Metabolomics reveals impact of seven functional foods on metabolic pathways in a gut microbiota model.
Abstract Functional food defined as dietary supplements that in addition to their nutritional values, can beneficially modulate body functions becomes more and more popular but the reaction of the intestinal microbiota to it is largely unknown. In order to analyse the impact of functional food on the microbiota itself it is necessary to focus on the physiology of the microbiota, which can be assessed in a whole by untargeted metabolomics. Obtaining a detailed description of the gut microbiota reaction to food ingredients can be a key to understand how these organisms regulate and bioprocess many of these f...
Source: Adv Data - February 20, 2020 Category: Epidemiology Authors: Farag MA, Abdelwareth A, Sallam IE, El Shorbagi M, Jehmlich N, Fritz-Wallace K, Serena Schäpe S, Rolle-Kampczyk U, Ehrlich A, Wessjohann LA, von Bergen M Tags: J Adv Res Source Type: research

Effects of prickly pear (Opuntia ficus-indica L.) peel flour as an innovative ingredient in biscuits formulation
This study was aimed at assessing the aptitude of prickly pear peel flour to be mixed (10 g, 20 g, and 30 g/100 g) with wheat flour for biscuits preparation through the analysis of the physical and chemical properties of doughs and biscuits and through sensory evaluation.The composition of prickly pear flour showed a significantly higher concentration of fibre (20.70 g/100 g d.w.), ash (14.57 g/100 g d.w.), and phenolic compounds (277.6 mg/100 g d.w.) compared to the control wheat flour, thus improving technological properties such as the aptitude to kneading, the flavour retention, and the antioxid...
Source: LWT Food Science and Technology - February 19, 2020 Category: Food Science Source Type: research

Molecules, Vol. 25, Pages 916: Opuntia Fruits as Food Enriching Ingredient, the First Step towards New Functional Food Products
t-Miotła Prickly pear (Opuntia ficus indica (L.) Mill.) is a rich source of vitamins C, B1, B2, A, and E, minerals such as calcium, potassium, magnesium, iron, and phosphorus, as well as bioactive substances, i.e., carotenoids, betalains, and phenolic compounds. Of these, the phenolic acids, betalains, and flavonoids are notable in that they are largely responsible for the health-promoting properties of this plant. The purpose of the presented research was to first determine the antioxidant properties and the content of polyphenolic compounds (including individual phenolic acids) in prickly pear fruit, then to produce...
Source: Molecules - February 17, 2020 Category: Chemistry Authors: Anna Oniszczuk Agnieszka W ójtowicz Tomasz Oniszczuk Arkadiusz Matwijczuk Ahlem Dib Ewa Markut-Miot ła Tags: Communication Source Type: research

Identification and genomic characterization of a novel tobamovirus from prickly pear cactus
AbstractIn this work, we describe the complete sequence and genome organization of a novel tobamovirus detected in a prickly pear plant (Opuntia sp.) by high-throughput sequencing, tentatively named “opuntia virus 2”. The full genome of opuntia virus 2 is 6,453 nucleotides in length and contains four open reading frames (ORFs) coding for the two subunits of the RNA polymerase, the movement protein, and the coat protein, respectively. Phylogenetic analysis using the complete nucleotide seque nce revealed that the virus belongs to the genusTobamovirus (familyVirgaviridae), showing the highest nucleotide sequence identity...
Source: Archives of Virology - January 23, 2020 Category: Virology Source Type: research

Investigation of Amounts of Vitamins, Lycopene, and Elements in the Fruits of Opuntia ficus-indica Subjected to Different Pretreatments.
In this study, Opuntia ficus-indica fruit was examined in fresh, frozen, sun-dried, and microwave-dried. Analysis of the vitamins in the samples was performed by high performance liquid chromatography (HPLC). The amount of selenium in the fruits was determined by fluorescence spectrophotometer, while copper, iron, manganese, and zinc were determined by atomic absorption spectrophotometer (AAS). Amount of vitamins in the fruit of Opuntia ficus-indica were decreased (p < 0.005) in sun- and microwave-dried samples in comparison with fresh sample. Decrease of vitamins in frozen fruits was statistically insignificant com...
Source: Biological Trace Element Research - January 20, 2020 Category: Biology Authors: Bakar B, Çakmak M, Ibrahim MS, Özer D, Saydam S, Karatas F Tags: Biol Trace Elem Res Source Type: research

Metabolomics reveals impact of seven functional foods on metabolic pathways in a gut microbiota model
Publication date: Available online 3 January 2020Source: Journal of Advanced ResearchAuthor(s): Mohamed A. Farag, Amr Abdelwareth, Ibrahim E. Sallam, Mohamed el Shorbagi, Nico Jehmlich, Katarina Fritz, Stephanie schaepe, Ulrike Rolle-Kampczyk, Anja Ehrlich, Ludger A. Wessjohann, Martin von BergenAbstractFunctional food defined as dietary supplements that in addition to their nutritional values, can beneficially modulate body functions becomes more and more popular but the reaction of the intestinal microbiota to it is largely unknown. In order to analyse the impact of functional food on the microbiota itself it is necessar...
Source: Journal of Advanced Research - January 4, 2020 Category: Research Source Type: research

Determination of Antioxidant and Antifungal Activities in Cookies Fortified with Solar Dried Prickly Pear Peels Powder.
CONCLUSION: Addition of 5% dried cactus peel had lower overall quality and color than the control. Adding 3% of PPPP to cookies (qaraqeesh) showed the highest sensory score. Dried cactus peels may improve quality, nutritional value and shelf life of cookies. PMID: 32363815 [PubMed - in process] (Source: Pakistan Journal of Biological Sciences: PJBS)
Source: Pakistan Journal of Biological Sciences: PJBS - December 31, 2019 Category: Biomedical Science Authors: M Hegazy E, Ibrahim NM, S M Saleh N Tags: Pak J Biol Sci Source Type: research

Structural, luminescence and geno/cytoxicity study of carbon dots derived from Opuntia ficus-indica (L.) Mill
New J. Chem., 2020, Advance Article DOI: 10.1039/C9NJ03771C, PaperEduardo Madrigal-Bujaidar, Genaro Ivan Cer ón-Montes, Joan Reyes-Miranda, Erasto Vergara-Hernández, Isela Álvarez-González, Ángel de Jesús Morales-Ramírez, Luis Enrique Francisco-Martínez, Aristeo Garrido-Hernández Carbon dots derived from nopal significantly increase the number of micronuclei in mouse erythrocytes and inhibit mouse bone marrow cell proliferation. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - New J. Chem. latest articles)
Source: RSC - New J. Chem. latest articles - December 20, 2019 Category: Chemistry Authors: Eduardo Madrigal-Bujaidar Source Type: research

Opuntin B, the antiviral protein isolated from prickly pear (Opuntia ficus-indica (L.) Miller) cladode exhibits ribonuclease activity
Publication date: Available online 14 December 2019Source: Microbial PathogenesisAuthor(s): Rasoul Rasoulpour, Keramat Izadpanah, Alireza AfsharifarAbstractAn antiviral protein, designated Opuntin B, was purified from Prickly Pear (Opuntia ficus-indica (L.) Miller) Cladode by heat treatment of the extract, protein precipitation by ammonium sulfate treatment followed by ion-exchange chromatography. Assessment of enzymatic activity of the purified protein showed that it degrades total plant genomic RNA, while causing electrophoretic mobility shifting of Cucumber mosaic virus (CMV) RNAs. However, heat-denatured viral RNA beca...
Source: Microbial Pathogenesis - December 15, 2019 Category: Infectious Diseases Source Type: research

Enzyme-assisted supercritical fluid extraction of antioxidant isorhamnetin conjugates from Opuntia ficus-indica (L.) Mill
Publication date: Available online 4 December 2019Source: The Journal of Supercritical FluidsAuthor(s): Marilena Antunes-Ricardo, José A. Mendiola, Tomás García-Cayuela, Elena Ibañez, Janet A. Gutiérrez-Uribe, M. Pilar Cano, Daniel Guajardo-FloresAbstractA one-pot enzymatic assisted supercritical carbon dioxide (SC-CO2) extraction was used to obtain isorhamnetin conjugates from Opuntia ficus-indica (L.) Mill (nopal). Box-Benhken design, discriminate analysis and principal component analysis were used to studt the influence of pressure, temperature, aqueous ethanol solution and time on the total extraction yield and th...
Source: The Journal of Supercritical Fluids - December 5, 2019 Category: Chemistry Source Type: research

Classical and fortuitous biological control of the prickly pear cochineal, Dactylopius opuntiae, in Israel
Publication date: Available online 28 November 2019Source: Biological ControlAuthor(s): Zvi Mendel, Alexei Protasov, Juan M. Vanegas-Rico, J. Refugio Lomeli-Flores, Pompeo Suma, Esteban Rodríguez-LeyvaAbstractThe cochineal, Dactylopius opuntiae (Hemiptera: Dactylopiidae) was first reported in Israel in 2013 in a restricted area in the upper Galilee from where, by 2018, it had spread throughout Galilee and the northern Coastal Plain. It infests and kills the Indian-fig prickly pear, Opuntia ficus-indica (Cactaceae), a prominent plant in the landscape of Israel and other Mediterranean countries. Inundative releases of indus...
Source: Biological Control - November 29, 2019 Category: Biology Source Type: research

Structural, luminescence and Geno/cytoxicity study of carbon dots derived from Opuntia ficus-indica (L.) Mill.
New J. Chem., 2019, Accepted Manuscript DOI: 10.1039/C9NJ03771C, PaperEduardo Madrigal Bujaidar, Genaro Ivan Cer ón-Montes, Joan Reyes-Miranda, Erasto Vergara-Hernández, Isela Alvarez-Gonzalez, Angel de Jesus Morales-Ramírez, Luis Enrique Francisco-Martínez, Aristeo Garrido Hernández Photoluminescent carbon dots (CDs) were synthesized by the hydrothermal method. In the synthesis, different plants as carbon sources were used. Photoluminescence results demonstrated the highest emission for CDs coming from... The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - New J. Chem. latest articles)
Source: RSC - New J. Chem. latest articles - November 27, 2019 Category: Chemistry Authors: Eduardo Madrigal Bujaidar Source Type: research

Cholesterol transporter proteins in HepG2 cells can be modulated by phenolic compounds present in Opuntia ficus-indica aqueous solutions
This study tested the hypothesis that phenolic compounds can modulate the level of cholesterol transporters including Niemann-Pick C1-like 1 (NPC1L1) and ATP-binding cassette transporters in liver cells. HepG2 cells, used as a model of hepatocytes, showed a decrease in the abundance of cholesterol transporters comparatively to the control when treated with the Opuntia ficus-indica’s cladodes decoction. The decrease was between 13–70%, 25–60%, 9–60% and 23–60% for NPC1L1, ABCA1, ABCG5 and ABCG8 transporters, respectively, when using between 0.15 and 0.35 mg/mL of decoction in the culture medium. FTIR analysis sh...
Source: Journal of Functional Foods - November 16, 2019 Category: Nutrition Source Type: research

Isolation and characterization of opuntiol from Opuntia Ficus indica (L. Mill) and its antiproliferative effect in KB oral carcinoma cells.
In this study, we isolated and characterized a novel bioactive flavonol from the cactus pad of Opuntia Ficus indica Indica (L. Mill) (OFI) by chromatography techniques. The isolated compound was characterized by FT-IR, 1H and 13C NMR spectroscopy. Single-crystal XRD results illustrate that the obtained flavonol was opuntiol (6-hydroxymethyl-4-methoxy-2H-pyran-2-one) and it was found to be near planar except for the H atoms of the methylene and methyl groups. The crystal packing was stabilized by C-H….O and O-H….O intermolecular hydrogen bonds. The isolated opuntiol significantly inhibited KB cells proliferation and its...
Source: Natural Product Research - November 11, 2019 Category: Biochemistry Authors: Veeramani Kandan P, Dhineshkumar E, Karthikeyan R, Anbuselvan C, Maqbool I, Kanimozhi G, Arul Prakasam B, Rajendra Prasad N Tags: Nat Prod Res Source Type: research

Enzymatic hydrolysis of Opuntia ficus-indica cladode by Acinetobacter pittii and alcohol fermentation by Kluyveromyces marxianus: pH, temperature and microorganism effect
Publication date: Available online 25 October 2019Source: Biotechnology ReportsAuthor(s): C.M. López-Domínguez, M.O. Ramírez-Sucre, I.M. Rodríguez-BuenfilAbstractIn the present work the effect of three factors: pH, temperature and type of microorganism using a factorial design 32x2 was evaluated on: growth, total cellulases hydrolytic activity (FPases), endoglucanases hydrolytic activity (CMCases), free reducing sugars (FRS), glucose, sucrose, and alcohol production using a culture medium based on mineral salts added with cladodes flour of Opuntia ficus-indica at 20% as the unique carbon source. Two wild microorganisms...
Source: Biotechnology Reports - October 26, 2019 Category: Biotechnology Source Type: research