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Specialty: Biochemistry
Cancer: Osteosarcoma

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Total 24 results found since Jan 2013.

TRIM4 Expression Related to Malignant Progression and Cisplatin Resistance in Osteosarcoma
This study explored the role of TRIM4 in OS chemotherapy sensitivity and malignant progression. The expression of TRIM4 in OS tissues and cells was examined by RT-qPCR, immunohistochemical staining, and western blot. Specific siRNA was transfected into U2-OS and SAOS2 cells to target TRIM4. Cell biological behavior was examined by CCK-8, Transwell, and flow cytometry experiments. Cisplatin-resistant SAOS2 (SAOS2-Cis-R) cells were established, and the effect of TRIM4 expression on the cisplatin response of SAOS2 cells was tested. Knockdown of TRIM4 significantly inhibited the proliferation, migration, and invasion of U2-OS ...
Source: Applied Biochemistry and Biotechnology - April 28, 2023 Category: Biochemistry Authors: Yan Li Jie Gao Dong Wang Zijin Liu Huawu Zhang Source Type: research

Targeting GRP78 enhances the sensitivity of HOS osteosarcoma cells to pyropheophorbide- α methyl ester-mediated photodynamic therapy via the Wnt/β-catenin signaling pathway
Acta Biochim Biophys Sin (Shanghai). 2021 Sep 8:gmab115. doi: 10.1093/abbs/gmab115. Online ahead of print.ABSTRACTPhotodynamic therapy (PDT), which is a new method for treating tumors, has been used in the treatment of cancer. In-depth research has shown that PDT cannot completely kill tumor cells, indicating that tumor cells are resistant to PDT. Glucose regulatory protein 78 (GRP78), which is a key regulator of endoplasmic reticulum stress, has been confirmed to be related to tumor resistance and recurrence, but there are relatively few studies on the further mechanism of GRP78 in PDT. Our experiment aimed to observe the...
Source: Acta Biochimica et Biophysica Sinica - September 8, 2021 Category: Biochemistry Authors: Qiang Zuo Yunsheng Ou Shenxi Zhong Haoyang Yu Fangbiao Zhan Muzi Zhang Source Type: research

miR-487a performs oncogenic functions in osteosarcoma by targeting BTG2 mRNA.
In this study, we elucidated the involvement of miR-487a in OS and the underlying molecular mechanisms. We found that miR-487a was upregulated in OS clinical samples and cell lines. Knockdown of miR-487a suppressed OS cell growth and invasion and induced apoptosis; however, overexpression of miR-487a promoted OS cell growth and invasion. Accordingly, downregulation of miR-487a significantly suppressed tumor growth of OS xenografts in vivo. Furthermore, B-cell translocation gene 2 (BTG2) mRNA was found to be a novel target of miR-487a. Knockdown of BTG2 using small interfering RNA (siRNA) recapitulated the oncogenic effects...
Source: Acta Biochimica et Biophysica Sinica - May 12, 2020 Category: Biochemistry Authors: Gu Z, Wu S, Xu G, Wu W, Mao B, Zhao S Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Down-regulation of RAC2 by small interfering RNA restrains the progression of osteosarcoma by suppressing the Wnt signaling pathway.
Abstract Osteosarcoma (OS) is the most common primary malignancy of bone and is characterized by a high malignant and metastatic potential. Microarray-based differentially expressed gene screening determined RAC2 as the candidate gene related to OS. Highly expressed RAC2 and activated Wnt signaling pathway were determined in OS tissues using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot analysis. The OS cells were transfected with siRNA-RAC2 or treated with BIO (activator of Wnt pathway), whereby the effects of siRNA-RAC2 on cell proliferation, invasion, cycle and apoptosi...
Source: International Journal of Biological Macromolecules - July 2, 2019 Category: Biochemistry Authors: Xia P, Gao X, Shao L, Chen Q, Li F, Wu C, Zhang W, Sun Y Tags: Int J Biol Macromol Source Type: research

Upregulated TTYH2 expression is critical for the invasion and migration of U2OS human osteosarcoma cell lines.
Abstract Ion channels have recently emerged as stable biomarkers and anticancer targets particularly when the applications of the currently available therapeutic regimens are limited, as in case of osteosarcoma, a malignant bone tumor. Here, we evaluated the expression of TTYH2, a presumably calcium-activated chloride channel, in a human osteosarcoma cell line U2OS. We used small-interfering RNA (siRNA)-mediated gene silencing to demonstrate the downregulation in the expression of TTYH2 that resulted in the decrease in the invasion and migration, but not proliferation, of U2OS cells. The expression levels of Slug ...
Source: Biochemical and Biophysical Research communications - June 19, 2019 Category: Biochemistry Authors: Moon DK, Bae YJ, Jeong GR, Cho CH, Hwang SC Tags: Biochem Biophys Res Commun Source Type: research

Proliferation inhibition and apoptosis promotion by dual silencing of VEGF and Survivin in human osteosarcoma.
Abstract Simultaneous silencing of multiple upregulated genes is an attractive and viable treatment strategy for many incurable diseases including cancer. Herein we used a dual gene-targeted siRNA conjugate composed of VEGF and Survivin siRNA sequences in the same backbone to inhibit proliferation and angiogenesis in two human osteosarcoma cell lines. We synthesized siRNA sequences targeting the VEGF and Survivin genes individually (VEGF siRNA and Survivin siRNA) or simultaneously (one-chain-double-target siRNA: dual siRNA). VEGF and Survivin mRNA and protein expression levels in human osteosarcoma MG-63 and Saos-...
Source: Acta Biochimica et Biophysica Sinica - December 19, 2018 Category: Biochemistry Authors: Gu J, Ji Z, Li D, Dong Q Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Carboxypeptidase E- ΔN promotes migration, invasion and epithelial-mesenchymal transition of human osteosarcoma cell lines through the Wnt/β-catenin pathway.
In this study, we investigated the effect of CPE-ΔN on cell migration, invasion and epithelial-mesenchymal transition (EMT) of OS cells, and illustrated the molecular mechanisms. We first constructed CPE-ΔN overexpressing human OS cell lines (143B and U2OS cells), and found that ectopic CPE-ΔN expression in OS cells enhanced cellular migratory and invasiveness abilities, and promoted EMT process. Further, overexpression of CPE-ΔN increased c-myc and nuclear β-catenin levels in OS cells, which suggested CPE-ΔN promoted the activation of Wnt/β-catenin pathway in OS cells. Treatment with β-catenin small interfering RN...
Source: Biochemistry and Cell Biology - December 3, 2018 Category: Biochemistry Authors: Fan S, Gao X, Chen P, Li X Tags: Biochem Cell Biol Source Type: research

Long non-coding RNA THOR promotes human osteosarcoma cell growth in vitro and in vivo.
Abstract Long non-coding RNA (LncRNA) dysregulation is associated with human osteosarcoma (OS) cell progression. Recent studies have characterized a novel but ultra-conserved LncRNA THOR ("Lnc-THOR") as a cancer-specific LncRNA, mediating cell growth. In the current study, we show that Lnc-THOR is expressed in established and primary human OS cells. It is also detected in human OS tissues, but not in the surrounding normal bone tissues. siRNA-induced knockdown or CRSIPR/Cas9-mediated knockout Lnc-THOR significantly inhibited human OS cell survival and proliferation. Insulin-like growth factor 2 mRNA-binding protei...
Source: Biochemical and Biophysical Research communications - April 4, 2018 Category: Biochemistry Authors: Chen W, Chen M, Xu Y, Chen X, Zhou P, Zhao X, Pang F, Liang W Tags: Biochem Biophys Res Commun Source Type: research

Expression and therapeutic implications of cyclin-dependent kinase 4 (CDK4) in osteosarcoma.
Abstract Overexpression and/or hyperactivation of cyclin-dependent kinase 4 (CDK4) has been found in many types of human cancers, and a CDK4 specific inhibitor, palbociclib, has been recently approved by the FDA for the treatment of breast cancer. However, the expression and the therapeutic potential of CDK4 in osteosarcoma remain unclear. In the present study, CDK4 was found to be highly expressed in human osteosarcoma tissues and cell lines as compared with normal human osteoblasts. Elevated CDK4 expression correlated with metastasis potential and poor prognosis in osteosarcoma patients as determined by immunohi...
Source: Biochimica et Biophysica Acta - February 13, 2018 Category: Biochemistry Authors: Zhou Y, Shen JK, Yu Z, Hornicek FJ, Kan Q, Duan Z Tags: Biochim Biophys Acta Source Type: research

SENP1/HIF ‐1α feedback loop modulates hypoxia‐induced cell proliferation, invasion, and EMT in human osteosarcoma cells
Hypoxia is an element intrinsic to most solid‐tumor microenvironments, including that of osteosarcoma (OS), and is associated with resistance to therapy, poor survival, and a malignant phenotype. The purpose of the present study was to investigate the role and underlying mechanism of SUMO‐specific protease 1 (SENP1)/hypoxia‐inducible factor‐1α (HIF‐1α) feedback loop in hypoxic microenvironment of OS. We observed that the expression of SENP1 was remarkably upregulated in OS cells. Additionally, there was a concomitant high expression of HIF‐1α and SENP1 in MG‐63 cells under a hypoxic microenvironment. Inter...
Source: Journal of Cellular Biochemistry - September 27, 2017 Category: Biochemistry Authors: Xiaowei Wang, Xiaoju Liang, Huan Liang, Bing Wang Tags: RESEARCH ARTICLE Source Type: research

SENP1/HIF ‐1α feedback loop modulates hypoxia‐induced cell proliferation, invasion and EMT in human osteosarcoma cells
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Source: Journal of Cellular Biochemistry - August 10, 2017 Category: Biochemistry Authors: Xiaowei Wang, Xiaoju Liang, Huan Liang, Bing Wang Tags: Article Source Type: research

Pneumolysin-induced autophagy contributes to inhibition of osteoblast differentiation through downregulation of Sp1 in human osteosarcoma cells
Conclusion: The results suggest that PLY inhibits osteoblast differentiation by downregulation of Sp1 accompanied by induction of autophagy through ROS-mediated regulation of the AMPK/mTOR pathway. General significance: This study proposes a molecular mechanism for inhibition of osteoblast differentiation in response to PLY.
Source: Biochimica et Biophysica Acta (BBA) General Subjects - July 19, 2017 Category: Biochemistry Source Type: research

Long Noncoding RNA GAS5 Suppresses Cell Growth and Epithelial –Mesenchymal Transition in Osteosarcoma by Regulating the miR‐221/ARHI Pathway
This study explored the potential functions and mechanisms of GAS5 in the tumorigenesis of osteosarcoma. Here, the expression of GAS5, miR‐221 and aplasia Ras homologue member I (ARHI) was determined in osteosarcoma tissues and cells by Real‐time PCR (RT‐qPCR). The underlying mechanism of GAS5 in osteosarcoma growth was analyzed via MTT, Transwell, RT‐qPCR, Western blot, dual‐luciferase reporter assay, RNA immunoprecipitation, and xenograft models after GAS5 overexpression. GAS5 and ARHI levels were significantly reduced, while miR‐221 increased, both in osteosarcoma tissues and cells. Overexpression of GAS5 su...
Source: Journal of Cellular Biochemistry - June 13, 2017 Category: Biochemistry Authors: Kaishan Ye, Shuanke Wang, Haihong Zhang, Hua Han, Bing Ma, Wei Nan Tags: Article Source Type: research