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Condition: Reflex Sympathetic Dystrophy
Cancer: Neuroblastoma

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Total 18 results found since Jan 2013.

GSE161899 The dependency factor RRM2 at the nexus of a copy number driven regulatory network and a target for synthetic lethal interactions with replication stress checkpoint addiction in high-risk neuroblastoma siRNA_RRM2
In this study, we identified and functionally evaluated the ‘ribonucleotide reductase regulatory subunit 2’ (RRM2) as a top-ranked 2p putative co-driver and therapeutic target in high-risk neuroblastoma enforcing replicative stress resistance. RRM2 dependency was shown in vitro through knockdown in neuroblastoma cells, with transcriptome analysis reveali ng downregulation of pediatric tumor markers. Transgenic zebrafish lines expressing RRM2 in immature sympathoblasts were crossed with a dh-MYCN transgenic zebrafish line, with RRM2 overexpression dramatically increasing tumor penetrance from 20% to nearly 100%, acce...
Source: GEO: Gene Expression Omnibus - August 3, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Hypoxia-Inducible Factor-1 α (HIF-1α) Inhibition Impairs Retinoic Acid-Induced Differentiation in SH-SY5Y Neuroblastoma Cells, Leading to Reduced Neurite Length and Diminished Gene Expression Related to Cell Differentiation
Neurochem Res. 2021 Sep 23. doi: 10.1007/s11064-021-03454-3. Online ahead of print.ABSTRACTNeuroblastoma is the most common extracranial solid tumour in childhood, originated from cells of the neural crest during the development of the Sympathetic Nervous System. Retinoids are vitamin-A derived differentiating agents utilised to avoid disease resurgence in high-risk neuroblastoma treatment. Several studies indicate that hypoxia-a common feature of the tumoural environment-is a key player in cell differentiation and proliferation. Hypoxia leads to the accumulation of the hypoxia-inducible factor-1α (HIF-1α). This work aim...
Source: Neurochemical Research - September 24, 2021 Category: Neuroscience Authors: Pedro Ozorio Brum Guilherme Danielski Viola Carolina Saibro-Girardi Camila Tiefensee-Ribeiro Matheus Ozorio Brum Juciano Gasparotto Rachel Krolow Jos é Cláudio Fonseca Moreira Daniel Pens Gelain Source Type: research

Hypoxia-Inducible Factor-1alpha (HIF-1alpha) Inhibition Impairs Retinoic Acid-Induced Differentiation in SH-SY5Y Neuroblastoma Cells, Leading to Reduced Neurite Length and Diminished Gene Expression Related to Cell Differentiation
Neurochem Res. 2021 Sep 23. doi: 10.1007/s11064-021-03454-3. Online ahead of print.ABSTRACTNeuroblastoma is the most common extracranial solid tumour in childhood, originated from cells of the neural crest during the development of the Sympathetic Nervous System. Retinoids are vitamin-A derived differentiating agents utilised to avoid disease resurgence in high-risk neuroblastoma treatment. Several studies indicate that hypoxia-a common feature of the tumoural environment-is a key player in cell differentiation and proliferation. Hypoxia leads to the accumulation of the hypoxia-inducible factor-1α (HIF-1α). This work aim...
Source: Cell Research - September 24, 2021 Category: Cytology Authors: Pedro Ozorio Brum Guilherme Danielski Viola Carolina Saibro-Girardi Camila Tiefensee-Ribeiro Matheus Ozorio Brum Juciano Gasparotto Rachel Krolow Jos é Cláudio Fonseca Moreira Daniel Pens Gelain Source Type: research

NEAT1 Negatively Regulates Cell Proliferation and Migration of Neuroblastoma Cells by miR-183-5p/FOXP1 Via the ERK/AKT Pathway.
Abstract Neuroblastoma, a malignant tumor of the sympathetic nervous system, is an aggressive extracranial tumor in childhood. Long noncoding RNAs (lncRNAs) have been discovered to play a key role in the eukaryotic regulatory gene network and be involved in a wide variety of biological processes. We observed that the expression of lncRNA nuclear-enriched abundant transcript-1 (NEAT1) was significantly decreased in human neuroblastoma tissues and cell lines, compared with the normal. We observed cell proliferation, migration, and invasion with Cell Counting Kit-8 assay, colony formation assay, and Transwell assay t...
Source: Cell Transplantation - December 31, 2019 Category: Cytology Authors: Pan W, Wu A, Yu H, Yu Q, Zheng B, Yang W, Tian D, Gao Y, Li P Tags: Cell Transplant Source Type: research

Connecting Metainflammation and Neuroinflammation Through the PTN-MK-RPTP β/ζ Axis: Relevance in Therapeutic Development
Conclusion The expression of the components of the PTN-MK-RPTPβ/ζ axis in immune cells and in inflammatory diseases suggests important roles for this axis in inflammation. Pleiotrophin has been recently identified as a limiting factor of metainflammation, a chronic pathological state that contributes to neuroinflammation and neurodegeneration. Pleiotrophin also seems to potentiate acute neuroinflammation independently of the inflammatory stimulus while MK seems to play different -even opposite- roles in acute neuroinflammation depending on the stimulus. Which are the functions of MK and PTN in chronic neuroi...
Source: Frontiers in Pharmacology - April 11, 2019 Category: Drugs & Pharmacology Source Type: research

Cancers, Vol. 11, Pages 202: SYK Inhibition Potentiates the Effect of Chemotherapeutic Drugs on Neuroblastoma Cells in Vitro
In this study, we observed elevated SYK gene expression in neuroblastoma compared to neural crest and benign neurofibroma. While SYK protein was detected in the majority of examined neuroblastoma tissues it was less frequently observed in neuroblastoma cell lines. Depletion of SYK by siRNA and the use of small molecule SYK inhibitors significantly reduced the cell viability of neuroblastoma cell lines expressing SYK protein. Moreover, SYK inhibition decreased ERK1/2 and Akt phosphorylation. The SYK inhibitor BAY 613606 enhanced the effect of different chemotherapeutic drugs. Transient expression of a constitutive active SY...
Source: Cancers - February 10, 2019 Category: Cancer & Oncology Authors: Conny T ümmler Gianina Dumitriu Malin Wickstr öm Peter Coopman Andrey Valkov Per Kogner John Inge Johnsen Ugo Moens Baldur Sveinbj örnsson Tags: Article Source Type: research

Abstract PR09: MYCN and is a therapeutic target in neuroblastoma
Neuroblastoma is a childhood cancer of the sympathetic nervous system. Approximately 20% of patients present with an aggressive metastatic disease characterized by amplification of the MYCN locus. However, an additional 30% of patients suffer from an equally aggressive disease, yet there are no unified genetic markers available.To identify potential biomarkers of poor prognosis in neuroblastoma, we conducted unsupervised hierarchical clustering on 649 primary neuroblastoma tumors based on a 51 gene MYC core target signature. This demonstrated strong activation within the MYCN amplified cohort as expected, but surprisingly ...
Source: Molecular Cancer Research - October 18, 2015 Category: Cancer & Oncology Authors: Carter, D. R., Murray, J., Cheung, B. B., Kalla, H., Gamble, L., Tsang, J., Sutton, S., Koach, J., Syed, S., Gifford, A., Issaeva, N., Biktasova, A., Atmadibrata, B., Sun, Y., Sokolowski, N., Ling, D., Kim, P. Y., Webber, H., Clark, A., Ruhle, M., Liu, B. Tags: Targeting Myc-Driven Cancers: Oral Presentations - Proffered Abstracts Source Type: research

Abstract 3266: Inhibition of Rho-associated kinase 2 suppresses MYCN expression and induces differentiation of neuroblastoma
Conclusions: Our results show the significance of the non-canonical/PCP signaling cascade in neuroblastoma development and progression. Furthermore, ROCK inhibition suppress the growth of neuroblastoma in preclinical models and provide a novel option for improved treatment of high-risk neuroblastoma.Citation Format: Cecilia Dyberg, David Forsberg, Susanne Fransson, Jessika Lännerholm Palm, Bjorn Helge Haug, Baldur Sveinbjornsson, Tommy Martinsson, Per Kogner, John Inge Johnsen, Malin Wickstrom. Inhibition of Rho-associated kinase 2 suppresses MYCN expression and induces differentiation of neuroblastoma. [abstract]. In: Pr...
Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Dyberg, C., Forsberg, D., Fransson, S., Lannerholm Palm, J., Haug, B. H., Sveinbȷornsson, B., Martinsson, T., Kogner, P., Johnsen, J. I., Wickstrom, M. Tags: Tumor Biology Source Type: research

Abstract 475: Identification of SHANK2 as a tumor suppressor disrupted by recurrent somatic structural variation (SV) in neuroblastoma
Background. Neuroblastoma is a malignancy of the developing sympathetic nervous system which exacts significant morbidity and mortality in children. Large sequencing efforts have revealed a relative paucity of somatic point mutations in this and other childhood cancers. Structural variations (SVs) including translocations, inversions, deletions, duplications, and other complex events can occur in germline DNA or be acquired somatically in tumors. Recurrent somatic SVs affecting known genes are likely to be functional and may elucidate novel tumor suppressors or oncogenes.Methods. We are performing whole-genome sequencing (...
Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Conkrite, K., Ferraro, N., McDaniel, L., Oldridge, D. A., Attiyeh, E., Asgharzadeh, S., Diamond, M., Guidry Auvil, J., Davidsen, T., Smith, M., London, W. B., Seeger, R., Khan, J., Gerhard, D. S., Maris, J. M., Diskin, S. J. Tags: Tumor Biology Source Type: research

Abstract 191: The role of microRNAs in the epigenetic silencing of CHD5, a tumor suppressor in neuroblastoma
Background: Neuroblastoma (NB) is a tumor of the sympathetic nervous system that is the most common extracranial solid tumor of childhood. NBs show remarkable clinical heterogeneity, and we, along with others, have identified different patterns of genomic change that underlie these diverse clinical behaviors. We first identified 1p deletion as a characteristic change in advanced stage NBs. CHD5, a tumor suppressor gene, was first identified because of its location on 1p36. However, mutation of the remaining allele of CHD5 is rare in these tumors. Therefore, it is likely that epigenetic mechanisms play important roles in CH...
Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Naraparaju, K., Kolla, V., Zhuang, T., Higashi, M., Blobel, G. A., Brodeur, G. M. Tags: Molecular and Cellular Biology Source Type: research

Transcriptional co-activator TAZ sustains proliferation and tumorigenicity of neuroblastoma by targeting CTGF and PDGF-β.
Authors: Wang M, Liu Y, Zou J, Yang R, Xuan F, Wang Y, Gao N, Cui H Abstract Neuroblastoma is a common childhood malignant tumor originated from the neural crest-derived sympathetic nervous system. A crucial event in the pathogenesis of neuroblastoma is to promote proliferation of neuroblasts, which is closely related to poor survival. However, mechanisms for regulation of cell proliferation and tumorigenicity in neuroblastoma are not well understood. Here, we report that overexpression of TAZ in neuroblastoma BE(2)-C cells causes increases in cell proliferation, self renewal and colony formation, which was restore...
Source: Oncotarget - May 6, 2015 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Abstract 3105: Neuropeptide Y Y5 receptor in neuroblastoma chemoresistance
Neuroblastoma (NB) is a pediatric tumor with heterogeneous phenotypes. While low stage tumors carry favorable prognosis, over 50% of high risk NB relapses after treatment with fatal outcome. Thus, developing therapies targeting this refractory form of NB remains an unsolved clinical problem. Neuropeptide Y (NPY) is a sympathetic neurotransmitter released from NB cells. High systemic levels of NPY are associated with poor clinical outcome of the disease, which is in agreement with its proliferative effect in NB cells and angiogenic properties. While all of the above functions of NPY are mediated mainly by its Y2 receptor (Y...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Trinh, E., Czarnecka, M., Hong, S.-H., Lu, C., Martin, S., Galli, S., Izycka-Swieszewska, E., Kuan-Celarier, A., Christian, D., Horton, M., Tilan, J. U., Kitlinska, J. B. Tags: Tumor Biology Source Type: research

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