Anticancer drug and ionizing radiation-induced DNA damage differently influences transcription activity and DDR-related stress responses of an endothelial monolayer
In conclusion, cAT-induced formation of DSB and profound activation of prototypical DDR factors is independent of the inhibition of RNA polymerase II-regulated transcription in an endothelial monolayer. We suggest that s formed directly or indirectly following cAT-treatment do not act as comprehensive triggers of superior signaling pathways shutting-down transcription while, at the same time, causing an appreciable stimulation of the DDR. Rather, it appears that distinct cAT-induced DNA lesions elicit diverging signaling pathways, which separately control transcription vs. DDR activity in the endothelium.Graphical abstract...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 14, 2020 Category: Molecular Biology Source Type: research

Alterations and molecular targeting of the GSK-3 regulator, PI3K, in head and neck cancer
This article is part of a Special Issue entitled: GSK-3 and related kinases in cancer, neurological and other disorders edited by James McCubrey, Agnieszka Gizak and Dariusz Rakus. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 14, 2020 Category: Molecular Biology Source Type: research

Role of S100 proteins in health and disease
Publication date: Available online 11 February 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Laura L. Gonzalez, Karin Garrie, Mark D. TurnerAbstractThe S100 family of proteins contains 25 known members that share a high degree of sequence and structural similarity. However, only a limited number of family members have been characterized in depth, and the roles of other members are likely undervalued. Their importance should not be underestimated however, as S100 family members function to regulate a diverse array of cellular processes including proliferation, differentiation, inflammat...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 13, 2020 Category: Molecular Biology Source Type: research

During mitosis ZEB1 switches from being a chromatin-bound epithelial gene repressor, to become a microtubule-associated protein
Publication date: Available online 10 February 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): L. Fouani, M.L.H. Huang, L. Cole, P.J. Jansson, Z. Kovacevic, D.R. RichardsonAbstractMicrotubules are polymers of α/β-tubulin, with microtubule organization being regulated by microtubule-associated proteins (MAPs). Herein, we describe a novel role for the epithelial gene repressor, zinc finger E-box-binding homeobox 1 (ZEB1), that switches from a chromatin-associated protein during interphase, to a MAP that associates with α-, β- and γ-tubulin during mitosis. Additi...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 10, 2020 Category: Molecular Biology Source Type: research

Shear stress stimulates integrin β1 trafficking and increases directional migration of cancer cells via promoting deacetylation of microtubule
Publication date: Available online 8 February 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Kai Tang, Shun Li, Ping Li, Xia Qiong, Rui Yang, Tingting Li, Li Li, Ying Jiang, Xiang Qin, Hong Yang, Chunhui Wu, Fengming You, Youhua Tan, Yiyao LiuAbstractIn egress routes of malignancy, cancer cells are constantly subjected to shear stress imposed by blood/lymph flow. Increasing evidence points toward the regulatory roles of shear stress in tumor cell adhesion and motility. Although it is known that integrin endocytic trafficking governs focal adhesion (FA) turnover and cell migration, the e...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 8, 2020 Category: Molecular Biology Source Type: research

The diversity of ACBD proteins – From lipid binding to protein modulators and organelle tethers
Publication date: Available online 8 February 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Markus Islinger, Joseph L. Costello, Suzan Kors, Eric Soupene, Timothy P. Levine, Frans A. Kuypers, Michael SchraderAbstractMembers of the large multigene family of acyl-CoA binding domain containing proteins (ACBDs) share a conserved motif required for binding of Coenzyme A esterified fatty acids of various chain length. These proteins are present in the three kingdoms of life, and despite their predicted roles in cellular lipid metabolism, knowledge about the precise functions of many ACBD pro...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 8, 2020 Category: Molecular Biology Source Type: research

A synthetic Pur-based peptide binds and alters G-quadruplex secondary structure present in the expanded RNA repeat of C9orf72 ALS/FTD
Publication date: Available online 6 February 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Margaret J. Wortman, Ayuna V. Dagdanova, Andrea M. Clark, Earl W. Godfrey, Steven M. Pascal, Edward M. Johnson, Dianne C. DanielAbstractIncreased Pur-alpha (Pura) protein levels in animal models alleviate certain cellular symptoms of the disease spectrum amyotrophic lateral sclerosis/frontotemporal dementia (ALS/FTD). Pura is a member of the Pur family of evolutionarily conserved guanine-rich polynucleotide binding proteins containing a repeated signature PUR domain of 60–80 amino acids. H...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 7, 2020 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: April 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1867, Issue 4Author(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 5, 2020 Category: Molecular Biology Source Type: research

Wdr70 regulates histone modification and genomic maintenance in fission yeast
Publication date: Available online 30 January 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Ming Zeng, Zizhi Tang, Liandi Guo, Xiaojun Wang, Cong LiuAbstractEukaryotic genomes are packaged into highly condensed chromatin and this repressive chromatin barrier can be overcome by altering the chromatin structure via histone modification enzymes. Here, we report Wdr70 in Schizosaccharomyces pombe (spWdr70) plays important roles in multiple cellular processes including cell cycle progression, chromatin structure and DNA repair. Depletion of Wdr70 gene causes cell cycle delay, hypersensitivi...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 1, 2020 Category: Molecular Biology Source Type: research

Glycogen synthase kinase-3 signaling in Alzheimer's disease
Publication date: Available online 30 January 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Elisabetta Lauretti, Ozlem Dincer, Domenico PraticòAbstractAlzheimer's disease (AD) is the most common form of neurodegenerative disorder with dementia, accounting for approximately 70% of the all cases. Currently, 5.8 million people in the U.S. are living with AD and by 2050 this number is expected to double resulting in a significant socio-economic burden. Despite intensive research, the exact mechanisms that trigger AD are still not known and at the present there is no cure for it. In ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 30, 2020 Category: Molecular Biology Source Type: research

MicroRNAs as major regulators of the autophagy pathway
Publication date: Available online 27 January 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Yunus Akkoc, Devrim GozuacikAbstractAutophagy is a cellular stress response mechanism activation of which leads to degradation of cellular components, including proteins as well as damaged organelles in lysosomes. Defects in autophagy mechanisms were associated with several pathologies (e.g. cancer, neurodegenerative diseases, and rare genetic diseases). Therefore, autophagy regulation is under strict control. Transcriptional and post-translational mechanisms that control autophagy in cells and ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 28, 2020 Category: Molecular Biology Source Type: research

Activation of SNF1/AMPK mediates the mitochondrial derepression, resistance to oxidative stress and increased lifespan of cells lacking the phosphatase Sit4p
Publication date: Available online 25 January 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Clara Pereira, Andreia T. Pereira, Vítor Costa (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 26, 2020 Category: Molecular Biology Source Type: research

Artemisinin and its derivatives target mitochondrial c-type cytochromes in yeast and human cells
Publication date: Available online 25 January 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Anais Laleve, Cristina Panozzo, Inge Kühl, Alexa Bourand-Plantefol, Jelena Ostojic, Abdoulaye Sissoko, Déborah Tribouillard-Tanvier, David Cornu, Angélique Burg, Brigitte Meunier, Marc Blondel, Jerome Clain, Nathalie Bonnefoy, Romain Duval, Geneviève DujardinAbstractArtemisinin and its derivatives kill malaria parasites and inhibit the proliferation of cancer cells. In both processes, heme was shown to play a key role in artemisinin bioactivation. We found that artemisi...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 26, 2020 Category: Molecular Biology Source Type: research

β1-Integrin binding to collagen type 1 transmits breast cancer cells into chemoresistance by activating ABC efflux transporters
Publication date: Available online 25 January 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Fabian Baltes, Vladlena Pfeifer, Katja Silbermann, Julia Caspers, Kathleen Wantoch von Rekowski, Martin Schlesinger, Gerd BendasAbstractMolecular interactions of tumor cells with the microenvironment are regarded as onset of chemotherapy resistance, referred to as cell adhesion mediated drug resistance (CAM-DR). Here we elucidate a mechanism of CAM-DR in breast cancer cells in vitro. We show that human MCF-7 and MDA-MB-231 breast cancer cells decrease their sensitivity towards cisplatin, doxorub...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 26, 2020 Category: Molecular Biology Source Type: research

GSK-3: An important kinase in colon and pancreatic cancers
This article is part of a Special Issue entitled: GSK-3 and related kinases in cancer, neurological and other disorders edited by James McCubrey, Agnieszka Gizak and Dariusz Rakus. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 26, 2020 Category: Molecular Biology Source Type: research

GSK-3β in DNA repair, apoptosis, and resistance of chemotherapy, radiotherapy of cancer
This article is part of a Special Issue entitled: GSK-3 and related kinases in cancer, neurological and other disorders edited by James McCubrey, Agnieszka Gizak and Dariusz Rakus. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 23, 2020 Category: Molecular Biology Source Type: research

Decellularized extracellular matrices derived from cultured cells at stepwise myogenic stages for the regulation of myotube formation
Publication date: Available online 21 January 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Takashi Hoshiba, Natsumi YokoyamaAbstractThe regulation of stem cell differentiation is key for muscle tissue engineering and regenerative medicine. To this end, various substrates mimicking the native extracellular matrix (ECM) have been developed with consideration of the mechanical, topological, and biochemical properties. However, mimicking the biochemical properties of the native ECM is difficult due to its compositional complexity. To develop substrates that mimic the native ECM and its bi...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 21, 2020 Category: Molecular Biology Source Type: research

Glycogen Synthase Kinase-3 and phospholipase C-beta signalling: Roles and possible interactions in myelodysplastic syndromes and acute myeloid leukemia
Publication date: Available online 15 January 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Stefano Ratti, Sara Mongiorgi, Isabella Rusciano, Lucia Manzoli, Matilde Y. FolloAbstractGSK-3 and PLCbeta enzymes are responsible for the regulation of several signalling pathways related to many cellular functions. In hematopoietic cells, GSK-3 deficiency is correlated with an MDS-like phenotype and with leukemogenesis, showing a prognostic potential in AML cells. GSK-3 interacts with Wnt or MAPK signalling, but it is also linked to PI3K/Akt/mTOR pathways to regulate cell proliferation and apo...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 15, 2020 Category: Molecular Biology Source Type: research

Alteration of SC35 localization by transfection reagents
In this report, we show that the transfection reagent, JetPRIME alters the localization of the splicing protein SC35 widely used as a nuclear speckle marker. We demonstrate that transfection of plasmids with JetPRIME leads to enlarged SC35 speckles and SC35 cytoplasmic granules. By contrast, transfection of the same plasmid with Lipofectamine 3000 does not have any effect on SC35 localization. The formation of SC35 cytoplasmic granules by JetPRIME-mediated transfection is independent of exogenous expression by plasmid and although similar in morphology they are distinct from P-bodies and stress granules. This method of tra...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 15, 2020 Category: Molecular Biology Source Type: research

Nuclear accumulation of pyruvate dehydrogenase alpha 1 promotes histone acetylation and is essential for zygotic genome activation in porcine embryos
In conclusion, expression of zygotic origin PDHA1 contributes to porcine ZGA by maintaining histone acetylation in porcine embryos. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 12, 2020 Category: Molecular Biology Source Type: research

Roles for Ca2+ and K+ channels in cancer cells exposed to the hypoxic tumour microenvironment
Publication date: Available online 10 January 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Girault Alban, Ahidouch Ahmed, Ouadid-Ahidouch HalimaAbstractFor twenty years, ion channels have been studied in cancer progression. Several information have been collected about their involvement in cancer cellular processes like cell proliferation, motility and their participation in tumour progression using in-vivo models. Tumour microenvironment is currently the focus of many researches and the highlighting of the relationship between cancer cells and surrounding elements, is expanding. One ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 11, 2020 Category: Molecular Biology Source Type: research

Membrane trafficking of large conductance Ca2+- and voltage-activated K+ (BK) channels is regulated by Rab4 GTPase
Publication date: Available online 8 January 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Xiao-Li Wang, Tong Lu, Xiaojing Sun, Hon-Chi LeeAbstractThe large conductance voltage- and Ca2+-activated K+ (BK) channel is a major ionic determinant of vascular tone, vasodilation, and blood pressure. The activity of BK channels is regulated in part by membrane presentation. Rab GTPase (Rab) regulates important cellular processes, including ion channel membrane trafficking. We hypothesize that Rab4a participates in the regulation of BK channel α-subunit (BK-α) membrane trafficking. ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 9, 2020 Category: Molecular Biology Source Type: research

PAK2 activated by Cdc42 and caspase 3 mediates different cellular responses to oxidative stress-induced apoptosis
In this study, through protein biochemical analyses and various cell-based assays, we demonstrated that full-length PAK2 activated by Cdc42 was resistant to the cleavage by caspase 3 in vitro and within cells. When mammalian cells were treated by oxidative stress using hydrogen peroxide, PAK2 was highly activated through caspase 3 cleavage that led to apoptosis. However, when PAK2 was pre-activated by Cdc42 or by mild stress such as serum deprivation, it was no longer able to be cleaved by caspase 3 upon hydrogen peroxide treatment, and the subsequent apoptosis was also largely inhibited. Furthermore, cells expressing acti...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 9, 2020 Category: Molecular Biology Source Type: research

ISG15 suppresses translation of ABCC2 via ISGylation of hnRNPA2B1 and enhances drug sensitivity in cisplatin resistant ovarian cancer cells
Publication date: Available online 9 January 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Jia-Mei Wang, Bao-Qin Liu, Qi Zhang, Liang Hao, Chao Li, Jing Yan, Fu-Ying Zhao, Huai-Yu Qiao, Jing-Yi Jiang, Hua-Qin WangAbstractCisplatin-based chemotherapies have long been considered as a standard chemotherapy in ovarian cancer. However, cisplatin resistance restricts beneficial therapy for patients with ovarian cancer. The ubiquitin-like protein interferon-stimulated gene 15 (ISG15) encodes a 15-kDa protein, that is implicated in the post-translational modification of diverse proteins. In th...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 9, 2020 Category: Molecular Biology Source Type: research

Claudin-2 binding peptides, VPDSM and DSMKF, down-regulate claudin-2 expression and anticancer resistance in human lung adenocarcinoma A549 cells
Publication date: Available online 7 January 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Haruka Nasako, Risa Akizuki, Yui Takashina, Yoshinobu Ishikawa, Takehiro Shinoda, Mikako Shirouzu, Tomohiro Asai, Toshiyuki Matsunaga, Satoshi Endo, Akira IkariAbstractClaudin-2 (CLDN2), a tight junctional protein, is involved in the chemoresistance in spheroid culture models of human lung adenocarcinoma A549 cells. However, there is no chemical which can improve the sensitivity to anticancer drugs. So far, we reported that DFYSP, a short peptide which mimics the second extracellular loop (ECL2) ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 8, 2020 Category: Molecular Biology Source Type: research

Oxidative stress induces transcription of telomeric repeat-containing RNA (TERRA) by engaging PKA signaling and cytoskeleton dynamics
Publication date: Available online 7 January 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Natalia M. Galigniana, Nancy L. Charó, Romina Uranga, Ana María Cabanillas, Graciela Piwien-PilipukAbstractLong non-coding RNAs transcribed from telomeres, known as TERRA (telomeric repeat-containing RNA), are associated with telomere and genome stability. TERRA abundance responds to different cell stresses; however, no studies have focused on oxidative stress, condition that damages biomolecules and is involved in aging and disease. Since telomeres are prone to oxidative damage lea...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 8, 2020 Category: Molecular Biology Source Type: research

Intraflagellar transport 20: New target for the treatment of ciliopathies
We describe studies related to the role of IFT20 in cilia formation and discuss new targets for treating diseases associated with ciliary dysplasia. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 30, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: February 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1867, Issue 2Author(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 27, 2019 Category: Molecular Biology Source Type: research

Roles of Id1/HIF-1 and CDK5/HIF-1 in cell cycle reentry induced by amyloid-beta peptide in post-mitotic cortical neuron
Publication date: Available online 26 December 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): A-Ching Chao, Chien-Hui Chen, Ming-Hsuan Wu, Bo-Yu Hou, Ding-I YangAbstractOne neurotoxic mechanism of amyloid-beta peptide (Aβ), the major component of senile plaques in the brains of Alzheimer's disease (AD) patients, is to trigger cell cycle reentry in fully differentiated neurons. However, the detailed underlying mechanisms remain unclear. Using Aβ25-35–treated primary rat cortical neurons as the experimental system, in the present study we tested whether Aβ-induced inh...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 26, 2019 Category: Molecular Biology Source Type: research

Crosstalks of GSK3 signaling with the mTOR network and effects on targeted therapy of cancer
Publication date: Available online 26 December 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Camilla Evangelisti, Francesca Chiarini, Francesca Paganelli, Sandra Marmiroli, Alberto M. MartelliAbstractThe introduction of therapeutics targeting specific tumor-promoting oncogenic or non-oncogenic signaling pathways has revolutionized cancer treatment. Mechanistic (previously mammalian) target of rapamycin (mTOR), a highly conserved Ser/Thr kinase, is a central hub of the phosphatidylinositol 3-kinase (PI3K)/Akt/mTOR network, one of the most frequently deregulated signaling pathways in can...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 26, 2019 Category: Molecular Biology Source Type: research

Kinesin-6 family motor KIF20A regulates central spindle assembly and acrosome biogenesis in mouse spermatogenesis
Publication date: Available online 26 December 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Zhen-Yu She, Yue-Ling Li, Yang Lin, Ming-Hui Lu, Ya-Lan Wei, Kai-Wei Yu, Ning Zhong, Yu XiaoAbstractKinesin-6 KIF20A is essential for microtubule organization and central spindle assembly during cytokinesis. However, the functions of KIF20A in meiotic division and spermatogenesis remain elusive. Here, we report that kinesin-6 KIF20A locates at the microtubules in mouse spermatogenic cells and co-localizes with the spindle midzone and midbody. We demonstrate that central spindle organization and...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 26, 2019 Category: Molecular Biology Source Type: research

Multifaceted roles of COPII subunits in autophagy
Publication date: Available online 19 December 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Zhongyu Li, Wei Huang, Wei WangAbstractCOPII vesicles mediate anterograde ER-Golgi traffic of newly synthesized proteins in nutrient rich conditions. An accumulating body of results indicates that the secretory COPII vesicles can be shifted to the roles in autophagosome formation and selective ER-phagy (autophagy of ER), depending on their specific subunits, in response to environmental stresses. In this mini-review, we summarize and discuss the multifaceted roles of COPII vesicles in autophagy...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 20, 2019 Category: Molecular Biology Source Type: research

Neuropeptide bombesin receptor activation stimulates growth of lung cancer cells through HER3 with a MAPK-dependent mechanism
Publication date: Available online 17 December 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Lingaku Lee, Irene Ramos-Alvarez, Terry W. Moody, Samuel A. Mantey, Robert T. JensenAbstractDespite recent advances in treatment of non-small cell lung cancer (NSCLC), prognosis still remains poor and new therapeutic approaches are needed. Studies demonstrate the importance of the EGFR/HER-receptor family in NSCLC growth, as well as that of other tumors. Recently, HER3 is receiving increased attention because of its role in drug resistance and aggressive growth. Activation of overexpressed G-pr...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 18, 2019 Category: Molecular Biology Source Type: research

Cardiac regeneration as an environmental adaptation
Publication date: Available online 12 December 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Akane Sakaguchi, Chihiro Nishiyama, Wataru KimuraAbstractHeart failure is a devastating disease that affects more than 26 million individuals worldwide and has a 5-year survival rate of less than 50%, with its development in part reflecting the inability of the adult mammalian heart to regenerate damaged myocardium. In contrast, certain vertebrate species including fish and amphibians, as well as neonatal mammals, are capable of complete cardiac regeneration after various types of myocardial in...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 13, 2019 Category: Molecular Biology Source Type: research

9th Ascona International Workshop on cardiomyocyte biology
Publication date: Available online 12 December 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Marijke Brink, Christian Zuppinger (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 13, 2019 Category: Molecular Biology Source Type: research

CPSF7 regulates liver cancer growth and metastasis by facilitating WWP2-FL and targeting the WWP2/PTEN/AKT signaling pathway
In this study, we investigated the role of CPSF7 in hepatocellular carcinoma. Experimental evidence suggests that the expression level of CPSF7 is higher in liver cancer cells and tissues than in non-tumor hepatic cells and tissues. Furthermore, knockdown of CPSF7 effectively suppressed cell proliferation, migration and colony formation in liver cancer cells by inhibiting PTEN/AKT signaling. CPSF7 promoted WWP2-FL due to the presence of PTEN ubiquitination sites in this longer transcript. Taken together, we identified that CPSF7 regulates liver cancer growth by targeting WWP2-FL that in turn regulates AKT activation in a P...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 13, 2019 Category: Molecular Biology Source Type: research

Differential use of BTK and PLC in FcεRI- and KIT-mediated mast cell activation: A marginal role of BTK upon KIT activation
Publication date: Available online 11 December 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Anne Simonowski, Thomas Wilhelm, Pardes Habib, Carolin N. Zorn, Michael HuberAbstractIn mast cells (MCs), the TEC family kinase (TFK) BTK constitutes a central regulator of antigen (Ag)-triggered, FcεRI-mediated PLCγ phosphorylation, Ca2+ mobilization, degranulation, and pro-inflammatory cytokine production. Less is known about the function of BTK in the context of stem cell factor (SCF)-induced KIT signaling. In bone marrow-derived MCs (BMMCs), Ag stimulation caused intense phosp...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 11, 2019 Category: Molecular Biology Source Type: research

Palmitate and oleate modify membrane fluidity and kinase activities of INS-1E β-cells alongside altered metabolism-secretion coupling
Publication date: Available online 7 December 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Lucie Oberhauser, Sabrina Granziera, Adai Colom, Antoine Goujon, Vanessa Lavallard, Stefan Matile, Aurélien Roux, Thierry Brun, Pierre MaechlerAbstractChronic exposure to elevated levels of glucose and free fatty acids impairs beta-cell function, leading to insulin secretion defects and eventually beta-cell failure. Using a semi-high throughput approach applied to INS-1E beta-cells, we tested multiple conditions of chronic exposure to basal, intermediate and high glucose, combined with sa...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 7, 2019 Category: Molecular Biology Source Type: research

Enhanced insulin signaling and its downstream effects in iron-overloaded primary hepatocytes from hepcidin knock-out mice
ConclusionsHepatocytes from Hamp1−/− mice showed evidence of greater sensitivity to the effects of insulin than WT hepatocytes. This may explain the insulin-sensitive phenotype that has been reported in classical hemochromatosis.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 6, 2019 Category: Molecular Biology Source Type: research

TRPC5 channel instability induced by depalmitoylation protects striatal neurons against oxidative stress in Huntington's disease
Publication date: Available online 6 December 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Chansik Hong, Seo Hwa Choi, Misun Kwak, Byeongseok Jeong, Juyeon Ko, Hyung Joon Park, Seok Choi, Jae Yeoul Jun, Insuk SoAbstractProtein S-palmitoylation, the covalent lipid modification of the side chain of Cys residues with the 16‑carbon fatty acid palmitate, is the most common acylation, and it enhances the membrane stability of ion channels. This post-translational modification (PTM) determines a functional mechanism of ion channel life cycle from maturation and membrane trafficking to loca...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 6, 2019 Category: Molecular Biology Source Type: research

Lanosterol modulates proteostasis via dissolving cytosolic sequestosomes/aggresome-like induced structures
Publication date: Available online 27 November 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Li-Dan Hu, Jing Wang, Xiang-Jun Chen, Yong-Bin YanAbstractSequestration of misfolded proteins into distinct cellular compartments plays a pivotal role in proteostasis and proteopathies. Cytoplasmic ubiquitinated proteins are sequestered by p62/SQSTM1 to deposit in sequestosomes or aggresome-like induced structures (ALIS). Most aggresome or ALIS regulators identified thus far are recruiters, while little is known about the disaggregases or dissolvers. In this research, we showed that lanosterol ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 28, 2019 Category: Molecular Biology Source Type: research

Emerging roles of GSK-3α in pathophysiology: Emphasis on cardio-metabolic disorders
Publication date: Available online 27 November 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Firdos Ahmad, James R. WoodgettAbstractGlycogen synthase kinase-3 (GSK-3) is a widely expressed serine/threonine kinase regulates a variety of cellular processes including proliferation, differentiation and death. Mammals harbor two structurally similar isoforms GSK-3α and β that have overlapping as well as unique functions. Of the two, GSK-3β has been studied (and reviewed) in far greater detail with analysis of GSK-3α often as an afterthought. It is now evident that syst...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 28, 2019 Category: Molecular Biology Source Type: research

Novel PRMT5-mediated arginine methylations of HSP90A are essential for maintenance of HSP90A function in NDRG2low ATL and various cancer cells
Publication date: Available online 22 November 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Tomonaga Ichikawa, Obeid Shanab, Shingo Nakahata, Shunsuke Shimosaki, Nawin Manachai, Masaya Ono, Hidekatsu Iha, Kazuya Shimoda, Kazuhiro MorishitaAbstractN-myc downstream-regulated gene 2 (NDRG2) as a tumor suppressor is frequently downregulated in human T-lymphotropic retrovirus (HTLV-1)-infected adult T-cell leukemia (ATL) and variety of cancers, and negatively regulates PI3K signaling pathways through dephosphorylation of PTEN with protein phosphatase 2A (PP2A). We recently identified that ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 24, 2019 Category: Molecular Biology Source Type: research

Aerobic glycolysis is a metabolic requirement to maintain the M2-like polarization of tumor-associated macrophages
Publication date: Available online 21 November 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Natália M. de-Brito, Julia Duncan-Moretti, Hayandra C. da-Costa, Roberta Saldanha-Gama, Heitor A. Paula-Neto, Gabriel Dorighello, Rafael L. Simões, Christina Barja-FidalgoAbstractMacrophages (MO) are versatile cells, assuming distinct functional phenotypes depending on the activating stimulus and the microenvironment. The differential activation of macrophages is supported by profound intracellular metabolic changes, being well accepted that the M1/M(LPS+IFN-γ) phenotype rel...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 21, 2019 Category: Molecular Biology Source Type: research

Multiscale simulation unravel the kinetic mechanisms of inflammasome assembly
Publication date: Available online 21 November 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Zhaoqian Su, Yinghao WuAbstractIn the innate immune system, the host defense from the invasion of external pathogens triggers the inflammatory responses. Proteins involved in the inflammatory pathways were often found to aggregate into supramolecular oligomers, called ‘inflammasome’, mostly through the homotypic interaction between their domains that belong to the death domain superfamily. Although much has been known about the formation of these helical molecular machineries, the d...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 21, 2019 Category: Molecular Biology Source Type: research

Molecular insight on the altered membrane trafficking of TrkA kinase dead mutants
In conclusion, our results allow to distinguish two different TrkA membrane immobilization modes and demonstrate that not all kinase-inactive mutants display identical membrane trafficking. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 21, 2019 Category: Molecular Biology Source Type: research

Regulation of the autophagic PI3KC3 complex by laforin/malin E3-ubiquitin ligase, two proteins involved in Lafora disease
Publication date: Available online 21 November 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Pablo Sanchez-Martin, Marcos Lahuerta, Rosa Viana, Erwin Knecht, Pascual SanzAbstractLafora progressive myoclonus epilepsy is a fatal rare neurodegenerative disorder characterized by the accumulation of insoluble abnormal glycogen deposits in the brain and peripheral tissues. Mutations in at least two genes are responsible for the disease: EPM2A, encoding the glucan phosphatase laforin, and EPM2B, encoding the RING-type E3-ubiquitin ligase malin. Both laforin and malin form a functional complex...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 21, 2019 Category: Molecular Biology Source Type: research

DHEAS prevents pro-metastatic and proliferative effects of 17ß-estradiol on MCF-7 breast cancer cells
Publication date: Available online 21 November 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Neha Upmanyu, Ahmed Bulldan, Klaus Failing, Georgios Scheiner-BobisAbstractIt is generally assumed that circulating dehydroepiandrosterone sulfate (DHEAS) can be desulfated and further metabolized to estrogen, which is of concern for all patients with estrogen-responsive breast cancer. We addressed this issue by comparing the effects of DHEAS, its desulfated form DHEA, and 17ß-estradiol on human metastatic, estrogen-responsive MCF-7 breast cancer cells.Physiological concentrations of DHEA...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 21, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: January 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1867, Issue 1Author(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 21, 2019 Category: Molecular Biology Source Type: research

Reviewer Acknowledgement
Publication date: January 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1867, Issue 1Author(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 21, 2019 Category: Molecular Biology Source Type: research