STAC proteins: The missing link in skeletal muscle EC coupling and new regulators of calcium channel function
Publication date: Available online 10 December 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Bernhard E. Flucher, Marta CampiglioAbstractExcitation-contraction coupling is the signaling process by which action potentials control calcium release and consequently the force of muscle contraction. Until recently, three triad proteins were known to be essential for skeletal muscle EC coupling: the voltage-gated calcium channel CaV1.1 acting as voltage sensor, the SR calcium release channel RyR1 representing the only relevant calcium source, and the auxiliary CaV β1a subunit. Whether Ca...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 10, 2018 Category: Molecular Biology Source Type: research

Nuclear RXRα and RXRβ receptors exert distinct and opposite effects on RA-mediated neuroblastoma differentiation
Publication date: Available online 7 December 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Carolina Saibro Girardi, Diana Carolina Rostirolla, Fernanda Janini Mota Lini, Pedro Ozorio Brum, Jeferson Delgado, Camila Tiefensee Ribeiro, Alexsander Alves Teixeira, Daniel Oppermann Peixoto, Luana Heimfarth, Alice Kunzler, José Cláudio Fonseca Moreira, Daniel Pens GelainAbstractRetinoic acid (RA) promotes differentiation in multiple neurogenic cell types by promoting gene reprogramming through retinoid receptors and also by inducing cytosolic signaling events. The nuclear RXR r...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 7, 2018 Category: Molecular Biology Source Type: research

Stress is an agonist for the induction of programmed cell death: A review
Publication date: Available online 5 December 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): David R. Zhou, Rawan Eid, Eric Boucher, Katie A. Miller, Craig A. Mandato, Michael T. GreenwoodAbstractThe prevailing models of stress induced Programmed Cell Death (PCD) posit that excess extracellular chemicals interact with or enter cells and disrupts cellular homeostasis. This activates signalling cascades involving the mitochondria, an increase in the steady state levels of Reactive Oxygen Species (ROS) as well as the activation of Bax and caspases. Further, the increased ROS also causes ce...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 6, 2018 Category: Molecular Biology Source Type: research

Activation of PERK-eIF2α-ATF4-CHOP axis triggered by excessive ER stress contributes to Pb-induced nephrotoxicity
In this study, data showed that Pb could induce ER stress as shown by increased phosphorylation of PERK with subsequent activation of the eIF2α-ATF4-CHOP axis in primary rat proximal tubular (rPT) cells, indicating the activation of PERK-eIF2α-ATF4-CHOP pathway due to excessive ER stress. Pb-activated PERK pathway can be effectively inhibited by 4-phenylbutyric acid and PERK gene silencing, respectively; whereas continuously up-regulated by tunicamycin (TM) treatment. Moreover, Pb-induced apoptosis and inhibition of autophagic flux in rPT cells were significantly augmented and aggravated by co-treatment with TM...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 5, 2018 Category: Molecular Biology Source Type: research

Endoplasmic reticulum stress enhances the rate of endocytosis in calreticulin deficient cells
Publication date: Available online 5 December 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Hamid Massaeli, Divya Viswanathan, Dhanya Govind Pillai, Nasrin MesaeliAbstractCalreticulin an endoplasmic reticulum (ER) chaperone that is involved in the quality control process and plays an important role as a regulator of intracellular calcium homeostasis. Previously, we illustrated that loss of calreticulin function (crt−/−) results in the activation of ubiquitin-proteasome pathway facilitating the increased resistance to apoptosis. Our preliminary data illustrated a significant...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 5, 2018 Category: Molecular Biology Source Type: research

Tuning store-operated calcium entry to modulate Ca2+-dependent physiological processes
Publication date: Available online 3 December 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Hwei Ling Ong, Krishna Prasad Subedi, Ga-Yeon Son, Xibao Liu, Indu Suresh AmbudkarAbstractThe intracellular calcium signaling processes are tightly regulated to ensure the generation of calcium signals with the specific spatiotemporal characteristics that are required for regulating various cell functions. Compartmentalization of the molecular components involved in generating these signals at discrete intracellular sites ensures the signaling specificity and transduction fidelity of the signal ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 4, 2018 Category: Molecular Biology Source Type: research

Dual functions of STAT3 in LPS-induced angiogenesis of hepatocellular carcinoma
In this study, we explored the significant dual functions of active signal transducer and activator of transcription 3 (STAT3) in LPS-induced angiogenesis of HCC. The in vitro effects of active STAT3 in tumor cells and endothelial cells were assessed using angiogenesis assay, ELISA, confocal assay, flow cytometry and western blot. The in vivo role of active STAT3 was assessed in xenografts model in nude mice. Here we report a novel mechanism by which LPS/STAT3 signaling promotes the angiogenesis of HCC both in vitro and in vivo. STAT3 activated by LPS increases the production of vascular endothelial growth factor (VEGF) by...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 4, 2018 Category: Molecular Biology Source Type: research

5-Azido-8-ethynyl-NAADP: A bifunctional, clickable photoaffinity probe for the identification of NAADP receptors
Publication date: Available online 4 December 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Gihan S. Gunaratne, Peiling Su, Jonathan S. Marchant, James T. Slama, Timothy F. WalsethAbstractNicotinic acid adenine dinucleotide phosphate is an evolutionarily conserved second messenger, which mobilizes Ca2+ from acidic stores. The molecular identity of the NAADP receptor has yet to be defined. In pursuit of isolating and identifying NAADP-binding proteins, we synthesized and characterized a bifunctional probe that incorporates both a photoactivatable crosslinking azido moiety at the 5-posit...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 4, 2018 Category: Molecular Biology Source Type: research

Biased perspectives on formyl peptide receptors
Publication date: Available online 4 December 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Carsten Alexander Raabe, Jieny Gröper, Ursula RescherAbstractThe innate immune system is the first line of defense against pathogenic threats. For the early pathogen recognition and activation of cell protective mechanisms, germline-encoded pattern recognition receptors (PRRs) detect characteristic and evolutionary conserved pathogen-associated molecular patterns (PAMPs). PRRs are therefore key elements in the innate immune response; in addition, they sense danger-associated molecular patte...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 4, 2018 Category: Molecular Biology Source Type: research

Applications of FLIKA, a Python-based image processing and analysis platform, for studying local events of cellular calcium signaling
This article is part of a Special Issue entitled: ECS Meeting edited by Claus Heizmann, Joachim Krebs and Jacques Haiech. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 28, 2018 Category: Molecular Biology Source Type: research

Regulation of plasticity and biological features of endothelial progenitor cells by MSC-derived SDF-1
In conclusions, MSC-derived SDF-1 niche plays an important role in the development of EPCs and this niche is essential for bone marrow repopulation by these cells and can enhance the efficiency of EPC therapy for ischemic diseases.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 28, 2018 Category: Molecular Biology Source Type: research

NAD binding by human CD38 analyzed by Trp189 fluorescence
This article is part of a Special Issue entitled: ECS Meeting edited by Claus Heizmann, Joachim Krebs and Jacques Haiech. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 23, 2018 Category: Molecular Biology Source Type: research

Bright near-infrared fluorescence bio-labeling with a biliprotein triad
We report a fluorescent biliprotein triad, termed BDFP1.1:3.1:1.1, that combines a large red-shift (722 nm) with high brightness in mammalian cells and high stability under changing environmental conditions. It is fused from derivatives of the phycobilisome core subunits, ApcE2 and ApcF2. These two subunits are induced by far-red light (FR, 650–700 nm) in FR acclimated cyanobacteria. Two BDFP1.1 domains engineered from ApcF2 covalently bind biliverdin that is accessible in most cells. The soluble BDFP3 domain, engineered from ApcE2, binds phytochromobilin non-covalently, generating BDFP3.1. This phytochromobilin ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 22, 2018 Category: Molecular Biology Source Type: research

Actin dynamics during Ca2+-dependent exocytosis of endothelial Weibel-Palade bodies
This article is part of a Special Issue entitled: ECS Meeting edited by Claus Heizmann, Joachim Krebs and Jacques Haiech. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 21, 2018 Category: Molecular Biology Source Type: research

Transglutaminase-mediated cross-linking of WDR54 regulates EGF receptor-signaling
Publication date: Available online 17 November 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Akane Maeda, Tasuku Nishino, Ryota Matsuzaki, Atsushi Yokoyama, Hiroshi Suga, Toshiki Yagi, Hiroaki KonishiAbstractWDR54 is a member of the WD40 repeat (WDR) domain-containing protein family that was recently identified as a novel oncogene in colorectal cancer. However, the molecular mechanism of WDR54 and its functional association with other molecules related to tumor cell growth are unknown. Here, we show that WDR54 can be cross-linked by the action of transglutaminase (TG) 2, which enhances...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 17, 2018 Category: Molecular Biology Source Type: research

Store-operated Ca2+ entry and Ca2+ responses to hypothalamic releasing hormones in anterior pituitary cells from Orai1−/− and heptaTRPC knockout mice
Publication date: Available online 17 November 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Lucía Núñez, Gary S. Bird, Elena Hernando-Pérez, Enrique Pérez-Riesgo, James W. Putney, Carlos VillalobosAbstractStore operated Ca2+ entry (SOCE) is the most important Ca2+ entry pathway in non-excitable cells. However, SOCE can also play a pivotal role in excitable cells such as anterior pituitary (AP) cells. The AP gland contains five different cell types that release six major AP hormones controlling most of the entire endocrine system. AP hormone release i...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 17, 2018 Category: Molecular Biology Source Type: research

IP3 receptors and Ca2+ entry
Publication date: Available online 15 November 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Nagendra Babu Thillaiappan, Pragnya Chakraborty, Gaiti Hasan, Colin W. TaylorAbstractInositol 1,4,5-trisphosphate receptors (IP3R) are the most widely expressed intracellular Ca2+ release channels. Their activation by IP3 and Ca2+ allows Ca2+ to pass rapidly from the ER lumen to the cytosol. The resulting increase in cytosolic [Ca2+] may directly regulate cytosolic effectors or fuel Ca2+ uptake by other organelles, while the decrease in ER luminal [Ca2+] stimulates store-operated Ca2+ entry (SO...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 16, 2018 Category: Molecular Biology Source Type: research

Depletion of thiol reducing capacity impairs cytosolic but not mitochondrial iron-sulfur protein assembly machineries
Publication date: February 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1866, Issue 2Author(s): Joseph J. Braymer, Martin Stümpfig, Stefanie Thelen, Ulrich Mühlenhoff, Roland LillAbstractIron‑sulfur (Fe/S) clusters are versatile inorganic cofactors that play central roles in essential cellular functions, from respiration to genome stability.>30 proteins involved in Fe/S protein biogenesis in eukaryotes are known, many of which bind clusters via cysteine residues. This opens up the possibility that the thiol-reducing glutaredoxin and thioredoxin systems are required at bot...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 16, 2018 Category: Molecular Biology Source Type: research

Trafficking of the amino acid transporter B0,+ (SLC6A14) to the plasma membrane involves an exclusive interaction with SEC24C for its exit from the endoplasmic reticulum
This study focused on the possible role of SEC24 proteins in ATB0,+ trafficking. Rat ATB0,+ was expressed in HEK293 cells, its localization and trafficking were examined by Western blot, deglycosylation, immunofluorescence (co-localization with ER and trans-Golgi markers) and biotinylation. The expression of ATB0,+ at the plasma membrane was decreased by dominant negative mutants of SAR1, a GTPase, whose activity triggers the formation of the COPII complex. ATB0,+ co-precipitated with SEC24C (but not with the remaining isoforms A, B and D). This interaction was confirmed by immunocytochemistry and the proximity ligation as...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 14, 2018 Category: Molecular Biology Source Type: research

Depletion of thiol reducing capacity impairs cytosolic but not mitochondrial Fe/S protein assembly machineries
Publication date: Available online 10 November 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Joseph J. Braymer, Martin Stümpfig, Stefanie Thelen, Ulrich Mühlenhoff, Roland LillAbstractIron‑sulfur (Fe/S) clusters are versatile inorganic cofactors that play central roles in essential cellular functions, from respiration to genome stability.>30 proteins involved in Fe/S protein biogenesis in eukaryotes are known, many of which bind clusters via cysteine residues. This opens up the possibility that the thiol-reducing glutaredoxin and thioredoxin systems are required at both ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 11, 2018 Category: Molecular Biology Source Type: research

The novel exchange protein activated by cyclic AMP 1 (EPAC1) agonist, I942, regulates inflammatory gene expression in human umbilical vascular endothelial cells (HUVECs)
Publication date: Available online 9 November 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Jolanta Wiejak, Boy van Basten, Urszula Luchowska-Stańska, Graham Hamilton, Stephen J. YarwoodAbstractExchange protein activated by cyclic AMP (EPAC1) suppresses multiple inflammatory actions in vascular endothelial cells (VECs), partly due to its ability to induce expression of the suppressor of cytokine signalling 3 (SOCS3) gene, the protein product of which inhibits interleukin 6 (IL6) signalling through the JAK/STAT3 pathway. Here, for the first time, we use the non-cyclic nucleotide EPAC1 ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 9, 2018 Category: Molecular Biology Source Type: research

Mining of Ebola virus entry inhibitors identifies approved drugs as two-pore channel pore blockers
This article is part of a Special Issue entitled: ECS Meeting edited by Claus Heizmann, Joachim Krebs and Jacques Haiech. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 6, 2018 Category: Molecular Biology Source Type: research

STIM1 and Orai1 regulate Ca2+ microdomains for activation of transcription
We describe the principles of spatiotemporal activation of the transcription factor NFAT and compare its signaling characteristics to those of the autophagy regulating transcription factors, MITF and TFEB. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 6, 2018 Category: Molecular Biology Source Type: research

The deubiquitination of the PTS1-import receptor Pex5p is required for peroxisomal matrix protein import
In this study, we have analyzed a Pex5p-truncation lacking Cys6 [(Δ6)Pex5p], a construct with a ubiquitin-moiety genetically fused to the truncation [Ub-(Δ6)Pex5p], as well as a construct with a reduced susceptibility to deubiquitination [Ub(G75/76A)-(Δ6)Pex5p]. While the (Δ6)Pex5p-truncation is not functional, the Ub-(Δ6)Pex5p chimeric protein can facilitate matrix protein import. In contrast, the Ub(G75/76A)-(Δ6)Pex5p chimera exhibits a complete PTS1-import defect. The data show for the first time that not only ubiquitination but also deubiquitination rates are tightly regulated and th...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 6, 2018 Category: Molecular Biology Source Type: research

Phosphorylation of ELAVL1 (Ser219/Ser316) mediated by PKC is required for erythropoiesis
In conclusion, our study reveals that PKC-mediated phosphorylation of elavl1a at Ser219 and Ser316 sites controls its nucleo-cytoplasmic translocation in zebrafish, thereby regulating embryonic erythropoiesis.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 3, 2018 Category: Molecular Biology Source Type: research

The protein interaction networks of mucolipins and two-pore channels
This article is part of a Special Issue entitled: ECS Meeting edited by Claus Heizmann, Joachim Krebs and Jacques Haiech.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 3, 2018 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: January 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1866, Issue 1Author(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 3, 2018 Category: Molecular Biology Source Type: research

Reviewer Acknowledgement
Publication date: January 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1866, Issue 1Author(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 3, 2018 Category: Molecular Biology Source Type: research

Protein Phosphatases as Critical Regulators for Cellular Homeostasis
Publication date: January 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1866, Issue 1Author(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 3, 2018 Category: Molecular Biology Source Type: research

Development of mKO2 fusion proteins for real-time imaging and mechanistic investigation of the degradation kinetics of human IκBα in living cells
Publication date: Available online 31 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Nilufar Rahimova, Hasan Babazada, Yuriko Higuchi, Fumiyoshi Yamashita, Mitsuru HashidaAbstractIn resting cells, the nuclear factor kappa B (NF-κB) family of transcription factors is stabilized by complexation with the cytoplasmic inhibitor of kappa B alpha (IκBα). Extracellular stimuli, such as tumor necrosis factor alpha (TNFα) or bacterial lipopolysaccharide activate NF-κB through IκBα phosphorylation and ubiquitin-proteasomal degradation. Herein, we d...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 1, 2018 Category: Molecular Biology Source Type: research

Isoform-specific domain organization determines conformation and function of the peroxisomal biogenesis factor PEX26
Publication date: Available online 23 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Philipp Guder, Amelie S. Lotz-Havla, Mathias Woidy, Dunja D. Reiß, Marta K. Danecka, Ulrich A. Schatz, Marc Becker, Regina Ensenauer, Philipp Pagel, Lars Büttner, Ania C. Muntau, Søren W. GerstingAbstractPeroxisomal biogenesis factor PEX26 is a membrane anchor for the multi-subunit PEX1-PEX6 protein complex that controls ubiquitination and dislocation of PEX5 cargo receptors for peroxisomal matrix protein import. PEX26 associates with the peroxisomal translocation pore via PEX1...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 31, 2018 Category: Molecular Biology Source Type: research

Neurite atrophy and apoptosis mediated by PERK signaling after accumulation of GM2-ganglioside
Publication date: Available online 30 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): María José Virgolini, Constanza Feliziani, María Julia Cambiasso, Pablo H. Lopez, Mariana BolloAbstractGM2-gangliosidosis, a subgroup of lysosomal storage disorders, is caused by deficiency of hexosaminidase activity, and comprises the closely related Tay-Sachs and Sandhoff diseases. The enzyme deficiency prevents normal metabolization of ganglioside GM2, usually resulting in progressive neurodegenerative disease.The molecular mechanisms whereby GM2 accumulation in neurons t...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 30, 2018 Category: Molecular Biology Source Type: research

Inhibition of PIM1 blocks the autophagic flux to sensitize glioblastoma cells to ABT-737-induced apoptosis
Publication date: Available online 30 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): J. Remy, B. Linder, U. Weirauch, J. Konovalova, R. Marschalek, A. Aigner, D. KögelAbstractOvercoming apoptosis resistance is one major issue in glioblastoma (GB) therapies. Accumulating evidence indicates that resistance to apoptosis in GB is mediated via upregulation of pro-survival BCL2-family members. The synthetic BH3-mimetic ABT-737 effectively targets BCL2, BCL2 like 1 and BCL2 like 2 but still barely affects cell survival which is presumably due to its inability to inhibit myeloid ce...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 30, 2018 Category: Molecular Biology Source Type: research

S100 proteins: Diagnostic and prognostic biomarkers in laboratory medicine
Publication date: Available online 28 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Claus W. HeizmannAbstractS100 proteins are members of the superfamily of Ca2+-binding proteins characterized by the specific Ca2+-binding motif, the EF-hand. Proteins of this superfamily are of clinical use as important diagnostic and prognostic biomarkers in adult and pediatric Laboratory Medicine. For example, measurements of troponin are nowadays the ‘gold standard’ in the diagnosis of patients with acute coronary syndrome. Parvalbumins were identified as major fish allergens and ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 29, 2018 Category: Molecular Biology Source Type: research

Calcium, mitochondria and cell metabolism: A functional triangle in bioenergetics
Publication date: Available online 26 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Alice Rossi, Paola Pizzo, Riccardo FiladiAbstractThe versatility of mitochondrial metabolism and its fine adjustments to specific physiological or pathological conditions regulate fundamental cell pathways, ranging from proliferation to apoptosis. In particular, Ca2+ signalling has emerged as a key player exploited by mitochondria to tune their activity according with cell demand. The functional interaction between mitochondria and endoplasmic reticulum (ER) deeply impacts on the correct mitocho...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 27, 2018 Category: Molecular Biology Source Type: research

Isoform-specific domain-organization determines conformation and function of the peroxisomal biogenesis factor PEX26
Publication date: Available online 23 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Philipp Guder, Amelie S. Lotz-Havla, Mathias Woidy, Dunja D. Reiß, Marta K. Danecka, Ulrich A. Schatz, Marc Becker, Regina Ensenauer, Philipp Pagel, Lars Büttner, Ania C. Muntau, Søren W. GerstingAbstractPeroxisomal biogenesis factor PEX26 is a membrane anchor for the multi-subunit PEX1-PEX6 protein complex that controls ubiquitination and dislocation of PEX5 cargo receptors for peroxisomal matrix protein import. PEX26 associates with the peroxisomal translocation pore via PEX1...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 23, 2018 Category: Molecular Biology Source Type: research

Epidermal Growth Factor – Based adhesion substrates elicit myoblast scattering, proliferation, differentiation and promote satellite cell myogenic activation
Publication date: Available online 18 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Paola D'Andrea, Marina Sciancalepore, Katerina Veltruska, Paola Lorenzon, Antonella BandieraAbstractThe biochemical properties of muscle extracellular matrix are essential for stem cell adhesion, motility, proliferation and myogenic development. Recombinant elastin-like polypeptides are synthetic polypeptides that, besides maintaining some properties of the native protein, can be tailored by fusing bioactive sequences to their C-terminal. Our laboratory synthesized several Human Elastin-Like Pol...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 19, 2018 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: December 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 12Author(s): (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 19, 2018 Category: Molecular Biology Source Type: research

A novel function for the ER retention signals in the C-terminus of kainate receptor subunit, GluK5
We report that GluK5 has two ER retention signals in its cytoplasmic C-terminus: an arginine-based signal and a di-leucine motif previously thought to be an endocytic motif. GluK5 assembles with GluK2, but surprisingly GluK2 association does little to block the ER retention signals. We find instead that the ER retention signals are blocked by two proteins involved in intracellular trafficking, SAP97 and CASK. We show that SAP97, in the presence of CASK and the receptor complex, assumes an extended conformation. In the extended conformation, SAP97 makes its SH3 and GuK domains available to bind and sterically mask the ER re...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 17, 2018 Category: Molecular Biology Source Type: research

(−)‑Oleocanthal inhibits proliferation and migration by modulating Ca2+ entry through TRPC6 in breast cancer cells
Publication date: Available online 12 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): R. Diez-Bello, I. Jardin, J.J. Lopez, M. El Haouari, J. Ortega-Vidal, J. Altarejos, G.M. Salido, S. Salido, J.A. RosadoAbstractTriple negative breast cancer is an aggressive type of cancer that does not respond to hormonal therapy and current therapeutic strategies are accompanied by side effects due to cytotoxic actions on normal tissues. Therefore, there is a need for the identification of anti-cancer compounds with negligible effects on non-tumoral cells. Here we show that (−)‑oleocan...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 13, 2018 Category: Molecular Biology Source Type: research

Protease-activated receptor 2 induces migration and promotes Slug-mediated epithelial-mesenchymal transition in lung adenocarcinoma cells
Publication date: Available online 13 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Chung-Che Tsai, Yu-Ting Chou, Hua-Wen FuAbstractProtease-activated receptor 2 (PAR2), a G protein-coupled receptor for trypsin, contributes to growth, anti-apoptosis, and migration in lung cancer. Given that PAR2 activation in airway epithelial cells compromises the airway epithelium barrier by disruption of E-cadherin adhesion, PAR2 may be involved in epithelial-mesenchymal transition (EMT) in lung adenocarcinoma cells. Although PAR2 is known to promote the migration of lung cancer cells, the d...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 13, 2018 Category: Molecular Biology Source Type: research

Identification of subcellular targeting sequences of Cten reveals its role in cell proliferation
Publication date: Available online 13 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Shiao-Ya Hong, Yi-Ping Shih, Abigail Lo, Su Hao LoAbstractSpatial and temporal subcellular localization plays critical roles in regulating protein function. Cten (C-terminal tensin like) is a member of the tensin family. Cten recruits signaling molecules, such as DLC1, to focal adhesions, modulates homeostasis of receptor tyrosine kinases, including EGFR and c-Met, and promotes cell migration. These functions are likely controlled by Cten localization at focal adhesions and/or in the cytoplasm. ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 13, 2018 Category: Molecular Biology Source Type: research

Cell size homeostasis: Metabolic control of growth and cell division
Publication date: Available online 11 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Mikael BjörklundAbstractJoint regulation of growth rate and cell division rate determines cell size. Here we discuss how animal cells achieve cell size homeostasis potentially involving multiple signaling pathways converging at metabolic regulation of growth rate and cell cycle progression. While several models have been developed to explain cell size control, comparison of the two predominant models shows that size homeostasis is dependent on the ability to adjust cellular growth rate base...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 11, 2018 Category: Molecular Biology Source Type: research

The cytosolic peroxisome-targeting signal (PTS)-receptors, Pex7p and Pex5pL, are sufficient to transport PTS2 proteins to peroxisomes
Publication date: Available online 5 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Satoru Mukai, Takashi Matsuzaki, Yukio FujikiAbstractProteins harboring peroxisome-targeting signal type-2 (PTS2) are recognized in the cytosol by mobile PTS2 receptor Pex7p and associate with a longer isoform Pex5pL of the PTS1 receptor. Trimeric PTS2 protein-Pex7p-Pex5pL complexes are translocated to peroxisomes in mammalian cells. However, it remains unclear whether Pex5pL and Pex7p are sufficient cytosolic components in transporting of PTS2 proteins to peroxisomes. Here, we construct a semi-i...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 6, 2018 Category: Molecular Biology Source Type: research

Cofilin-1 signaling mediates epithelial-mesenchymal transition by promoting actin cytoskeleton reorganization and cell-cell adhesion regulation in colorectal cancer cells
Publication date: Available online 5 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Annie Cristhine Moraes Sousa-Squiavinato, Murilo Ramos Rocha, Pedro Barcellos-de-Souza, Waldemir Fernandes Souza, Jose Andres Morgado-DiazAbstractColorectal cancer (CRC) is frequently a lethal disease because of metastasis. Actin cytoskeletal rearrangement is an essential step in cell migration during activation of the epithelial-mesenchymal transition (EMT) program, which is associated with metastatic properties of cancer cells. Cofilin-1 protein modulates actin dynamics by promoting actin tread...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 6, 2018 Category: Molecular Biology Source Type: research

Mutant p53 prevents GAPDH nuclear translocation in pancreatic cancer cells favoring glycolysis and 2-deoxyglucose sensitivity
Publication date: Available online 5 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Giovanna Butera, Raffaella Pacchiana, Nidula Mullappilly, Marilena Margiotta, Stefano Bruno, Paola Conti, Chiara Riganti, Massimo DonadelliAbstractPancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and devastating human malignancies. In about 70% of PDACs the tumor suppressor gene TP53 is mutated generally resulting in conformational changes of mutant p53 (mutp53) proteins, which acquire oncogenic functions triggering aggressiveness of cancers and alteration of energetic metabo...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 6, 2018 Category: Molecular Biology Source Type: research

Clostridium difficile toxin B induces senescence in enteric glial cells: A potential new mechanism of Clostridium difficile pathogenesis
In conclusion, TcdB induces senescence in EGCs. The extrapolation of these results to CDI leads to hypothesize that EGC that survived TcdB, once they have acquired a senescence state, could cause IBS and IBD due to persistent inflammation, transfer of senescence status and stimulation of pre-neoplastic cells.Graphical abstractToxin B f C. difficile (TcdB) induces in enteric glial cells (EGC) a senescence phenotype, which is dependent on p27 overexpression and activation of AKT and JNK, but independent on the p16/pRb and p53/p21 pathways as well as ROS. In callout are reported the main events induced by TcdB. (Source: Bioch...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 6, 2018 Category: Molecular Biology Source Type: research

NF-YA enters cells through cell penetrating peptides
In conclusion, NF-YA contains CPPs, hinting at novel -and unexpected- properties of this subunit. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 6, 2018 Category: Molecular Biology Source Type: research

From APOBEC to ZAP: Diverse mechanisms used by cellular restriction factors to inhibit virus infections
Publication date: Available online 2 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Mahesh Chemudupati, Adam D. Kenney, Serena Bonifati, Ashley Zani, Temet M. McMichael, Li Wu, Jacob S. YountAbstractAntiviral restriction factors are cellular proteins that inhibit the entry, replication, or spread of viruses. These proteins are critical components of the innate immune system and function to limit the severity and host range of virus infections. Here we review the current knowledge on the mechanisms of action of several restriction factors that affect multiple viruses at distinct ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 5, 2018 Category: Molecular Biology Source Type: research

Who guards the guardian? Mechanisms that restrain APC/C during the cell cycle
Publication date: Available online 2 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Jennifer Kernan, Thomas Bonacci, Michael J. EmanueleAbstractThe cell cycle is principally controlled by Cyclin Dependent Kinases (CDKs), whose oscillating activities are determined by binding to Cyclin coactivators. Cyclins exhibit dynamic changes in abundance as cells pass through the cell cycle. The sequential, timed accumulation and degradation of Cyclins, as well as many other proteins, imposes order on the cell cycle and contributes to genome maintenance. The destruction of many cell cycle r...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 5, 2018 Category: Molecular Biology Source Type: research