Synthesis of cationic branched tea polysaccharide derivatives for targeted delivery of siRNA to hepatocytes.

Synthesis of cationic branched tea polysaccharide derivatives for targeted delivery of siRNA to hepatocytes. Int J Biol Macromol. 2018 May 30;: Authors: Wu S, Li N, Yang C, Yan L, Liang X, Ren M, Yang L Abstract The cationic branched tea polysaccharide (CTPSA) derivative bearing N-acylurea and 3-(dimethylamino)-1-propylamine residues was synthesized and characterized using FTIR and 1H NMR spectroscopy. A nonspecific siRNA (NsiRNA) was used as a model molecule of functional siRNA that could downregulate the over-expressed glycometabolism enzymes in liver. The result of agarose gel electrophoresis confirmed that the CTPSA and NsiRNA could form the stable complexes when their weight ratio was larger than 18. The zeta potentials and sizes the complexes were in the range of +8-+15 mv and 120-150 nm, respectively. The CTPSA/NsiRNA complex was observed as nanoparticles with spherical shape in the sizes of 100 nm approximately using scanning electron microscopy. The CTPSA derivative and the CTPSA/NsiRNA complexes exhibited lower cytotoxicity in HL-7702 cells when compared to the branched PEI (bPEI) and the bPEI/NsiRNA complexes assessed by the Cell Counting Kit-8 assay. The results of flow cytometric analysis and laser confocal microscopy indicated that the CTPSA derivative could effectively targeting transfer the NsiRNA to HL-7702 cells. This work provides a potential approach to promote the CTPSA derivative as a nonviral vector for t...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research