High Glucose Provokes Microvesicles Generation from Glomerular Podocytes via NOX4/ROS Pathway.

High Glucose Provokes Microvesicles Generation from Glomerular Podocytes via NOX4/ROS Pathway. Biosci Rep. 2019 Oct 30;: Authors: Li M, Zhang T, Wu X, Chen Y, Sun L Abstract Microvesicles (MVs) were involved in the pathogenesis of many diseases, such as cardiovascular diseases and diabetes. Oxidative stress played a key role in the development and progression of diabetic nephropathy. Our aim of this study was to investigate whether high glucose could provoke microvesicles generation from podocytes and its potential mechanism. Mouse podocyte clone 5 (MPC-5) were stimulated by high glucose. The intracellular reactive oxygen species (ROS) of podocytes were measured by fluorescence microscopy with the probe of CM-H2DCFDA and MitoSOXTM. Antioxidants N-Acetyl-L-cysteine (NAC) and alpha lipoic acid (α-LA) were used to treat podocytes after high glucose stimulation. The rate of podocyte apoptosis was evaluated with Annexin V-FITC by flow cytometry. NOX4 expression was examined and siRNA were performed to explore the mechanism of MVs generation. The quantities of MVs from MPC-5 cells was significantly increased (P<0.05) by 4.6 times after 30 mM glucose stimulation, accompanied with double increased apoptosis. Cellular ROS generation was increased by high glucose at the peak of 48h stimulation. High glucose induced MVs was significantly decreased by 52.9% after pretreatment by antioxidant NAC. Nevertheless, Mitochondrial ROS in podoc...
Source: Bioscience Reports - Category: Biomedical Science Authors: Tags: Biosci Rep Source Type: research