Filtered By:
Therapy: Gene Therapy

This page shows you your search results in order of relevance. This is page number 15.

Order by Relevance | Date

Total 5568 results found since Jan 2013.

The search for a T cell line for testing novel antiviral strategies against HIV-1 isolates of diverse receptor tropism and subtype origin.
Abstract The world-wide HIV epidemic is characterized by increasing genetic diversity with multiple viral subtypes, circulating recombinant forms (CRFs) and unique recombinant forms (URFs). Antiretroviral drug design and basic virology studies have largely focused on HIV-1 subtype B. There have been few direct comparisons by subtype, perhaps due to the lack of uniform and standardized culture systems for the in vitro propagation of diverse HIV-1 subtypes. Although peripheral blood mononuclear cells (PBMCs) are major targets and reservoirs of HIV, PBMCs culturing is relatively difficult and not always reproducible....
Source: Journal of Virological Methods - March 31, 2014 Category: Virology Authors: Herrera-Carrillo E, Paxton WA, Berkhout B Tags: J Virol Methods Source Type: research

The in vitro and vivo effects of nuclear and cytosolic parafibromin expression on the aggressive phenotypes of colorectal cancer cells: a search of potential gene therapy target.
Authors: Zheng HC, Liu JJ, Li J, Wu JC, Yang L, Zhao GF, Zhao X, Jiang HM, Huang KQ, Li ZJ Abstract Down-regulated parafibromin is positively linked to the pathogenesis of parathyroid, lung, breast, ovarian, gastric and colorectal cancers. Here, we found that wild-type (WT) parafibromin overexpression suppressed proliferation, tumor growth, induced cell cycle arrest and apoptosis in colorectal cancer cells (p<0.05), but it was the converse for mutant-type (MT, mutation in nucleus localization sequence) parafibromin (p<0.05). Both WT and MT transfectants inhibited migration and invasion, and caused better diff...
Source: Oncotarget - February 24, 2017 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

One of the Ways Researchers Narrow the Search for Drugs to Slow Aging
Small molecule and drug candidate libraries are huge. Much of modern medical research is a process of screening subsets of those libraries in search of molecules that can produce benefits with minimal side-effects. Usually the output of a successful screen is taken as a starting point for further exploration and molecular tinkering, to improve the effect or minimize undesirable side-effects. The great hope for gene therapy is that it will render all of this largely obsolete by offering ways to directly influence a molecular mechanism to a configurable degree without meaningful side-effects. That remains a way off in the fu...
Source: Fight Aging! - January 18, 2019 Category: Research Authors: Reason Tags: Daily News Source Type: blogs

Fight Aging! Newsletter, June 19th 2017
This study is the first to show that downregulation of PAPP-A expression in adult mice can significantly extend life span. Importantly, this beneficial longevity phenotype is distinct from the dwarfism of long-lived PAPP-A KO, Ames dwarf, Snell dwarf and growth hormone receptor (GHR) KO mice with germ-line mutations. Thus, downregulation of PAPP-A expression joins other treatment regimens, such as resveratrol, rapamycin and dietary restriction, which can extend life span when started in mice as adults. In a recent study, inducible knockdown of the GHR in young adult female mice increased maximal, but not median, lif...
Source: Fight Aging! - June 18, 2017 Category: Research Authors: Reason Tags: Newsletters Source Type: blogs

Current Drug Targeting using siRNAbased Nano Therapeutics for Pulmonary Diseases
Curr Pharm Des. 2022 Jul 27. doi: 10.2174/1381612828666220727144223. Online ahead of print.ABSTRACTThe importance of siRNA in nano drug delivery systems to target important pulmonary disorders such as chronic obstructive pulmonary disease (COPD), asthma, lung cancer, and others is reviewed in this perspective. The great majority of lung illnesses are caused by protein misfolding. As a result, siRNA-based therapies are increasingly being used to target the gene. Given the difficulties of delivering bare siRNA, siRNA protection may ensure its efficacy in gene therapy. These issues could be solved with a nano-based siRNA deli...
Source: Current Pharmaceutical Design - July 27, 2022 Category: Drugs & Pharmacology Authors: Venkateshwaran Krishnaswami Ruckmani Kandasamy Source Type: research

Fight Aging! Newsletter, October 30th 2017
In this study, the researchers showed a causal link between dynamic changes in the shapes of mitochondrial networks and longevity. The scientists used C. elegans (nematode worms), which live just two weeks and thus enable the study of aging in real time in the lab. Mitochondrial networks inside cells typically toggle between fused and fragmented states. The researchers found that restricting the worms' diet, or mimicking dietary restriction through genetic manipulation of an energy-sensing protein called AMP-activated protein kinase (AMPK), maintained the mitochondrial networks in a fused or "youthful" state. In add...
Source: Fight Aging! - October 29, 2017 Category: Research Authors: Reason Tags: Newsletters Source Type: blogs

Silencing of Phospholipase C gamma 2 Promotes Proliferation of Rat Hepatocytes In Vitro
In conclusion, these findings suggest that interference with PLCγ2 expression could relieve the inhibitory effect of PLCγ2 on hepaocyte apoptosis, thus promote proliferation through inactivating PKCδ‐mediated JNK1/2 signaling pathway. This article is protected by copyright. All rights reserved
Source: Journal of Cellular Biochemistry - December 13, 2017 Category: Biochemistry Authors: Xiaoguang Chen, Xuemin Zhu, Yumei Liu, Qiongxia Lv, Jun Ma Tags: Article Source Type: research

Fight Aging! Newsletter, January 29th 2018
In conclusion, the present study demonstrated that TIGIT is a prominent negative immune regulator involved in immunosenescence. This novel finding is highly significant, as targeting TIGIT might be an effective strategy to improve the immune response and decrease age-related comorbidities. Delivery of Extracellular Vesicles as a Potential Basis for Therapies https://www.fightaging.org/archives/2018/01/delivery-of-extracellular-vesicles-as-a-potential-basis-for-therapies/ Here I'll point out a readable open access review paper on the potential use of extracellular vesicles as a basis for therapy: harveste...
Source: Fight Aging! - January 28, 2018 Category: Research Authors: Reason Tags: Newsletters Source Type: blogs

Fight Aging! Newsletter, February 19th 2018
Fight Aging! provides a weekly digest of news and commentary for thousands of subscribers interested in the latest longevity science: progress towards the medical control of aging in order to prevent age-related frailty, suffering, and disease, as well as improvements in the present understanding of what works and what doesn't work when it comes to extending healthy life. Expect to see summaries of recent advances in medical research, news from the scientific community, advocacy and fundraising initiatives to help speed work on the repair and reversal of aging, links to online resources, and much more. This content is...
Source: Fight Aging! - February 18, 2018 Category: Research Authors: Reason Tags: Newsletters Source Type: blogs

Effects of RNA interference combined with ultrasonic irradiation and SonoVue microbubbles on expression of STAT3 gene in keratinocytes of psoriatic lesions
SummaryThe most effective sequence of small interfering RNA (siRNA) silencing STAT3 of psoriatic keratinocytes (KCs) was screened out, and the effects of the most effective siRNA combined with ultrasonic irradiation and SonoVue microbubbles on the expression of STAT3 of KCs and the dose- and time-response were investigated. Three chemically-synthetic siRNAs targeting STAT3 carried by Lipofectamine 3000 were transfected into KCs, and the effects on STAT3 expression were detected, then the most effective siRNA was selected for the subsequent experiments. The negative controls of siRNA (siRNA-NC) labeled with Cy3 carried by L...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - April 1, 2017 Category: Research Source Type: research

RNA interference targeting E637K mutation rescues hERG channel currents and restores its kinetic properties
Conclusion: Our findings illustrated that siRNA can effectively inhibit E637K-hERG protein expression and rescue the dominant-negative effect of this mutation by restoring the kinetic properties of hERG protein channel. It has potential clinical implications with regard to the possibility of using siRNA in the treatment of LQTS.
Source: Heart Rhythm - September 28, 2012 Category: Cardiology Authors: Xiaoli Lu, Xi Yang, Xiaoyan Huang, Chen Huang, Huan Huan Sun, Lihua Jin, Weifeng Xu, Haiyan Mao, Junming Guo, Jianqing Zhou, Jiangfang Lian Tags: Experimental Genetic Source Type: research

Effects of silencing MTA1 gene by RNA interference on invasion and metastasis of endometrial carcinoma.
CONCLUSION: RNAi aiming at MTA1 can effectively inhibit the expression of MTA1 in endometrial carcinoma Ishikawa cells and the effective silence of MTA1 can weaken the invasion and metastasis of Ishikawa cells, which provides a new strategy for gene therapy of endometrial carcinoma and an experimental basis for inhibiting the invasion and metastasis of endometrial carcinoma. PMID: 27048111 [PubMed - in process]
Source: European Journal of Gynaecological Oncology - April 7, 2016 Category: OBGYN Tags: Eur J Gynaecol Oncol Source Type: research

pH-responsive DNA nanomicelles for chemo-gene synergetic therapy of anaplastic large cell lymphoma
Conclusion: DNMs co-loaded with Dox and ALK-specific siRNA exhibited significantly enhanced apoptosis of ALCL K299 cells in vitro and effectively inhibited tumor growth in vivo without obvious toxicity, providing a potential strategy in the development of nanomedicines for synergetic cancer therapy.
Source: Theranostics - October 3, 2020 Category: Molecular Biology Authors: Yuwei Li, Shuzhen Yue, Jingyu Cao, Chengzhan Zhu, Yixiu Wang, Xin Hai, Weiling Song, Sai Bi Tags: Research Paper Source Type: research

RNA interference-based osteoanabolic therapy for osteoporosis by a bone-formation surface targeting delivery system
In this study, we identified casein kinase-2 interacting protein-1 encoding gene (Ckip-1), a negative regulator of bone formation, as an effective target of small interfering RNAs (siRNAs) for improving bone mass. Moreover, an impressive (DSS)6-Liposome (Lipos) nanoparticle system that could target the bone formation surface was synthesized to enhance the delivery of Ckip-1 siRNA to osteogenic lineage cells. The in vitro results confirmed that the (DSS)6-Lipos system could efficaciously improve the intracellular delivery of Ckip-1 siRNA without obvious cell toxicity. The in vivo application of the delivery system showed sp...
Source: Cell Research - March 1, 2022 Category: Cytology Authors: Ye Gao He Xin Bolei Cai Le Wang Qianxin Lv Yan Hou Fuwei Liu Taiqiang Dai Liang Kong Source Type: research