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Modulation of Oxidized Low-density Lipoprotein-Affected Macrophage Efferocytosis by Mitochondrial Calcium Uniporter in a Murine Model
CONCLUSIONS: In this study, we found that MCU inhibition modulated intracytoplasmic Ca2+ concentration, improved impaired Mø efferocytosis, and reduced ROS generation. Macrophage efferocytosis removed apoptotic cells and prevented the release of inflammatory factor and foam cell formation, and this can be a potential new therapeutic target for alleviating atherosclerosis.PMID:37689315 | DOI:10.1016/j.imlet.2023.09.003
Source: Atherosclerosis - September 9, 2023 Category: Cardiology Authors: Na Lu Jun-Fan Zhu He-Fan Lv Hai-Peng Zhang Peng-le Wang Jing-Jing Yang Xian-Wei Wang Source Type: research
Sperm acrosomal released proteome reveals MDH and VDAC3 from mitochondria are involved in acrosome formation during spermatogenesis in Eriocheir sinensis
Gene. 2023 Sep 7:147784. doi: 10.1016/j.gene.2023.147784. Online ahead of print.ABSTRACTAcrosome is inextricably related to membranous organelles. The origin of acrosome is still controversial, one reason is that limited articles were reported about the proteomic analysis of the acrosome. Mitochondrial proteins were found exist in the acrosome, nevertheless, only limited attention has been paid to the function of mitochondrial proteins in the acrosome formation. Eriocheir sinensis sperm has a large acrosome, which makes it an ideal model to study acrosome formation. Here, we firstly compared the rate of acrosome reaction i...
Source: Gene - September 9, 2023 Category: Genetics & Stem Cells Authors: Chao Li Ruifang Yu Huan Liu Jiashan Qiao Fenghao Zhang Shumei Mu Mingshen Guo Han Zhang Yanqin Li Xianjiang Kang Source Type: research
Vascular endothelial growth factor B regulates insulin secretion in β cells of type 2 diabetes mellitus mice via PLCγ and the IP3R‑evoked Ca2 < sup > + < /sup > /CaMK2 signaling pathway
Mol Med Rep. 2023 Oct;28(4):197. doi: 10.3892/mmr.2023.13084. Epub 2023 Sep 8.ABSTRACTVascular endothelial growth factor B (VEGFB) plays a crucial role in glucolipid metabolism and is highly associated with type 2 diabetes mellitus (T2DM). The role of VEGFB in the insulin secretion of β cells remains unverified. Thus, the present study aimed to discuss the effect of VEGFB on regulating insulin secretion in T2DM development, and its underlying mechanism. A high‑fat diet and streptozocin (STZ) were used for inducing T2DM in mice model, and VEGFB gene in islet cells of T2DM mice was knocked out by CRISPR Cas9 and overexpre...
Source: Molecular Medicine - September 8, 2023 Category: Molecular Biology Authors: Yuqi Li Rongrong Li Xu Luo Fang Xu Meizi Yang Lanhui Zheng Qihao Wu Wenguo Jiang Yana Li Source Type: research
The involvement of transient receptor potential channels in mast cell activation by microbubbles
This study was to explore the activation of mast cells by microbubbles, with the focus on transient receptor potential (TRP) channels mediated degranulation and calcium influx. Bone marrow-derived mast cells (BMMCs) were primarily obtained from femurs in mice and induced differentiation for 4 weeks. After the purity identification, BMMCs were contacted by homogeneous microbubbles with the diameter of 1 mm for 1 h. β-hexosaminidase and histamine levels in supernatants were assessed by enzyme-linked immunosorbent assay (ELISA) and the CD63 expression was tested by flow cytometry. The intracellular calcium binding with Fluo-...
Source: Molecular Medicine - September 8, 2023 Category: Molecular Biology Authors: Jia Liu Long Qing Yufei He Qihui Zhu Weigang Xu Jianhua Wu Source Type: research
Ant-nest type porous scaffold with micro-struts consisting of carbonate apatite for promoting bone formation and scaffold resorption
In this study, ant-nest type porous (ANP) scaffolds consisting of CO3 Ap were fabricated using calcium carbonate powder or slurry and two types of polyurethane foam through a dissolution-precipitation reaction. ANP-type, three-dimensional, interconnected porous CO3 Ap scaffolds were fabricated by burning out the struts of polyurethane foams embedded in CaCO3 , followed by compositional transformation from CaCO3 to CO3 Ap. The types of polyurethane foam and impregnation methods of CaCO3 into polyurethane form affected the geometry of the resulting ANP structures. Mechanical and in vivo biological performances of these scaff...
Source: Biomed Res - September 8, 2023 Category: Research Authors: Janice Lay Tin Tan Masaya Shimabukuro Mamiko Kobayashi Ryo Kishida Masakazu Kawashita Kunio Ishikawa Source Type: research
Arsenic fractionation and speciation in different textured soils supplied with farmyard manure and accumulation by sunflower under alkaline calcareous conditions
Environ Sci Pollut Res Int. 2023 Sep 8. doi: 10.1007/s11356-023-29659-3. Online ahead of print.ABSTRACTArsenic (As) is a naturally occurring element that is found in soil, water, and rocks. However, it can also be released into the environment through human activities. Arsenic is considered an environmental hazard because it is toxic to humans and animals and can cause serious health problems. Additionally, As-contaminated soil can limit plant growth and reduce crop yields, leading to economic losses for farmers. So, decreasing metal/metalloid solubility in soil by synthetic and organic amendments leads to better crop prod...
Source: Environmental Science and Pollution Research International - September 8, 2023 Category: Environmental Health Authors: Muhammad Awais Piracha Muhammad Ashraf Sher Muhammad Shahzad Sajid Masood Naeem Akhtar Rizwana Kausar Awais Shakoor Source Type: research
Mechanism of Lingbao Huxin Dan in the treatment of bradyarrhythmia complicated with coronary heart disease: a network pharmacology analysis
CONCLUSION: RBA-CHD may treat BA-CHD by regulating adrenergic receptor beta-1, alpha 1-α adrenergic receptor, calcium voltage-gated channel subunit alpha1 C, alpha-1β-adrenergic receptor, nitric oxide synthase 2, beta-2 adrenergic receptor, voltage-dependent calcium channel subunit alpha-2/delta-1, an- giotensin-converting enzyme, Raf-1 proto-oncogene serine/threonine-protein kinase, and other targets, potentially by affecting adrenergic receptor binding and calcium channel opening, to regulate the activity of cardiomyocytes.PMID:37679988 | PMC:PMC10465840 | DOI:10.19852/j.cnki.jtcm.20230802.001
Source: Journal of Traditional Chinese Medicine - September 8, 2023 Category: Complementary Medicine Authors: Han Huagang L I Ziqiang Ouyang Jingfeng Wang Tianquan Dong Lingyan Cao Junling Source Type: research
Black phosphorus-incorporated novel Ti-12Mo-10Zr implant for multimodal treatment of osteosarcoma
This study is expected to provide effective clinical solutions for problems of difficult bone regeneration and tumor recurrence after tumor resection in patients with bone tumors and to solve a series of medical problems such as poor prognosis and poor postoperative quality of patients life with malignant bone tumors.PMID:37682402 | DOI:10.1007/s10534-023-00533-6
Source: Biometals - September 8, 2023 Category: Biochemistry Authors: Bianyun Cai Leizhen Huang Xueke Zhou Xuan Zhou Kun Lei Meng Han Zilin Zhang Xiaofang Li Guangda Li Source Type: research
Improving Biohydrogen Production by Dark Fermentation of Milk Processing Wastewater by Physicochemical and Enzymatic Pretreatments
Appl Biochem Biotechnol. 2023 Sep 8. doi: 10.1007/s12010-023-04619-2. Online ahead of print.ABSTRACTBiohydrogen is considered an alternative energy reserve. Dark fermentation is one of the important green hydrogen production techniques that utilizes organic waste as raw material. It is a promising bioconversion, easy, not expensive, and cost-effective process. Milk processing wastewater (MPWW) is an organic effluent generated in large volumes on a daily basis and disposed directly into the environment. In this research, the study of biochemical hydrogen potential (BHP) test of MPWW was evaluated and used as substrate (S). ...
Source: Applied Biochemistry and Biotechnology - September 8, 2023 Category: Biochemistry Authors: Esma Mahfouf Bouchareb Kerroum Derbal Rayane Bedri Khaled Slimani Souha Menas Halima Lazreg Feriel Maaref Samir Ouabdelkader Aya Saheb Rokaya Bouaita Raouf Bouchareb Nadir Dizge Source Type: research
Development of mechanistic models for the description of radionuclide retention onto calcareous gravels
Chemosphere. 2023 Sep 6:140097. doi: 10.1016/j.chemosphere.2023.140097. Online ahead of print.ABSTRACTThe retention capacity of natural calcareous gravels used as a filling material between the packages containing radioactive waste materials in the disposal cells was evaluated for Sr, U, Ni, Ag, 14C, 99Tc, 239Pu, 228Th and 152Eu. The thermodynamic calculations performed considering repository conditions indicated that the chemistry of most of these elements was dominated by neutral or cationic species, this is the case of 238Pu 152Eu, 228Th, Ni and Ag. The studied gravels presented high sorption capacities for these cation...
Source: Chemosphere - September 8, 2023 Category: Chemistry Authors: Marta L ópez-García Beno ît Madé Alba Valls Jean-Charles Robinet Lara Duro David Garc ía Source Type: research
The involvement of transient receptor potential channels in mast cell activation by microbubbles
This study was to explore the activation of mast cells by microbubbles, with the focus on transient receptor potential (TRP) channels mediated degranulation and calcium influx. Bone marrow-derived mast cells (BMMCs) were primarily obtained from femurs in mice and induced differentiation for 4 weeks. After the purity identification, BMMCs were contacted by homogeneous microbubbles with the diameter of 1 mm for 1 h. β-hexosaminidase and histamine levels in supernatants were assessed by enzyme-linked immunosorbent assay (ELISA) and the CD63 expression was tested by flow cytometry. The intracellular calcium binding with Fluo-...
Source: J Cell Mol Med - September 8, 2023 Category: Molecular Biology Authors: Jia Liu Long Qing Yufei He Qihui Zhu Weigang Xu Jianhua Wu Source Type: research
Development of mechanistic models for the description of radionuclide retention onto calcareous gravels
Chemosphere. 2023 Sep 6:140097. doi: 10.1016/j.chemosphere.2023.140097. Online ahead of print.ABSTRACTThe retention capacity of natural calcareous gravels used as a filling material between the packages containing radioactive waste materials in the disposal cells was evaluated for Sr, U, Ni, Ag, 14C, 99Tc, 239Pu, 228Th and 152Eu. The thermodynamic calculations performed considering repository conditions indicated that the chemistry of most of these elements was dominated by neutral or cationic species, this is the case of 238Pu 152Eu, 228Th, Ni and Ag. The studied gravels presented high sorption capacities for these cation...
Source: Chemosphere - September 8, 2023 Category: Chemistry Authors: Marta L ópez-García Beno ît Madé Alba Valls Jean-Charles Robinet Lara Duro David Garc ía Source Type: research
Ant-nest type porous scaffold with micro-struts consisting of carbonate apatite for promoting bone formation and scaffold resorption
In this study, ant-nest type porous (ANP) scaffolds consisting of CO3 Ap were fabricated using calcium carbonate powder or slurry and two types of polyurethane foam through a dissolution-precipitation reaction. ANP-type, three-dimensional, interconnected porous CO3 Ap scaffolds were fabricated by burning out the struts of polyurethane foams embedded in CaCO3 , followed by compositional transformation from CaCO3 to CO3 Ap. The types of polyurethane foam and impregnation methods of CaCO3 into polyurethane form affected the geometry of the resulting ANP structures. Mechanical and in vivo biological performances of these scaff...
Source: Cell Research - September 8, 2023 Category: Cytology Authors: Janice Lay Tin Tan Masaya Shimabukuro Mamiko Kobayashi Ryo Kishida Masakazu Kawashita Kunio Ishikawa Source Type: research
The involvement of transient receptor potential channels in mast cell activation by microbubbles
This study was to explore the activation of mast cells by microbubbles, with the focus on transient receptor potential (TRP) channels mediated degranulation and calcium influx. Bone marrow-derived mast cells (BMMCs) were primarily obtained from femurs in mice and induced differentiation for 4 weeks. After the purity identification, BMMCs were contacted by homogeneous microbubbles with the diameter of 1 mm for 1 h. β-hexosaminidase and histamine levels in supernatants were assessed by enzyme-linked immunosorbent assay (ELISA) and the CD63 expression was tested by flow cytometry. The intracellular calcium binding with Fluo-...
Source: Molecular Medicine - September 8, 2023 Category: Molecular Biology Authors: Jia Liu Long Qing Yufei He Qihui Zhu Weigang Xu Jianhua Wu Source Type: research
