Biophysical Characterization of Biopharmaceuticals, Including Antibody Drugs.
Abstract   Biopharmaceuticals, including antibody drugs, are now popular because of their high specificity with low adverse effects, especially in the treatment of cancer and autoimmune diseases. However, because the active pharmaceutical ingredients of biopharmaceuticals are proteins, biophysical characterization of these therapeutic proteins should be required. In this manuscript, methods of chemical and physical characterization of therapeutic proteins are described. In terms of chemical characterization, analysis of chemical modifications of the constituent amino acids is explained. Physical characte...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - March 6, 2016 Category: Drugs & Pharmacology Authors: Uchiyama S Tags: Yakugaku Zasshi Source Type: research

Structural Study in the Platform for Drug Discovery, Informatics, and Structural Life Science.
Abstract   The Platform for Drug Discovery, Informatics, and Structural Life Science (PDIS), which has been launched since FY2012, is a national project in the field of structural biology. The PDIS consists of three cores - structural analysis, control, and informatics - and aims to support life science researchers who are not familiar with structural biology. The PDIS project is able to provide full-scale support for structural biology research. The support provided by the PDIS project includes protein purification with various expression systems, large scale protein crystallization, crystal structure d...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - March 6, 2016 Category: Drugs & Pharmacology Authors: Senda T Tags: Yakugaku Zasshi Source Type: research

Novel Therapeutic Targets for Diseases of Endocrine or Immune System Developed through the Analysis of Cross-talk between Ca(2+) and Disease-related Cell Signaling.
PMID: 26935086 [PubMed - in process] (Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan)
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - March 6, 2016 Category: Drugs & Pharmacology Authors: Ishikawa T, Ohya S Tags: Yakugaku Zasshi Source Type: research

Regulation of Lipid Metabolism by Diacylglycerol Kinases in Pancreatic β-cells.
Regulation of Lipid Metabolism by Diacylglycerol Kinases in Pancreatic β-cells. Yakugaku Zasshi. 2016;136(3):461-5 Authors: Kaneko YK, Ishikawa T Abstract   The appropriate secretion of insulin from pancreatic β-cells is essential for regulating blood glucose levels. Glucose-stimulated insulin secretion (GSIS) involves the following steps: Glucose uptake by pancreatic β-cells is metabolized to produce ATP. Increased ATP levels result in the closure of ATP-sensitive K(+) (KATP) channels, resulting in membrane depolarization that activates voltage-dependent Ca(2+) channels to su...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - March 6, 2016 Category: Drugs & Pharmacology Authors: Kaneko YK, Ishikawa T Tags: Yakugaku Zasshi Source Type: research

Theoretical Investigations into the Quantitative Mechanisms Underlying the Regulation of [cAMP]i, Membrane Excitability and [Ca(2+)]i during GLP-1 Stimulation in Pancreatic β Cells.
Theoretical Investigations into the Quantitative Mechanisms Underlying the Regulation of [cAMP]i, Membrane Excitability and [Ca(2+)]i during GLP-1 Stimulation in Pancreatic β Cells. Yakugaku Zasshi. 2016;136(3):467-71 Authors: Takeda Y Abstract   Upon elevation of plasma glucose concentration, pancreatic β-cells generate bursts of action potentials to induce cyclic changes in [Ca(2+)]i regulating insulin release. Glucose-dependent insulin secretion is synergistically enhanced by glucagon-like peptide-1 (GLP-1), which increases [cAMP]i and activates protein kinase A (PKA) and exchan...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - March 6, 2016 Category: Drugs & Pharmacology Authors: Takeda Y Tags: Yakugaku Zasshi Source Type: research

Store-operated Calcium Entry into B Cells Regulates Autoimmune Inflammation.
Abstract   Alterations in the cytosolic concentration of calcium ions (Ca(2+)) are important signals for various physiological events. The engagement of B cell receptors (BCR) results in the transient release of Ca(2+) into cytosol from endoplasmic reticulum (ER) stores. In turn, this decrease in ER luminal Ca(2+) concentration triggers the opening of Ca(2+) channels in the plasma membrane, inducing a sustained influx of extracellular Ca(2+) into cells. These processes are referred to as store-operated Ca(2+) entry (SOCE), which is an essential pathway for continuous Ca(2+) signaling. While the ER calciu...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - March 6, 2016 Category: Drugs & Pharmacology Authors: Baba Y Tags: Yakugaku Zasshi Source Type: research

Physiological Role of K(+) Channels in the Regulation of T Cell Function.
Abstract   Potassium ion (K(+)) channels play an important role in the modulation of calcium ion (Ca(2+)) signaling via control of the membrane potential. In T-lymphocytes, the voltage-gated K(+) channel, KV1.3, and the intermediate-conductance Ca(2+)-activated K(+) channel, KCa3.1, predominantly contribute to K(+) conductance, and are responsible for cell proliferation, differentiation, apoptosis and infiltration. Inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease, afflicts more than 0.1% of the population worldwide. In the chemically-induced IBD model mouse, an increase ...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - March 6, 2016 Category: Drugs & Pharmacology Authors: Ohya S Tags: Yakugaku Zasshi Source Type: research

Functional Expression of a Ca(2+)-activated Cl(-) Channel Modulator Involved in Ion Transport and Epithelial Cell Differentiation.
Abstract   Cl(-)-permeable channels and transporters expressed on the cell membranes of various mammalian cell types play pivotal roles in the transport of electrolytes and water, pH regulation, cell volume and membrane excitability, and are therefore expected to be useful molecular targets for drug discovery. Both TMEM16A (a possible candidate for Ca(2+)-regulated Cl(-) channels recently identified) and cystic fibrosis transmembrane conductance regulator (CFTR) (or cAMP-regulated Cl(-) channels) have been known to be involved in Cl(-) secretion and reabsorption in the rat salivary gland. Crosstalk betwe...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - March 6, 2016 Category: Drugs & Pharmacology Authors: Yamazaki J Tags: Yakugaku Zasshi Source Type: research

Development of Molecular Probes for Spatio-temporal Analysis of in Vivo Tumor with Photoacoustic Imaging.
Abstract   Photoacoustic imaging (PA imaging or PAI) has been focused on as a new technique to provide images of high spatial resolution, at depths of up to 5 cm, and the development of novel PAI probes for tumor imaging is of marked interest. Although nanomaterials such as gold nanorods have been reported as PAI probes, dyes are required to aid their ease of preparation, cost-effectiveness, and safety. However, because PAI has relatively low intrinsic sensitivity compared to optical imaging, and requires high-energy laser pulse exposure, an appropriate probe design, high tumor accumulation, and photosta...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - March 6, 2016 Category: Drugs & Pharmacology Authors: Onoe S Tags: Yakugaku Zasshi Source Type: research

Methodology for Estimating the Risk of Adverse Drug Reactions in Pregnant Women: Analysis of the Japanese Adverse Drug Event Report Database.
Abstract   Safety information regarding drug use during pregnancy is insufficient. The present study aimed to establish an optimal signal detection method to identify adverse drug reactions in pregnant women and to evaluate information in the Japanese Adverse Drug Event Report (JADER) database between April 2004 and November 2014. We identified reports on pregnant women using the Standardised MedDRA Queries. We calculated the proportional reporting ratio (PRR) and reporting odds ratio (ROR) of the risk factors for the two known risks of antithyroid drugs and methimazole (MMI) embryopathy, and ritodrine a...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - March 6, 2016 Category: Drugs & Pharmacology Authors: Sakai T, Ohtsu F, Sekiya Y, Mori C, Sakata H, Goto N Tags: Yakugaku Zasshi Source Type: research

Evaluation of the Association of Hand-Foot Syndrome with Anticancer Drugs Using the US Food and Drug Administration Adverse Event Reporting System (FAERS) and Japanese Adverse Drug Event Report (JADER) Databases.
Abstract   The Japanese Ministry of Health, Labor, and Welfare lists hand-foot syndrome as a serious adverse drug event. Therefore, we evaluated its association with anticancer drug therapy using case reports in the Japanese Adverse Drug Event Report (JADER) and the US Food and Drug Administration (FDA) Adverse Event Reporting System (FAERS). In addition, we calculated the reporting odds ratio (ROR) of anticancer drugs potentially associated with hand-foot syndrome, and applied the Weibull shape parameter to time-to-event data from JADER. We found that JADER contained 338224 reports from April 2004 to No...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - March 6, 2016 Category: Drugs & Pharmacology Authors: Sasaoka S, Matsui T, Abe J, Umetsu R, Kato Y, Ueda N, Hane Y, Motooka Y, Hatahira H, Kinosada Y, Nakamura M Tags: Yakugaku Zasshi Source Type: research

Stabilization of the Serum Lithium Concentration by Regulation of Sodium Chloride Intake: Case Report.
Abstract   To avoid fluctuation of the serum lithium concentration (CLi), sodium chloride (NaCl) intake was regulated in oral alimentation. A 62-year-old woman was hospitalized and orally administered 400 mg of lithium carbonate a day to treat her mania. Her CLi was found to be 0.75-0.81 mEq/L. Vomiting made it difficult for the patient to ingest meals orally, and therefore parenteral nutrition with additional oral intake of protein-fortified food was initiated. On day 22, parenteral nutrition was switched to oral alimentation to enable oral intake of food. The total NaCl equivalent amount was decreased ...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - March 6, 2016 Category: Drugs & Pharmacology Authors: Tomita T, Goto H, Sumiya K, Yoshida T, Tanaka K, Kohda Y Tags: Yakugaku Zasshi Source Type: research

Promotion of Drug Discovery Research by Utilizing Omics Technology.
PMID: 26831783 [PubMed - in process] (Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan)
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Nagano K, Higashisaka K Tags: Yakugaku Zasshi Source Type: research

Challenge to the Development of Molecular Targeted Therapy against a Novel Target Candidate Identified by Antibody Proteomics Technology.
Abstract   Disease proteomics that systemically analyzes and identifies differentially expressed proteins between healthy and diseased samples is a potentially useful approach for obtaining target proteins for drug development. To date, however, very few target proteins have been identified from this field. A key issue that remains to be resolved is how to correctly identify target proteins from a number of potential candidates. To circumvent this problem, we have developed "antibody proteomics technology" in which a single chain Fv phage antibody library is utilized for proteome analysis. Here...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Nagano K Tags: Yakugaku Zasshi Source Type: research

Functional Analysis of GATA4 Complex, a Cardiac Hypertrophy-response Transcriptional Factor, Using a Proteomics Approach.
Abstract   Various stresses on the heart, such as myocardial infarction and hemodynamic overload, activate the sympathetic nervous system and the renin-angiotensin system, ultimately reach the nuclei of cardiomyocytes, and change the pattern of gene expression associated with cardiac hypertrophy. Although present pharmacological therapy for heart failure targets such extracellular molecules, mortality due to heart failure is still high. A zinc finger protein, GATA4, is one of the hypertrophy-responsive transcription factors, forms a functional protein complex with an intrinsic histone acetyltransferase, ...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Sunagawa Y, Katanasaka Y, Wada H, Hasegawa K, Morimoto T Tags: Yakugaku Zasshi Source Type: research

Screening for Potential Drug Targets by Comprehensive Identification of Disease-specific Antigens Incorporated into Immune Complexes in Patients with Immunological Diseases.
Abstract   Our immune system resembles an intelligent security system, which continually monitors for foreign invaders (infectious diseases); however, in some cases, this system recognizes healthy parts as something harmful or foreign and then attacks them (autoimmune diseases). The defining characteristics of an autoimmune disease are the existence of T- and B-cell autoreactivity against self proteins (autoantigens). In addition to autoimmune diseases, aberrant host proteins that occur during a certain state of diseases (e.g., cancer) can be recognized as an autoantigen. Immune complexes (ICs) are produ...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Ohyama K Tags: Yakugaku Zasshi Source Type: research

Application of Imaging Mass Spectrometry for Drug Discovery.
Abstract   Imaging mass spectrometry (IMS) can reveal the distribution of biomolecules on tissue sections. In this process, the biomolecules are directly ionized within tissue sections using matrix-assisted laser desorption/ionization, and then their distribution is visualized by pseudo-color based on the relative signal intensity. The biomolecules, such as fatty acids, phospholipids, glycolipids, peptides, proteins, and neurotransmitters, have been analyzed at a spatial resolution of 5 μm. A special instrument for IMS analysis was developed by Shimadzu. The IMS analysis does not require the labeling ...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Hayasaka T Tags: Yakugaku Zasshi Source Type: research

Structural Pharmacology for Receptors and Transporters.
PMID: 26831788 [PubMed - in process] (Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan)
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Shimizu T, Kato H Tags: Yakugaku Zasshi Source Type: research

Structural Analyses of Toll-like Receptor Sensing Single-stranded Nucleic Acids and Its Application.
We describe the crystal structure of a newly developed agonist, C2-butyl furo[2,3-c]quinolone. PMID: 26831789 [PubMed - in process] (Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan)
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Shimizu T Tags: Yakugaku Zasshi Source Type: research

Structural Life Science towards the Regulation of Selective GPCR Signaling.
Abstract   G protein-coupled receptors (GPCRs) are the largest family of receptors in the human genome. They are involved in many diseases and also the target of approximately 30% of all modern medicinal drugs. GPCRs respond to a broad spectrum of chemical entities, ranging from photons, protons, and calcium ions to small organic molecules (including odorants and neurotransmitters), peptides, and glycoproteins. Many GPCRs are members of closely related subfamilies that respond to the same hormone or neurotransmitter. However, they have different physiologic functions based on the cells in which they are ...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Kobayashi T Tags: Yakugaku Zasshi Source Type: research

Structural and Functional Studies on Photoactive Retinal Proteins: Light Becomes Drugs with Proteins.
Abstract   Retinal proteins possess vitamin A aldehyde (retinal) as a chromophore within seven transmembrane α-helices. Visible light absorption of them triggers trans-cis photoisomerization of the retinal chromophore and induces structural changes in the protein moiety, resulting in a variety of biological functions such as vision, ion transportation, and photosensing. Environmental genomics revealed that retinal proteins are widely distributed through all three biological kingdoms, eukarya, bacteria, and archaea, indicating the biological significance of their light energy conversion. In addition...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Sudo Y Tags: Yakugaku Zasshi Source Type: research

In Vitro Selected Macrocyclic Peptides: Tools for Regulating the Conformational Freedom of Transmembrane Proteins.
Abstract   Membrane proteins allow a cell to communicate with its environment by relaying a signal or transporting a molecule through the cell membrane. Elucidation of the three-dimensional structure of a membrane protein provides a greater understanding of its function and mechanisms. Ultimately, this knowledge will enlighten researchers on how these proteins can be regulated to elicit a desired cellular response, which could lead to novel therapeutic medicine. Unfortunately, the determination of the high-resolution crystal structures of transmembrane proteins remains a challenge due to their poor solub...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Hipolito CJ, Nishio K, Suga H Tags: Yakugaku Zasshi Source Type: research

Structural and Pharmacological Studies of an ABC Multidrug Transporter.
Abstract   A drug's effectiveness against a disease depends not only on its interaction with receptors but also its pharmacokinetics (absorption, distribution, metabolism, and extrusion; ADME). ATP binding cassette (ABC) multidrug transporters are important proteins that influence the ADME properties of a drug, especially the ABC transporter subfamily B member 1 (ABCB1). Elucidation of the molecular mechanisms of ABCB1 will contribute to our understanding of the molecular basis of ADME. Human ABCB1 is expressed in many organelles, and exports various substrates from cells using energy generated by its AT...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Yamaguchi T Tags: Yakugaku Zasshi Source Type: research

The Challenge of Developing New Treatments for NTDs Originating from Japan.
PMID: 26831794 [PubMed - in process] (Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan)
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Kita K, Yamada H Tags: Yakugaku Zasshi Source Type: research

Current Trend of Drug Development for Neglected Tropical Diseases (NTDs).
Abstract   EBOLA hemorrhagic fever, a typical emerging infectious disease, began in December 2013 in the southern part of Guinea, and killed more than 11000 people by the end of June, 2015. In addition to emerging/re-emerging diseases and the 3 major infectious diseases i.e. HIV/AIDS, tuberculosis and malaria, neglected tropical diseases (NTDs) have recently become important tropical diseases of the poor. It is remarkable that Japan succeeded in the eradication of malaria and other tropical diseases, which include lymphatic filariasis and schistosomiasis. However, despite these achievements, it is import...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Kita K Tags: Yakugaku Zasshi Source Type: research

International Partnership for Therapeutic Drug Development of NTDs by DNDi.
r C Abstract   The Drugs for Neglected Diseases initiative (DNDi), with headquarters in Geneva, is a non-profit drug research and development (R&D) organization and Product Development Partnership (PDP) which was established in 2003 by 7 founding organizations such as Médecins Sans Frontières (MSF), the Pasteur Institute, The Specific Programme for Research and Training in Tropical Diseases (WHO-TDR), etc. DNDi has worked mainly on the development of new treatments for neglected tropical diseases (NTDs), which is difficult to achieve under market economy conditions. DNDi has promoted ov...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Yamada H, Hirabayashi F, Brünger C Tags: Yakugaku Zasshi Source Type: research

Development and Distribution of Drugs for NTDs: Efforts of One Pharmaceutical Company.
Abstract   The Pharmaceutical Industry is expected to play a proactive global role in combatting neglected tropical diseases (NTDs) and other tropical diseases affecting low-income countries. Such a role would include novel medicine R&D, manufacturing and distribution. In order to succeed in this role, several challenges need to be overcome: a) the economic challenge or cost benefit balance for the development of these medicines, and b) sparse in-house experience with these diseases within the Industry. During the last decade, the Product Development Partnership (PDP) model has become an effective st...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Asada M Tags: Yakugaku Zasshi Source Type: research

Activity of NTDs Drug-discovery Research Consortium.
Abstract   Neglected tropical diseases (NTDs) are an extremely important issue facing global health care. To improve "access to health" where people are unable to access adequate medical care due to poverty and weak healthcare systems, we have established two consortiums: the NTD drug discovery research consortium, and the pediatric praziquantel consortium. The NTD drug discovery research consortium, which involves six institutions from industry, government, and academia, as well as an international non-profit organization, is committed to developing anti-protozoan active compounds for three NT...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Namatame I Tags: Yakugaku Zasshi Source Type: research

Industry, Academia and Government Partnership through the Global Health Innovative Technology Fund (GHIT).
Abstract   In developing countries, many people are unable to access basic healthcare services, resulting in many avoidable deaths and/or disabilities. The United Nations adopted the Millennium Development Goals in order to resolve this problem, and Japan has been contributing greatly to the achievement of these goals. In this context, in 2013 the Government of Japan proposed its Strategy on Global Health Diplomacy, and since then has been promoting Universal Health Coverage. Since the beginning of the 21st century, the particular importance of addressing neglected tropical diseases (NTDs) has been stres...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Hinoshita E Tags: Yakugaku Zasshi Source Type: research

Establishment for the Supporting of Pharmacies as the Healthcare Information Hub in the Community.
PMID: 26831800 [PubMed - in process] (Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan)
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Saito M, Yamamoto M Tags: Yakugaku Zasshi Source Type: research

Current Status of Community Pharmacies: Expectations as a Health Information Hub, the Enforcement of Revised Pharmaceutical and Medical Device Act, and a New Role as Stakeholders.
Abstract   According to the "Japan Revitalization Strategy" established in June 2013, "the government will promote better contributions of local pharmacies and pharmacists in encouraging self-medication of citizens by making pharmacies the community-based hub for providing information, giving advice on the proper use of non-prescription drugs, etc. and offering consultation and information service concerning health". In addition, the "Demanded Function and Ideal Form of Pharmacy," published in January 2014, requested a change, from pharmacies that specialized in dispensing m...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Saito M Tags: Yakugaku Zasshi Source Type: research

Japanese Model of Asheville Project. Its Overview and Initiative.
Abstract   The Asheville Project(®) began in 1996 in Asheville, North Carolina, where community pharmacists, in collaboration with physicians, provide health coaching to patients with lifestyle diseases to accomplish their improved self-management of the disease. The project has now widely expanded across the United States. With periodical coaching by pharmacists, according to the reports of these programs, patients have been reported to show improvements in self-management and laboratory data, including the number of doctor visits, medication adherence and the number of foot examinations. Economical...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Furukawa A, Asada Y, Mori T, Inoue G, Atsuda K Tags: Yakugaku Zasshi Source Type: research

Pilot Study of "Japanese Model of Asheville Project"~How Did It Work?
This study evaluated the two primary measurements, which are A) the actual execution rate versus planned programs, and B) the patient satisfaction rate. In addition, the achievement rate of behavioral objectives, satisfaction rate of diabetes treatment, degree of concerns (Diabetes Treatment Satisfaction Questionnaire; DTSQ, Problem Areas in Diabetes; PAID) and other physiological indicators have been evaluated. With the approval of the IRB at Kitasato University, sixteen out of 18 patients have continued to participate in the support program, and the study has shown high patient satisfaction with pharmacist coaching suppo...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Fujii H, Mori T, Furukawa A, Inoue G, Atsuda K Tags: Yakugaku Zasshi Source Type: research

Estimation of the Health Status of People in the Vicinity of Pharmacies Using Pharmacy Big Data.
Abstract   The purpose of this study was to propose a method for visualizing the patterns of the geographical propagation of influenza infection, and to elaborate parameters for the characterization of these patterns. First, a motion picture was prepared for the quotidian propagation of influenza infection in the Greater Tokyo Metropolitan area, which is considered a typical epidemic area for the 2012/2013 flu season. Second, hebdomadal recordings of patients with influenza infection in the 47 prefectures of Japan were grouped into 3 categories (1-peak, 2-peak, or multi-peak). The prefectures were arrang...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Hayashi Y, Saito M, Yajima T Tags: Yakugaku Zasshi Source Type: research

Development of an Informative System for Consumers and Pharmacists Aimed at the Proper Use of Kampo OTCs.
Abstract   Kampo medicines must be used according to an individual's physical characteristics and symptoms to avoid lack of efficacy, adverse reactions or interactions with other drugs. As category-2 over-the-counter drugs (OTCs), Kampo OTCs are not targets of active explanations by pharmacists, and consumers usually decide to use OTCs by themselves on the basis of drug label information. However, information on the label is occasionally brief. To promote the proper use of Kampo OTCs, we herein propose an informative tool based on the Australian Shelf-Talker (information on the proper use of OTCs with a ...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Takano-Ohmuro H, Nakajima Y, Saito M, Yuda Y Tags: Yakugaku Zasshi Source Type: research

For the Establishment of an Informative Support Framework in Pharmacies: Informative Support System for Diabetes.
Abstract   According to the Japanese revitalization strategy endorsed by the government in June, 2013, pharmacies are expected to play an active role as the hub of health information. But this is not sufficiently organized: an infrastructure for providing neutral information which becomes the basis of such health information is not yet established for healthcare professionals, patients and consumers. As for drug information available subsequent to the marketing of pharmaceutical products, information from the pharmaceutical companies including Package Inserts and Interview-forms are often found. However,...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Yamamoto M, Doi H, Watanabe K Tags: Yakugaku Zasshi Source Type: research

Role of Transient Receptor Potential Channels in Paclitaxel- and Oxaliplatin-induced Peripheral Neuropathy.
Abstract   Peripheral neuropathy is a common adverse effect of paclitaxel and oxaliplatin treatment. The major dose-limiting side effect of these drugs is peripheral sensory neuropathy. The symptoms of paclitaxel-induced neuropathy are mostly sensory and peripheral in nature, consisting of mechanical allodynia/hyperalgesia, tingling, and numbness. Oxaliplatin-induced neurotoxicity manifests as rapid-onset neuropathic symptoms that are exacerbated by cold exposure and as chronic neuropathy that develops after several treatment cycles. Although many basic and clinical researchers have studied anticancer dr...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Taguchi K Tags: Yakugaku Zasshi Source Type: research

Structure and Function of the Nuclear Receptor Constitutive Androstane Receptor.
Abstract   Animal defense mechanisms against both endogenous and exogenous toxic compounds function mainly through receptor-type transcription factors, including the constitutive androstane receptor (CAR). Following xenobiotic stimulation, CAR translocates into the nucleus and transactivates its target genes including oxygenic and conjugative enzymes and transporters in hepatocytes. We identified subcellular localization signals in the rat CAR: two nuclear localization signals (NLS1 and 2); two nuclear export signals (NES1 and 2); and a cytoplasmic retention region. The nuclear import of CAR is regulated...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Inouye Y Tags: Yakugaku Zasshi Source Type: research

Diversity of Plants Belonging to the Genus Ligularia (Asteraceae) Based on Terpenoids and Synthetic Studies on Some Terpenoids.
Abstract   The terpenoid constituents of Ligularia virgaurea (30 samples), Ligularia pleurocaulis (8 samples), Ligularia dictyoneura (8 samples), Ligularia brassicoides (5 samples), Ligularia lingiana (1 sample), and Ligularia liatroides (1 sample)(all belonging to section Senecillis of Ligularia, Asteraceae and collected in Yunnan, Sichuan, Qinghai, and Gansu provinces, China), from which 220 compounds were isolated, including 113 novel ones, are reviewed. Five chemotypes were identified in L. virgaurea based on their chemical constituents, while three clades were detected from the base sequences. Altho...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Tori M Tags: Yakugaku Zasshi Source Type: research

Study of Supplementary Analgesics Capable of Reducing the Dosage of Morphine.
This article reviews the potential mechanism of spinally mediated nociceptive behaviors evoked by i.t. M3G in mice. We discuss the possible presynaptic release of nociceptive neurotransmitters/neuromodulators such as substance P, glutamate, dynorphin, and Leu-enkephalin in the primary afferent fibers following i.t. M3G administration. It is possible to speculate that i.t. M3G could indirectly activate NK1, NMDA, and δ2-opioid receptors that lead to the release of nitric oxide (NO) in the dorsal spinal cord. The major function of NO is the production of cGMP and the activation of protein kinase G (PKG). The NO-cGMP-PK...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Komatsu T Tags: Yakugaku Zasshi Source Type: research

Implementation and Evaluation of Genetic Testing Seminars on Lifestyle-related Disease Prevention for Pharmacy Students.
Abstract   The field of pharmacotherapy has advanced to use molecular targeted agents, and pharmacists are now encouraged to focus on pharmacogenomics. A seminar titled "Implementation and evaluation of genetic testing of lifestyle-related disease genes" was presented to students at the Faculty of Pharmaceutical Sciences, Hiroshima University, describing the pharmacogenomic role of single nucleotide polymorphisms in obesity-related genes and alcohol metabolism-related genes. The seminar topic was selected first because pharmaceutical students were already familiar with some of the content, and ...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Ikeda K, Hosoi T, Yoshii M, Sugiyama M, Ozawa K Tags: Yakugaku Zasshi Source Type: research

Pharmacists' Behavior in Clinical Practice: Results from a Questionnaire Survey of Pharmacy Students.
Abstract   A questionnaire survey was performed to obtain pharmacy students' impressions of pharmacists' behavior, to classify these based on professionalism, and to analyze the relationship between these experiences and students' satisfaction with their clinical practice in Japan. The questionnaire was answered by 327 5th-year pharmacy school students upon completing clinical practice at community pharmacies from 2011 to 2012. They rated their satisfaction with their clinical practice using a 6-point Likert scale, and provided descriptions of their experience such as, "This health provider is profe...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - February 3, 2016 Category: Drugs & Pharmacology Authors: Nakada A, Akagawa K, Yamamoto H, Kato Y, Yamamoto T Tags: Yakugaku Zasshi Source Type: research

Chamical Biology for Pharmaceutical Sciences—Novel Approaches for Exploring Cellular Functions with Non-drug Chemicals.
Chamical Biology for Pharmaceutical Sciences—Novel Approaches for Exploring Cellular Functions with Non-drug Chemicals. Yakugaku Zasshi. 2016;136(1):1 Authors: Ojida A, Nakagawa H PMID: 26725659 [PubMed - in process] (Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan)
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - January 13, 2016 Category: Drugs & Pharmacology Authors: Ojida A, Nakagawa H Tags: Yakugaku Zasshi Source Type: research

Development of Fluorescence Sensing Mechanism for Cell Functional Analysis.
Abstract   Fluorescence probes are now widely used as indispensable tools in many cell functional analyses. At present, the design of fluorescent probes largely depends on the limited numbers of established sensing mechanisms such as photo-induced electron transfer (PET), photo-induced charge transfer (PCT), and fluorescence resonance energy transfer (FRET). Although these mechanisms are versatile in metal ion sensing, introduction of a new sensing mechanism is highly desirable not only to design a more sophisticated probe with high selectivity and sensitivity but also to expand the diversity of ...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - January 13, 2016 Category: Drugs & Pharmacology Authors: Ojida A, Takashima I Tags: Yakugaku Zasshi Source Type: research

Small-molecule Ligands That Manipulate the Intracellular Location of Proteins.
Abstract   Synthetic small-molecule ligands that control the intracellular location of proteins would be powerful tools for regulating cellular systems. However, the creation of such molecules has long remained unexplored because of the lack of a design methodology. Here, we introduce a new type of synthetic ligands, self-localizing ligands (SLLs), which spontaneously localize to specific subcellular regions in mammalian cells. We show that SLLs bind their target (exogenously expressed and endogenous) proteins and relocate them rapidly from the cytoplasm to their targeting sites. SLL-induced prot...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - January 13, 2016 Category: Drugs & Pharmacology Authors: Tsukiji S Tags: Yakugaku Zasshi Source Type: research

The Development of Luminescent Nano-probes on Hard X-ray Irradiation.
Abstract   X-rays are widely used in imaging applications such as diffraction imaging of crystals and medical imaging. In particular, X-ray computed tomography (CT) is a critical tool for clinical and disease diagnostics. The principle of conventional CT is based on X-ray attenuation caused by photoelectric absorption and scattering. In addition to conventional CT, a number of novel methodologies are presently under development, including state-of-the-art instrument technologies and chemical probes to fulfill diagnosis criteria. Among these novel methodologies, we have utilized hard X-ray-excited...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - January 13, 2016 Category: Drugs & Pharmacology Authors: Osakada Y Tags: Yakugaku Zasshi Source Type: research

Visualization and Functional Regulation of Live Cell Proteins Based on Labeling Probe Design.
Abstract   There are several approaches to understanding the physiological roles of biomolecules: (1) by observing the localization or activities of biomolecules (based on microscopic imaging experiments with fluorescent proteins or fluorescent probes) and (2) by investigating the cellular response via activation or suppression of functions of the target molecule (by using inhibitors, antagonists, siRNAs, etc.). In this context, protein-labeling technology serves as a powerful tool that can be used in various experiments, such as for fluorescence imaging of target proteins. Recently, we developed...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - January 13, 2016 Category: Drugs & Pharmacology Authors: Mizukami S, Kikuchi K Tags: Yakugaku Zasshi Source Type: research

Caged Gaseous Mediators and Their Control of Cellular Functions.
Abstract   Nitric oxide (NO) is historically well known as a toxic gas but now recognized as a physiological cellular mediator acting at very low concentrations. It is biosynthesized within the body, and modulates many signal transduction processes. For investigation of the functions of this gaseous mediator, it is necessary to use chemical donors that release NO specifically, and it is highly advantageous if the release can be made with precise spatiotemporal control. For this purpose, we have developed caged NO (photocontrollable NO-releasing compounds) with unique releasing mechanisms. One emp...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - January 13, 2016 Category: Drugs & Pharmacology Authors: Nakagawa H Tags: Yakugaku Zasshi Source Type: research

Frontier Pharmaceutics to Be Explored by Young Scientists: DDS Research for Innovative Drug Development.
PMID: 26725665 [PubMed - in process] (Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan)
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - January 13, 2016 Category: Drugs & Pharmacology Authors: Asai T, Tachikawa M Tags: Yakugaku Zasshi Source Type: research

Human Serum Albumin as Carrier in Drug Delivery Systems.
Abstract   Recently, human serum albumin (HSA) has emerged as a versatile carrier for therapeutic agents against diabetes, cancer, and infectious diseases. Market-approved products include fatty acid derivatives of human insulin for diabetes and the paclitaxel-HSA nanoparticle for various cancers such as metastatic breast cancer and advanced pancreatic cancer. In this review, we focus on the next-generation approach including HSA-binding bioactive gas such as nitric oxide (NO) for treating ischemic/reperfusion injury, cancer, and bacterial infection. To date, pharmacologically active compounds th...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - January 13, 2016 Category: Drugs & Pharmacology Authors: Ishima Y, Maruyama T Tags: Yakugaku Zasshi Source Type: research