PharmaNews
Transfus Med Hemother 2021;48:69 –70 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - February 1, 2021 Category: Hematology Source Type: research

In the Mood for a Blood Donation? Pilot Study about Momentary Mood, Satisfaction, and Return Behavior in Deferred First-Time Donors
Conclusion: Our findings suggest that mood and satisfaction with the donation process are directly affected by deferral but not clearly responsible for low return rates. It seems promising to embed these variables in established health behavior models in further studies to increase the return rates of deferred FTD.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - January 26, 2021 Category: Hematology Source Type: research

Coagulation under Mild Hypothermia Assessed by Thromboelastometry
Conclusion: This demonstrates that even under mild hypothermia coagulation is significantly altered in vitro. Perioperative temperature monitoring and management are greatly important and can help to prevent mild hypothermia and its adverse effects. Further investigation and in vivo testing of coagulation under mild hypothermia is needed.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - January 14, 2021 Category: Hematology Source Type: research

Safety Aspects in Tissue Banking – An Update
Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - January 14, 2021 Category: Hematology Source Type: research

A Para-Bombay Blood Group Case Associated with a Novel < b > < i > FUT1 < /i > < /b > Mutation c.361G & #x3e;A
Conclusions: A novelFUT1 allele (FUT1*c.361G#x3e;A) was identi fied in a Chinese individual with para-Bombay B phenotype. TheFUT1c.361G#x3e;A mutation may significantly downregulate the expression of H antigens on RBCs by damaging the enzyme conformation.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - January 13, 2021 Category: Hematology Source Type: research

Severe Acute Hemolytic Transfusion Reaction Treated with Ruxolitinib and Plasma Exchange
Conclusion: Ruxolitinib may be life-saving in patients with ABO-incompatible transfusion reaction which follows a severe and catastrophic course.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - January 8, 2021 Category: Hematology Source Type: research

Comparison of Total Immunoglobulin G in Ante- and Postmortem Blood Samples from Tissue Donors
Conclusion: Total IgG values in antemortem samples were below the lower limit of 6 g/L in 20% of the cases. Total IgG was significantly lower in the postmortem samples compared to the antemortem samples, while 57% were still above the lower limit. No correlation with the postmortem time could be found. This lowered IgG levels should be payed attention to when using postmortem blood for infectious serology testing. Additional NAT testing should be considered.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - January 5, 2021 Category: Hematology Source Type: research

Using the World Apheresis Association Registry Helps to Improve the Treatment Quality of Therapeutic Apheresis
Conclusion: Collection of data from TA procedures within a multinational and multicenter concept facilitates the improvement of treatment by enabling the analysis of and feedback on indications, procedures, effects, and side effects.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - January 5, 2021 Category: Hematology Source Type: research

Ethical Issues and Management of Fetal Hemolytic Anemia Caused by Anti-Rh17 in a Multipara with Rare –D– Phenotype
Conclusion: Our case shows that the risk for hemolytic complications increases with the number of pregnancies of sensitized women. Only people who also lack CcEe antigens are compatible as donors. The role of such rare donors as lifesavers, their freedom, and voluntariness conflict with the urgent need for compatible blood.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - January 5, 2021 Category: Hematology Source Type: research

Population-Based Analysis of the Impact of Demographics on the Current and Future Blood Supply in the Saarland
Conclusion: Our results on RBC demand in SL are comparable but not identical to those projected for the region of MV in eastern Germany. Due to the ongoing demographic changes in Germany as a whole, regular regional monitoring of RBC demand and the age structure of blood recipients and donors should be implemented to allow for better strategic planning in blood transfusion services and hospitals.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - December 23, 2020 Category: Hematology Source Type: research

Algorithms for the Testing of Tissue Donors for Human Immunodeficiency Virus, Hepatitis B Virus, and Hepatitis C Virus
Conclusion: In HBV and HCV testing, a high-sensitivity virus genome test should play a central role in diagnostics, especially in the case of equivocal serology, and it should be the basis for the decision to release the tissue. The proposed test algorithms and decisions are also based on current European recommendations and standards for safety and quality assurance in tissue and cell banking.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - December 22, 2020 Category: Hematology Source Type: research

Urgent Need to Regulate Convalescent Plasma Differently from Thawed Plasma
Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - December 21, 2020 Category: Hematology Source Type: research

Validation of Microbiological Testing of Tissue Preparations with Different Incubation Temperatures
Conclusion: The requirements according to the Ph. Eur. for a successful method suitability test could be fulfilled for the semiautomated blood culture bottle system with the BACTECTM FX unit for the media and microorganisms used. In the presented validation study 35 °C was shown to be the incubation temperature with the fastest growth, of the majority of the test strains used, and complete detection within 14 days.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - December 16, 2020 Category: Hematology Source Type: research

Factors Associated with Intention to Donate Hematopoietic Stem Cells among Blood Donors
Conclusions: The findings can be used to formulate a better strategy in promoting HSC donation among blood donors in the region.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - December 11, 2020 Category: Hematology Source Type: research

Contents Vol. 47, 2020
Transfus Med Hemother 2020;47:I –VI (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - December 3, 2020 Category: Hematology Source Type: research

Acknowledgement to Reviewers
Transfus Med Hemother 2020;47:472 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - December 3, 2020 Category: Hematology Source Type: research

PharmaNews
Transfus Med Hemother 2020;47:473 –474 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - December 1, 2020 Category: Hematology Source Type: research

The Immune-Modulatory Properties of iPSC-Derived Antigen-Presenting Cells
Antigen-presenting cells (APCs), such as dendritic cells (DCs) and macrophages, are important regulators of the immune system, as they connect the innate and adaptive immunity by critically regulating T-cell responses. Thus, APCs are involved in both tissue homeostasis and tolerance, but also coordinate immune responses in case of infection and inflammation. Primary APCs are commonly generated from peripheral blood-derived monocytes and have been used as cell therapeutics in several (pre-)clinical settings, e.g., immune oncology, however, with varying efficiency. One promising alternative to study antigen presentation in v...
Source: Transfusion Medicine and Hemotherapy - November 16, 2020 Category: Hematology Source Type: research

AIM Platform: A Novel Nano Artificial Antigen-Presenting Cell-Based Clinical System Designed to Consistently Produce Multi-Antigen-Specific T-Cell Products with Potent and Durable Anti-Tumor Properties
Over the last decade, tremendous progress has been made in the field of adoptive cell therapy. The two prevailing modalities include endogenous non-engineered approaches and genetically engineered T-cell approaches. Endogenous non-engineered approaches include dendritic cell-based systems and tumor-infiltrating lymphocytes (TIL) that are used to produce multi-antigen-specific T-cell products. Genetically engineered approaches, such as T-cell receptor engineered cells and chimeric antigen receptor T cells are used to produce single antigen-specific T-cell products. It is noted by the authors that there are alternative metho...
Source: Transfusion Medicine and Hemotherapy - November 16, 2020 Category: Hematology Source Type: research

The Manual Polybrene Method Can Be Used for the Pretransfusion Test to Mitigate Daratumumab Interference without a Supplementary Antiglobulin Test
Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - November 13, 2020 Category: Hematology Source Type: research

Antigen-Presenting Cells: Potential of Proven und New Players in Immune Therapies
Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - November 10, 2020 Category: Hematology Source Type: research

Functional Testing for Tranexamic Acid Duration of Action Using Modified Viscoelastometry
Conclusions: Determination of antifibrinolytic activity using the TPA test is feasible, and individual fibrinolytic capacity, e.g., in critically ill patients, can potentially be measured. This is of interest since TXA-induced lysis inhibition varies depending on kidney function.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - November 9, 2020 Category: Hematology Source Type: research

Dendritic Cells of Leukemic Origin: Specialized Antigen-Presenting Cells as Potential Treatment Tools for Patients with Myeloid Leukemia
The prognosis of elderly patients with acute myeloid leukemia (AML) and high-grade myelodysplastic syndrome (MDS) is limited due to the lack of therapy options and high relapse rates. Dendritic cell (DC)-based immunotherapy seems to be a promising treatment tool. DC are potent antigen-presenting cells and play a pivotal role on the interface of the innate and the adaptive immune system. Myeloid leukemia blasts can be converted to DC of leukemic origin (DCleu), expressing costimulatory molecules along with the whole leukemic antigen repertoire of individual patients. These generated DCleu are potent stimulators of various i...
Source: Transfusion Medicine and Hemotherapy - November 5, 2020 Category: Hematology Source Type: research

Recent Advances in Good Manufacturing Practice-Grade Generation of Dendritic Cells
Dendritic cells (DCs) are pivotal regulators of immune responses, specialized in antigen presentation and bridging the gap between the innate and adaptive immune system. Due to these key features, DCs have become a pillar of the continuously growing field of cellular therapies. Here we review recent advances in good manufacturing practice strategies and their individual specificities in relation to DC production for clinical applications. These take into account both small-scale experimental approaches as well as automated systems for patient care.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - October 28, 2020 Category: Hematology Source Type: research

Cornea Procurement and Processing up to 72 Hours: No Risk for Cornea Transplant Quality
Conclusion: All corneas finally selected for transplantation must meet the specified quality parameters. Since this naturally also applies to transplants that could only be procured at later time points, it can be concluded that DPT up to 72 h for corneal tissue is adequate and has no influence on the quality of corneas that are ultimately transplanted.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - October 8, 2020 Category: Hematology Source Type: research

News
Transfus Med Hemother 2020;47:426 –427 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - October 2, 2020 Category: Hematology Source Type: research

News
Transfus Med Hemother 2020;47:425 –425 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - October 2, 2020 Category: Hematology Source Type: research

Metabolomics and Proteomics Reveal the Variation of Substances in Apheresis Platelets during Storage and Their Effects on Cancer Cell Proliferation
Conclusion: The variant terminal-stored AP supernatants may inhibit the proliferation of cancer cells but the variant terminal AP exosomes have no effect on cancer cell proliferation.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - September 25, 2020 Category: Hematology Source Type: research

RBC Transfusion Triggers: Is There Anything New?
For many years, in daily clinical practice, the traditional 10/30 rule (hemoglobin 10 g/dL – hematocrit 30%) has been the most commonly used trigger for blood transfusions. Over the years, this approach is believed to have contributed to a countless number of unnecessary transfusions and an unknown number of overtransfusion-related deaths. Recent studies have shown that lower hemoglobin levels can safely be accepted, even in critically ill patients. However, even these new transfusion thresholds are far beyond the theoretical limits of individual anemia tolerance. For this reason, almost all publications addressing t...
Source: Transfusion Medicine and Hemotherapy - September 24, 2020 Category: Hematology Source Type: research

Leukocyte-Reactive Antibodies in Female Blood Donors: The Austrian Experience
Discussion: Leukocyte-reactive antibody screening is advised in Austrian female donors for safe blood transfusion, including single-donor convalescent plasma treatment of COVID-19 that may be implemented soon. For the introduction of LabScreen Multi, the combination with GAT should be considered to ensure correct anti-HNA-3a detection.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - September 23, 2020 Category: Hematology Source Type: research

Characteristics of Recipients of Red Blood Cell Concentrates in a German Federal State
Conclusion: Transfusion demand in a population could largely be driven by patients with high transfusion demand. Different treatment practices in this group of patients probably add to the major differences in transfusion demand per 1,000 individuals between countries. The available data cannot prove this hypothesis. Implementation of a diagnosis-related group-based monitoring system is urgently needed to allow informative monitoring on the population level and meaningful comparisons between transfusion practices.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - September 22, 2020 Category: Hematology Source Type: research

Plasma Transfusion Practice in Adult Surgical Patients: Systematic Review of the Literature
Background: Plasma transfusions are most commonly used therapeutically for bleeding or prophylactically in non-bleeding patients prior to invasive procedures or surgery. Although plasma transfusions generally seem to decline, plasma usage for indications that lack evidence of efficacy prevail.Summary: There is wide international, interinstitutional, and interindividual variance regarding the compliance with guidelines based on published references, supported by appropriate testing. There is furthermore a profound lack of evidence from randomized controlled trials comparing the effect of plasma transfusion with that of othe...
Source: Transfusion Medicine and Hemotherapy - September 18, 2020 Category: Hematology Source Type: research

Changes in Transfusion Practice in Children and Adolescents over Time
Conclusion: Transfusion rates are declining in children and adolescents, but the reasons for increases in transfusion rates in other groups are unclear. Prospective studies to evaluate transfusion rates and triggers in children are urgently needed.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - September 16, 2020 Category: Hematology Source Type: research

Cryostorage to What End? – Autologous Stem Cell Products in Burkitt Lymphoma, Acute Lymphoblastic Leukemia, Acute Myeloid Leukemia, and Myeloproliferative Neoplasm Patients
Conclusions: As the role and efficacy of autologous HDCT/ABSCT are not established in the analyzed entities, the indication for PBSC collection should be reanalyzed in regular intervals. Moreover, PBSC grafts from patients who have deceased, have insufficient grafts, or have already undergone an allogeneic TPL should be considered for disposal or (if applicable) for research use, to economize storage costs on a rational basis.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - September 15, 2020 Category: Hematology Source Type: research

53. Jahrestagung der Deutschen Gesellschaft f ür Transfusionsmedizin und Immunhämatologie (DGTI), 16.–18. September 2020, DIGITAL, Abstracts
Transfus Med Hemother 2020;47:1 –82 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - September 10, 2020 Category: Hematology Source Type: research

Long-Term Course of Haemoglobin and Ferritin Values in High-Frequency Donors of Whole Blood and Double Erythrocyte Apheresis
Conclusions: High-intensity male donors with an interdonation interval of 12 weeks for WB donation and 24 weeks for 2RBC apheresis maintain acceptable Hb levels and, after an initial decline, stable ferritin levels despite ongoing blood donation.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - August 5, 2020 Category: Hematology Source Type: research

News
Transfus Med Hemother 2020;47:344 –345 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - July 30, 2020 Category: Hematology Source Type: research

A Systematic Review of Interventions Used to Increase Blood Donor Compliance with Deferral Criteria
Conclusion: This review identified several interventions to improve donor compliance that have been tested in blood donation settings and provided evidence for the effectiveness of computerized interviews in improving detection of risk factors.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - July 23, 2020 Category: Hematology Source Type: research

Hemolysis Pathways during Storage of Erythrocytes and Inter-Donor Variability in Erythrocyte Morphology
Conclusion: RBCs can lyse without significant echinocyte formation. Lower degrees of spiculation are not a good indicator of low hemolysis when RBCs from different donors are compared.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - July 16, 2020 Category: Hematology Source Type: research

Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation
Conclusions: Our findings demonstrate that quantitative differences in EV yield might be due to equipment- and operator-dependent technical variability in ultracentrifugation-based EV isolation. Furthermore, our study emphasizes the need to standardize technical parameters such as the exact run speed and k-factor in order to transfer protocols between different laboratories. This hints at substantial inter-laboratory biases that should be assessed in multi-centric studies.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - July 8, 2020 Category: Hematology Source Type: research

News
Transfus Med Hemother 2020;47:275 –278 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - June 5, 2020 Category: Hematology Source Type: research

Extracorporeal Photopheresis: A Case of Immunotherapy Ahead of Its Time
Extracorporeal photopheresis (ECP) is a cell-based immunotherapy that involves the reinfusion of autologous leukocytes after exposure to psoralen and UVA. The treatment has been used for over 30 years, at first on patients with cutaneous T-cell lymphoma (CTCL) and later for the management of patients with graft-versus-host disease (GvHD), sclerosing disorders, atopic dermatitis, and other diseases that may share the common driving factor of a pathogenic T-cell clone or clones in blood circulation. Patients with clinical improvement mount an antigen-specific immune response that may have tolerance traits in the case of GvHD...
Source: Transfusion Medicine and Hemotherapy - May 27, 2020 Category: Hematology Source Type: research

Extracorporeal Photopheresis in Graft-versus-Host Disease
Background and Summary: Extracorporeal photopheresis (ECP) is a leukapheresis-based procedure used in the therapy of acute and chronic graft-versus-host disease (aGvHD, cGvHD) and other diseases. Based on the substantial efficacy and the excellent safety profile in the absence of immunosuppression ECP has established itself as a major treatment form for steroid-refractory GvHD. Here we review the current literature on ECP as a treatment option for patients with aGvHD as well as cGvHD.Key Messages: ECP is a well-established second-line therapy for cGvHD. Its role in the treatment of aGvHD is less clear but also points towar...
Source: Transfusion Medicine and Hemotherapy - May 19, 2020 Category: Hematology Source Type: research

Extracorporeal Phototherapy
Transfus Med Hemother 2020;47:197 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 11, 2020 Category: Hematology Source Type: research

Outcome of Extracorporeal Photopheresis as an Add-On Therapy for Antibody-Mediated Rejection in Lung Transplant Recipients
Conclusions: ECP is associated with a reduction of dnDSA in lung transplant recipients affected by AMR. Prospective studies are warranted to confirm the beneficial effects of ECP in the setting of AMR.Transfus Med Hemother 2020;47:205 –213 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 5, 2020 Category: Hematology Source Type: research

News
Transfus Med Hemother 2020;47:193 –196 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - April 7, 2020 Category: Hematology Source Type: research

Emicizumab: Hoher Blutungschutz in der Routineprophylaxe
Transfus Med Hemother 2020;47:190 –192 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - April 7, 2020 Category: Hematology Source Type: research

Does Offline Beat Inline Treatment: Investigation into Extracorporeal Photopheresis
Conclusions: The offline procedure, as established in our department, provides more apoptotic cells for treatment. The increased number of mononuclear cells collected outweighs a slightly reduced apoptosis rate after 24 h in comparison to the inline procedure. Besides this, the final decision for one or the other procedure has to take into account additional aspects, such as peripheral white blood cell count, hematocrit, and weight of the patient, required before apheresis, extracorporeal volume, and, last but not least, overall costs. The final criterion, however, has to be the reported clinical efficacy of the system app...
Source: Transfusion Medicine and Hemotherapy - March 27, 2020 Category: Hematology Source Type: research

Liberal Transfusion versus Restrictive Transfusion and Outcomes in Critically Ill Adults: A Meta-Analysis
Conclusions: In this study, we found that the restrictive red-cell transfusion strategy potentially reduced in-hospital mortality in critically ill adults with anemia compared with the liberal strategy.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - March 20, 2020 Category: Hematology Source Type: research

Thromboelastography Detects Possible Coagulation Disturbance in Pediatric Patients with Portal Cavernoma
Conclusion: TEG analysis showed that patients with PC were in a hypercoagulable state that could be partially masked by thrombocytopenia secondary to splenomegaly and hypersplenism in these patients, which indicates that our current prophylaxis and therapy regimen could be improved.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - July 3, 2019 Category: Hematology Source Type: research