Filtered By:
Procedure: PET Scan

This page shows you your search results in order of relevance. This is page number 2.

Order by Relevance | Date

Total 89204 results found since Jan 2013.

Effect of thermal and shear stresses in the spray drying process on the stability of siRNA dry powders
In conclusion, stable siRNA based particles for inhalation purposes could be produced using the spray drying technology.Graphical abstract
Source: International Journal of Pharmaceutics - May 9, 2019 Category: Drugs & Pharmacology Source Type: research

Knockdown of Target Genes by siRNA In Vitro
Methods Mol Biol. 2021;2267:159-163. doi: 10.1007/978-1-0716-1217-0_10.ABSTRACTRNA interference (RNAi) is a cellular process involved in the silencing of genes, which makes RNAi important for observing and understanding the function of specific gene products. Short interfering RNA (siRNA) pathway is a RNAi pathway, where exogenous double stranded RNA is introduced to the cell and cleaved by an endoribonuclease, Dicer, to form siRNA, which interacts with a protein complex to scan mRNAs to bind to its complementary sequence. The binding of the siRNA to its complementary mRNA, the mRNA is cleaved and degraded by the cell, sig...
Source: Mol Biol Cell - March 31, 2021 Category: Molecular Biology Authors: Songhee Back James J Manfredi Source Type: research

Positron Emission Tomography Studies of the Biodistribution, Translocation, and Fate of Poly Allyl Amine ‐Based Carriers for Sirna Delivery by Systemic and Intratumoral Administration.
Positron emission tomography is used to study the fate of polyplexes formed by siRNA and modified polyamines. Polyplexes are prepared with either radiolabeled siRNA or radiolabeled polyamines. By comparing biodistribution patterns of labeled siRNA and labeled polyamines conclusions are driven on the stability of the polyplexes in vivo. AbstractPolyamine-based vectors offer many advantages for gene therapy, but they are hampered by a limited knowledge on their biological fate and efficacy for nucleic acid delivery. The18F radiolabeled siRNA is complexed with poly(allyl amine) hydrochloride (PAH), PEGylated PAH (PAHPEG), or ...
Source: Small - August 4, 2023 Category: Nanotechnology Authors: Cristina Simo, Cristian Salvador, Patrizia Andreozzi, Vanessa Gomez ‐Vallejo, Gabriela Romero, Damien Dupin, Jordi Llop, Sergio E. Moya Tags: Research Article Source Type: research

Diatomite biosilica nanocarriers for siRNA transport inside cancer cells
Conclusion Our studies endorse diatomite nanoparticles as non-toxic nanocarriers for siRNA transport in cancer cells. General significance siRNA is a powerful molecular tool for cancer treatment but its delivery is inefficient due to the difficulty to penetrate the cell membrane. siRNA-diatomite nanoconjugate may be well suited for delivery of therapeutic to cancer cells.
Source: Biochimica et Biophysica Acta (BBA) General Subjects - October 17, 2014 Category: Biochemistry Source Type: research

Phenylboronic acid-functionalized polyamidoamine-mediated Bcl-2 siRNA delivery for inhibiting the cell proliferation
In conclusion, the PPP-mediated Bcl-2 siRNA delivery could potentially be an effective platform for solving the drug resistance and further achieving the combined chemotherapy and gene therapy in tumor treatment. Graphical abstract
Source: Colloids and Surfaces B: Biointerfaces - June 29, 2016 Category: Biochemistry Source Type: research

Effects of HMGA2 siRNA and doxorubicin dual delivery by chitosan nanoparticles on cytotoxicity and gene expression of HT‐29 colorectal cancer cell line
ConclusionIn conclusion, our results revealed that dual delivery of a key gene siRNA and appropriate anticancer drug have great impact on the treatment of colorectal cancer.
Source: Journal of Pharmacy and Pharmacology - June 27, 2016 Category: Drugs & Pharmacology Authors: Homayoon Siahmansouri, Mohammad Hossein Somi, Zohreh Babaloo, Behzad Baradaran, Farhad Jadidi‐Niaragh, Fatemeh Atyabi, Hamed Mohammadi, Majid Ahmadi, Mehdi Yousefi Tags: Research Paper Source Type: research

Nanosystems based on siRNA silencing HuR expression counteract diabetic retinopathy in rat
In conclusion, we demonstrated that siRNA can be efficiently delivered into the rat retina using lipid-based nanocarriers, and some of the lipoplexes loaded with siRNA silencing HuR expression are potential candidates to manage retinal diseases. Graphical abstract
Source: Pharmacological Research - July 27, 2016 Category: Drugs & Pharmacology Source Type: research

Using Two-Fluid Nozzle for Spray Freeze Drying to Produce Porous Powder Formulation of Naked siRNA for Inhalation
In this study, we reported the use of two-fluid nozzle to produce spray freeze dried powder of small interfering RNA (siRNA). The effect of atomization gas flow rate and liquid feed rate were inspected initially using herring sperm DNA (hsDNA) as nucleic acid model. The atomization gas flow rate was found to have a major impact on the aerosol properties. The higher the atomization gas flow rate, the smaller the particle size, the higher the fine particle fraction (FPF). In contrast, the liquid feed rate had very minor effect. Subsequently, spray freeze dried siRNA powder was produced at various atomization gas flow rates. ...
Source: International Journal of Pharmaceutics - September 20, 2018 Category: Drugs & Pharmacology Source Type: research

Enhancing anti-melanoma outcomes in mice using novel chitooligosaccharide nanoparticles loaded with therapeutic survivin-targeted siRNA.
In conclusion, PBA-modified COSs can serve as a promising carrier for siRNA delivery in the field of anti-tumor therapy. PMID: 33220463 [PubMed - as supplied by publisher]
Source: European Journal of Pharmaceutical Sciences - November 18, 2020 Category: Drugs & Pharmacology Authors: Liu X, Chen L, Zhang Y, Xin X, Qi L, Jin M, Guan Y, Gao Z, Huang W Tags: Eur J Pharm Sci Source Type: research

Oligonucleotides —A Novel Promising Therapeutic Option for IBD
Conclusions In this review, we focused on recent and past approaches to test the therapeutic efficacy of oligonucleotide based therapies in IBD. The combining mechanistic mode of oligonucleotide based therapeutics is a targeted action on specific pro-inflammatory molecules, which are over activated in IBD patients and contribute significantly to disease pathogenesis. The proposed high selectivity of the agents is derived from its mode of action, that aims to specifically block certain inflammatory molecular patterns, without a general systemic effect on other molecular targets. It would be important for each oligonucleot...
Source: Frontiers in Pharmacology - April 23, 2019 Category: Drugs & Pharmacology Source Type: research

The Role of Decorin in the Adhesion Process of Treponema Pallidum Subspecies Pallidum to Human Brain Microvascular Endothelial Cells
CONCLUSIONS: Decorin enhances the adhesion of T. pallidum to human brain microvascular endothelial cells, suggesting that decorin may act as one of the receptors regulating the adhesion of T. pallidum to cells. Furthermore, T. pallidum treatment triggers the rearrangement of F-actin in cells, and decorin plays a protective role in this process.PMID:37471666
Source: Alternative Therapies in Health and Medicine - July 20, 2023 Category: Complementary Medicine Authors: Fan Wu Xiaoli Dai Tingyu Xue Fangzhi Du Qianqiu Wang Bin Chen Source Type: research

Comparison of efficiency and cytotoxicity of different transfection reagents in transfecting RIP140-siRNA into Kupffer cells.
CONCLUSION: In Kupffer cells, lentivirus-mediated transfection, as compared with the other two trasnfection reagents, can achieve good transfection efficiency with a relativelty low cytotoxicity, and allows for better controllability and stability of the trasnfectiion conditions. PMID: 26714899 [PubMed - in process]
Source: Journal of Southern Medical University - December 20, 2015 Category: Universities & Medical Training Authors: Li J, Liu ZJ Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

Synthesis of cationic branched tea polysaccharide derivatives for targeted delivery of siRNA to hepatocytes.
Abstract The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues was synthesized and characterized using FTIR and 1H NMR spectroscopy. A nonspecific siRNA (NsiRNA) was used as a model molecule of functional siRNA that could downregulate the over-expressed glycometabolism enzymes in liver. The result of agarose gel electrophoresis confirmed that the CTPSA and NsiRNA could form the stable complexes when their weight ratio was larger than 18. The zeta potentials and sizes the complexes were in the range of +8-+15 mv and 120-150 nm, respectively. ...
Source: International Journal of Biological Macromolecules - May 30, 2018 Category: Biochemistry Authors: Wu S, Li N, Yang C, Yan L, Liang X, Ren M, Yang L Tags: Int J Biol Macromol Source Type: research