyl peptidase 4 (DPP4) inactivates incretin hormone glucagon-like peptide-1. DPP4 inhibitors may exert beneficial effects on diabetic nephropathy (DN) independently of glycemic control; however, the mechanisms underlying are not fully understood. Here, we investigated the mechanisms of the beneficial effects of DPP4 inhibition on DN using DPP4-deficient (DPP4-def) rats and rat mesangial cells.Blood glucose and HbA1c significantly increased by streptozotocin (STZ) and no differences were between WT-STZ and DPP4-def-STZ. The albumin level in urine decreased significantly and the albumin/creatinine ra...
Source: Aging - January 5, 2020 Category: Biomedical Science Authors: Sarker MK, Lee JH, Lee DH, Chun KH, Jun HS Tags: Aging (Albany NY) Source Type: research

High Glucose Provokes Microvesicles Generation from Glomerular Podocytes via NOX4/ROS Pathway.
Abstract Microvesicles (MVs) were involved in the pathogenesis of many diseases, such as cardiovascular diseases and diabetes. Oxidative stress played a key role in the development and progression of diabetic nephropathy. Our aim of this study was to investigate whether high glucose could provoke microvesicles generation from podocytes and its potential mechanism. Mouse podocyte clone 5 (MPC-5) were stimulated by high glucose. The intracellular reactive oxygen species (ROS) of podocytes were measured by fluorescence microscopy with the probe of CM-H2DCFDA and MitoSOXTM. Antioxidants N-Acetyl-L-cysteine (NAC) and ...
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Li M, Zhang T, Wu X, Chen Y, Sun L Tags: Biosci Rep Source Type: research

Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3 α.
Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3α. Biosci Rep. 2019 Oct 30;39(10): Authors: Wang Y, Gong W, Lv S, Qu H, He Y Abstract Methylation of hypoxia-inducible factor-3α (HIF3A) was previously demonstrated to be highly associated with insulin resistance (IR) in patients with gestational diabetes mellitus (GDM). We aimed to study the therapeutic effects of Berberine (BBR) on GDM and the possible mechanisms. The expressions and methylated states of HIF3A in pregnant women with GDM were compared with that in healthy controls. T...
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Wang Y, Gong W, Lv S, Qu H, He Y Tags: Biosci Rep Source Type: research

ATP-binding cassette A1 deficiency causes cardiolipin-driven mitochondrial dysfunction in podocytes
Fibroblasts from patients with Tangier disease carrying ATP-binding cassette A1 (ABCA1) loss-of-function mutations are characterized by cardiolipin accumulation, a mitochondrial-specific phospholipid. Suppression of ABCA1 expression occurs in glomeruli from patients with diabetic kidney disease (DKD) and in human podocytes exposed to DKD sera collected prior to the development of DKD. We demonstrated that siRNA ABCA1 knockdown in podocytes led to reduced oxygen consumption capabilities associated with alterations in the oxidative phosphorylation (OXPHOS) complexes and with cardiolipin accumulation. Podocyte-specific deleti...
Source: Journal of Clinical Investigation - July 23, 2019 Category: Biomedical Science Authors: G. Michelle Ducasa, Alla Mitrofanova, Shamroop K. Mallela, Xiaochen Liu, Judith Molina, Alexis Sloan, Christopher E. Pedigo, Mengyuan Ge, Javier Varona Santos, Yanio Hernandez, Jin-Ju Kim, Cyrille Maugeais, Armando J. Mendez, Viji Nair, Matthias Kretzler, Source Type: research

Inhibition of acid sphingomyelinase activity ameliorates endothelial dysfunction in db/db mice.
Abstract Acid sphingomyelinase (aSMase) plays an important role in endothelial dysfunction. Here, we show that elevated aSMase activity and ceramide content were reduced by desipramine treatment in diabetic animals. The inhibitor of aSMase, desipramine, improved vascular dysfunction in db/db mice. High glucose-induced upregulation of aSMase activity and ceramide levels were restored by treatment with aSMase siRNA or desipramine in endothelial cells. In addition, aSMase siRNA or desipramine treatment increased the release of nitric oxide (NO) and the phosphorylation of endothelial NO synthase (eNOS) in diabetic mou...
Source: Bioscience Reports - March 24, 2019 Category: Biomedical Science Authors: Jiang M, Huang S, Duan W, Liu Q, Lei M Tags: Biosci Rep Source Type: research

Carbohydrate response element binding protein (ChREBP) modulates the inflammatory response of mesangial cells in response to glucose.
Abstract Diabetic nephropathy (DN) is one of the most devastating complications of diabetes mellitus. Carbohydrate response element binding protein (ChREBP) is a basic helix-loop-helix leucine zipper transcription factor that primarily mediates glucose homeostasis in the body. The present study investigated the role of ChREBP in the pathogenesis of DN. The expression of ChREBP was detected in patients with type 2 diabetes mellitus (T2DM), diabetic mice and mesangial cells. ELISA was used to measure cytokine production in mesangial cells. Flow cytometry analysis was performed to detect the apoptosis of mes...
Source: Bioscience Reports - November 12, 2018 Category: Biomedical Science Authors: Chen Y, Wang YJ, Zhao Y, Wang JC Tags: Biosci Rep Source Type: research

Ectopic lipid accumulation: potential role in tubular injury and inflammation in diabetic kidney disease.
Emerging studies suggest that lipid accumulates in the kidneys during diabetic kidney disease (DKD). However, the correlation between ectopic lipid accumulation with tubular damage has not been thoroughly elucidated to date. Using Oil Red staining, lipid accumulation was observed in the kidneys of type 2 DKD patients (class II-III) and db/db mice compared to the control and was predominantly located in the proximal tubular compartment. Immunohistochemistry staining showed that the intensity of adipose differentiation related protein (ADRP) and sterol-regulatory element binding protein-1 (SREBP-1) was clearly upregulated, w...
Source: Clinical Science - October 22, 2018 Category: Biomedical Science Authors: Yang, W., Luo, Y., Yang, S., Zeng, M., Zhang, S., Liu, J., Han, Y., Liu, Y., Zhu, X., Wu, H., Liu, F., Sun, L., Xiao, L. Tags: PublishAheadOfPrint Source Type: research

Nicotine Enhances Mesangial Cell Proliferation and Fibronectin Production in High Glucose Milieu via Activation of Wnt/ β-catenin Pathway.
In this study, we examined the involvement of Wnt/β-catenin pathway in nicotine-mediated mesangial cell growth in high glucose milieu. Primary human renal mesangial cells were treated with nicotine in the presence of normal (5 mM) or high glucose (30 mM) followed by evaluation for cell growth. In the presence of normal glucose, nicotine increased both the total cell numbers and Ki-67 positive cell ratio, indicating that nicotine stimulated mesangial cell proliferation. Although high glucose itself also stimulated mesangial cell proliferation, nicotine further enhanced the mitogenic effect of high glucose. Similarly, nicot...
Source: Bioscience Reports - March 23, 2018 Category: Biomedical Science Authors: Lan X, Wen H, Aslam R, Marashi Shoshtari SS, Mishra A, Kumar V, Wang H, Wu G, Luo H, Malhotra A, Singhal PC Tags: Biosci Rep Source Type: research

HSPB8 Promotes the Fusion of Autophagosome and Lysosome during Autophagy in Diabetic Neurons.
Authors: Li XC, Hu QK, Chen L, Liu SY, Su S, Tao H, Zhang LN, Sun T, He LJ Abstract Although autophagy has been proposed to play an emerging role in diabetic neuropathy, autophagy and its possible role remains unclear. Moreover, only few studies about diabetes have explored the autophagy mediated by heat shock protein beta-8 (HSPB8) and Bcl-2 associated athanogene 3 (BAG3). In the present study, we examined the autophagy induced by high glucose levels in an in vivo rat model of diabetes induced by streptozotocin (STZ) and an in vitro model of retinal ganglion cell-5 (RGC5) cells under high glucose conditions. In th...
Source: International Journal of Medical Sciences - December 6, 2017 Category: Biomedical Science Tags: Int J Med Sci Source Type: research