Carboxypeptidase E- ΔN promotes migration, invasion and epithelial-mesenchymal transition of human osteosarcoma cell lines through the Wnt/β-catenin pathway.

In this study, we investigated the effect of CPE-ΔN on cell migration, invasion and epithelial-mesenchymal transition (EMT) of OS cells, and illustrated the molecular mechanisms. We first constructed CPE-ΔN overexpressing human OS cell lines (143B and U2OS cells), and found that ectopic CPE-ΔN expression in OS cells enhanced cellular migratory and invasiveness abilities, and promoted EMT process. Further, overexpression of CPE-ΔN increased c-myc and nuclear β-catenin levels in OS cells, which suggested CPE-ΔN promoted the activation of Wnt/β-catenin pathway in OS cells. Treatment with β-catenin small interfering RNA (siRNA) inhibited the migratory and invasive abilities of CPE-ΔN-overexpressing cells, and reversed E-cadherin expression. Together, these results suggest that CPE-ΔN promotes migration, invasion and EMT of OS cells via the Wnt/β-catenin signaling. PMID: 30508384 [PubMed - as supplied by publisher]
Source: Biochemistry and Cell Biology - Category: Biochemistry Authors: Tags: Biochem Cell Biol Source Type: research