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Specialty: Allergy & Immunology

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Total 72221 results found since Jan 2013.

Exosomes from iPSCs Delivering siRNA Attenuate Intracellular Adhesion Molecule-1 Expression and Neutrophils Adhesion in Pulmonary Microvascular Endothelial Cells
In this study, we reprogrammed urine exfoliated renal epithelial cells into human induced pluripotent stem cells (huiPSCs) and purified the exosomes (Exo) from huiPSCs as RNAi delivery system. Through choosing the episomal system to deliver transcription factors, we obtained a non-integrating huiPSCs. Experiments in bothvitro andvivo demonstrated that these huiPSCs possess the pluripotent properties. The exosomes of huiPSCs isolated by differential centrifugation were visualized by transmission electron microscopy (TEM) showing a typical exosomal appearance with an average diameter of 122  nm. Immunoblotting confirmed the...
Source: Inflammation - December 20, 2016 Category: Allergy & Immunology Source Type: research

A review of PD-1/PD-L1 siRNA delivery systems in immune T cells and cancer cells
CONCLUSION: The findings of this study indicated that several strategies for siRNA transfection to immune and cancer cells have been evaluated in recent decades, some of which effectively transfect siRNA to target cells, and defined PD-1 siRNA as a promising strategy for cancer treatment.PMID:35987146 | DOI:10.1016/j.intimp.2022.109022
Source: International Immunopharmacology - August 20, 2022 Category: Allergy & Immunology Authors: Mehdi Barati Farshad Mirzavi Mahdi Atabaki Bahram Bibak Mojgan Mohammadi Mahmoud Reza Jaafari Source Type: research

Naringenin Produces Neuroprotection Against LPS-Induced Dopamine Neurotoxicity via the Inhibition of Microglial NLRP3 Inflammasome Activation
Conclusions: This study demonstrated that NAR targeted microglial NLRP3 inflammasome to protect DA neurons against LPS-induced neurotoxicity. These findings suggest NAR might hold a promising therapeutic potential for PD. Background Parkinson's disease (PD) is the second most prevalent central nervous system (CNS) degenerative disease. It is characterized by slow and progressive loss of dopamine (DA) neurons in the midbrain substantia nigra (SN) with the accumulation of α-synuclein in Lewy bodies and neuritis (1). Although the etiology of PD remains unclear, amounts of studies have suggested that ne...
Source: Frontiers in Immunology - April 30, 2019 Category: Allergy & Immunology Source Type: research

siRNA containing a unique 5-nucleotide motif acts as a quencher of IFI16-mediated innate immune response
Publication date: October 2019Source: Molecular Immunology, Volume 114Author(s): Hongyan Sui, Jun Yang, Xiaojun Hu, Qian Chen, Tomozumi ImamichiAbstractWe previously reported that some small interfering RNA (siRNA) enhances DNA or DNA virus mediated-interferon (IFN)-λ1(a type III IFN) induction through the crosstalk between retinoic acid-inducible gene I (RIG-I) and interferon gamma-inducible protein 16 (IFI16) signalling pathway. Here we provide further evidence of a new role for siRNA. siRNA containing a 5-nucleotide (nt) motif sequence suppresses DNA-mediated not only type III IFNs, but also type I IFNs and inflammator...
Source: Molecular Immunology - August 21, 2019 Category: Allergy & Immunology Source Type: research

Adenovirus-Mediated Small Interfering RNA Targeting TAK1 Ameliorates Joint Inflammation with Collagen-Induced Arthritis in Mice
AbstractTransforming growth factor β-activated kinase-1 (TAK1) is a key upstream kinase in cell signaling during inflammation, which regulates the expression of inflammatory mediators. Small interfering RNA (siRNA) against TAK1 offers promise as a potential therapeutic strategy in immune-mediated inflammatory disorder including rheu matoid arthritis. Here, we are to evaluate the therapeutic effects of intra-articular administration of adenoviral-mediated siRNA against TAK1 (ad-siRNA-TAK1) on collagen-induced arthritis (CIA) in mice. Ad-siRNA-TAK1 was constructed. The murine RAW 264.7 macrophages were infected with ad-siRN...
Source: Inflammation - February 20, 2017 Category: Allergy & Immunology Source Type: research

The siRNA cocktail targeting IL-10 receptor and TGF-β receptor on dendritic cells potentiates tumor antigen-specific CD8(+) T cell immunity.
This article is protected by copyright. All rights reserved. PMID: 25753156 [PubMed - as supplied by publisher]
Source: Clinical and Developmental Immunology - March 8, 2015 Category: Allergy & Immunology Authors: Ahn YH, Hong SO, Kim JH, Noh KH, Song KH, Lee YH, Jeon JH, Kim DW, Seo JH, Kim TW Tags: Clin Exp Immunol Source Type: research

Chitosan nanoparticles as a dual drug/siRNA delivery system for treatment of colorectal cancer.
In conclusion, ChNPs encapsulating DOX and snail siRNA can be considered as an effective anti-cancer drugs delivery system for the treatment of colorectal cancer. PMID: 27916629 [PubMed - as supplied by publisher]
Source: Immunology Letters - November 30, 2016 Category: Allergy & Immunology Authors: Sadreddini S, Safaralizadeh R, Baradaran B, Aghebati-Maleki L, Hosseinpour-Feizi MA, Shanehbandi D, Jadidi-Niaragh F, Sadreddini S, Kafil HS, Younesi V, Yousefi M Tags: Immunol Lett Source Type: research

Activation of Cell Membrane-localized Toll-like Receptor 3 by siRNA.
Abstract Small interfering RNA molecules (siRNA) are short dsRNAs that are used for different therapeutic applications. On the other hand, dsRNAs can bind to and activate cell RNA sensors and consequently trigger inflammatory response. Here we show that siRNA activates primary human endothelial cells and human lymphatic endothelial cells and that this response is inhibited by antibodies against TLR3. In contrast, the activation of human lymphatic endothelial cells by poly(I:C) was inhibited by bafilomycin but not by anti-TLR3 antibodies. Bafilomycin also inhibited poly(I:C) but not siRNA cell stimulation in TLR3-t...
Source: Immunology Letters - April 6, 2017 Category: Allergy & Immunology Authors: Pirher N, Pohar J, Manček-Keber M, Benčina M, Jerala R Tags: Immunol Lett Source Type: research

The siRNA cocktail targeting IL‐10 receptor and TGF‐β receptor on dendritic cells potentiates tumor antigen‐specific CD8+ T cell immunity
This article is protected by copyright. All rights reserved.
Source: Clinical and Experimental Immunology - March 8, 2015 Category: Allergy & Immunology Authors: Ye‐Hyeon Ahn, Soon‐Oh Hong, Jin Hee Kim, Kyung Hee Noh, Kwon‐Ho Song, Young‐Ho Lee, Ju‐Hong Jeon, Dong‐Wan Kim, Jae Hong Seo, Tae Woo Kim Tags: Original Article Source Type: research

The siRNA cocktail targeting interleukin 10 receptor and transforming growth factor‐β receptor on dendritic cells potentiates tumour antigen‐specific CD8+ T cell immunity
In this study, we used small interfering RNA (siRNA) to silence the expression of endogenous molecules in DCs, which can sense immunosuppressive factors. Among the siRNAs targeting various immunosuppressive molecules, we observed that DCs transfected with siRNA targeting IL‐10 receptor alpha (siIL‐10RA) initiated the strongest antigen‐specific CD8+ T cell immune responses. The potency of siIL‐10RA was enhanced further by combining it with siRNA targeting TGF‐β receptor (siTGF‐βR), which was the next best option during the screening of this study, or the previously selected immunoadjuvant siRNA targeting phosp...
Source: Clinical and Experimental Immunology - May 17, 2015 Category: Allergy & Immunology Authors: Y.‐H. Ahn, S.‐O. Hong, J. H. Kim, K. H. Noh, K.‐H. Song, Y.‐H. Lee, J.‐H. Jeon, D.‐W. Kim, J. H. Seo, T. W. Kim Tags: Original Article Source Type: research

Complement C2 siRNA mediated therapy of myasthenia gravis in mice.
In this study, we show that inhibition of complement cascade activation by suppressing the expression of a critical low-abundant protein, C2, in the classical complement pathway, significantly improved clinical and immunopathological manifestations of EAMG. Two weeks after a second booster immunization with acetylcholine receptor, when mice exhibit muscle weakness, i.p. injection of C2 siRNA significantly suppressed C2 mRNA in the blood cells and liver of EAMG mice. Treatment of EAMG mice with C2 siRNA, once a week for 5 weeks, significantly improved muscle strength, which was further evidenced by functional AChR preservat...
Source: Journal of Autoimmunity - February 11, 2013 Category: Allergy & Immunology Authors: Huda R, Tüzün E, Christadoss P Tags: J Autoimmun Source Type: research

A procedure for efficient non-viral siRNA transfection of primary human monocytes using nucleofection.
Abstract Monocytes are an important constituent of the innate immune system. Therefore, manipulating gene expression of primary human monocytes is a crucial mean to study and characterize the functions of targeted proteins in monocytes. Gene silencing by transfection of cells with small interfering RNA (siRNA) leading to degradation of the corresponding mRNA and thus to reduced target protein levels is an important tool to investigate gene and protein function of interest. However, non-viral transfection of primary monocytes is challenging because siRNA uptake by these suspended cells is tricky, the individual cel...
Source: Journal of Immunological Methods - April 16, 2015 Category: Allergy & Immunology Authors: Scherer O, Maeß MB, Lindner S, Garscha U, Weinigel C, Rummler S, Werz O, Lorkowski S Tags: J Immunol Methods Source Type: research

Nanotherapy Silencing the Interleukin-8 Gene Produces Regression of Prostate Cancer by Inhibition of Angiogenesis.
This study examines the role of IL-8 in the pathogenesis of metastatic prostate cancer. We developed a biocompatible, cationic polylactide (CPLA) nanocarrier to complex with and efficiently deliver IL-8 siRNA to CaP cells in vitro and in vivo. CPLA IL-8 siRNA nanocomplexes (nanoplexes) protect siRNA from rapid degradation, are nontoxic, have a prolonged lifetime in circulation, and their net positive charge facilitates penetration of cell membranes and subsequent intracellular trafficking. Administration of CPLA IL-8 siRNA nanoplexes to immunodeficient mice bearing human CaP tumors produced significant antitumor activities...
Source: Immunology - May 8, 2016 Category: Allergy & Immunology Authors: Aalinkeel R, Nair B, Chen CK, Mahajan SD, Reynolds JL, Zhang H, Sun H, Sykes DE, Chadha KC, Turowski SG, Bothwell KD, Seshadri M, Chen C, Schwartz SA Tags: Immunology Source Type: research

RNAi-based immunity in insects against baculoviruses and the strategies of baculoviruses involved in siRNA and miRNA pathways to weaken the defense
Dev Comp Immunol. 2021 May 12:104116. doi: 10.1016/j.dci.2021.104116. Online ahead of print.ABSTRACTProtection against viral infection in hosts concerns diverse cellular and molecular mechanisms, among which RNA interference (RNAi) response is a vital one. Small interfering RNAs (siRNAs), microRNAs (miRNAs) and PIWI interacting RNAs (piRNAs) are primary categories of small RNAs involved in RNAi response, playing significant roles in restraining viral invasion. However, during a long-term coevolution, viruses have gained the ability to evade, avoid, or suppress antiviral immunity to ensure efficient replication and transmis...
Source: Developmental and Comparative Immunology - May 15, 2021 Category: Allergy & Immunology Authors: Shudi Zhao Xiangshuo Kong Xiaofeng Wu Source Type: research