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Infectious Disease: Adenoviruses
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Total 599 results found since Jan 2013.
Chinese medicine Tongxinluo increases tight junction protein levels by inducing KLF5 expression in microvascular endothelial cells
Tongxinluo (TXL) is a compound prescription formulated according to the meridian theory of traditional Chinese medicine. It may play an important role in cardiovascular protection by improving endothelial cell function. The aim of present study was to investigate whether endothelial protection with TXL is related to its regulation of tight junction protein expression. Human cardiac microvascular endothelial cells (HCMECs) were cultured and treated with 10−7 mol l−1 angiotensin II (Ang II) and the different doses of TXL; the expression of tight junction proteins occludin, claudin, VE‐cadherin and beta‐catenin wa...
Source: Cell Biochemistry and Function - April 23, 2015 Category: Biochemistry Authors: Li‐Min Li, Bing Zheng, Ruo‐Nan Zhang, Li‐Shuang Jin, Cui‐Ying Zheng, Chang Wang, Pei‐Pei Zhou, Zong‐Wei Guo, Dong Ma, Jin‐Kun Wen Tags: Research Article Source Type: research
Ubiquitin C-terminal hydrolase-L1 increases cancer cell invasion by modulating hydrogen peroxide generated via NADPH oxidase 4.
This study explored the role of ubiquitin C-terminal hydrolase-L1 (UCH-L1) in the production of ROS and tumor invasion. UCH-L1 was found to increase cellular ROS levels and promote cell invasion. Silencing UCH-L1, as well as inhibition of H2O2 generation by catalase or by DPI, a NOX inhibitor, suppressed the migration potential of B16F10 cells, indicating that UCH-L1 promotes cell migration by up-regulating H2O2 generation. Silencing NOX4, which generates H2O2, with siRNA eliminated the effect of UCH-L1 on cell migration. On the other hand, NOX4 overexpressed in HeLa cells happens to be ubiquitinated, and NOX4 following de...
Source: Oncotarget - May 2, 2015 Category: Cancer & Oncology Tags: Oncotarget Source Type: research
Spliced XBP1 promotes macrophage survival and autophagy by interacting with Beclin-1.
This study mainly explores the roles of XBP1 mRNA splicing in bone marrow-derived macrophage autophagy. The present study shows that the transient overexpression of spliced XBP1 via adenovirus-mediated gene transfer induces autophagy and promotes proliferation in bone marrow-derived macrophages via the down-regulation of Beclin-1, but that the sustained overexpression of spliced XBP1 leads to apoptosis. When XBP1 is down-regulated in bone marrow-derived macrophages using siRNA, rapamycin-induced autophagosome formation is ablated. Furthermore, we have detected the overexpression of XBP1 in areas of atherosclerotic plaques ...
Source: Biochemical and Biophysical Research communications - May 27, 2015 Category: Biochemistry Authors: Tian PG, Jiang ZX, Li JH, Zhou Z, Zhang QH Tags: Biochem Biophys Res Commun Source Type: research
Betacellulin ameliorates hyperglycemia in obese diabetic db/db mice
Abstract
We found that administration of a recombinant adenovirus (rAd) expressing betacellulin (BTC) into obese diabetic db/db mice ameliorated hyperglycemia. Exogenous glucose clearance was significantly improved, and serum insulin levels were significantly higher in rAd-BTC-treated mice than rAd-β-gal-treated control mice. rAd-BTC treatment increased insulin/bromodeoxyuridine double-positive cells in the islets, and islets from rAd-BTC-treated mice exhibited a significant increase in the level of G1-S phase-related cyclins as compared with control mice. In addition, BTC treatment increased messenger RNA (mRNA...
Source: Journal of Molecular Medicine - June 13, 2015 Category: Molecular Biology Source Type: research
Betacellulin ameliorates hyperglycemia in obese diabetic db/db mice.
Abstract
We found that administration of a recombinant adenovirus (rAd) expressing betacellulin (BTC) into obese diabetic db/db mice ameliorated hyperglycemia. Exogenous glucose clearance was significantly improved, and serum insulin levels were significantly higher in rAd-BTC-treated mice than rAd-β-gal-treated control mice. rAd-BTC treatment increased insulin/bromodeoxyuridine double-positive cells in the islets, and islets from rAd-BTC-treated mice exhibited a significant increase in the level of G1-S phase-related cyclins as compared with control mice. In addition, BTC treatment increased messenger RNA (mRNA)...
Source: Molecular Medicine - June 14, 2015 Category: Molecular Biology Authors: Oh YS, Shin S, Li HY, Park EY, Lee SM, Choi CS, Lim Y, Jung HS, Jun HS Tags: J Mol Med (Berl) Source Type: research
The molecular interaction of heart LIM protein (HLP) with RyR2 and caveolin-3 is essential for Ca(2+)-induced Ca(2+) release in the heart.
Abstract
The heart LIM protein (HLP) is a LIM-only protein family member that mediates protein-protein interactions. To date, no studies have yet been conducted regarding its function in the heart. In the present study, we have identified that HLP binds the cytosolic region of RyR2 in the heart using a bacterial two-hybrid system, LC-MS/MS, co-immunoprecipitation, and GST-pull down assays. Microscopy revealed that HLP forms a triple complex with RyR2 and caveolin-3. siRNA and adenovirus-mediated KD of HLP decreased the electrically evoked Ca(2+) release from the sarcoplasmic reticulum without directly affecting SE...
Source: Biochemical and Biophysical Research communications - June 9, 2015 Category: Biochemistry Authors: Song DW, Lee KE, Ryu JY, Jeon H, Kim DH Tags: Biochem Biophys Res Commun Source Type: research
P120 catenin attenuates lipopolysaccharide-induced blood-brain barrier dysfunction and inflammatory responses in human brain microvascular endothelial cells.
In this study, we found that p120ctn expression was correlated with an increase in the permeability of BBB and a decrease in the expression of tight-junction proteins in human brain microvascular endothelial cells (HBMECs) after LPS challenge. Transfection with p120ctn small interfering RNA (siRNA) induced disruption of BBB integrity, monocyte migration across BBB and inflammatory responses at basal level and after LPS treatment. Conversely, over-expression of p120ctn with adenovirus significantly ameliorated BBB disruption and inflammatory responses in LPS-treated cells. Mechanistically, up-regulation of p120ctn inhibited...
Source: International Journal of Clinical and Experimental Pathology - June 26, 2015 Category: Pathology Authors: Liu N, Li AL, Zhou XP, Chen Q, Cao W Tags: Int J Clin Exp Pathol Source Type: research
CTRP3 attenuates post-infarct cardiac fibrosis by targeting Smad3 activation and inhibiting myofibroblast differentiation
This study aimed to investigate the effect of CTRP3 on cardiac fibrosis and its underlying mechanism. The myocardial expression of CTRP3 was significantly decreased after myocardial infarction (MI). Adenovirus-delivered CTRP3 supplement attenuated myocardial hypertrophy, improved cardiac function, inhibited interstitial fibrosis, and decreased the number of myofibroblasts post-MI. In cultured adult rat cardiac fibroblasts (CFs), CTRP3 attenuated cell proliferation; migration; and the expression of connective tissue growth factor, collagen I, and collagen III induced by transforming growth factor (TGF)-β1. Moreover, CTRP3 ...
Source: Journal of Molecular Medicine - July 3, 2015 Category: Molecular Biology Source Type: research
Hrd1 and ER-Associated Protein Degradation, ERAD, Are Critical Elements of the Adaptive ER Stress Response in Cardiac Myocytes.
CONCLUSIONS: Hrd1 and ERAD are essential components of the adaptive ER stress response in cardiac myocytes. Hrd1 contributes to preserving heart structure and function in a mouse model of pathological cardiac hypertrophy.
PMID: 26137860 [PubMed - as supplied by publisher]
Source: Circulation Research - July 2, 2015 Category: Cardiology Authors: Doroudgar S, Völkers M, Thuerauf DJ, Khan M, Mohsin S, Respress JL, Wang W, Gude NA, Müller OJ, Wehrens XH, Sussman MA, Glembotski CC Tags: Circ Res Source Type: research
Activation of Notch3 promotes pulmonary arterial smooth muscle cells proliferation via Hes1/p27Kip1 signaling pathway
Publication date: Available online 12 August 2015 Source:FEBS Open Bio Author(s): Yang Song, Yonghong Zhang, Haoxiang Jiang, Yanting Zhu, Lu Liu, Wei Feng, Lan Yang, Yibin Wang, Manxiang Li Activation of the Notch3 cascade is involved in the development of pulmonary arterial hypertension by stimulating the proliferation of vascular smooth muscle cells. However, the detailed molecular mechanisms underlying this effect are still unclear. The present study aims to address this issue. We demonstrated that over-expression of intracellular domain of the Notch3 receptor (NICD3) by adenovirus transfection dramatically i...
Source: FEBS Open Bio - August 13, 2015 Category: Molecular Biology Source Type: research
PGC-1α Limits AngiotensinII-Induced Rat Vascular Smooth Muscle Cells Proliferation via Attenuating NOX1-Mediated Generation of Reactive Oxygen Species.
Abstract
Angiotensin II (AngII)-induced excessive reactive oxygen species (ROS) generation and proliferation of vascular smooth muscle cells (VSMCs) is a critical contributor to the pathogenesis of atherosclerosis. Peroxisome proliferator-activated receptor γ (PPARγ) coactivator-1α (PGC-1α) is involved in the regulation of ROS generation, VSMCs proliferation and energy metabolism. The aim of this study was to investigate whether PGC-1α mediates AngII-induced ROS generation and VSMCs hyperplasia. Our results showed that the protein content of PGC-1α was negatively correlated with an increase in cell prolifera...
Source: Bioscience Reports - August 26, 2015 Category: Biomedical Science Authors: Zhao Q, Zhang J, Wang H Tags: Biosci Rep Source Type: research
Dominant negative Ras attenuates pathological ventricular remodeling in pressure overload cardiac hypertrophy
We examined the possible anti-hypertrophic effect of Ras inhibition in vitro using rat neonatal cardiomyocytes (NRCM) and in vivo in the setting of pressure-overload left ventricular (LV) hypertrophy (POH) in rats. Ras functions were modulated via adenovirus directed gene transfer of active mutant Ras-Val12 or dominant negative mutant N17-DN-Ras (DN-Ras). Ras-Val12 expression in vitro activates NFAT resulting in pro-hypertrophic and cardio-toxic effects on NRCM beating and Z-line organization. In contrast, the DN-Ras was antihypertrophic on NRCM, inhibited NFAT and exerted cardio-protective effects attested by preserved NR...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 4, 2015 Category: Molecular Biology Source Type: research
Role of adenoviruses in obesity
Conclusions: several adenoviruses increase adiposity in animals and are associated with obesity in humans. There are critical gaps in the literature needing further investigation including evaluation of other adenovirus subtypes and better research designs to improve the strength of causal inferences. Copyright © 2015 John Wiley & Sons, Ltd.
Source: Reviews in Medical Virology - July 1, 2015 Category: Virology Authors: Jameson D. Voss, Richard L. Atkinson, Nikhil V. Dhurandhar Tags: Review Source Type: research
Estrogen-Related Receptor γ Plays a Key Role in Vascular Calcification Through the Upregulation of BMP2 Expression.
CONCLUSIONS: The present results indicate that ERRγ plays a key role in vascular calcification by upregulating the BMP2 signaling pathway, suggesting that inhibition of ERRγ is a potential therapeutic strategy for the prevention of vascular calcification.
PMID: 26404484 [PubMed - as supplied by publisher]
Source: Arteriosclerosis, Thrombosis and Vascular Biology - September 24, 2015 Category: Cardiology Authors: Kim JH, Choi YK, Do JY, Choi YK, Ha CM, Lee SJ, Jeon JH, Lee WK, Choi HS, Park KG, Lee IK Tags: Arterioscler Thromb Vasc Biol Source Type: research
Elevated expression of periostin in human osteoarthritic cartilage and its potential role in matrix degradation via matrix metalloproteinase-13 Research Communication
We examined periostin expression by immunohistochemical analysis of lesional and nonlesional cartilage from human and rodent OA knee cartilage. In addition, we used small interfering (si)RNA and adenovirus transduction of chondrocytes to knock down and up-regulate periostin levels, respectively, and analyzed its effect on matrix metalloproteinase (MMP)-13, a disintegrin and MMP with thrombospondin motifs (ADAMTS)-4, and type II collagen expression. We found high periostin levels in human and rodent OA cartilage. Periostin increased MMP-13 expression dose [1–10 µg/ml (EC50 0.5–1 μg/ml)] and time (24&nda...
Source: FASEB Journal - October 1, 2015 Category: Biology Authors: Attur, M., Yang, Q., Shimada, K., Tachida, Y., Nagase, H., Mignatti, P., Statman, L., Palmer, G., Kirsch, T., Beier, F., Abramson, S. B. Tags: Research Communication Source Type: research
