Attention-optimized DeepLab V3  + for automatic estimation of cucumber disease severity
Automatic and accurate estimation of disease severity is critical for disease management and yield loss prediction. Conventional disease severity estimation is performed using images with simple backgrounds, w... (Source: Plant Methods)
Source: Plant Methods - September 6, 2022 Category: Biology Authors: Kaiyu Li, Lingxian Zhang, Bo Li, Shufei Li and Juncheng Ma Tags: Research Source Type: research

Interest of phenomic prediction as an alternative to genomic prediction in grapevine
Phenomic prediction has been defined as an alternative to genomic prediction by using spectra instead of molecular markers. A reflectance spectrum provides information on the biochemical composition within a t... (Source: Plant Methods)
Source: Plant Methods - September 5, 2022 Category: Biology Authors: Charlotte Brault, Juliette Lazerges, Agn ès Doligez, Miguel Thomas, Martin Ecarnot, Pierre Roumet, Yves Bertrand, Gilles Berger, Thierry Pons, Pierre François, Loïc Le Cunff, Patrice This and Vincent Segura Tags: Research Source Type: research

The mechanisms and prediction of non-structural carbohydrates accretion and depletion after mechanical wounding in slash pine (Pinus elliottii) using near-infrared reflectance spectroscopy
The allocation of non-structural carbohydrates (NSCs) plays a critical role in the physiology and metabolism of tree growth and survival defense. However, little is known about the allocation of NSC after cont... (Source: Plant Methods)
Source: Plant Methods - September 1, 2022 Category: Biology Authors: Yanjie Li, Honggang Sun, Thiago de Paula Prot ásio, Paulo Ricardo Gherardi Hein and Baoguo Du Tags: Research Source Type: research

Cowpea speed breeding using regulated growth chamber conditions and seeds of oven-dried immature pods potentially accommodates eight generations per year
Cowpea is a dryland crop with potential to improve food security in sub-Saharan Africa, where it is mostly produced and consumed. Contemporary plant improvement technologies, including genome editing, marker-a... (Source: Plant Methods)
Source: Plant Methods - August 29, 2022 Category: Biology Authors: Offiong Ukpong Edet and Takayoshi Ishii Tags: Methodology Source Type: research

An efficient CRISPR-Cas9 enrichment sequencing strategy for characterizing complex and highly duplicated genomic regions. A case study in the Prunus salicina LG3-MYB10 genes cluster
Genome complexity is largely linked to diversification and crop innovation. Examples of regions with duplicated genes with relevant roles in agricultural traits are found in many crops. In both duplicated and ... (Source: Plant Methods)
Source: Plant Methods - August 27, 2022 Category: Biology Authors: Arnau Fiol, Federico Jurado-Ruiz, Elena L ópez‑Girona and Maria José Aranzana Tags: Methodology Source Type: research

Correction to: Laser ‑based molecular delivery and its applications in plant science
(Source: Plant Methods)
Source: Plant Methods - August 25, 2022 Category: Biology Authors: Dag Heinemann, Miroslav Zabic, Mitsuhiro Terakawa and Jens Boch Tags: Correction Source Type: research

Automated, image-based disease measurement for phenotyping resistance to soybean frogeye leaf spot
Frogeye leaf spot is a disease of soybean, and there are limited sources of crop genetic resistance. Accurate quantification of resistance is necessary for the discovery of novel resistance sources, which can ... (Source: Plant Methods)
Source: Plant Methods - August 16, 2022 Category: Biology Authors: Samuel C. McDonald, James Buck and Zenglu Li Tags: Methodology Source Type: research

Synchrotron tomography applications in agriculture and food sciences research: a review
This article introduces the characteristics of synchrotron X-ray imaging and its applications in agriculture and food science res... (Source: Plant Methods)
Source: Plant Methods - August 13, 2022 Category: Biology Authors: Navnath S. Indore, Chithra Karunakaran and Digvir S. Jayas Tags: Review Source Type: research

A method of two-dimensional correlation spectroscopy combined with residual neural network for comparison and differentiation of medicinal plants raw materials superior to traditional machine learning: a case study on Eucommia ulmoides leaves
Eucommia ulmoides leaf (EUL), as a medicine and food homology plant, is a high-quality industrial raw material with great development potential for a valuable economic crop. There are many factors affecting the q... (Source: Plant Methods)
Source: Plant Methods - August 13, 2022 Category: Biology Authors: Lian Li, Zhi Min Li and Yuan Zhong Wang Tags: Research Source Type: research

Unsupervised analysis of NIRS spectra to assess complex plant traits: leaf senescence as a use case
As a rapid and non-destructive method, Near Infrared Spectroscopy is classically proposed to assess plant traits in many scientific fields, to observe enlarged genotype panels and to document the temporal kine... (Source: Plant Methods)
Source: Plant Methods - August 12, 2022 Category: Biology Authors: H éloïse Villesseche, Martin Ecarnot, Elsa Ballini, Ryad Bendoula, Nathalie Gorretta and Pierre Roumet Tags: Research Source Type: research

Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa
Genome editing is essential for crop molecular breeding. However, gene editing in turnip (Brassica rapa var. rapa) have not been reported owing to the very low transformation efficiency. (Source: Plant Methods)
Source: Plant Methods - August 6, 2022 Category: Biology Authors: Yuanyuan Liu, Li Zhang, Cheng Li, Yunqiang Yang, Yuanwen Duan, Yongping Yang and Xudong Sun Tags: Research Source Type: research

DeLTa-Seq: direct-lysate targeted RNA-Seq from crude tissue lysate
Quantification of gene expression such as RNA-Seq is a popular approach to study various biological phenomena. Despite the development of RNA-Seq library preparation methods and sequencing platforms in the las... (Source: Plant Methods)
Source: Plant Methods - August 6, 2022 Category: Biology Authors: Makoto Kashima, Mari Kamitani, Yasuyuki Nomura, Natsumi Mori-Moriyama, Shigeyuki Betsuyaku, Hiromi Hirata and Atsushi J. Nagano Tags: Methodology Source Type: research

An integrated isotopic labeling and freeze sampling apparatus (ILSA) to support sampling leaf metabolomics at a centi-second scale
Photosynthesis close interacts with respiration and nitrogen assimilation, which determine the photosynthetic efficiency of a leaf. Accurately quantifying the metabolic fluxes in photosynthesis, respiration an... (Source: Plant Methods)
Source: Plant Methods - July 30, 2022 Category: Biology Authors: Qiming Tang, Qingfeng Song, Xiaoxiang Ni, Zai Shi, Genyun Chen and Xinguang Zhu Tags: Methodology Source Type: research

Earbox, an open tool for high-throughput measurement of the spatial organization of maize ears and inference of novel traits
Characterizing plant genetic resources and their response to the environment through accurate measurement of relevant traits is crucial to genetics and breeding. Spatial organization of the maize ear provides ... (Source: Plant Methods)
Source: Plant Methods - July 28, 2022 Category: Biology Authors: V. Oury, T. Leroux, O. Turc, R. Chapuis, C. Palaffre, F. Tardieu, S. Alvarez Prado, C. Welcker and S. Lacube Tags: Methodology Source Type: research

Evaluating the productivity of ancient Pu ’er tea trees (Camellia sinensis var. assamica): a multivariate modeling approach
The demand for productive economic plant resources is increasing with the continued growth of the human population. Ancient Pu ’er tea trees [Camellia sinensis var. assamica (J. W. Mast.) Kitam.] are an important ... (Source: Plant Methods)
Source: Plant Methods - July 27, 2022 Category: Biology Authors: Shuqiao Zhang, Wendou Liu, Xinmeng Cheng, Zizhi Wang, Fengjun Yuan, Wengui Wu and Shengxi Liao Tags: Research Source Type: research