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Expression and Purification of FGFR1-Fc Fusion Protein and Its Effects on Human Lung Squamous Carcinoma
Appl Biochem Biotechnol. 2023 May 9. doi: 10.1007/s12010-023-04542-6. Online ahead of print.ABSTRACTMolecular-targeted therapies for lung squamous cell carcinoma (LSCC) are limited mainly because targetable oncogenic aberrations are absent in LSCC. Recent genomic analyses have revealed that the fibroblast growth factor (FGF) signaling pathway plays a fundamental role in LSCC progression via cancer cell proliferation and angiogenesis. In the present study, we designed, expressed, and purified a fibroblast growth factor receptor fragment (FGFR1-Fc) fusion protein using NS/0 cells. In FGF2-FGFR1 overexpressed NCI-H1703 cells,...
Source: Applied Biochemistry and Biotechnology - May 9, 2023 Category: Biochemistry Authors: Lulu Zheng Huan Liu Lingfeng Chen Xinyi You Fangyi Lv Haibing Fan Qi Hui Baohua Liu Xiaojie Wang Source Type: research

ANXA2 as a novel substrate of FBXW7 promoting esophageal squamous cell carcinoma via ERK phosphorylation
In conclusion, this study provided evidence that FBXW7 loss of function could promote esophageal cancer through ANXA2 overexpression, and this novel regulation pathway may be used as an efficient target for ESCC treatment.PMID:36758484 | DOI:10.1016/j.bbrc.2023.01.082
Source: Biochemical and Biophysical Research communications - February 9, 2023 Category: Biochemistry Authors: Zongfei Li Yunzhi Pan Jiayi Yao Yingyin Gao Yulan Qian Minxue Zheng Sai Ma Source Type: research

Evaluating the effect of siRNA on sox2ot expression in the Human Neuron-Committed Teratocarcinoma NT2 Cellline.
Authors: Sadeghi Z, Dodange F, Maleki P, Zarei M, Taheri M, Raheb J Abstract Non-coding RNA elongated (lncRNAs) have recently attracted as molecules that regulate gene expression of the pluripotent properties (pluripotency) of stem cells. Recently our colleagues examined the role of one of these RNAs called SOX2OT in esophageal squamous cell carcinoma, and found a concomitant increase in its expression with some regulatory genes of cell proliferation. In the present study, using the design of suitable primers from SOX2OT gene, we investigated the effect of siRNA on expression of SOX2OT. PMID: 32716352 [PubMed -...
Source: Human Antibodies - July 29, 2020 Category: Biochemistry Tags: Hum Antibodies Source Type: research

Downregulation of nicotinamide N-methyltransferase inhibits migration and epithelial-mesenchymal transition of esophageal squamous cell carcinoma via Wnt/ β-catenin pathway.
Downregulation of nicotinamide N-methyltransferase inhibits migration and epithelial-mesenchymal transition of esophageal squamous cell carcinoma via Wnt/β-catenin pathway. Mol Cell Biochem. 2019 Jul 05;: Authors: Cui Y, Zhang L, Wang W, Ma S, Liu H, Zang X, Zhang Y, Guan F Abstract Nicotinamide N-methyltransferase (NNMT) is an important methyltransferase involved in the biotransformation of many drugs and exogenous compounds. Abnormal expression of NNMT protein is closely associated with the onset and progression of many malignancies, but little is known about its role in esophageal squamous cell ca...
Source: Molecular and Cellular Biochemistry - July 4, 2019 Category: Biochemistry Authors: Cui Y, Zhang L, Wang W, Ma S, Liu H, Zang X, Zhang Y, Guan F Tags: Mol Cell Biochem Source Type: research

Voltage-gated sodium channel Nav1.5 promotes proliferation, migration and invasion of oral squamous cell carcinoma.
In this study, we found that Nav1.5 was highly expressed in OSCC cell lines compared with normal oral keratinocyte HOK cell line by using western blot analysis. CCK-8 assay results revealed that downregulation of Nav1.5 expression by its specific siRNA reduced proliferation of OSCC HSC-3 cells. Moreover, transwell assay results showed Nav1.5 knockdown significantly inhibited migration and invasion of HSC-3 cells. Meanwhile, qRT-PCR and western blot analysis results showed that epidermal growth factor (EGF) induced Nav1.5 expression in a time- and dose-dependent manner. In addition, EGF promoted proliferation, migration and...
Source: Acta Biochimica et Biophysica Sinica - May 28, 2019 Category: Biochemistry Authors: Zhang J, Mao W, Dai Y, Qian C, Dong Y, Chen Z, Meng L, Jiang Z, Huang T, Hu J, Luo P, Korner H, Jiang Y, Ying S Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Delivery of MTH1 inhibitor (TH287) and MDR1 siRNA via hyaluronic acid-based mesoporous silica nanoparticles for oral cancers treatment
Publication date: 1 January 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 173Author(s): Xiao-Lei Shi, Yuan Li, Lu-Ming Zhao, Lin-Wang Su, Gang DingAbstractOral cancer accounts for 95% of all maxillofacial malignant neoplasm. Presently, surgery and radiation (with and without chemotherapy) are the major treatments for oral cancer; however it met with limited therapeutic outcome. Overcoming the multidrug resistance and finding new therapeutic agents for oral cancer treatment are some of the serious challenges. Small molecule, TH287 potently and selectively inhibits the MTH1 protein in cells and could act as a ne...
Source: Colloids and Surfaces B: Biointerfaces - October 23, 2018 Category: Biochemistry Source Type: research

RHCG suppresses cervical cancer progression through inhibiting migration and inducing apoptosis regulated by TGF- β1.
RHCG suppresses cervical cancer progression through inhibiting migration and inducing apoptosis regulated by TGF-β1. Biochem Biophys Res Commun. 2018 May 28;: Authors: Wang DG, Li TM, Liu X Abstract Cervical cancer is the second commonest cancer among women in the worldwide, and the majority cause of death in various countries, highlighting the importance of investigating new therapeutic targets. Rh family, C glycoprotein (RHCG) belongs to the Rhesus (Rh) family and was first identified as Rh blood group antigens. It has been confirmed to function in cancer progression, including prostate cancer and ...
Source: Biochemical and Biophysical Research communications - May 28, 2018 Category: Biochemistry Authors: Wang DG, Li TM, Liu X Tags: Biochem Biophys Res Commun Source Type: research

IGF2BP1 over-expression in skin squamous cell carcinoma cells is essential for cell growth.
Abstract The present study examined expression and potential functions of insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) in human skin squamous cell carcinoma (SCC). We show that IGF2BP1 mRNA and protein expression levels were upregulated in established (A431 line) and primary human skin SCC cells. Its expression was also increased in human skin SCC tissues, as compared to the normal skin tissues. In skin SCC cells, IGF2BP1 silencing or CRISPR/Cas9 knockout decreased levels of IGF2BP1-stablized mRNAs, including IGF2, CD44, Gli1 and Myc. Furthermore, skin SCC cell survival and proliferation were in...
Source: Biochemical and Biophysical Research communications - May 10, 2018 Category: Biochemistry Authors: Liu Z, Wu G, Lin C, Guo H, Xu J, Zhao T Tags: Biochem Biophys Res Commun Source Type: research

Inhibitory effect of microRNA-154 targeting WHSC1 on cell proliferation of human skin squamous cell carcinoma through mediating the P53 signaling pathway.
CONCLUSION: These results demonstrate that up-regulation of miR-154 inhibits proliferation and induces apoptosis of human skin SCC cells by down-regulating WHSC1 and blocking the P53 signaling pathway. PMID: 29727714 [PubMed - as supplied by publisher]
Source: The International Journal of Biochemistry and Cell Biology - May 1, 2018 Category: Biochemistry Authors: Chen HQ, Gao D Tags: Int J Biochem Cell Biol Source Type: research

Genome-wide identification of chromatin-enriched RNA reveals that unspliced dentin matrix protein-1 mRNA regulates cell proliferation in squamous cell carcinoma.
Abstract Chromatin-enriched noncoding RNAs (ncRNAs) have emerged as key molecules in epigenetic processes by interacting with chromatin-associated proteins. Recently, protein-coding mRNA genes have been reported to be chromatin-tethered, similar with ncRNA. However, very little is known about whether chromatin-enriched mRNA is involved in the chromatin modification process. Here, we comprehensively examined chromatin-enriched RNA in squamous cell carcinoma (SQCC) cells by RNA subcellular localization analysis, which was a combination of RNA fractionation and RNA-seq. We identified 11 mRNAs as highly chromatin-enri...
Source: Biochemical and Biophysical Research communications - December 23, 2017 Category: Biochemistry Authors: Suzuki S, Hoshino H, Yoshida K, Nakanishi J, Tsuchiya-Hirata S, Kobuke S, Haruyama N, Nishimura F, Shiba H Tags: Biochem Biophys Res Commun Source Type: research

Comprehensive bioinformation analysis of the miRNA of PLCE1 knockdown in esophageal squamous cell carcinoma.
In this study, PLCE1 siRNA and vector-only plasmid were stably transfected into Eca109 and EC9706 cells and then subjected to miRNA array analysis, and quantitative real-time PCR was applied to validate miRNA array data. Then bioinformatic analyses, such as GO and pathway software, were conducted to obtain data on these differentially expressed miRNAs-targeted genes (DEGs) and clarify their function and pathway. The results showed that 36 miRNAs were found to be differentially expressed in PLCE1 siRNA-transfected cells compared with the control cells. In particular, 28 miRNAs were upregulated while 8 miRNAs were downregula...
Source: Molecular and Cellular Biochemistry - December 13, 2017 Category: Biochemistry Authors: Cui X, Wang K, Yang X, Peng H, Chen X, Xin H, Tian Y, Chen Y, Li F Tags: Mol Cell Biochem Source Type: research

Effect of TET1 regulating MGMT on chemotherapy resistance of oral squamous cell carcinoma stem cells
This article is protected by copyright. All rights reserved
Source: Journal of Cellular Biochemistry - June 23, 2017 Category: Biochemistry Authors: Wei Wang, Xin Li, Fan Wang, Xiang ‐Yu Sun Tags: Article Source Type: research

Minichromosome maintenance complex is required for checkpoint kinase 2 chromatin loading and its phosphorylation to DNA damage response in SCC-4 cells.
Abstract Checkpoint kinase 2 (Chk2) is a significant mediator of diverse responses to DNA damage. The present study was aimed to identify possible interactive proteins of Chk2 and try to clarify the underlying mechanism regarding Chk2 chromatin loading and its phosphorylation to DNA damage response in oral squamous cell carcinoma (OSCC). Differently tagged Chk2 and minichromosome maintenance (MCM) complex (MCM2, MCM3, MCM5, and MCM6) were overexpressed into SCC-4 cells. After 48 h of transfection cell fractionation was performed to localize proteins. In addition, immunoreactive species were detected by immunopreci...
Source: Protein and Peptide Letters - December 11, 2016 Category: Biochemistry Authors: Gong Z, Feng Y, Yu B, Zhang R, Zhang P, Cao M, Liu H, Yang T, Luo R Tags: Protein Pept Lett Source Type: research

Transforming growth factor-{beta}1 induces invasion ability of HSC-4 human oral squamous cell carcinoma cells through the Slug/Wnt-5b/MMP-10 signalling axis
Molecular mechanism underlying the invasion of oral cancer cells remains to be clarified. We previously demonstrated that transforming growth factor-β1 (TGF-β1) induces the expression of mesenchymal markers in human oral squamous cell carcinoma HSC-4 cells. Intriguingly, the expression of the epithelial–mesenchymal transition-related transcription factor Slug was also significantly upregulated upon TGF-β1 stimulation. However, the mechanism by which Slug transduces the TGF-β1-induced signal to enhance the invasiveness of HSC-4 cells is poorly understood. Proteomic analysis revealed that the expres...
Source: Journal of Biochemistry - May 30, 2016 Category: Biochemistry Authors: Hino, M., Kamo, M., Saito, D., Kyakumoto, S., Shibata, T., Mizuki, H., Ishisaki, A. Tags: Regular Papers Source Type: research

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