cAMP regulation of protein phosphatases PP1 and PP2A in brain
Publication date: Available online 18 September 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Shannon Leslie, Angus C. NairnAbstractNormal functioning of the brain is dependent upon a complex web of communication between numerous cell types. Within neuronal networks, the faithful transmission of information between neurons relies on an equally complex organization of inter- and intra-cellular signaling systems that act to modulate protein activity. In particular, post-translational modifications (PTMs) are responsible for regulating protein activity in response to neurochemical signali...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 18, 2018 Category: Molecular Biology Source Type: research

ZIP9 but not the androgen receptor mediates testosterone-induced migratory activity of metastatic prostate cancer cells
Publication date: Available online 15 September 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Ahmed Bulldan, Joerg-Walter Bartsch, Lutz Konrad, Georgios Scheiner-BobisAbstractLNCaP cells are derived from a metastatic lesion of human prostate adenocarcinoma. They express the classical androgen receptor (AR) and ZIP9, a Zn2+ transporter that also binds testosterone and mediates signaling by interacting with G-proteins.Our results show that LNCaP cells respond to testosterone by mobilizing their migratory machinery. Their exposure to testosterone triggers the formation of lamellipodia, re...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 16, 2018 Category: Molecular Biology Source Type: research

Neuroendocrine differentiation contributes to radioresistance development and metastatic potential increase in non-small cell lung cancer
Publication date: Available online 15 September 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Rongying Zhu, Xiaodong Yang, Xiang Xue, Mingjing Shen, Feng Chen, Xiaodong Chen, Ying Tsai, Peter C. Keng, Yongbing Chen, Soo Ok Lee, Yuhchyau ChenAbstractRadiation treatment induces neuroendocrine differentiation (NED) in non-small cell lung cancer (NSCLC) A549 and H157 cells, so higher NE-like features in radioresistant A549 (A549R26-1) and H157 (H157R24-1) cells are observed than in parental cells. We detected higher NED marker expressions in A549R26-1 cell-derived tumors than in A549 cell-...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 15, 2018 Category: Molecular Biology Source Type: research

NRBE3 promotes metastasis of breast cancer by down-regulating E-cadherin expression
Publication date: Available online 11 September 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Tong Zheng, Min Lu, Ting Wang, Chunfeng Zhang, Xiaojuan DuAbstractNRBE3 acts as an E3 ligase of RB to promote RB's polyubiquitination and degradation. In addition, NRBE3 is up-regulated in human breast cancer (BC) tissues. However, how NRBE3 functions in BC is unknown. Here, we show that up-regulation of NRBE3 is correlated with lymphatic metastasis in human BC tissues. Ectopic expression of NRBE3 promotes migration and invasion in BC cells. Accordingly, knockdown of NRBE3 inhibits migration a...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 12, 2018 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: November 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 11, Part AAuthor(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 11, 2018 Category: Molecular Biology Source Type: research

Dual-specificity MAP kinase phosphatases in health and disease
Publication date: Available online 8 September 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Ole-Morten Seternes, Andrew M. Kidger, Stephen M. KeyseAbstractIt is well established that a family of dual-specificity MAP kinase phosphatases (MKPs) play key roles in the regulated dephosphorylation and inactivation of MAP kinase isoforms in mammalian cells and tissues. MKPs provide a mechanism of spatiotemporal feedback control of these key signalling pathways, but can also mediate crosstalk between distinct MAP kinase cascades and facilitate interactions between MAP kinase pathways and othe...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 9, 2018 Category: Molecular Biology Source Type: research

Rasputin a decade on and more promiscuous than ever? A review of G3BPs
Publication date: Available online 5 September 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Umber Alam, Derek KennedyAbstractRas-GTPase-activating protein (SH3 domain)-binding proteins (G3BPs, also known as Rasputin) are a family of RNA binding proteins that regulate gene expression in response to environmental stresses by controlling mRNA stability and translation. G3BPs appear to facilitate this activity through their role in stress granules for which they are considered a core component, however, it should be noted that not all stress granules contain G3BPs and this appears to be c...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 6, 2018 Category: Molecular Biology Source Type: research

Peroxisome development in yeast is associated with the formation of Pex3-dependent peroxisome-vacuole contact sites
Publication date: Available online 1 September 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Huala Wu, Rinse de Boer, Arjen M. Krikken, Arman Akşit, Yuan Wei, Ida J. van der KleiAbstractUsing electron and fluorescence microscopy techniques, we identified various physical contacts between peroxisomes and other cell organelles in the yeast Hansenula polymorpha.In exponential glucose-grown cells, which typically contain a single small peroxisome, contacts were only observed with the endoplasmic reticulum and the plasma membrane. Here we focus on a novel peroxisome-vacuole contact site th...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 2, 2018 Category: Molecular Biology Source Type: research

N-acetylaspartate pathway is nutrient responsive and coordinates lipid and energy metabolism in brown adipocytes
Publication date: Available online 31 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Katharina Huber, Dina C. Hofer, Sophie Trefely, Helmut J. Pelzmann, Corina Madreiter-Sokolowski, Madalina Duta-Mare, Stefanie Schlager, Gert Trausinger, Sarah Stryeck, Wolfgang F. Graier, Dagmar Kolb, Christoph Magnes, Nathaniel W. Snyder, Andreas Prokesch, Dagmar Kratky, Tobias Madl, Kathryn E. Wellen, Juliane G. Bogner-StraussAbstractThe discovery of significant amounts of metabolically active brown adipose tissue (BAT) in adult humans renders it a promising target for anti-obesity therapies by...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 1, 2018 Category: Molecular Biology Source Type: research

Targeting PP2A in cancer: Combination therapies
Publication date: Available online 1 September 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Sahar Mazhar, Sarah E. Taylor, Jaya Sangodkar, Goutham NarlaAbstractThe serine/threonine phosphatase PP2A regulates a vast portion of the phosphoproteome including pathways involved in apoptosis, proliferation and DNA damage response and PP2A inactivation is a vital step in malignant transformation. Many groups have explored the therapeutic venue of combining PP2A reactivation with kinase inhibition to counteract the very changes in tumor suppressors and oncogenes that lead to cancer developmen...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 1, 2018 Category: Molecular Biology Source Type: research

Calcium signaling in health, disease and therapy
Publication date: Available online 30 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Jan B. Parys, Geert Bultynck (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 30, 2018 Category: Molecular Biology Source Type: research

Profiling of signal sequence characteristics and requirement of different translocation components
Publication date: Available online 29 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Chewon Yim, Sung-jun Jung, Ji Eun Hani Kim, Yunjae Jung, Seoyoon Jeong, Hyun KimAbstractThe N-terminal signal sequence (SS) on proteins targeted to the endoplasmic reticulum (ER) is surprisingly diverse in hydrophobicity, in the number of preceding N-terminal residues (N-length), and in charged residues flanking the sequence. However, it remains unclear how these sequences despite their heterogeneity bind to the same site and open the Sec61 translocon. We assessed varying features of SSs and thei...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 29, 2018 Category: Molecular Biology Source Type: research

Far-red acclimating cyanobacterium as versatile source for bright fluorescent biomarkers
We report the generation of chromoproteins, termed BDFPs, that are small, bind auto-catalytically the ubiquitous biliverdin as chromophore, express well, and retain their fluorescence in mammalian cells and in the nematode, C. elegans. They are, moreover, photostable and tolerate high temperature, low pH and chemical denaturation. Homo-bichromophoric tandems of these proteins improve labeling, while hetero-bichromophoric systems with large Stokes shifts are suitable for applications like FRET, multi-channel or super-resolution microscopy. The BDFPs compare favorably to other biliproteins and provide a novel, extremely vers...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 25, 2018 Category: Molecular Biology Source Type: research

Human U3 protein14a is a novel type ubiquitin ligase that binds RB and promotes RB degradation depending on a leucine-rich region
Publication date: Available online 25 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Huijiao Liu, Jiangnan Wang, Yun Liu, Lelin Hu, Chunfeng Zhang, Baocai Xing, Xiaojuan DuAbstractThe nucleolar protein hUTP14a promotes p53 degradation and possesses an oncogene potential. Here, we report that hUTP14a promotes degradation of tumor suppressor retinoblastoma (RB) protein. Sequences alignment showed that hUTP14a contains the RB-binding PENF motif in its C-terminus. We showed that hUTP14a interacted with RB in vivo and in vitro. Further, hUTP14a promoted polyubiquitination and proteaso...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 25, 2018 Category: Molecular Biology Source Type: research

Stimulus-dependent chromatin dynamics, citrullination, calcium signalling and ROS production during NET formation
Publication date: Available online 25 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Cynthia M. de Bont, Werner J.H. Koopman, Wilbert C. Boelens, Ger J.M. PruijnAbstractNeutrophils can release their chromatin to form neutrophil extracellular traps (NETs), a process known as NETosis. Although NET formation can be induced by various stimuli, recent evidence suggests that these stimuli do so via different mechanisms. Here, we have analysed NET formation induced by lipopolysaccharide (LPS), phorbol 12‑myristate 13‑acetate (PMA) and the calcium (Ca2+) ionophore A23187. Our results...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 25, 2018 Category: Molecular Biology Source Type: research

The LIM domain binding protein 1, Ldb1, has distinct roles in Neu-induced mammary tumorigenesis
Publication date: Available online 23 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Sarra Ahmed, Benjamin R. Pryce, Khalid N. Al-Zahrani, Luc A. SabourinAbstractWe have previously shown that the Ste20-like kinase SLK interacts directly with the LIM domain-binding protein 1, Ldb1. Ldb1 knock down in murine fibroblasts activates SLK and enhances cell migration. To investigate the effect of Ldb1 deletion in ErbB2/HER2-driven tumorigenesis, Ldb1 conditional mice were crossed into MMTV-NIC mice, expressing the Neu oncogene and Cre recombinase from a bi-cistronic transgene. Our result...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 24, 2018 Category: Molecular Biology Source Type: research

Anoctamin-6 regulates ADAM sheddase function
We report that overexpression of ANO6 in HEK293T cells led to increased Ca2+-mediated PS-exposure that was indeed accompanied by enhanced release of AREG and TGF-alpha. The effect was not observed when cells were treated with the PKC-dependent ADAM17 activator PMA. Transformation of cells with a constitutively active ANO6 mutant led to spontaneous PS-exposure and to the release of ADAM17-substrates in the absence of any stimuli. Inhibitor experiments indicated that ANO6-mediated enhancement of substrate cleavage simultaneously broadened the spectrum of participating metalloproteinases. In complementary experiments, siRNA-m...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 24, 2018 Category: Molecular Biology Source Type: research

CDNF induces the adaptive unfolded protein response and attenuates endoplasmic reticulum stress-induced cell death
In conclusion, CDNF regulates proteostasis in the ER by inducing the adaptive UPR response and inhibiting apoptotic pathways triggered by ER stress. We propose that neuroprotection induced by CDNF is mediated by regulating ER proteostasis. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 24, 2018 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 10Author(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 23, 2018 Category: Molecular Biology Source Type: research

Serine-threonine protein phosphatases: Lost in translation
Publication date: Available online 20 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Victoria KolupaevaAbstractProtein synthesis is one of the most complex and energy-consuming processes in eukaryotic cells and therefore is tightly regulated. One of the main mechanisms of translational control is post-translational modifications of the components of translational apparatus. Phosphorylation status of translation factors depends on the balanced action of kinases and phosphatases. While many kinase-dependent events are well defined, phosphatases that counteract phosphorylation are r...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 21, 2018 Category: Molecular Biology Source Type: research

Ca2+ signals triggered by bacterial pathogens and microdomains
Publication date: Available online 18 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Guy Tran Van Nhieu, Geneviève Dupont, Laurent CombettesAbstractRecent reports have highlighted the pivotal role of Ca2+ during host cell infection by bacterial pathogens. Here, we review how bacterial pore-forming toxins (PFTs) trigger global Ca2+ signals to regulate cell adhesion-, inflammatory- or death processes. We comment recent reports describing the role of bacterial effectors injected by a type III secretion system (T3SS) as well as host cell players in the formation of Ca2+ microd...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 18, 2018 Category: Molecular Biology Source Type: research

p27Kip1 regulates the microtubule bundling activity of PRC1
Publication date: Available online 16 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Renaud T. Perchey, Murielle P. Serres, Ada Nowosad, Justine Creff, Caroline Callot, Alexandre Gay, Stéphane Manenti, Robert L. Margolis, Anastassia Hatzoglou, Arnaud BessonAbstractCytokinesis begins in anaphase with the formation of the central spindle. PRC1 is a microtubule associated protein that plays an essential role in central spindle formation by crosslinking antiparallel microtubules. We have identified PRC1 as a novel binding partner for p27Kip1 (p27). p27 is a cyclin-CDK inhibito...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 16, 2018 Category: Molecular Biology Source Type: research

Effect of N-acyl-dopamines on beta cell differentiation and wound healing in diabetic mice
Publication date: Available online 16 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Carla Jiménez-Jiménez, Maribel Lara-Chica, Belén Palomares, Juan Antonio Collado, J. Lopez-Miranda, Eduardo Muñoz, Marco A. CalzadoAbstractN-acyl-dopamines are endolipids with neuroprotective, antiinflammatory and immunomodulatory properties. Previously, we showed the ability of these compounds to induce HIF-1α stabilization. Hypoxia and HIF-1α play an important role in the most relevant stages of diabetic pathogenesis. This work analyzes the possible role ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 16, 2018 Category: Molecular Biology Source Type: research

Identification of a splice variant of optineurin which is defective in autophagy and phosphorylation
Publication date: Available online 16 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Shivranjani C. Moharir, Megha Bansal, Gopalakrishna Ramachandran, Rajashree Ramaswamy, Shivali Rawat, Swasti Raychaudhuri, Ghanshyam SwarupAbstractOptineurin (Optn) is an autophagy receptor that performs various functions in cargo-selective and non-selective autophagy. Here, we have identified and characterized a splice variant of mouse optineurin mRNA, which produces a truncated protein lacking N-terminal 157 amino acids (d157mOptn). This mRNA and protein are expressed in several tissues and cel...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 16, 2018 Category: Molecular Biology Source Type: research

Calcium signaling and the lytic cycle of the Apicomplexan parasite Toxoplasma gondii
Publication date: Available online 10 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Miryam Andrea Hortua Triana, Karla M. Márquez-Nogueras, Stephen A. Vella, Silvia N.J. MorenoAbstractToxoplasma gondii has a complex life cycle involving different hosts and is dependent on fast responses, as the parasite reacts to changing environmental conditions. T. gondii causes disease by lysing the host cells that it infects and it does this by reiterating its lytic cycle, which consists of host cell invasion, replication inside the host cell, and egress causing host cell lysis. Calci...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 10, 2018 Category: Molecular Biology Source Type: research

Establishing life is a calcium-dependent TRiP: Transient receptor potential channels in reproduction
Publication date: Available online 9 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Katrien De Clercq, Joris VriensAbstractCalcium plays a key role in many different steps of the reproduction process, from germ cell maturation to placental development. However, the exact function and regulation of calcium throughout subsequent reproductive events remains rather enigmatic. Successful pregnancy requires the establishment of a complex dialogue between the implanting embryo and the endometrium. On the one hand, endometrial cell will undergo massive changes to support an implanting em...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 10, 2018 Category: Molecular Biology Source Type: research

Intracellular calcium dysregulation in autism spectrum disorder: An analysis of converging organelle signaling pathways
Publication date: Available online 9 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Rachel L. Nguyen, Yuliya V. Medvedeva, Tejasvi E. Ayyagari, Galina Schmunk, John Jay GargusAbstractAutism spectrum disorder (ASD) is a group of complex, neurological disorders that affect early cognitive, social, and verbal development. Our understanding of ASD has vastly improved with advances in genomic sequencing technology and genetic models that have identified>800 variants that increase susceptibility to ASD. Although these findings have confirmed its high heritability, the underlying mec...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 10, 2018 Category: Molecular Biology Source Type: research

Suppression of PTBP1 signaling is responsible for mesenchymal stem cell induced invasion of low malignancy cancer cells
Publication date: Available online 9 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Xin Fu, Fangnan Xie, Fuxing Gong, Zhigang Yang, Xiaoyan Lv, Xintian Li, Hu Jiao, Qian Wang, Xia Liu, Li Yan, Ran XiaoAbstractMesenchymal stem cells (MSCs) hold great promise as attractive vehicles to deliver therapeutic agents against cancer, while the cross-talk between MSCs and cancer cells remains controversial. Here in an indirect co-culture system we observed that MSCs induced the malignancy transformation of low malignancy cancer cells HT29 and MCF7, whereas MSCs were reprogrammed by high ma...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 10, 2018 Category: Molecular Biology Source Type: research

RBM4a modulates the impact of PRDM16 on development of brown adipocytes through an alternative splicing mechanism
In this study, results of transcriptome analyses revealed the reprogrammed splicing profile of the PR domain containing 16 (PRDM16) gene, a key transcription factor involved in brown adipogenesis, throughout development of wild-type brown adipose tissues (BATs). Moreover, discriminative splicing patterns of PRDM16 transcripts were noted in embryonic and postnatal RBM4a−/− BATs. Overexpression of RBM4a enhanced the relative levels of PRDM16-ex 16 transcripts by simultaneously interacting with exonic and intronic CU elements, which encoded the PRDM16S isoform containing a distinct C-terminus. The presence of the ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 8, 2018 Category: Molecular Biology Source Type: research

Live-cell imaging of early coat protein dynamics during clathrin-mediated endocytosis
Publication date: Available online 3 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Masashi Miyashita, Ryutaro Kashikuma, Makoto Nagano, Junko Y. Toshima, Jiro ToshimaAbstractClathrin-mediated endocytosis is an essential process that is mediated by the stepwise appearance or disappearance of many different proteins at the plasma membrane. In the budding yeast, these proteins are categorized into at least five modules, according to their spatiotemporal dynamics. Among them, the dynamics of proteins in the late coat module are well characterized, but those in the early coat module ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 3, 2018 Category: Molecular Biology Source Type: research

The role of pseudophosphatases as signaling regulators
Publication date: Available online 2 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Shantá D. HintonAbstractPseudophosphatases are atypical members of the protein tyrosine phosphatase superfamily. Mutations within their catalytic signature motif render them catalytically inactive. Despite this lack of catalytic function, pseudophosphatases have been implicated in various diseases such as Charcot Marie-Tooth disorder, cancer, metabolic disorder, and obesity. Moreover, they have roles in various signaling networks such as spermatogenesis, apoptosis, stress response, tumorige...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 2, 2018 Category: Molecular Biology Source Type: research

Myosin phosphatase: Unexpected functions of a long-known enzyme
This article is part of a Special Issue entitled: Protein Phosphatases as Critical Regulators for Cellular Homeostasis edited by Prof. Peter Ruvolo and Dr. Veerle Janssens. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 2, 2018 Category: Molecular Biology Source Type: research

Ubiquitin protease Ubp8 is necessary for S. cerevisiae respiration
Publication date: Available online 2 August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Manuela Leo, Giulia Fanelli, Serena Di Vito, Barbara Traversetti, Mariafrancesca La Greca, Raffaele A. Palladino, Arianna Montanari, Silvia Francisci, Patrizia FileticiAbstractHealthy mitochondria are required in cell metabolism and deregulation of underlying mechanisms is often involved in human diseases and neurological disorders. Post-translational modifications of mitochondrial proteins regulate their function and activity, accordingly, impairment of ubiquitin proteasome system affects mitocho...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 2, 2018 Category: Molecular Biology Source Type: research

Calcium, a pivotal player in photodynamic therapy?
This article is part of a Special Issue entitled: Calcium signaling in health, disease and therapy edited by Geert Bultynck and Jan Parys. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - August 1, 2018 Category: Molecular Biology Source Type: research

Novel nuclear translocation of inositol polyphosphate 4-phosphatase is associated with cell cycle, proliferation and survival
In this study, we demonstrate for the first time that INPP4A shuttles between the cytoplasm and the nucleus. Nuclear INPP4A is enzymatically active and in dynamic equilibrium between the nucleus and cytoplasm depending on the cell cycle stage, with highest amounts detected in the nucleus during the G0/G1 phase. Moreover, nuclear INPP4A is found to have direct proliferation suppressive activity. Cells constitutively overexpressing nuclear INPP4A exhibit massive apoptosis. In human tissues as well as cell lines, lower nuclear localization of INPP4A correlate with cancerous growth. Together, our findings suggest that nuclear ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 31, 2018 Category: Molecular Biology Source Type: research

Calcium signaling in Alzheimer's disease & therapies
Publication date: Available online 29 July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Benjamin Chun-Kit Tong, Aston Jiaxi Wu, Min Li, King-Ho CheungAbstractAlzheimer's disease (AD) is the most common type of dementia and is characterized by the accumulation of amyloid (Aβ) plaques and neurofibrillary tangles in the brain. Much attention has been given to develop AD treatments based on the amyloid cascade hypothesis; however, none of these drugs had good efficacy at improving cognitive functions in AD patients suggesting that Aβ might not be the disease origin. Thus, there ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 29, 2018 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: September 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 9Author(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 27, 2018 Category: Molecular Biology Source Type: research

Functions and therapeutic potential of protein phosphatase 1: Insights from mouse genetics
Publication date: Available online 26 July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Monica Ferreira, Monique Beullens, Mathieu Bollen, Aleyde Van EyndeAbstractProtein phosphatase 1 (PP1) catalyzes more than half of all phosphoserine/threonine dephosphorylation reactions in mammalian cells. In vivo PP1 does not exist as a free catalytic subunit but is always associated with at least one regulatory PP1-interacting protein (PIP) to generate a large set of distinct holoenzymes. Each PP1 complex controls the dephosphorylation of only a small subset of PP1 substrates. We screened the li...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 27, 2018 Category: Molecular Biology Source Type: research

Targeting Bcl-2-IP3 receptor interaction to treat cancer: A novel approach inspired by nearly a century treating cancer with adrenal corticosteroid hormones
Publication date: Available online 25 July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Clark W. DistelhorstAbstractBcl-2 inhibits cell death by at least two different mechanisms. On the one hand, its BH3 domain binds to pro-apoptotic proteins such as Bim and prevents apoptosis induction. On the other hand, the BH4 domain of Bcl-2 binds to the inositol 1,4,5-trisphosphate receptor (IP3R), preventing Ca2+ signals that mediate cell death. In normal T-cells, Bcl-2 levels increase during the immune response, protecting against cell death, and then decline as apoptosis ensues and the immun...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 25, 2018 Category: Molecular Biology Source Type: research

Secretion and function of Cln5 during the early stages of Dictyostelium development
In this study, we generated an antibody against Cln5 to show that the endogenous protein is secreted during the early stages of Dictyostelium development. Like human CLN5, the Dictyostelium homolog is glycosylated and requires this post-translational modification for secretion. Cln5 secretion bypasses the Golgi complex, and instead, occurs via an unconventional pathway linked to autophagy. Interestingly, we observed co-localization of Cln5 and GFP-Cln3 as well as increased secretion of Cln5 and Cln5-GFP in cln3− cells. Loss of Cln5 causes defects in adhesion and chemotaxis, which intriguingly, has also been reported ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 23, 2018 Category: Molecular Biology Source Type: research

Ryanodine receptor dysfunction in human disorders
Publication date: Available online 21 July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Alexander Kushnir, Benjamin Wajsberg, Andrew R. MarksAbstractRegulation of intracellular calcium (Ca2+) is critical in all cell types. The ryanodine receptor (RyR), an intracellular Ca2+ release channel located on the sarco/endoplasmic reticulum (SR/ER), releases Ca2+ from intracellular stores to activate critical functions including muscle contraction and neurotransmitter release. Dysfunctional RyR-mediated Ca2+ handling has been implicated in the pathogenesis of inherited and non-inherited condit...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 21, 2018 Category: Molecular Biology Source Type: research

The multiple functions of protein phosphatase 6
Publication date: Available online 21 July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Takashi OhamaAbstractThis review presents the accumulating evidence for the roles of protein phosphatase 6 (PP6) in cell cycle, DNA damage repair, inflammatory signaling, lymphocyte development, virus infection, tumor formation/progression, cell/tissue size, and non-coding RNA-mediated regulation. PP6 is an evolutionarily conserved and ubiquitously expressed Ser/Thr protein phosphatase most closely related to protein phosphatase 2A (PP2A) and protein phosphatase 4 (PP4). Although abundantly express...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 21, 2018 Category: Molecular Biology Source Type: research

Protein phosphatases in chromatin structure and function
Publication date: Available online 20 July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Raquel Sales Gil, Paola VagnarelliAbstractChromatin structure and dynamics are highly controlled and regulated processes that play an essential role in many aspects of cell biology. The chromatin transition stages and the factors that control this process are regulated by post-translation modifications, including phosphorylation. While the role of protein kinases in chromatin dynamics has been quite well studied, the nature and regulation of the counteracting phosphatases represent an emerging fiel...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 21, 2018 Category: Molecular Biology Source Type: research

Long non-coding RNA PVT1 promotes malignancy in human endometrial carcinoma cells through negative regulation of miR-195-5p
Publication date: Available online 19 July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Fanfei Kong, Jian Ma, Hui Yang, Di Yang, Cuicui Wang, Xiaoxin MaAbstractThe plasmacytoma variant translocation 1 (PVT1)1 gene is a long non-coding RNA (lncRNA)2 that has been shown to be an oncogene in many cancers. Herein, the function and potential molecular mechanisms connecting PVT1 and miR-195-5p were elucidated in endometrial cancer cell lines. Quantitative real-time PCR and fluorescence in situ hybridization (FISH)3 demonstrated that PVT1 is up-regulated concomitant with miR-195-5p down-regu...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 20, 2018 Category: Molecular Biology Source Type: research

Interaction of arsenic with gap junction protein connexin 43 alters gap junctional intercellular communication
Publication date: Available online 19 July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Afaq Hussain, Subhajit Das Sarma, Swathy Babu, Debnath Pal, Jayasri Das SarmaAbstractChronic exposure to Arsenic pollution in ground water is one of the largest environmental health disasters in the world. The toxicity of trivalent Arsenicals primarily happens due to its interaction with sulfhydryl groups in proteins. Arsenic binding to the protein can change the conformation of the protein and alter its interactions with other proteins leading to tissue damage. Therefore, much importance has been ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 20, 2018 Category: Molecular Biology Source Type: research

Degradation of fibrin-β amyloid co-aggregate: A novel function attributed to ubiquitin
Publication date: Available online 19 July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Payel Bhattacharjee, Debashree De, Debasish BhattacharyyaAbstractHuman placental extract contains numerous bioactive components and are effective wound healing, antimicrobial and anti-inflammatory agents. During our investigation on the therapeutic potency of human placental extract, we have purified ubiquitin like molecules that showed strong fibrino(geno)lytic activity. Further investigation confirmed similar potency of ubiquitin purified from adult human erythrocyte. Additionally, ubiquitin effi...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 19, 2018 Category: Molecular Biology Source Type: research

Do metabolic HAD phosphatases moonlight as protein phosphatases?
Publication date: Available online 18 July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Antje GohlaAbstractMammalian haloacid dehalogenase (HAD)-type phosphatases have evolved to dephosphorylate a wide range of small metabolites, but can also target macromolecules such as serine/threonine, tyrosine-, and histidine-phosphorylated proteins. To accomplish these tasks, HAD phosphatases are equipped with cap domains that control access to the active site and provide substrate specificity determinants. A number of capped HAD phosphatases impact protein phosphorylation, although structural d...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 18, 2018 Category: Molecular Biology Source Type: research

Physiologic functions of PP2A: Lessons from genetically modified mice
Publication date: Available online 18 July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Sara Reynhout, Veerle JanssensAbstractProtein Phosphatase 2A (PP2A) encompasses a large family of Ser/Thr phosphatases, consisting of a catalytic C subunit and a structural A subunit that are, in most cases, further bound to a regulatory B-type subunit. The B-type subunits determine function and regulation of PP2A trimers, but despite their importance in PP2A biology, their roles in controlling dephosphorylation of a given substrate in a given cell or tissue remain poorly defined, particularly in t...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 18, 2018 Category: Molecular Biology Source Type: research

Role of protein phosphatases in the cancer microenvironment
Publication date: Available online 17 July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Peter P. RuvoloAbstractCancer cells depend on a supportive niche (the tumor microenvironment) that promotes tumor cell survival while protecting the malignant cells from therapeutic challenges and the host's defense systems. Cancer cells and the support cells in the tumor microenvironment communicate via cytokines/chemokines, cell:cell contact, or alterations in the metabolic state of the niche (e.g. hypoxia) that promote growth and survival of the tumor cell, influence metastasis, and defeat immun...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 18, 2018 Category: Molecular Biology Source Type: research

The roles of protein tyrosine phosphatases in bone-resorbing osteoclasts
Publication date: Available online 17 July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Moran Shalev, Ari ElsonAbstractMaintaining the proper balance between osteoblast-mediated production of bone and its degradation by osteoclasts is essential for health. Osteoclasts are giant phagocytic cells that are formed by fusion of monocyte-macrophage precursor cells; mature osteoclasts adhere to bone tightly and secrete protons and proteases that degrade its matrix. Phosphorylation of tyrosine residues in proteins, which is regulated by the biochemically-antagonistic activities of protein tyr...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 18, 2018 Category: Molecular Biology Source Type: research