3D-GBS: a universal genotyping-by-sequencing approach for genomic selection and other high-throughput low-cost applications in species with small to medium-sized genomes
Despite the increased efficiency of sequencing technologies and the development of reduced-representation sequencing (RRS) approaches allowing high-throughput sequencing (HTS) of multiplexed samples, the per-s... (Source: Plant Methods)
Source: Plant Methods - February 5, 2023 Category: Biology Authors: Maxime de Ronne, Ga étan Légaré, François Belzile, Brian Boyle and Davoud Torkamaneh Tags: Methodology Source Type: research
The rapid-tome, a 3D-printed microtome, and an updated hand-sectioning method for high-quality plant sectioning
Microscopic analysis of plant anatomy is a common procedure in biology to study structure and function that requires high-quality sections for accurate measurements. Hand sectioning of specimens is typically l... (Source: Plant Methods)
Source: Plant Methods - February 4, 2023 Category: Biology Authors: David J. Thomas, Jordan Rainbow and Laura E. Bartley Tags: Methodology Source Type: research
Quantification of the three-dimensional root system architecture using an automated rotating imaging system
Crop breeding based on root system architecture (RSA) optimization is an essential factor for improving crop production in developing countries. Identification, evaluation, and selection of root traits of soil... (Source: Plant Methods)
Source: Plant Methods - February 2, 2023 Category: Biology Authors: Qian Wu, Jie Wu, Pengcheng Hu, Weixin Zhang, Yuntao Ma, Kun Yu, Yan Guo, Jing Cao, Huayong Li, Baiming Li, Yuyang Yao, Hongxin Cao and Wenyu Zhang Tags: Methodology Source Type: research
An optimized pipeline for live imaging whole Arabidopsis leaves at cellular resolution
Live imaging is the gold standard for determining how cells give rise to organs. However, tracking many cells across whole organs over large developmental time windows is extremely challenging. In this work, w... (Source: Plant Methods)
Source: Plant Methods - February 1, 2023 Category: Biology Authors: Kate Harline and Adrienne H. K. Roeder Tags: Methodology Source Type: research
Development of an accurate low cost NDVI imaging system for assessing plant health
Spectral imaging is a key method for high throughput phenotyping that can be related to a large variety of biological parameters. The Normalised Difference Vegetation Index (NDVI), uses specific wavelengths to... (Source: Plant Methods)
Source: Plant Methods - January 30, 2023 Category: Biology Authors: John D. Stamford, Silvere Vialet-Chabrand, Iain Cameron and Tracy Lawson Tags: Methodology Source Type: research
YOLO POD: a fast and accurate multi-task model for dense Soybean Pod counting
The number of soybean pods is one of the most important indicators of soybean yield, pod counting is crucial for yield estimation, cultivation management, and variety breeding. Counting pods manually is slow a... (Source: Plant Methods)
Source: Plant Methods - January 28, 2023 Category: Biology Authors: Shuai Xiang, Siyu Wang, Mei Xu, Wenyan Wang and Weiguo Liu Tags: Research Source Type: research
Karst vegetation coverage detection using UAV multispectral vegetation indices and machine learning algorithm
Karst vegetation is of great significance for ecological restoration in karst areas. Vegetation Indices (VIs) are mainly related to plant yield which is helpful to understand the status of ecological restorati... (Source: Plant Methods)
Source: Plant Methods - January 23, 2023 Category: Biology Authors: Wen Pan, Xiaoyu Wang, Yan Sun, Jia Wang, Yanjie Li and Sheng Li Tags: Research Source Type: research
Bayesian modelling of phosphorus content in wheat grain using hyperspectral reflectance data
As a result of the technological progress, the use of sensors for crop survey has substantially increased, generating valuable information for modelling agricultural data. Plant spectroscopy jointly with stati... (Source: Plant Methods)
Source: Plant Methods - January 20, 2023 Category: Biology Authors: Rosa Angela Pacheco-Gil, Ciro Velasco-Cruz, Paulino P érez-Rodríguez, Juan Burgueño, Sergio Pérez-Elizalde, Francelino Rodrigues, Ivan Ortiz-Monasterio, David Hebert del Valle-Paniagua and Fernando Toledo Tags: Research Source Type: research
A method for rapid and homogenous initiation of post-harvest physiological deterioration in cassava storage roots identifies Indonesian cultivars with improved shelf-life performance
Cassava is the most cultivated and consumed root crop in the world. One of the major constraints to the cassava value chain is the short shelf life of cassava storage roots which is primarily due to the so-cal... (Source: Plant Methods)
Source: Plant Methods - January 18, 2023 Category: Biology Authors: Ima M. Zainuddin, Brieuc Lecart, Enny Sudarmonowati and Herv é Vanderschuren Tags: Research Source Type: research
Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis
Cotton has tremendous economic value worldwide; however, its allopolyploid nature and time-consuming transformation methods have hampered the development of cotton functional genomics. The protoplast system ha... (Source: Plant Methods)
Source: Plant Methods - January 18, 2023 Category: Biology Authors: Ke Zhang, Shanhe Liu, Yunze Fu, Zixuan Wang, Xiubo Yang, Wenjing Li, Caihua Zhang, Dongmei Zhang and Jun Li Tags: Methodology Source Type: research
The semi-automated development of plant cell wall finite element models
This study presents a methodology for a high-throughput digitization and quantification process of plant cell walls characterization, including the automated development of two-dimensional finite element model... (Source: Plant Methods)
Source: Plant Methods - January 9, 2023 Category: Biology Authors: Andrew Sayad, Yusuf Oduntan, Norbert Bokros, Seth DeBolt, Alice Benzecry, Daniel J. Robertson and Christopher J. Stubbs Tags: Methodology Source Type: research
Spatial and Texture Analysis of Root System distribution with Earth mover ’s Distance (STARSEED)
Root system architectures are complex and challenging to characterize effectively for agronomic and ecological discovery. (Source: Plant Methods)
Source: Plant Methods - January 5, 2023 Category: Biology Authors: Joshua Peeples, Weihuang Xu, Romain Gloaguen, Diane Rowland, Alina Zare and Zachary Brym Tags: Methodology Source Type: research
NADPH containing superoxide-producing thermostable complex from raspberry, apricot, grape, and grape seeds: isolation, purification, and properties
NADPH oxidase (Nox) plays a crucial role in reactive oxygen spices (ROS) production and mediates different diseases ’ development. Under aerobic conditions, the NADPH-containing protein component of the (NCP)-F... (Source: Plant Methods)
Source: Plant Methods - January 3, 2023 Category: Biology Authors: Sona M. Feschyan, Ruzan M. Simonyan, Gegham M. Simonyan, Maxim A. Simonyan and Ashkhen L. Manukyan Tags: Research Source Type: research
Isolation, purification and PEG-mediated transient expression of mesophyll protoplasts in Camellia oleifera
Camellia oleifera (C. oleifera) is a woody edible oil crop of great economic importance. Because of the lack of modern biotechnology research, C. oleifera faces huge challenges in both breeding and basic researc... (Source: Plant Methods)
Source: Plant Methods - December 22, 2022 Category: Biology Authors: Sufang Li, Rui Zhao, Tianwen Ye, Rui Guan, Linjie Xu, Xiaoling Ma, Jiaxi Zhang, Shixin Xiao and Deyi Yuan Tags: Methodology Source Type: research
Effects of cushion box and closed let-down ladder usage on damage to corn during handling: physiological deterioration
Corn seeds have a high susceptibility to mechanical damage due to their large size and mass. The main purpose of this study was to evaluate the effects of the cushion box and closed let-down ladder usage in mi... (Source: Plant Methods)
Source: Plant Methods - December 22, 2022 Category: Biology Authors: Reza Shahbazi and Feizollah Shahbazi Tags: Research Source Type: research
