News
Transfus Med Hemother 2020;47:426 –427 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - October 2, 2020 Category: Hematology Source Type: research

News
Transfus Med Hemother 2020;47:425 –425 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - October 2, 2020 Category: Hematology Source Type: research

Metabolomics and Proteomics Reveal the Variation of Substances in Apheresis Platelets during Storage and Their Effects on Cancer Cell Proliferation
Conclusion: The variant terminal-stored AP supernatants may inhibit the proliferation of cancer cells but the variant terminal AP exosomes have no effect on cancer cell proliferation.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - September 25, 2020 Category: Hematology Source Type: research

RBC Transfusion Triggers: Is There Anything New?
For many years, in daily clinical practice, the traditional 10/30 rule (hemoglobin 10 g/dL – hematocrit 30%) has been the most commonly used trigger for blood transfusions. Over the years, this approach is believed to have contributed to a countless number of unnecessary transfusions and an unknown number of overtransfusion-related deaths. Recent studies have shown that lower hemoglobin levels can safely be accepted, even in critically ill patients. However, even these new transfusion thresholds are far beyond the theoretical limits of individual anemia tolerance. For this reason, almost all publications addressing t...
Source: Transfusion Medicine and Hemotherapy - September 24, 2020 Category: Hematology Source Type: research

Leukocyte-Reactive Antibodies in Female Blood Donors: The Austrian Experience
Discussion: Leukocyte-reactive antibody screening is advised in Austrian female donors for safe blood transfusion, including single-donor convalescent plasma treatment of COVID-19 that may be implemented soon. For the introduction of LabScreen Multi, the combination with GAT should be considered to ensure correct anti-HNA-3a detection.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - September 23, 2020 Category: Hematology Source Type: research

Characteristics of Recipients of Red Blood Cell Concentrates in a German Federal State
Conclusion: Transfusion demand in a population could largely be driven by patients with high transfusion demand. Different treatment practices in this group of patients probably add to the major differences in transfusion demand per 1,000 individuals between countries. The available data cannot prove this hypothesis. Implementation of a diagnosis-related group-based monitoring system is urgently needed to allow informative monitoring on the population level and meaningful comparisons between transfusion practices.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - September 22, 2020 Category: Hematology Source Type: research

Plasma Transfusion Practice in Adult Surgical Patients: Systematic Review of the Literature
Background: Plasma transfusions are most commonly used therapeutically for bleeding or prophylactically in non-bleeding patients prior to invasive procedures or surgery. Although plasma transfusions generally seem to decline, plasma usage for indications that lack evidence of efficacy prevail.Summary: There is wide international, interinstitutional, and interindividual variance regarding the compliance with guidelines based on published references, supported by appropriate testing. There is furthermore a profound lack of evidence from randomized controlled trials comparing the effect of plasma transfusion with that of othe...
Source: Transfusion Medicine and Hemotherapy - September 18, 2020 Category: Hematology Source Type: research

Changes in Transfusion Practice in Children and Adolescents over Time
Conclusion: Transfusion rates are declining in children and adolescents, but the reasons for increases in transfusion rates in other groups are unclear. Prospective studies to evaluate transfusion rates and triggers in children are urgently needed.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - September 16, 2020 Category: Hematology Source Type: research

Cryostorage to What End? – Autologous Stem Cell Products in Burkitt Lymphoma, Acute Lymphoblastic Leukemia, Acute Myeloid Leukemia, and Myeloproliferative Neoplasm Patients
Conclusions: As the role and efficacy of autologous HDCT/ABSCT are not established in the analyzed entities, the indication for PBSC collection should be reanalyzed in regular intervals. Moreover, PBSC grafts from patients who have deceased, have insufficient grafts, or have already undergone an allogeneic TPL should be considered for disposal or (if applicable) for research use, to economize storage costs on a rational basis.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - September 15, 2020 Category: Hematology Source Type: research

53. Jahrestagung der Deutschen Gesellschaft f ür Transfusionsmedizin und Immunhämatologie (DGTI), 16.–18. September 2020, DIGITAL, Abstracts
Transfus Med Hemother 2020;47:1 –82 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - September 10, 2020 Category: Hematology Source Type: research

Long-Term Course of Haemoglobin and Ferritin Values in High-Frequency Donors of Whole Blood and Double Erythrocyte Apheresis
Conclusions: High-intensity male donors with an interdonation interval of 12 weeks for WB donation and 24 weeks for 2RBC apheresis maintain acceptable Hb levels and, after an initial decline, stable ferritin levels despite ongoing blood donation.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - August 5, 2020 Category: Hematology Source Type: research

News
Transfus Med Hemother 2020;47:344 –345 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - July 30, 2020 Category: Hematology Source Type: research

A Systematic Review of Interventions Used to Increase Blood Donor Compliance with Deferral Criteria
Conclusion: This review identified several interventions to improve donor compliance that have been tested in blood donation settings and provided evidence for the effectiveness of computerized interviews in improving detection of risk factors.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - July 23, 2020 Category: Hematology Source Type: research

Hemolysis Pathways during Storage of Erythrocytes and Inter-Donor Variability in Erythrocyte Morphology
Conclusion: RBCs can lyse without significant echinocyte formation. Lower degrees of spiculation are not a good indicator of low hemolysis when RBCs from different donors are compared.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - July 16, 2020 Category: Hematology Source Type: research

Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation
Conclusions: Our findings demonstrate that quantitative differences in EV yield might be due to equipment- and operator-dependent technical variability in ultracentrifugation-based EV isolation. Furthermore, our study emphasizes the need to standardize technical parameters such as the exact run speed and k-factor in order to transfer protocols between different laboratories. This hints at substantial inter-laboratory biases that should be assessed in multi-centric studies.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - July 8, 2020 Category: Hematology Source Type: research

News
Transfus Med Hemother 2020;47:275 –278 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - June 5, 2020 Category: Hematology Source Type: research

Extracorporeal Photopheresis: A Case of Immunotherapy Ahead of Its Time
Extracorporeal photopheresis (ECP) is a cell-based immunotherapy that involves the reinfusion of autologous leukocytes after exposure to psoralen and UVA. The treatment has been used for over 30 years, at first on patients with cutaneous T-cell lymphoma (CTCL) and later for the management of patients with graft-versus-host disease (GvHD), sclerosing disorders, atopic dermatitis, and other diseases that may share the common driving factor of a pathogenic T-cell clone or clones in blood circulation. Patients with clinical improvement mount an antigen-specific immune response that may have tolerance traits in the case of GvHD...
Source: Transfusion Medicine and Hemotherapy - May 27, 2020 Category: Hematology Source Type: research

Extracorporeal Photopheresis in Graft-versus-Host Disease
Background and Summary: Extracorporeal photopheresis (ECP) is a leukapheresis-based procedure used in the therapy of acute and chronic graft-versus-host disease (aGvHD, cGvHD) and other diseases. Based on the substantial efficacy and the excellent safety profile in the absence of immunosuppression ECP has established itself as a major treatment form for steroid-refractory GvHD. Here we review the current literature on ECP as a treatment option for patients with aGvHD as well as cGvHD.Key Messages: ECP is a well-established second-line therapy for cGvHD. Its role in the treatment of aGvHD is less clear but also points towar...
Source: Transfusion Medicine and Hemotherapy - May 19, 2020 Category: Hematology Source Type: research

Extracorporeal Phototherapy
Transfus Med Hemother 2020;47:197 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 11, 2020 Category: Hematology Source Type: research

Outcome of Extracorporeal Photopheresis as an Add-On Therapy for Antibody-Mediated Rejection in Lung Transplant Recipients
Conclusions: ECP is associated with a reduction of dnDSA in lung transplant recipients affected by AMR. Prospective studies are warranted to confirm the beneficial effects of ECP in the setting of AMR.Transfus Med Hemother 2020;47:205 –213 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 5, 2020 Category: Hematology Source Type: research

News
Transfus Med Hemother 2020;47:193 –196 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - April 7, 2020 Category: Hematology Source Type: research

Emicizumab: Hoher Blutungschutz in der Routineprophylaxe
Transfus Med Hemother 2020;47:190 –192 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - April 7, 2020 Category: Hematology Source Type: research

Does Offline Beat Inline Treatment: Investigation into Extracorporeal Photopheresis
Conclusions: The offline procedure, as established in our department, provides more apoptotic cells for treatment. The increased number of mononuclear cells collected outweighs a slightly reduced apoptosis rate after 24 h in comparison to the inline procedure. Besides this, the final decision for one or the other procedure has to take into account additional aspects, such as peripheral white blood cell count, hematocrit, and weight of the patient, required before apheresis, extracorporeal volume, and, last but not least, overall costs. The final criterion, however, has to be the reported clinical efficacy of the system app...
Source: Transfusion Medicine and Hemotherapy - March 27, 2020 Category: Hematology Source Type: research

Liberal Transfusion versus Restrictive Transfusion and Outcomes in Critically Ill Adults: A Meta-Analysis
Conclusions: In this study, we found that the restrictive red-cell transfusion strategy potentially reduced in-hospital mortality in critically ill adults with anemia compared with the liberal strategy.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - March 20, 2020 Category: Hematology Source Type: research

Thromboelastography Detects Possible Coagulation Disturbance in Pediatric Patients with Portal Cavernoma
Conclusion: TEG analysis showed that patients with PC were in a hypercoagulable state that could be partially masked by thrombocytopenia secondary to splenomegaly and hypersplenism in these patients, which indicates that our current prophylaxis and therapy regimen could be improved.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - July 3, 2019 Category: Hematology Source Type: research

Age-Related Modulations in Erythrocytes under Blood Bank Conditions
In conclusion, the efficacy of stored blood depends on the ratio of young cells in the population. This study highlights the prospects of storing young erythrocytes for a prolonged shelf life of blood.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - July 2, 2019 Category: Hematology Source Type: research

Validation of Microbiological Testing of Cellular Medicinal Products Containing Antibiotics
Conclusions: Culturing cellular products in the presence of antibiotics can serve as an effective method to reduce contamination risk but only if the chosen antibiotics neither have any influence on specifications of the investigational medicinal product nor interfere with microbiological tests. Consequently, cells and tissues primarily contaminated with microorganisms, like placenta, may be considered as a source of cellular therapeutics when cultured for a sufficient time with antibiotics and tested with a validated method. The choice of microorganisms for the validation of the microbiological test should always consider...
Source: Transfusion Medicine and Hemotherapy - July 2, 2019 Category: Hematology Source Type: research

Rhesus D Antigenic Determinants on Residual Red Blood Cells in Apheresis and Buffy Coat Platelet Concentrates
Conclusions: Processing methods influence the quantity and nature of contaminating residual RBCs and RBC-derived particles in PCs. The estimation of residual RBCs in these blood products is generally based on measurements of intact RBCs, which might underestimate the risk for alloim ­munization in transfused patients. The question of whether these RBC-derived particles can produce an immune response and, thus, should then be taken into consideration for Rh immune prophylactic treatments, remains to be clarified.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - June 27, 2019 Category: Hematology Source Type: research

Meetings and Conferences
Transfus Med Hemother 2019;46:220 –220 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - June 4, 2019 Category: Hematology Source Type: research

News
Transfus Med Hemother 2019;46:216 –219 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - June 4, 2019 Category: Hematology Source Type: research

New Approaches to Cryopreservation of Cells, Tissues, and Organs
In this concept article, we outline a variety of new approaches that have been conceived to address some of the remaining challenges for developing improved methods of biopreservation. This recognizes a true renaissance and variety of complimentary, high-potential approaches leveraging inspiration by nature, nanotechnology, the thermodynamics of pressure, and several other key fields. Development of an organ and tissue supply chain that can meet the healthcare demands of the 21st century means overcoming twin challenges of (1) having enough of these lifesaving resources and (2) having the means to store and transport them ...
Source: Transfusion Medicine and Hemotherapy - June 4, 2019 Category: Hematology Source Type: research

Effects and Mechanisms of Vitamin C Post-Conditioning on Platelet Activation after Hypoxia/Reoxygenation
Conclusions: VC inhibited platelet aggregation by blocking apoptosis. Thus, VC may have applications in the treatment of platelet-related diseases.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 28, 2019 Category: Hematology Source Type: research

Human Brucellosis: Risks and Prevalence among Iranian Blood Donors Residing in Endemic Areas
Conclusion: The observed data show a somewhat noticeable prevalence among Iranian blood donors, bringing attention to the importance of pre-donation screening via a questionnaire which supplies answers about occupational history, and any history of exposure or past infections. Further studies to evaluate the frequency and related risk factors in certain populations, in conjunction with implementing stricter regulations in blood donor selection in endemic areas, may be necessary.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 28, 2019 Category: Hematology Source Type: research

The Sting of Rejection: Deferring Blood Donors due to Low Hemoglobin Values Reduces Future Returns
Conclusion: LHD have detrimental impacts on donor return, especially if they occur repeatedly – suggesting that avoiding false LHD and helping donors to better cope with them helps to maintain the pool of prospective donors.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 27, 2019 Category: Hematology Source Type: research

Recommendations regarding Practical DEL Typing Strategies for Serologically D-Negative Asian Donors
Conclusions: Two practical DEL typing strategies using RhCE phenotyping as an initial screening method were recommended for serologically D-negative Asian donors. Compared with DEL typing usingRHD genotyping, serological DEL typing using adsorption-elution test is predicted to increase the incidence of anti-D alloimmunization and decrease the D-negative donor pool without having any cost-competitiveness but can be used in laboratories where molecular methods are not applicable.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 17, 2019 Category: Hematology Source Type: research

To Win the Battle, First Know Your Enemy: Error Rates in Immunohematology External Quality Assessment Results
Conclusion: Risk assessment should consider that error rates in pretransfusion test results vary. These data delineate the error risk potential of individual laboratory tests and thus should aid in tailoring appropriate improvement measures.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 17, 2019 Category: Hematology Source Type: research

Assessment of Time-Dependent Platelet Activation Using Extracellular Vesicles, CD62P Exposure, and Soluble Glycoprotein V Content of Platelet Concentrates with Two Different Platelet Additive Solutions
Novel analytical measures are needed to accurately monitor the properties of platelet concentrates (PCs). Since activated platelets produce platelet-derived extracellular vesicles (EVs), analyzing EVs of PCs may provide additional information about the condition of platelets. The prospect of using EVs as an auxiliary measure of platelet activation state was investigated by examining the effect of platelet additive solutions (PASs) on EV formation and platelet activation during PC storage. The time-dependent activation of platelets in PCs with PAS-B or with the further developed PAS-E was compared by measuring the exposure ...
Source: Transfusion Medicine and Hemotherapy - May 7, 2019 Category: Hematology Source Type: research

Science First: Cool Is Beautiful
Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 6, 2019 Category: Hematology Source Type: research

Aberrant ABO B Phenotype with Irregular Anti-B Caused by a Para-Bombay < b > < i > FUT1 < /i > < /b > Mutation
Conclusion: While a functionalB allele was found, analysis ofFUT1 andFUT2 genes revealed the presence of a rare para-Bombay genotype OhB. Interestingly, no anti-H but irregular anti-B was found in the patient ’s plasma, responsible for the positive cross-match with group B RBCs. Even though very rare and not reported for the European population, the presence of an H-deficient phenotype should be considered when investigating individuals with an unusual ABO blood group type.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 5, 2019 Category: Hematology Source Type: research

Potential of Parameters of Iron Metabolism for the Diagnosis of Anemia of Inflammation in the Critically Ill
Conclusions: In this explorative study on the use of different parameters of iron metabolism for diagnosing AI during an ICU stay, low levels of commonly measured markers such as plasma iron, transferrin, and transferrin saturation have the highest sensitivity and specificity and outperform ferritin and hepcidin.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 5, 2019 Category: Hematology Source Type: research

Impact of Regular Whole-Blood Donation on Body Iron Stores
Conclusion: The present study demonstrates iron depletion in regular voluntary blood donors. In 11.2% of the donors, iron deficiency erythropoiesis was noted. A ferritin screening after the first donation followed up at the tenth donation might help detect iron-deficient individuals. Iron supplementation for all regular blood donors and female donors in particular will help prevent the shrinking donor pool due to iron deficiency.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 5, 2019 Category: Hematology Source Type: research

Organ Preservation into the 2020s: The Era of Dynamic Intervention
Organ preservation has been of major importance ever since transplantation developed into a global clinical activity. The relatively simple procedures were developed on a basic comprehension of low-temperature biology as related to organs outside the body. In the past decade, there has been a significant increase in knowledge of the sequelae of effects in preserved organs, and how dynamic intervention by perfusion can be used to mitigate injury and improve the quality of the donated organs. The present review focuses on (1) new information about the cell and molecular events impacting on ischemia/reperfusion injury during ...
Source: Transfusion Medicine and Hemotherapy - May 5, 2019 Category: Hematology Source Type: research

Cryopreservation of Human Ovarian Tissue: A Review
Background: Cryopreservation of human ovarian tissue has been increasingly applied worldwide to safeguard fertility in cancer patients, notably in young girls and women who cannot delay the onset of their treatment. Moreover, it has been proposed to patients with benign pathologies with a risk of premature ovarian insufficiency. So far, more than 130 live births have been reported after transplantation of cryopreserved ovarian tissue, and almost all patients recovered their ovarian function after tissue reimplantation.Summary: This review aims to summarize the recent results described in the literature regarding human ovar...
Source: Transfusion Medicine and Hemotherapy - April 15, 2019 Category: Hematology Source Type: research

News
Transfus Med Hemother 2019;46:124 –127 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - April 15, 2019 Category: Hematology Source Type: research

Meetings and Conferences
Transfus Med Hemother 2019;46:129 –130 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - April 15, 2019 Category: Hematology Source Type: research

Comprehensive Molecular Analysis of Serologically D-Negative and Weak/Partial D Phenotype in Thai Blood Donors
Discussion: For the first time, a comprehensive overview of the nature and distribution of variantRHD alleles in Thailand is reported. It is a milestone to pave the way towards improvement of the current screening strategy to identify DEL donors accurately. The next step will be the design and implementation of a simple molecular test for screening the most frequent alleles, specifically in this population.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - April 3, 2019 Category: Hematology Source Type: research

Risk Factors for Transfusion after Orthotopic Liver Transplantation
Conclusion: Our study demonstrated an association between intraoperative transfusion and warm ischemia time with postoperative transfusion requirements. The identification of risk factors for transfusion in the postoperative period may improve management of these patients by increasing awareness to bleeding complications in this high-risk population and by expanding hemostasis monitoring to the postoperative period.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - April 3, 2019 Category: Hematology Source Type: research

Evaluation for Perioperative Blood Transfusion during Major Abdominal Procedures in a Pediatric Population: A Retrospective Observation Cohort Study
Conclusions: Overtransfusion was common in hepatic resection and younger age, but to definitely prove this hypothesis, a prospective randomized trial needs to be performed.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - March 31, 2019 Category: Hematology Source Type: research

The Near Future of Vitrification of Oocytes and Embryos: Looking into Past Experience and Planning into the Future
Currently vitrification is the method of choice for low-temperature preservation of oocytes and embryos. However, that was not the case until about 10 years ago when freezing methods were used relatively successfully for embryos and investigated (unsuccessfully) for oocyte preservation. In this paper we will review the history of oocyte and embryo cryopreservation and look into ways and methods for overcoming and improving the vitrification method since it suffers from inherent disadvantages since it is a cumbersome, time-consuming and costly procedure.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - March 31, 2019 Category: Hematology Source Type: research

Age of Red Cells for Transfusion and Outcomes in Critically Ill Patients: A Meta-Analysis
Conclusions: The study concluded that age of red cells for transfusion did not affect the outcomes in critically ill patients.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - March 31, 2019 Category: Hematology Source Type: research