Thromboelastography Detects Possible Coagulation Disturbance in Pediatric Patients with Portal Cavernoma
Conclusion: TEG analysis showed that patients with PC were in a hypercoagulable state that could be partially masked by thrombocytopenia secondary to splenomegaly and hypersplenism in these patients, which indicates that our current prophylaxis and therapy regimen could be improved.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - July 3, 2019 Category: Hematology Source Type: research

Age-Related Modulations in Erythrocytes under Blood Bank Conditions
In conclusion, the efficacy of stored blood depends on the ratio of young cells in the population. This study highlights the prospects of storing young erythrocytes for a prolonged shelf life of blood.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - July 2, 2019 Category: Hematology Source Type: research

Validation of Microbiological Testing of Cellular Medicinal Products Containing Antibiotics
Conclusions: Culturing cellular products in the presence of antibiotics can serve as an effective method to reduce contamination risk but only if the chosen antibiotics neither have any influence on specifications of the investigational medicinal product nor interfere with microbiological tests. Consequently, cells and tissues primarily contaminated with microorganisms, like placenta, may be considered as a source of cellular therapeutics when cultured for a sufficient time with antibiotics and tested with a validated method. The choice of microorganisms for the validation of the microbiological test should always consider...
Source: Transfusion Medicine and Hemotherapy - July 2, 2019 Category: Hematology Source Type: research

Rhesus D Antigenic Determinants on Residual Red Blood Cells in Apheresis and Buffy Coat Platelet Concentrates
Conclusions: Processing methods influence the quantity and nature of contaminating residual RBCs and RBC-derived particles in PCs. The estimation of residual RBCs in these blood products is generally based on measurements of intact RBCs, which might underestimate the risk for alloim ­munization in transfused patients. The question of whether these RBC-derived particles can produce an immune response and, thus, should then be taken into consideration for Rh immune prophylactic treatments, remains to be clarified.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - June 27, 2019 Category: Hematology Source Type: research

Meetings and Conferences
Transfus Med Hemother 2019;46:220 –220 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - June 4, 2019 Category: Hematology Source Type: research

News
Transfus Med Hemother 2019;46:216 –219 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - June 4, 2019 Category: Hematology Source Type: research

New Approaches to Cryopreservation of Cells, Tissues, and Organs
In this concept article, we outline a variety of new approaches that have been conceived to address some of the remaining challenges for developing improved methods of biopreservation. This recognizes a true renaissance and variety of complimentary, high-potential approaches leveraging inspiration by nature, nanotechnology, the thermodynamics of pressure, and several other key fields. Development of an organ and tissue supply chain that can meet the healthcare demands of the 21st century means overcoming twin challenges of (1) having enough of these lifesaving resources and (2) having the means to store and transport them ...
Source: Transfusion Medicine and Hemotherapy - June 4, 2019 Category: Hematology Source Type: research

Effects and Mechanisms of Vitamin C Post-Conditioning on Platelet Activation after Hypoxia/Reoxygenation
Conclusions: VC inhibited platelet aggregation by blocking apoptosis. Thus, VC may have applications in the treatment of platelet-related diseases.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 28, 2019 Category: Hematology Source Type: research

Human Brucellosis: Risks and Prevalence among Iranian Blood Donors Residing in Endemic Areas
Conclusion: The observed data show a somewhat noticeable prevalence among Iranian blood donors, bringing attention to the importance of pre-donation screening via a questionnaire which supplies answers about occupational history, and any history of exposure or past infections. Further studies to evaluate the frequency and related risk factors in certain populations, in conjunction with implementing stricter regulations in blood donor selection in endemic areas, may be necessary.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 28, 2019 Category: Hematology Source Type: research

The Sting of Rejection: Deferring Blood Donors due to Low Hemoglobin Values Reduces Future Returns
Conclusion: LHD have detrimental impacts on donor return, especially if they occur repeatedly – suggesting that avoiding false LHD and helping donors to better cope with them helps to maintain the pool of prospective donors.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 27, 2019 Category: Hematology Source Type: research

Recommendations regarding Practical DEL Typing Strategies for Serologically D-Negative Asian Donors
Conclusions: Two practical DEL typing strategies using RhCE phenotyping as an initial screening method were recommended for serologically D-negative Asian donors. Compared with DEL typing usingRHD genotyping, serological DEL typing using adsorption-elution test is predicted to increase the incidence of anti-D alloimmunization and decrease the D-negative donor pool without having any cost-competitiveness but can be used in laboratories where molecular methods are not applicable.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 17, 2019 Category: Hematology Source Type: research

To Win the Battle, First Know Your Enemy: Error Rates in Immunohematology External Quality Assessment Results
Conclusion: Risk assessment should consider that error rates in pretransfusion test results vary. These data delineate the error risk potential of individual laboratory tests and thus should aid in tailoring appropriate improvement measures.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 17, 2019 Category: Hematology Source Type: research

Assessment of Time-Dependent Platelet Activation Using Extracellular Vesicles, CD62P Exposure, and Soluble Glycoprotein V Content of Platelet Concentrates with Two Different Platelet Additive Solutions
Novel analytical measures are needed to accurately monitor the properties of platelet concentrates (PCs). Since activated platelets produce platelet-derived extracellular vesicles (EVs), analyzing EVs of PCs may provide additional information about the condition of platelets. The prospect of using EVs as an auxiliary measure of platelet activation state was investigated by examining the effect of platelet additive solutions (PASs) on EV formation and platelet activation during PC storage. The time-dependent activation of platelets in PCs with PAS-B or with the further developed PAS-E was compared by measuring the exposure ...
Source: Transfusion Medicine and Hemotherapy - May 7, 2019 Category: Hematology Source Type: research

Science First: Cool Is Beautiful
Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 6, 2019 Category: Hematology Source Type: research

Aberrant ABO B Phenotype with Irregular Anti-B Caused by a Para-Bombay < b > < i > FUT1 < /i > < /b > Mutation
Conclusion: While a functionalB allele was found, analysis ofFUT1 andFUT2 genes revealed the presence of a rare para-Bombay genotype OhB. Interestingly, no anti-H but irregular anti-B was found in the patient ’s plasma, responsible for the positive cross-match with group B RBCs. Even though very rare and not reported for the European population, the presence of an H-deficient phenotype should be considered when investigating individuals with an unusual ABO blood group type.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 5, 2019 Category: Hematology Source Type: research

Potential of Parameters of Iron Metabolism for the Diagnosis of Anemia of Inflammation in the Critically Ill
Conclusions: In this explorative study on the use of different parameters of iron metabolism for diagnosing AI during an ICU stay, low levels of commonly measured markers such as plasma iron, transferrin, and transferrin saturation have the highest sensitivity and specificity and outperform ferritin and hepcidin.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 5, 2019 Category: Hematology Source Type: research

Impact of Regular Whole-Blood Donation on Body Iron Stores
Conclusion: The present study demonstrates iron depletion in regular voluntary blood donors. In 11.2% of the donors, iron deficiency erythropoiesis was noted. A ferritin screening after the first donation followed up at the tenth donation might help detect iron-deficient individuals. Iron supplementation for all regular blood donors and female donors in particular will help prevent the shrinking donor pool due to iron deficiency.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - May 5, 2019 Category: Hematology Source Type: research

Organ Preservation into the 2020s: The Era of Dynamic Intervention
Organ preservation has been of major importance ever since transplantation developed into a global clinical activity. The relatively simple procedures were developed on a basic comprehension of low-temperature biology as related to organs outside the body. In the past decade, there has been a significant increase in knowledge of the sequelae of effects in preserved organs, and how dynamic intervention by perfusion can be used to mitigate injury and improve the quality of the donated organs. The present review focuses on (1) new information about the cell and molecular events impacting on ischemia/reperfusion injury during ...
Source: Transfusion Medicine and Hemotherapy - May 5, 2019 Category: Hematology Source Type: research

Cryopreservation of Human Ovarian Tissue: A Review
Background: Cryopreservation of human ovarian tissue has been increasingly applied worldwide to safeguard fertility in cancer patients, notably in young girls and women who cannot delay the onset of their treatment. Moreover, it has been proposed to patients with benign pathologies with a risk of premature ovarian insufficiency. So far, more than 130 live births have been reported after transplantation of cryopreserved ovarian tissue, and almost all patients recovered their ovarian function after tissue reimplantation.Summary: This review aims to summarize the recent results described in the literature regarding human ovar...
Source: Transfusion Medicine and Hemotherapy - April 15, 2019 Category: Hematology Source Type: research

News
Transfus Med Hemother 2019;46:124 –127 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - April 15, 2019 Category: Hematology Source Type: research

Meetings and Conferences
Transfus Med Hemother 2019;46:129 –130 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - April 15, 2019 Category: Hematology Source Type: research

Comprehensive Molecular Analysis of Serologically D-Negative and Weak/Partial D Phenotype in Thai Blood Donors
Discussion: For the first time, a comprehensive overview of the nature and distribution of variantRHD alleles in Thailand is reported. It is a milestone to pave the way towards improvement of the current screening strategy to identify DEL donors accurately. The next step will be the design and implementation of a simple molecular test for screening the most frequent alleles, specifically in this population.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - April 3, 2019 Category: Hematology Source Type: research

Risk Factors for Transfusion after Orthotopic Liver Transplantation
Conclusion: Our study demonstrated an association between intraoperative transfusion and warm ischemia time with postoperative transfusion requirements. The identification of risk factors for transfusion in the postoperative period may improve management of these patients by increasing awareness to bleeding complications in this high-risk population and by expanding hemostasis monitoring to the postoperative period.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - April 3, 2019 Category: Hematology Source Type: research

Evaluation for Perioperative Blood Transfusion during Major Abdominal Procedures in a Pediatric Population: A Retrospective Observation Cohort Study
Conclusions: Overtransfusion was common in hepatic resection and younger age, but to definitely prove this hypothesis, a prospective randomized trial needs to be performed.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - March 31, 2019 Category: Hematology Source Type: research

The Near Future of Vitrification of Oocytes and Embryos: Looking into Past Experience and Planning into the Future
Currently vitrification is the method of choice for low-temperature preservation of oocytes and embryos. However, that was not the case until about 10 years ago when freezing methods were used relatively successfully for embryos and investigated (unsuccessfully) for oocyte preservation. In this paper we will review the history of oocyte and embryo cryopreservation and look into ways and methods for overcoming and improving the vitrification method since it suffers from inherent disadvantages since it is a cumbersome, time-consuming and costly procedure.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - March 31, 2019 Category: Hematology Source Type: research

Age of Red Cells for Transfusion and Outcomes in Critically Ill Patients: A Meta-Analysis
Conclusions: The study concluded that age of red cells for transfusion did not affect the outcomes in critically ill patients.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - March 31, 2019 Category: Hematology Source Type: research

Technical Considerations in the Freezing, Low-Temperature Storage and Thawing of Stem Cells for Cellular Therapies
The commercial and clinical development of cellular therapy products will invariably require cryopreservation and frozen storage of cellular starting materials, intermediates and/or final product. Optimising cryopreservation is as important as optimisation of the cell culture process in obtaining maximum yield and a consistent end-product. Suboptimal cryopreservation can lead not only to batch-to-batch variation, lowered cellular functionality and reduced cell yield, but also to the potential selection of subpopulations with genetic or epigenetic characteristics divergent from the original cell line. Regulatory requirement...
Source: Transfusion Medicine and Hemotherapy - March 31, 2019 Category: Hematology Source Type: research

Expression of the < b > < i > GBGT1 < /i > < /b > Gene and the Forssman Antigen in Red Blood Cells in a Palestinian Population
Conclusion: Our results confirmed the allelic diversity ofGBGT1 and identified a novel nucleotide polymorphism in this gene, p.Arg243Cys. Our results also confirmed that the FORS blood group system is a low-frequency system.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - March 31, 2019 Category: Hematology Source Type: research

Microbiological Screening of Platelet Concentrates in Europe
The risk of transfusion-associated sepsis due to transmission of bacteria is a persistent problem in the transfusion field. Despite numerous interventions to reduce the risk, cases of bacterial sepsis following transfusion are repeatedly being reported. Especially platelet concentrates are highly susceptible to bacterial contaminations due to the growth-promoting storage conditions. In Europe, blood establishments and national authorities have implemented individual precaution measures to mitigate the risk of bacterial transmission. To obtain an overview of the different approaches, we compiled information from national au...
Source: Transfusion Medicine and Hemotherapy - March 20, 2019 Category: Hematology Source Type: research

Emicizumab in the Treatment of Acquired Haemophilia: A Case Report
The prognosis of acquired haemophilia A (AHA) is severe and treatment options are limited. Emicizumab is a novel bispecific humanized monoclonal antibody in the treatment of inherited AHA with inhibitors. An 83-year-old AHA patient with congestive heart failure and a high risk for thromboembolic and cardiac events who had initially been treated successfully with steroids and substitution of recombinant B-domain-deleted porcine FVIII developed severe bleeding complications and a secondary increase in inhibitor titres after 4 weeks of treatment. Conventional therapeutic strategies failed, and the patient was subsequently tre...
Source: Transfusion Medicine and Hemotherapy - March 16, 2019 Category: Hematology Source Type: research

Viral Metagenomics of Blood Donors and Blood-Derived Products Using Next-Generation Sequencing
Transfusion-transmitted infections remain a permanent threat in medicine. It keeps the burden of the past, marked by serious infections transmitted by transfusion, and is constantly threatened by emerging viruses. The global rise of immunosuppression among patients undergoing frequent transfusions exacerbates this problem. Over the past decade, criteria for donor selection have become increasingly more stringent. Although routine nucleic acid testing (NAT) for virus-specific detection has become more sensitive, these safety measures are only valuable for a limited number of select viruses. The scientific approach to this i...
Source: Transfusion Medicine and Hemotherapy - March 16, 2019 Category: Hematology Source Type: research

Daratumumab Interference in Pretransfusion Testing Is Overcome by Addition of Daratumumab Fab Fragments to Patients ’ Plasma
Conclusion: We present a quite easy, reproducible, and cost-effective method for DARA-Fab fragment preparation. Blocking CD38 epitopes with DARA-Fab easily overcomes DARA interference in pretransfusion testing without affecting alloantibody detection.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - March 14, 2019 Category: Hematology Source Type: research

Safe-Testing Algorithm for Individual-Donation Nucleic Acid Testing: 10 Years of Experience in a Low-Prevalence Country
Conclusions: The algorithm to deal with IR ID-NAT donations using duplicate repeat testing is very safe and cost-effective in low-prevalence countries. There is no unnecessary destruction of blood products, no counseling of false reactive donors, and also no need to add further complexity to the screening algorithm.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - March 14, 2019 Category: Hematology Source Type: research

Blood Donor Screening for Hepatitis E Virus in the European Union
We report a range of HEV-RNA reactivity rates from 1 in 744 donations (France) to 1 in 8,636 donations (Wales) with an overall EU rate of 1 in 3,109 donations (3.2 million donations screened). HEV genotypes 3c, 3e, and 3f are the most frequently reported subtypes. In these 8 countries, both universal (n = 5) and selective (n = 3) screening policies have been introduced utilising either individual donation (ID;n = 1) or mini-pool (MP;n = 7; MP-6, -16, -24, and -96) testing. We also describe the Irish experience of HEV screening utilising an ID-NAT-based donor screening algorithm which intercepts donations even from those wi...
Source: Transfusion Medicine and Hemotherapy - March 14, 2019 Category: Hematology Source Type: research

Extension of the Storage Period of Platelet Concentrates in Germany to 5 Days by Bacterial Testing: Is it Worth the Effort?
The shelf life of platelet concentrates (PCs) was limited in Germany to 4 days after the day of production because platelet-related septic complications had been observed, mainly with PCs at the end of their shelf life. The reduction in PC shelf life gives rise to several problems, including an increased number of discarded products, accompanied by potential challenges of how to ensure an adequate supply of platelet products. The aim of this study was to show routine experience over the last 7 years using the Bactiflow (BF) assay to screen for bacterial contamination late in PC storage, followed by extension of PC shelf li...
Source: Transfusion Medicine and Hemotherapy - March 14, 2019 Category: Hematology Source Type: research

Infectious Screening of Blood Components: Is There Still a Need for Further Inventions?
Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - March 14, 2019 Category: Hematology Source Type: research

Hematopoietic Chimera in a Male Blood Donor and His Dizygotic Twin Sister
Twin hematopoietic chimera in humans is a phenomenon that was discovered accidentally and the prevalence of which remains unclear. The resolution of chimera cases requires studying family medical records, data analysis, and investigations of hematopoietic cells and cells from other tissues. The interactions among ABO, Lewis, and secretor histo-blood group systems are explored to resolve cases of hematopoietic chimera. Here we report a rare case of hematopoietic chimera where twins present a mixed field reaction in the ABO, Rh, and Kidd red blood cell phenotyping. Using red blood cells separated from the mixed field as well...
Source: Transfusion Medicine and Hemotherapy - March 7, 2019 Category: Hematology Source Type: research

Depletion of αβ+ T and B Cells Using the CliniMACS Prodigy: Results of 10 Graft-Processing Procedures from Haploidentical Donors
Conclusion: Overall, we found the TCR αβ and CD19 depletion procedure on the CliniMACS Prodigy easy to handle and reliable, providing reproducible good results.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - March 7, 2019 Category: Hematology Source Type: research

Effect of Platelet Transfusion on Clot Strength in Dengue Fever with Thrombocytopenia Related Bleeding: A Thromboelastography-Based Study
This study assessed the impact of platelet transfusion on the control of bleeding and improvement in clot strength as assessed by thromboelastography among 74 patients with dengue. The effect of either weight-based random donor platelets or 1 unit of single donor aphaeresis units was studied. Transfusion of weight-based random donor platelets resulted in a mean platelet count increase of 10,210 cells per mm3 at 24 h from pretransfusion values, which reached marginal statistical significance (p = 0.031). Patients who received single donor platelets achieved a mean platelet increment of 22,874 cells per mm3 at 24 h, and the ...
Source: Transfusion Medicine and Hemotherapy - March 7, 2019 Category: Hematology Source Type: research

Red Blood Cells Derived from Whole Blood Treated with Riboflavin and UV Light Maintain Adequate Cell Quality through 21 Days of Storage
Conclusion: RBCs derived from WB treated with the Mirasol system meet accepted FDA guidelines for RBC quality through 21 days of storage at 1 –6°C.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - February 22, 2019 Category: Hematology Source Type: research

Implementation of Molecular < b > < i > RHD < /i > < /b > Typing at Two Blood Transfusion Institutes from Southeastern Europe
Conclusion: Rh molecular testing was successfully implemented in both blood transfusion institutes in Banja Luka and Belgrade. This study proved the efficiency of serological algorithms for weak D, as well as the presence of the RHD gene among serologically tested RhD-negative, C/E-positive samples.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - February 15, 2019 Category: Hematology Source Type: research

News
Transfus Med Hemother 2019;46:56 –57 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - February 8, 2019 Category: Hematology Source Type: research

News
Transfus Med Hemother 2019;46:58 –60 (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - February 8, 2019 Category: Hematology Source Type: research

History and Future of Nucleic Acid Amplification Technology Blood Donor Testing
The introduction of blood donor screening by virus nucleic acid amplification technology (NAT) in the mid to late 1990s was driven by the so-called AIDS and hepatitis C virus (HCV) epidemic, with thousands of recipients of infected blood products and components. Plasma fractionators were the first to introduce NAT testing besides pathogen reduction procedures, to reduce the virus transmission risk through their products. To achieve a similar safety standard, NAT was then also introduced for labile blood components. German transfusion centres were the first to start in-house NAT testing of their donations in pools of up to ...
Source: Transfusion Medicine and Hemotherapy - February 6, 2019 Category: Hematology Source Type: research

Cryopreservation of Hematopoietic Stem Cells: Emerging Assays, Cryoprotectant Agents, and Technology to Improve Outcomes
Hematopoietic stem cell (HSC) therapy is widely used to treat a growing number of hematological and non-hematological diseases. Cryopreservation of HSCs allows for cells to be transported from the site of processing to the site of clinical use, creates a larger window of time in which cells can be administered to patients, and allows sufficient time for quality control and regulatory testing. Currently, HSCs and other cell therapies conform to the same cryopreservation techniques as cells used for research purposes: cells are cryopreserved in dimethyl sulfoxide (DMSO) at a slow cooling rate. As a result, HSC therapy can re...
Source: Transfusion Medicine and Hemotherapy - February 6, 2019 Category: Hematology Source Type: research

Chimeric Antigen Receptor T Cells: A Race to Revolutionize Cancer Therapy
For years, cancer treatment was dominated by chemotherapy, radiation therapy, and stem cell transplantation. New insights into genetic characteristics of leukemic cells have initiated the development of the chimeric antigen receptor (CAR) T-cell therapy. This type of adoptive cell immunotherapy has been a breakthrough in the treatment of aggressive B-cell lymphoma and B-cell precursor acute lymphoblastic leukemia. In August 2018, the European Commission has approved the first CAR T-cell products – tisagenlecleucel (Kymriah®, Novartis) and axicabtagene ciloleucel (Yescarta®, Gilead) – for hematological n...
Source: Transfusion Medicine and Hemotherapy - February 6, 2019 Category: Hematology Source Type: research

Trends in Plasma Toll Fractionation for Self Sufficiency of Plasma-Derived Medicinal Products in Italy
Conclusions: Competition among companies resulted in higher yields of albumin and immunoglobulin, decreasing their level of market dependence, an increase in the portfolio of medicines, and a decrease in price due to fractionation (20 –30%), thus improving perspectives of the national program for self-sufficiency in plasma-derived medicinal products.Transfus Med Hemother (Source: Transfusion Medicine and Hemotherapy)
Source: Transfusion Medicine and Hemotherapy - February 6, 2019 Category: Hematology Source Type: research

CAR-Expressing Natural Killer Cells for Cancer Retargeting
Since the approval in 2017 and the outstanding success of Kymriah ® and Yescarta®, the number of clinical trials investigating the safety and efficacy of chimeric antigen receptor-modified autologous T cells has been constantly rising. Currently, more than 200 clinical trials are listed on clinicaltrial.gov. In contrast to CAR-T cells, natural killer (NK) cells can be used from allogeneic donors as an “off the shelf product” and provide alternative candidates for cancer retargeting. This review summarises preclinical results of CAR-engineered NK cells using both primary human NK cells and the cell line ...
Source: Transfusion Medicine and Hemotherapy - February 5, 2019 Category: Hematology Source Type: research

Clinical Use of Mesenchymal Stromal Cells in the Treatment of Acute Graft-versus-Host Disease
Acute graft-versus-host disease (aGvHD) continues to impact morbidity and mortality after allogeneic stem cell transplantation (allo-SCT). First-line therapy for aGvHD still remains the use of high-dose corticosteroids. Unfortunately, 40 –60% of patients with aGvHD exhibit steroid resistance, which is associated with a very poor prognosis. As no effective second-line therapy existed, in recent decades various treatment options were considered for the treatment of therapy-refractory GvHD. Based on their in vitro immunomodulatory pr operties, the use of mesenchymal stromal cells (MSCs) in the treatment of aGvHD has bee...
Source: Transfusion Medicine and Hemotherapy - February 5, 2019 Category: Hematology Source Type: research

B Cell-Based Cancer Immunotherapy
B cells are not only producers of antibodies, but also contribute to immune regulation or act as potent antigen-presenting cells. The potential of B cells for cellular therapy is still largely underestimated, despite their multiple diverse effector functions. The CD40L/CD40 signaling pathway is the most potent activator of antigen presentation capacity in B lymphocytes. CD40-activated B cells are potent antigen-presenting cells that induce specific T-cell responses in vitro and in vivo. In preclinical cancer models in mice and dogs, CD40-activated B cell-based cancer immunotherapy was able to induce effective antitumor imm...
Source: Transfusion Medicine and Hemotherapy - February 5, 2019 Category: Hematology Source Type: research