Corrigendum to “PUMA dependent mitophagy by Abrus agglutinin contributes to apoptosis through ceramide generation” [Biochim. Biophys. Acta Mol. Cell Res. 1865 (2018) 480–495]
Publication date: Available online 16 May 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Prashanta Kumar Panda, Prajna Paramita Naik, Biswa Ranjan Meher, Durgesh Nandini Das, Subhadip Mukhopadhyay, Prakash Priyadarshi Praharaj, Tapas K. Maiti, Sujit K. Bhutia (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - May 16, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: August 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1866, Issue 8Author(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - May 12, 2019 Category: Molecular Biology Source Type: research

SIRT7 regulates the nuclear export of NF-κB p65 by deacetylating Ran
In this study, we demonstrated that SIRT7 interacts with a small GTPase, Ras-related nuclear antigen (Ran), and deacetylates Ran at K37. The nuclear export of p65 was facilitated in SIRT7-deficient fibroblast cells, while the nuclear export was inhibited in SIRT7-deficient cells expressing K37R-Ran (deacetylation-mimicking mutant). Additionally, the nuclear export of p65 in wild-type fibroblast cells was promoted by K37Q-Ran (acetylation-mimicking mutant). K37Q-Ran exhibited an increased ability to bind to chromosome region maintenance 1 (CRM1), which is a major nuclear receptor that mediates the export of cargo proteins, ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - May 7, 2019 Category: Molecular Biology Source Type: research

Differential intracellular localization and dynamic nucleocytoplasmic shuttling of homeodomain-interacting protein kinase family members
Publication date: Available online 25 April 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Olesja Ritter, M. Lienhard SchmitzAbstractThe three canonical members of the family of homeodomain-interacting protein (HIP) kinases fulfill overlapping and distinct roles in cellular stress response pathways. Here we systematically compared all three endogenous HIPKs for their intracellular distribution and mutual interactions. The endogenous HIPKs are contained in high molecular weight complexes of ~700 kDa but do not directly interact physically. Under basal conditions, HIPK1 was mostly cytop...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - April 26, 2019 Category: Molecular Biology Source Type: research

A structural prospective for collagen receptors such as DDR and their binding of the collagen fibril
Publication date: Available online 18 April 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Joseph P.R.O. Orgel, Rama S. MadhurapantulaAbstractThe structure of the collagen fibril surface directly effects and possibly assists the management of, collagen receptor interactions. An important class of collagen receptors, the receptor tyrosine kinases of the Discoidin Domain Receptor family (DDR1 and DDR2), are differentially activated by specific collagen types and play important roles in cell adhesion, migration, proliferation, and matrix remodeling. This review discusses their structure an...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - April 19, 2019 Category: Molecular Biology Source Type: research

Tumor protein D52 (isoform 3) interacts with and promotes peroxidase activity of Peroxiredoxin 1 in prostate cancer cells implicated in cell growth and migration
Publication date: Available online 11 April 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Chandrashekhar Dasari, Karthik Reddy Kami Reddy, Sirisha Natani, T.R.L. Murthy, Supriya Bhukya, Ramesh UmmanniAbstractTumor protein D52 (TPD52) is overexpressed in multiple cancers including prostate cancer due to gene amplification and investigations to understand its role in the pathophysiology of different cancers are continuing. GST pull-down assays and Tandem affinity purification of TPD52 as bait identified novel prey Peroxiredoxin 1 (PRDX1) in prostate cancer (PCa) cells. PRDX1 interaction ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - April 13, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: July 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1866, Issue 7Author(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - April 10, 2019 Category: Molecular Biology Source Type: research

Cannabinoid-sensitive receptors in cardiac physiology and ischaemia
This article is part of a Special Issue entitled: Cardiomyocyte biology: new pathways of differentiation and regeneration edited by Marijke Brinkm, Marcus C. Schaub, and Christian Zuppinger. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - April 7, 2019 Category: Molecular Biology Source Type: research

Dichotomy of the function of DDR1 in cells and disease progression
Publication date: Available online 5 April 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Yi-Chun Yeh, Hsi-Hui Lin, Ming-Jer TangAbstractDiscoidin domain receptors DDR1 and DDR2 are collagen receptor tyrosine kinases that have many roles in tissue development and disease progression. Under physiological conditions, DDR1 is predominantly expressed in epithelial cells and functions to maintain cell differentiation and tissue homeostasis. A switch in expression from DDR1 to DDR2 occurs during epithelial-to-mesenchymal transition. However, opposite effects of DDR1 are reported to be involve...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - April 7, 2019 Category: Molecular Biology Source Type: research

A new human calpastatin skipped of the inhibitory region protects calpain-1 from inactivation and degradation
Publication date: Available online 5 April 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Bianca Sparatore, Marco Pedrazzi, Anna Garuti, Alice Franchi, Monica Averna, Alberto Ballestrero, Roberta De TullioAbstractSeveral human acute and chronic diseases involve calpain over-activation. However, the mechanistic linkages between the etiology and the progression of cell damages are not yet completely understood. Here we show that different human cells and tissues, including brain tumor specimens, cell lines of nerve origin, breast tumor samples and peripheral blood mononuclear cells from h...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - April 7, 2019 Category: Molecular Biology Source Type: research

DDR1 role in fibrosis and its pharmacological targeting
Publication date: Available online 5 April 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Solange Moll, Alexis Desmoulière, Marcus J. Moeller, Jean-Claude Pache, Laura Badi, Filippo Arcadu, Hans Richter, Alexander Satz, Sabine Uhles, Andrea Cavalli, Faye Drawnel, Leonardo Scapozza, Marco PrunottoAbstractDiscoidin domain receptor1 (DDR1) is a collagen activated receptor tyrosine kinase and an attractive anti-fibrotic target. Its expression is mainly limited to epithelial cells located in several organs including skin, kidney, liver and lung. DDR1's biology is elusive, with unknown...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - April 7, 2019 Category: Molecular Biology Source Type: research

P53/NRF2 mediates SIRT1's protective effect on diabetic nephropathy
Publication date: Available online 6 April 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Fuzhe Ma, Junduo Wu, Ziping Jiang, Wenlin Huang, Ye Jia, Weixia Sun, Hao WuAbstractDiabetic nephropathy (DN) is the leading cause of end stage renal disease, posing a severe threat to public health. Previous studies reported the protective role of sirtuin 1 (SIRT1) in DN, encouraging the investigation of more potent and specific SIRT1 activators. SRT2104 is a novel, first-in-class, highly selective small-molecule activator of SIRT1, with its effect and mechanism unknown on DN. To this end, streptoz...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - April 7, 2019 Category: Molecular Biology Source Type: research

Human pluripotent stem cell-derived cardiomyocytes for studying energy metabolism
This article is part of a Special Issue entitled: Cardiomyocyte biology: new pathways of differentiation and regeneration edited by Marijke Brinkm, Marcus C. Schaub, and Christian Zuppinger. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - April 5, 2019 Category: Molecular Biology Source Type: research

Getting a grip on adhesion: Cadherin switching and collagen signaling
Publication date: Available online 5 April 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Huocong Huang, Steven Wright, Junqiu Zhang, Rolf A. BrekkenAbstractEpithelial-mesenchymal transition (EMT) is a developmental biological process that is hijacked during tumor progression. Cadherin switching, which disrupts adherens junctions and alters cadherin-associated signaling pathways, is common during EMT. In many tumors, substantial extracellular matrix (ECM) is deposited. Collagen is the most abundant ECM constituent and it mediates specific signaling pathways by binding to integrins and d...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - April 5, 2019 Category: Molecular Biology Source Type: research

Corrigendum to “HOTAIR/miR-326/FUT6 axis facilitates colorectal cancer progression through regulating fucosylation of CD44 via PI3K/AKT/mTOR pathway” [BBA – Mol. Cell Res. 1866 (2019) 750–760]
Publication date: Available online 4 April 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Shimeng Pan, Yanqiu Liu, Qianqian Liu, Yang Xiao, Bing Liu, Xiang Ren, Xia Qi, Huimin Zhou, Changqian Zeng, Li Jia (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - April 5, 2019 Category: Molecular Biology Source Type: research

MURC/CAVIN-4 facilitates store-operated calcium entry in neonatal cardiomyocytes
Publication date: Available online 2 April 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Julien Malette, Jade Degrandmaison, Hugo Giguère, Jonathan Berthiaume, Maude Frappier, Jean-Luc Parent, Mannix Auger-Messier, Guylain BoulayAbstractIntact store-operated calcium entry (SOCE) mechanisms ensure the maintenance of Ca2+ homeostasis in cardiomyocytes while their dysregulation promotes the development of cardiomyopathies. To better understand this calcium handling process in cardiomyocytes, we sought to identify unknown protein partners of stromal interaction molecule 1 (STIM1), a...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - April 3, 2019 Category: Molecular Biology Source Type: research

tRNAs and tRNA fragments as modulators of cardiac and skeletal muscle function
This article is part of a Special Issue entitled: Cardiomyocyte biology: new pathways of differentiation and regeneration edited by Marijke Brinkm, Marcus C. Schaub, and Christian Zuppinger. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - April 1, 2019 Category: Molecular Biology Source Type: research

The circadian protein CLOCK regulates cell metabolism via the mitochondrial carrier SLC25A10
In conclusion, we identified a new protein-protein interaction mechanism in which CLOCK can directly regulate cell metabolism via the mitochondrial membrane transporter SLC25A10. Our study might provide some new insights into the relationship between circadian clock and mitochondrial metabolism. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - April 1, 2019 Category: Molecular Biology Source Type: research

Oxygen as a key regulator of cardiomyocyte proliferation: New results about cell culture conditions!
Publication date: Available online 15 March 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Anne-Charlotte Bon-Mathier, Stéphanie Rignault-Clerc, Christelle Bielmann, Nathalie Rosenblatt-VelinAbstractThe goal of the new therapeutically strategies aimed to treat cardiovascular diseases (CVDs) is to enhance the natural ability of the heart to regenerate. This represents a great challenge for the coming years as all the mechanisms underlying the replacement of dying cells by functional cells of the same type are not completely elucidated. Among these, stimulating cardiomyocyte prolif...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 31, 2019 Category: Molecular Biology Source Type: research

Identification of two independent SUMO-interacting motifs in Fas-associated factor 1 (FAF1): Implications for mineralocorticoid receptor (MR)-mediated transcriptional regulation
Publication date: Available online 29 March 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Chi-Hsien Wang, Pei-Wen Hung, Chi-Wu Chiang, Marc Lombès, Chang-Han Chen, Kuen-Haur Lee, Yu-Chih Lo, Mei-Hsiang Wu, Wen-Chang Chang, Ding-Yen LinAbstractFas-associated factor 1 (FAF1) was originally isolated as a Fas-associated factor and was subsequently found to interact with numerous other proteins that are involved in various cellular events including Fas-mediated apoptosis, nuclear factor (NF)-κB, Wnt/β-catenin, and transforming growth factor (TGF)-β signaling pathways,...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 30, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: June 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1866, Issue 6Author(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 30, 2019 Category: Molecular Biology Source Type: research

Advances in understanding the mechanisms of evasive and innate resistance to mTOR inhibition in cancer cells
Publication date: Available online 27 March 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Francesca Charini, Camilla Evangelisti, Giovanna Lattanzi, James A. McCubrey, Alberto M. MartelliAbstractThe development of drug-resistance by neoplastic cells is recognized as a major cause of targeted therapy failure and disease progression. The mechanistic (previously mammalian) target of rapamycin (mTOR) is a highly conserved Ser/Thr kinase that acts as the catalytic subunit of two structurally and functionally distinct large multiprotein complexes, referred to as mTOR complex 1 (mTORC1) and m...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 28, 2019 Category: Molecular Biology Source Type: research

Cardiomyocyte proliferation, a target for cardiac regeneration
Publication date: Available online 28 March 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Sandy M. Payan, Fabien Hubert, Francesca RochaisAbstractCardiac diseases, characterized by cardiomyocyte loss, lead to dramatic impairment of cardiac function and ultimately to congestive heart failure. Despite significant advances, conventional treatments do not correct the defects in cardiac muscle cell numbers and the prognosis of congestive heart failure remains poor. The existence, in adult mammalian heart, of low but detectable cardiomyocyte proliferative capacities has shifted the target of...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 28, 2019 Category: Molecular Biology Source Type: research

What's in a cardiomyocyte – And how do we make one through reprogramming?
Publication date: Available online 25 March 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Benjamin Keepers, Jiandong Liu, Li QianAbstractSubstantial progress is being made in the field cardiac reprogramming, and those in the field are hopeful that the technology will be formulated for therapeutic use. Beyond the excitement around generating a revolutionary new approach for treating ischemic heart diseases, cardiac reprogramming has delivered provocative findings that challenge common notions of cell fate and cell identity. Have we really made de novo cardiomyocytes? To answer this ques...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 25, 2019 Category: Molecular Biology Source Type: research

Dual knockdown of Galectin-8 and its glycosylated ligand, the activated leukocyte cell adhesion molecule (ALCAM/CD166), synergistically delays in vivo breast cancer growth
Publication date: Available online 21 March 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Fátima Ferragut, Alejandro J. Cagnoni, Lucas L. Colombo, Clara Sánchez Terrero, Carlota Wolfenstein-Todel, María F. Troncoso, Silvia I. Vanzulli, Gabriel A. Rabinovich, Karina V. Mariño, María T. ElolaAbstractGalectin-8 (Gal-8), a ‘tandem-repeat’-type galectin, has been described as a modulator of cellular functions including adhesion, spreading, growth arrest, apoptosis, pathogen recognition, autophagy, and immunomodulation. We have previously shown ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 21, 2019 Category: Molecular Biology Source Type: research

Cannabinoid-sensitive receptors in cardiac health and disease
This article is part of a Special Issue entitled: Cardiomyocyte biology: new pathways of differentiation and regeneration edited by Marijke Brinkm, Marcus C. Schaub, and Christian Zuppinger. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 17, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: May 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1866, Issue 5Author(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 17, 2019 Category: Molecular Biology Source Type: research

Oxygen as a key regulator of cardiomyocyte proliferation
This article is part of a Special Issue entitled: Cardiomyocyte biology: new pathways of differentiation and regeneration edited by Marijke Brinkm, Marcus C. Schaub, and Christian Zuppinger. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 16, 2019 Category: Molecular Biology Source Type: research

Role of HDACs in cardiac electropathology: Therapeutic implications for atrial fibrillation
This article is part of a Special Issue entitled: Cardiomyocyte biology: new pathways of differentiation and regeneration edited by Marijke Brinkm, Marcus C. Schaub, and Christian Zuppinger. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 15, 2019 Category: Molecular Biology Source Type: research

Using synthetic peptides and recombinant collagen to understand DDR–collagen interactions
Publication date: Available online 15 March 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Eric A. Chen, Yu-Shan LinAbstractThe discoidin domain receptors, DDR1 and DDR2, are a subfamily of receptor tyrosine kinases that are activated upon binding to collagen. DDR–collagen interactions play an important role in cell proliferation and migration. Over the past few decades, synthetic peptides and recombinant collagen have been developed as tools to study the biophysical characteristics of collagen and various protein–collagen interactions. Herein we review how these techniques ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 15, 2019 Category: Molecular Biology Source Type: research

Lactate dehydrogenase A regulates autophagy and tamoxifen resistance in breast cancer
Publication date: Available online 13 March 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Chandan Kanta Das, Aditya Parekh, Pratap Kumar Parida, Sujit Kumar Bhutia, Mahitosh MandalAbstractEstrogen receptor (ER) antagonist, tamoxifen has been universally used for the treatment of the ER-positive breast cancer; however, inevitable emergence of resistance to tamoxifen obstructs the successful treatment of this cancer. So, there is an immediate requirement for the search of a novel therapeutic target for treatment of this cancer. Acquired tamoxifen resistant breast cancer cell lines MCF-7 ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 15, 2019 Category: Molecular Biology Source Type: research

Anti-metastatic activity of MPT0G211, a novel HDAC6 inhibitor, in human breast cancer cells in vitro and in vivo
In conclusion, our results demonstrate that MPT0G211 reduces TNBC cell motility by promoting cortactin acetylation and aurora-A degradation, and inhibiting the cofilin–F-actin pathway via HDAC6 activity attenuation. MPT0G211 therefore demonstrates therapeutic potential for invasive TNBC.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 11, 2019 Category: Molecular Biology Source Type: research

Long-term self-renewal of naïve neural stem cells in a defined condition
Publication date: Available online 9 March 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Yongming Wu, Xi Chen, Guangjun Xi, Xingliang Zhou, Suyue Pan, Qi-Long YingAbstractDuring embryonic development, neural stem cells (NSCs) emerge as early as the neural plate stage and give rise to the nervous system. Early-stage NSCs express Sry-related-HMG box-1 (Sox1) and are biased towards neuronal differentiation. However, long-term maintenance of early-stage NSCs in vitro remains a challenge. Here, we report development of a defined culture condition for the long-term maintenance of Sox1-positi...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 10, 2019 Category: Molecular Biology Source Type: research

CDK7 is a component of the integrated stress response regulating SNAT2 (SLC38A2)/System A adaptation in response to cellular amino acid deprivation
Publication date: Available online 8 March 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Clare Stretton, Christopher Lipina, Russell Hyde, Emma Cwiklinski, Thorsten M. Hoffmann, Peter M. Taylor, Harinder S. HundalAbstractExtracellular amino acid (AA) withdrawal/restriction invokes an integrated stress response (ISR) that induces global suppression of protein synthesis whilst allowing transcription and translation of a select group of genes, whose protein products facilitate cellular adaptation to AA insufficiency. Transcriptional induction of the System A/SNAT2 AA transporter represent...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 8, 2019 Category: Molecular Biology Source Type: research

Nuclear proteasomal degradation of Saccharomyces cerevisiae inorganic pyrophosphatase Ipp1p, a nucleocytoplasmic protein whose stability depends on its subcellular localization
Publication date: Available online 28 February 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Gloria Serrano-Bueno, Juan Manuel Madroñal, Javier Manzano-López, Manuel Muñiz, José Román Perez-Castiñeira, Agustín Hernández, Aurelio SerranoAbstractInorganic pyrophosphate (PPi) is an abundant by-product of cellular metabolism. PPi-producing reactions take place in the nucleus concurrently with reactions that use PPi as a substrate. Saccharomyces cerevisiae possesses two soluble pyrophosphatases (sPPases): Ipp1p, an essential and allege...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 5, 2019 Category: Molecular Biology Source Type: research

Rat-derived feeder cells immortalized by expression of mutant CDK4, cyclin D, and telomerase can support stem cell growth
In this study, we found that our newly established rat-derived fibroblasts immortalized by the expression of mutant cyclin-dependent kinase 4, cyclin D, and telomerase reverse transcriptase, can function as feeder cells for relatively long cell culture periods of approximately 14 days. The rat-derived immortalized cells developed in this study should be a useful source of feeder cells to support stem cell research.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 5, 2019 Category: Molecular Biology Source Type: research

Autophagy in cardiomyopathies
Publication date: Available online 1 March 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Antonia T.L. Zech, Sonia R. Singh, Saskia Schlossarek, Lucie CarrierAbstractAutophagy (greek auto: self; phagein: eating) is a highly conserved process within eukaryotes that degrades long-lived proteins and organelles within lysosomes. Its accurate and constant operation in basal conditions ensures cellular homeostasis by degrading damaged cellular components and thereby acting not only as a quality control but as well as an energy supplier. An increasing body of evidence indicates a major role of...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - March 5, 2019 Category: Molecular Biology Source Type: research

An element for development: Calcium signaling in mammalian reproduction and development
Publication date: Available online 28 February 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Teneale A. Stewart, Felicity M. DavisAbstractLife begins with calcium. It is the language that a sperm cell uses to respond to instructions from the female reproductive tract to alter its swimming pattern and gain the force required to penetrate the outer layers of the oocyte. The first heartbeat transpires from spontaneous calcium oscillations in embryonic cardiomyocytes. The dynamic balance of calcium between auditory hair cells and the fluid they bathe in enables us to hear our first sound, ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 28, 2019 Category: Molecular Biology Source Type: research

Mechanistic insights into store-operated Ca2+ entry during excitation-contraction coupling in skeletal muscle
This article is part of a Special Issue entitled: ECS Meeting edited by Claus Heizmann, Joachim Krebs and Jacques Haiech. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 27, 2019 Category: Molecular Biology Source Type: research

Mitochondrial presequence import: Multiple regulatory knobs fine-tune mitochondrial biogenesis and homeostasis
Publication date: Available online 22 February 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Cyril Moulin, Anne Caumont-Sarcos, Raffaele IevaAbstractMitochondria are pivotal organelles for cellular signaling and metabolism, and their dysfunction leads to severe cellular stress. About 70% of the mitochondrial proteome consists of preproteins synthesized in the cytosol with an amino-terminal cleavable presequence targeting signal. The TIM23 complex transports presequence signals towards the mitochondrial matrix. Ultimately, the mature protein segments are either transported into the matr...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 23, 2019 Category: Molecular Biology Source Type: research

The vacuolar shapes of ageing: From function to morphology
Publication date: Available online 21 February 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Andreas Aufschnaiter, Sabrina BüttnerAbstractCellular ageing results in accumulating damage to various macromolecules and the progressive decline of organelle function. Yeast vacuoles as well as their counterpart in higher eukaryotes, the lysosomes, emerge as central organelles in lifespan determination. These acidic organelles integrate enzymatic breakdown and recycling of cellular waste with nutrient sensing, storage, signalling and mobilization. Establishing physical contact with virtua...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 22, 2019 Category: Molecular Biology Source Type: research

Pathophysiological characterization of MERRF patient-specific induced neurons generated by direct reprogramming
Publication date: Available online 22 February 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Marina Villanueva-Paz, Suleva Povea-Cabello, Irene Villalón-García, Juan M. Suárez-Rivero, Mónica Álvarez-Córdoba, Mario de la Mata, Marta Talaverón-Rey, Sandra Jackson, José A. Sánchez-AlcázarAbstractMitochondrial diseases are a group of rare heterogeneous genetic disorders caused by total or partial mitochondrial dysfunction. They can be caused by mutations in nuclear or mitochondrial DNA (mtDNA). MERRF (Myoclonic Epilepsy ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 22, 2019 Category: Molecular Biology Source Type: research

Mechanical insights into the regulation of programmed cell death by p53 via mitochondria
Publication date: Available online 18 February 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Kohji Yamada, Kiyotsugu YoshidaAbstractAll organisms end with their death, and many parts of cells die through intrinsic suicide machineries in response to diverse stimuli. These intrinsic cell death pathways are often termed as programmed cell deaths (PCDs), and are critical for organism development, tissue homeostasis and various diseases. Recent evidence has revealed that most of PCDs involve a tumor suppressor p53 and components of the intra-mitochondria. Furthermore, the movement and posit...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 18, 2019 Category: Molecular Biology Source Type: research

DENN domain-containing protein FAM45A regulates the homeostasis of late/multivesicular endosomes
In this study, we characterized cellular roles of FAM45A. We found that FAM45A localized mainly in late/multivesicular endosomes. Depletion of FAM45A resulted in clustering of endosomes to the perinuclear region. The endocytosis of EGF receptor was impaired in FAM45A knockdown cells due to a delay in the early-to-late endosome transition. Furthermore, the secretion of selected exosome subpopulations was also attenuated in FAM45A knockdown cells. Consistent with these results, Rab27a and Rab27b, two Rabs involved in endosome motility and exosome biogenesis, were found to act downstream of FAM45A pathway. FAM45A colocalized ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 14, 2019 Category: Molecular Biology Source Type: research

Lysosomal regulation of extracellular vesicle excretion during d-ribose-induced NLRP3 inflammasome activation in podocytes
In this study, we hypothesized that the NLRP3 inflammasome product, IL-1β in response to exogenously administrated and endogenously produced d-ribose stimulation is released via extracellular vesicles including EVs via a sphingolipid-mediated molecular mechanisms controlling lysosome and multivesicular body (MVB) interaction. First, we demonstrated that both endogenous and exogenous d-ribose induced NLRP3 inflammasome activation to produce IL-1β, which was released via EVs in podocytes. Then, we found that colocalization of marker MVB marker VPS16 with IL-1β within podocytes increased upon d-ribose stimulati...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 14, 2019 Category: Molecular Biology Source Type: research

BAG3 deletion suppresses stem cell-like features of pancreatic ductal adenocarcinoma via translational suppression of ISG15
Publication date: Available online 14 February 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Xin-Yu Li, Jing Yan, Jia Sun, Chao Li, Jing-Yi Jiang, Jia-Mei Wang, Xiao-Na Meng, Jia-Jian Liang, Hua-Qin WangAbstractBAG3 is a member of the cochaperone BAG family and often highly expressed in various cancers. Recently, evidences show that BAG3 promotes stemness of human cancer cells. IFN-stimulated genes 15 (ISG15) is an ubiquitin-like molecule, which is covalently conjugated with substrates to form ISGylated proteins. Global screening BAG3 interacting partners demonstrated that ISG15 might ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 14, 2019 Category: Molecular Biology Source Type: research

PKA negatively modulates the migration enhancing effect of Connexin 43
Publication date: Available online 12 February 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Petra Kameritsch, Felizitas Kiemer, Hanna Mannell, Heike Beck, Ulrich Pohl, Kristin PogodaAbstractConnexin 43 (Cx43) expression is associated with an increased cell migration and related changes of the actin cytoskeleton (enhanced filopodia formation). These effects are mediated by the C-terminal cytoplasmic part of Cx43 in a channel-independent manner. Since this part has been shown to interact with a variety of proteins and has multiple phosphorylation sites we analyzed here a potential role ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 13, 2019 Category: Molecular Biology Source Type: research

5′ processing of Saccharomyces cerevisiae mitochondrial tRNAs requires expression of multiple genes
Publication date: Available online 10 February 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Raquel F. Guedes-Monteiro, Leticia V.R. Franco, Bruno S. Moda, Alexander Tzagoloff, Mário H. BarrosAbstractMitochondrial tRNAs are processed at their 5’ends by highly divergent but ubiquitous RNase P. In Saccharomyces cerevisiae, Rpm2p is the protein component of RNase P. Here, we identify four novel genes MTA1, MTA2, GEP5 and PET130 of the Saccharomycetaceae family that are necessary for an efficient processing of mitochondrial tRNAs. Null mutants of mta1, mta2 and gep5 have sever...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 11, 2019 Category: Molecular Biology Source Type: research

HOTAIR/miR-326/FUT6 axis facilitates colorectal cancer progression through regulating fucosylation of CD44 via PI3K/AKT/mTOR pathway
Publication date: Available online 8 February 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Shimeng Pan, Yanqiu Liu, Qianqian Liu, Yang Xiao, Bing Liu, Xiang Ren, Xia Qi, Huimin Zhou, Changqian Zeng, Li JiaAbstractMetastasis is the main cause of death in colorectal cancer (CRC) patients. Aberrant fucosylation, catalyzed by the specific fucosyltransferases (FUTs), is associated with malignant behaviors. Non-conding RNAs, including long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), emerge as key molecules in cancer malignancy. The aim of this study was to investigate HOTAIR/miR-326/F...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 9, 2019 Category: Molecular Biology Source Type: research

Phosphatidylinositol-3-phosphate-mediated actin domain formation linked to DNA synthesis upon insulin treatment in rat hepatoma-derived H4IIEC3 cells
In this study, we examined the effect of PI3P depletion on the insulin response in rat hepatoma-derived H4IIEC3 cells. We found that insulin treatment induced the transient formation of an actin domain structure, a mesh-like tangled network of actin filaments where phosphorylated Akt, endosomal proteins, and PI3P accumulated. Actin domain formation was repressed by the depletion of PI3P by SAR405, an inhibitor of the class III PI3 kinase, Vps34, by the inhibition of PI3P function by the competitive binding of an excess amount of GST-fused 2xFYVE protein to intracellular PI3P, and by the use of diabetic model cells, in whic...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 9, 2019 Category: Molecular Biology Source Type: research