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Drug: Melatonin
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Total 4647 results found since Jan 2013.
siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells
Methods Mol Biol. 2022;2550:113-121. doi: 10.1007/978-1-0716-2593-4_15.ABSTRACTSuspension primary cultures of rat pineal cells have been used for decades to determine biochemical regulatory mechanisms of pineal melatonin synthesis, but more recently, RNA interference technology has made the study of the role of specific genes in this melatonin-proficient model system possible. We here present a protocol for preparing rat pineal cell cultures and efficiently knock down gene expression by use of synthetic siRNA.PMID:36180684 | DOI:10.1007/978-1-0716-2593-4_15
Source: Mol Biol Cell - September 30, 2022 Category: Molecular Biology Authors: Aurea Susana Blancas-Velazquez Martin Fredensborg Rath Source Type: research
Question 1: In a child with epilepsy, does melatonin affect the seizure threshold?
Structured clinical question In a school-age child with epilepsy (patient), does melatonin (intervention) affect the seizure threshold (outcome)? Search strategy Search terms: ‘epilepsy’, ‘melatonin’, Primary search, Embase, Medline, Secondary search, The Cochrane library. Search outcome The Cochrane library revealed one relevant article.1 For Embase and Medline, under resources and NHS evidence healthcare databases’ advance search was carried out. First the Embase was searched by using the word ‘Epilepsy’ and ‘epileps*’. The word epileps*, was exploded. Both the words ...
Source: Archives of Disease in Childhood - July 11, 2013 Category: Pediatrics Authors: Banerjee, S., Kumar, V. Tags: ADC Archimedes, Drugs: CNS (not psychiatric), Epilepsy and seizures, Sleep disorders (neurology), Adolescent health, Child health, Infant health, Sleep disorders, Sleep disorders (respiratory medicine) Source Type: research
Chronobiology of mood disorders
ConclusionA preliminary multifaceted model is presented, which will require further development and testing. Future depression research should aim to examine multiple systems concurrently in order to derive a more sophisticated understanding of the underlying neurobiology.
Source: Acta Psychiatrica Scandinavica - August 2, 2013 Category: Psychiatry Authors: G. S. Malhi, S. Kuiper Tags: Clinical Overview Source Type: research
A pilot study to search possible mechanisms of ultralong gonadotropin-releasing hormone agonist therapy in IVF-ET patients with endometriosis
Conclusions:
Ultralong GnRHa therapy reduces the detrimental effects of cytotoxic cytokines and oxidative stress in the ovary in patients with endometriosis.
Source: Journal of Ovarian Research - October 21, 2014 Category: Cancer & Oncology Authors: Hiroshi TamuraAkihisa TakasakiYasuhiko NakamuraFumitaka NumaNorihiro Sugino Source Type: research
Non-canonical Notch Signaling Regulates Actin Remodeling in Cell Migration by Activating PI3K/AKT/Cdc42 Pathway
In conclusion, our research results indicate that DAPT activates PI3K/AKT/Cdc42 signaling by non-canonical Notch pathway, and the activated Cdc42 promotes the filopodia formation and inhibits lamellipodia assembly, resulting in reduced migration of breast cancer cells. The results imply that non-canonical Notch signaling may play a very important role in the rapid response of cells to the extracellular signals.
Author Contributions
LG, JD, and LL designed the study and wrote and revised the manuscript. LL and LZ performed most of the experiments and data analysis. SZ, X-YZ, P-XM, Y-DM, Y-YW, YC, S-JT, and Y-JZ assisted i...
Source: Frontiers in Pharmacology - April 15, 2019 Category: Drugs & Pharmacology Source Type: research
Systems Biology Approaches and Precision Oral Health: A Circadian Clock Perspective
Conclusion
Most head and neck pathologies show a broad cellular heterogeneity making it difficult to achieve an accurate diagnosis and efficient treatment (Graf and Zavodszky, 2017; Lo Nigro et al., 2017). Single cell analysis of circadian omics (Lande-Diner et al., 2015; Abraham et al., 2018), may be a crucial tool needed in the future to fully understand the circadian control of head and neck diseases. It becomes more obvious that there is only a small genetic component but a largely unknown epigenetics and/or environmental component for most of the head and neck pathologies (Moosavi and Motevalizadeh Ardekani, 2016; He...
Source: Frontiers in Physiology - April 15, 2019 Category: Physiology Source Type: research
Role of BMAL1 and CLOCK in regulating the secretion of melatonin in chick retina under monochromatic green light.
Abstract
As the circadian pacemaker of birds, the retina possesses the ability to receive light information, generate circadian oscillation, and secrete melatonin. Previous studies have confirmed that monochromatic green light can accelerate the circadian rhythmic expression of clock genes in the chick retina, thereby increasing cAanat mRNA level and melatonin secretion. However, as the core components of the transcriptional-translational negative feedback loop, the role that cBmal1 and cClock plays in the regulation of the retinal molecular clock system and melatonin secretion under monochromatic green light is u...
Source: Chronobiology International - October 28, 2020 Category: Biology Authors: Bian J, Wang Z, Dong Y, Cao J, Chen Y Tags: Chronobiol Int Source Type: research
JAK2/STAT3 activation by melatonin attenuates the mitochondrial oxidative damage induced by myocardial ischemia/reperfusion injury
This study investigated the protective effect of melatonin pre‐treatment on myocardial IRI and elucidated its potential mechanism. Perfused isolated rat hearts and cultured neonatal rat cardiomyocytes were exposed to melatonin in the absence or presence of the JAK2/STAT3 inhibitor AG490 or JAK2 siRNA and then subjected to IR. Melatonin conferred a cardio‐protective effect, as shown by improved post‐ischemic cardiac function, decreased infarct size, reduced apoptotic index, diminished lactate dehydrogenase release, up‐regulation of the anti‐apoptotic protein Bcl2 and down‐regulation of the pro‐apoptotic protei...
Source: Journal of Pineal Research - May 1, 2013 Category: Research Authors: Yang Yang, Weixun Duan, Zhenxiao Jin, Wei Yi, Juanjuan Yan, Song Zhang, Ning Wang, Zhenxing Liang, Yue Li, Wensheng Chen, Shiqiang Yu, Dinghua Yi Tags: Original Article Source Type: research
Melatonin promotes cardiomyocyte proliferation and heart repair in mice with myocardial infarction via miR-143-3p/Yap/Ctnnd1 signaling pathway.
In this study, we investigated whether melatonin regulated cardiomyocyte proliferation and promoted cardiac repair in mice with myocardial infarction (MI), which was induced by ligation of the left anterior descending coronary artery. We showed that melatonin administration significantly improved the cardiac functions accompanied by markedly enhanced cardiomyocyte proliferation in MI mice. In neonatal mouse cardiomyocytes, treatment with melatonin (1 μM) greatly suppressed miR-143-3p levels. Silencing of miR-143-3p stimulated cardiomyocytes to re-enter the cell cycle. On the contrary, overexpression of miR-143-3p inhibi...
Source: Acta Pharmacologica Sinica - August 23, 2020 Category: Drugs & Pharmacology Authors: Ma WY, Song RJ, Xu BB, Xu Y, Wang XX, Sun HY, Li SN, Liu SZ, Yu MX, Yang F, Ye DY, Gong R, Han ZB, Yu Y, Bamba D, Wang N, Pan ZW, Cai BZ Tags: Acta Pharmacol Sin Source Type: research
Melatonin Inhibits OGD/R-Induced H9c2 Cardiomyocyte Pyroptosis via Regulation of MT2/miR-155/FOXO3a/ARC Axis
Int Heart J. 2022;63(2):327-337. doi: 10.1536/ihj.21-571.ABSTRACTEmerging literature suggests that pyroptosis plays a critical role in ischemia/hypoxia (I/R) -induced myocardial injury. Melatonin has been implicated in attenuating I/R-induced injury of cardiomyocytes. Nevertheless, whether melatonin inhibits I/R-induced pyroptosis of cardiomyocytes and the underlying molecular mechanisms remain unexploited.H9c2 cardiomyocytes were cultured under oxygen-glucose deprivation/reperfusion (OGD/R) condition to establish a myocardial pyroptosis model in vitro. OGD/R-induced pyroptosis was evaluated by CCK-8 assay, IL-1β and IL-1...
Source: International Heart Journal - March 31, 2022 Category: Cardiology Authors: Rui Chen Min Yang Source Type: research
