An efficient grafting method for phytoplasma transmission in Catharanthus roseus
Phytoplasmas are parasitic plant pathogens that reside intracellularly within the sieve tube cells. Phytoplasmas induce various symptoms, including floral virescence, phyllody, leaf yellowing, and witches ’-bro... (Source: Plant Methods)
Source: Plant Methods - January 20, 2024 Category: Biology Authors: Ho-Chun Chang and Jen-Chih Chen Tags: Methodology Source Type: research
Automatic 3D cell segmentation of fruit parenchyma tissue from X-ray micro CT images using deep learning
High quality 3D information of the microscopic plant tissue morphology —the spatial organization of cells and intercellular spaces in tissues—helps in understanding physiological processes in a wide variety of ... (Source: Plant Methods)
Source: Plant Methods - January 19, 2024 Category: Biology Authors: Leen Van Doorselaer, Pieter Verboven and Bart Nicolai Tags: Research Source Type: research
Cryopreservation of Abies alba × A. numidica and Pinus nigra embryogenic tissues by stepwise dehydration method
Cryopreservation makes it possible to preserve plant biodiversity for thousands of years in ex situ storage. The stepwise dehydration method is a simple and versatile cryopreservation technique based on the vi... (Source: Plant Methods)
Source: Plant Methods - January 17, 2024 Category: Biology Authors: Teresa Hazubska-Przyby ł, Mikołaj Krzysztof Wawrzyniak, Agata Obarska and Terezia Salaj Tags: Research Source Type: research
LeTra: a leaf tracking workflow based on convolutional neural networks and intersection over union
The study of plant photosynthesis is essential for productivity and yield. Thanks to the development of high-throughput phenotyping (HTP) facilities, based on chlorophyll fluorescence imaging, photosynthetic t... (Source: Plant Methods)
Source: Plant Methods - January 17, 2024 Category: Biology Authors: Federico Jurado-Ruiz, Thu-Phuong Nguyen, Joseph Peller, Mar ía José Aranzana, Gerrit Polder and Mark G. M. Aarts Tags: Methodology Source Type: research
Exploring genetic variability under extended photoperiod in lentil (Lens Culinaris Medik): vegetative and phenological differentiation according to genetic material ’s origins
Lentil is an important pulse that contributes to global food security and the sustainability of farming systems. Hence, it is important to increase the production of this crop, especially in the context of cli... (Source: Plant Methods)
Source: Plant Methods - January 13, 2024 Category: Biology Authors: Mohammed Mitache, Aziz Baidani, Bouchaib Bencharki and Omar Idrissi Tags: Research Source Type: research
Assessing data analysis techniques in a high-throughput meiosis-like induction detection system
Strategies to understand meiotic processes have relied on cytogenetic and mutant analysis. However, thus far in vitro meiosis induction is a bottleneck to laboratory-based plant breeding as factor(s) that swit... (Source: Plant Methods)
Source: Plant Methods - January 12, 2024 Category: Biology Authors: Tanner M. Cook, Eva Biswas, Somak Dutta, Siddique I. Aboobucker, Sara Hazinia and Thomas L übberstedt Tags: Methodology Source Type: research
Genomic prediction for root and yield traits of barley under a water availability gradient: a case study comparing different spatial adjustments
In drought periods, water use efficiency depends on the capacity of roots to extract water from deep soil. A semi-field phenotyping facility (RadiMax) was used to investigate above-ground and root traits in sp... (Source: Plant Methods)
Source: Plant Methods - January 12, 2024 Category: Biology Authors: Biructawit B. Tessema, Miguel A. Raffo, Xiangyu Guo, Simon F. Svane, Lene Krusell, Jens Due Jensen, Anja Karine Ruud, Marta Malinowska, Kristian Thorup-Kristensen and Just Jensen Tags: Research Source Type: research
Cucumber mosaic virus-induced gene and microRNA silencing in water dropwort (Oenanthe javanica (Blume) DC)
Water dropwort (Oenanthe javanica (Blume) DC), an aquatic perennial plant from the Apiaceae family, rich in dietary fibert, vitamins, and minerals. It usually grows in wet soils and water. Despite accumulating th... (Source: Plant Methods)
Source: Plant Methods - January 11, 2024 Category: Biology Authors: Zhen He, Shuangyu Sheng, Lingqi Wang, Tingting Dong, Kun Zhang and Liangjun Li Tags: Research Source Type: research
Monitoring of plant water uptake by measuring root dielectric properties on a fine timescale: diurnal changes and response to leaf excision
The measurement of root dielectric response is a useful non-destructive method to evaluate root growth and function. Previous studies tracked root development throughout the plant growing cycle by single-time ... (Source: Plant Methods)
Source: Plant Methods - January 9, 2024 Category: Biology Authors: Imre Cseresny és, Anna Füzy, Sándor Kabos, Bettina Kelemen, Kálmán Rajkai and Tünde Takács Tags: Methodology Source Type: research
Mitochondrial DNA editing in potato through mitoTALEN and mitoTALECD: molecular characterization and stability of editing events
The aim of this study was to evaluate and characterize the mutations induced by two TALE-based approaches, double-strand break (DSB) induction by the FokI nuclease (mitoTALEN) and targeted base editing by the Ddd... (Source: Plant Methods)
Source: Plant Methods - January 5, 2024 Category: Biology Authors: Alessandro Nicolia, Nunzia Scotti, Nunzio D ’Agostino, Giovanna Festa, Lorenza Sannino, Gaetano Aufiero, Shin-ichi Arimura and Teodoro Cardi Tags: Research Source Type: research
Optimization of preparation and transformation of protoplasts from Populus simonii × P. nigra leaves and subcellular localization of the major latex protein 328 (MLP328)
Populus simonii × P. nigra is an ideal material for studying the molecular mechanisms of woody plants. In recent years, research on Populus simonii × P. nigra has increasingly focused on the application of transg... (Source: Plant Methods)
Source: Plant Methods - January 4, 2024 Category: Biology Authors: Ping Yang, Yao Sun, Xin Sun, Yao Li and Lei Wang Tags: Research Source Type: research
RPIOSL: construction of the radiation transfer model for rice leaves
The radiative transfer model of vegetation leaves simulates the transmission mechanism of light inside the vegetation and simulates the reflectivity of blades according to the change law of different component... (Source: Plant Methods)
Source: Plant Methods - January 3, 2024 Category: Biology Authors: Shuang Xiang, Zhongyu Jin, Jinpeng Li, Fenghua Yu and Tongyu Xu Tags: Research Source Type: research
A novel in-situ-process technique constructs whole circular cpDNA library
The chloroplast genome (cp genome) is directly related to the study and analysis of molecular phylogeny and evolution of plants in the phylogenomics era. The cp genome, whereas, is highly plastic and exists as... (Source: Plant Methods)
Source: Plant Methods - January 3, 2024 Category: Biology Authors: Qiang Zhou, Xianlong Ding, Hongjie Wang, Zunaira Farooq, Liang Wang and Shouping Yang Tags: Methodology Source Type: research
Gray mold and anthracnose disease detection on strawberry leaves using hyperspectral imaging
Gray mold and anthracnose are the main factors affecting strawberry quality and yield. Accurate and rapid early disease identification is of great significance to achieve precise targeted spraying to avoid lar... (Source: Plant Methods)
Source: Plant Methods - December 19, 2023 Category: Biology Authors: Baohua Zhang, Yunmeng Ou, Shuwan Yu, Yuchen Liu, Ying Liu and Wei Qiu Tags: Research Source Type: research
A highly efficient protocol for isolation of protoplast from China, Assam and Cambod types of tea plants [Camellia sinensis (L.) O. Kuntze]
Tea is the most popular beverage worldwide second only to water. Its demand is tremendously rising due to increased awareness of its medicinal importance. The quality and uses of tea depend on the tea-types wh... (Source: Plant Methods)
Source: Plant Methods - December 15, 2023 Category: Biology Authors: Abhishek Kumar, Nikhil Rawat, Shweta Thakur, Rohit Joshi and Shiv Shanker Pandey Tags: Methodology Source Type: research
